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Citations for Akoya Biosciences :
101 - 150 of 189 citations for Streptavidin ZAP Antibody Kit goat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: CODEX staining was done using the CODEX staining kit (Akoya, Cat 7000008) according to Akoya’s CODEX user manual with modifications to include a photobleaching step and overnight incubation in antibodies at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... and Opal 650 and TSA-biotin (TSA Plus Biotin Kit) (Akoya Biosciences) and streptavidin-conjugated Atto 425 (Sigma ...
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bioRxiv - Genomics 2024Quote: ... For multiplexed IHC staining the Opal 6-plex Detection Kit (Akoya Biosciences) was used ...
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bioRxiv - Physiology 2022Quote: 14 of the 23 antibodies used here were conjugated in-house using the protocol outlined by Akoya Biosciences (Table 3)19,20 ...
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bioRxiv - Molecular Biology 2021Quote: ... The binding sites of HRP-conjugated secondary antibodies were detected with Red Opal (1:100, AKOYA Biosciences) and a DAPI counterstain was applied (1:1000) ...
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bioRxiv - Cancer Biology 2021Quote: ... Multispectral immunohistochemistry was performed using the Opal 7-Color Manual IHC Kit (Akoya Biosciences/PerkinElmer ...
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bioRxiv - Cancer Biology 2021Quote: Multiplex IHC was performed using an Opal 7-Color IHC Kit (Akoya Biosciences) on a Bond RX automated stainer (Leica Biosystems) ...
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bioRxiv - Developmental Biology 2021Quote: ... Chromogenic reaction was performed with either TSA Plus Cyanine 3 Kit (Akoya Biosciences) for nkx3.1 or ImmPACT Vector Red (Vector Laboratories ...
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bioRxiv - Immunology 2022Quote: ... Staining was performed using the Opal™ 7-Color Automatic IHC Kit (Akoya) according to the manufacturer’s recommendations ...
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bioRxiv - Systems Biology 2023Quote: ... Slides were stained using Akoya Staining Kit for Phenocycler (Akoya Biosciences, Delaware, USA) according to the manufacturer’s recommended protocol ...
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bioRxiv - Cell Biology 2024Quote: CODEX staining was done using the sample kit for PhenoCycler-Fusion (Akoya, 7000017) according to Akoya’s PhenoCycler-Fusion user guide with modifications to include a photobleaching step and overnight incubation in antibodies at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: CODEX staining was done using the sample kit for PhenoCycler-Fusion (Akoya, 7000017) according to Akoya’s PhenoCycler-Fusion user guide with modifications to include a photobleaching step and overnight incubation with antibodies at 4°C ...
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bioRxiv - Immunology 2021Quote: ... RNA probes and panCK antibody were detected with the following Opal Fluorophores pairings: CXCL13 Opal 480 (Akoya FP1500001KT), CXCL10/11 Opal 520 (Akoya FP1487001KT) ...
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bioRxiv - Immunology 2022Quote: ... Each target was linked using the OPAL Polymer HRP conjugated secondary antibodies (ready-to-use, ARH1001EA, AKOYA Biosciences). Visualization of the different targets was established using the OPAL Polaris 570 (FP1488001KT ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were washed in TBS + 0.1% tween (TBS-T) and blocked in Antibody Diluent/Block (Akoya Biosciences, ARD1001EA) for 30 minutes at RT ...
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bioRxiv - Cell Biology 2022Quote: ... The binding of antibodies were detected with a 1:150 dilution of OPAL 520 Reagent (AKOYA, SKU FP1487001KT). Slides were digitally imaged using an Aperio ScanScope FL Scanner (Leica Biosystems).
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:15,000 dilution followed by secondary antibody 570 opal reagent (1:100 dilution) (Akoya Biosciences) and peak wavelength at 555/570 ...
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bioRxiv - Neuroscience 2023Quote: ... Detection of probe and antibody were performed using Tyramide Signal Amplification system with Opal 570 or 520 reagents (Akoya Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding was visualized using secondary anti-mouse/anti-rabbit polymer HRP followed with TSA-Opal reagents (Akoya NEL811001KT). Tissue slides were incubated with DAPI as a counterstain and coverslipped with VectaShield mounting media (Vector Labs) ...
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bioRxiv - Cancer Biology 2023Quote: ... Then a staining dye was added after secondary antibody washes: α-SMA – Opal-dye 520 (Akoya Biosciences OP-001001) at 1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... Multiplexed staining was performed using the Opal 6-plex detection kit (Akoya Biosciences NEL871001KT) on a Bond Rx Research Stainer (Leica Biosystems) ...
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bioRxiv - Developmental Biology 2023Quote: Fluorescent in situ hybridization was performed using the Tyramide Signal Amplification kit (Akoya Biosciences). A more detailed protocol can be found in the Supplement ...
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bioRxiv - Developmental Biology 2023Quote: ... and the TSA Plus Cyanine 3 Kit (NEL744001KT, Akoya Biosciences, Menlo Park, CA, USA) following the supplier’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... mRNA probes were detected using TSA®Plus detection kit (Akoya Biosciences, Marlborough, MA); eGFP was detected with Fluoroscein (1:2000) ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:100 dilution followed by secondary antibody 520 reagent (1:100) (Akoya Biosciences; Menlo Park, California) and peak wavelength at 494/525 ...
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bioRxiv - Genomics 2022Quote: A total of 32 antibodies were used for CODEX and 19 of them were conjugated in-house using a protocol outlined by Akoya Biosciences (Akoya Biosciences ...
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bioRxiv - Neuroscience 2022Quote: FFPE tissue sections containing OB and OT were stained consecutively with specific primary antibodies according to standard protocols provided by Akoya and performed routinely by the HIMSR ...
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bioRxiv - Pathology 2023Quote: ... secondary antibodies labeled with horseradish peroxidase (all with reduced cross-reactivity from Jackson-Immunoresearch) in combination with 7plex Opal system (Akoya) were used according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then blocked with 10% BSA + 0.05% Tween20 and the antibodies were diluted in 2% BSA in 0.05% PBST and used in a multiplexed manner with OPAL reagents (Akoya Biosciences). All primary antibodies (described above for western blotting ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then blocked with 10% BSA + 0.05% Tween20 and the antibodies were diluted in 2% BSA in 0.05% PBST and used in a multiplexed manner with OPAL reagents (Akoya Biosciences). All primary antibodies were incubated overnight at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... and probes were amplified using TSA fluorescent detection kits as directed by manufacture (Akoya Bioscience). The C1 probe was conjugated to Fluorescein ...
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bioRxiv - Cell Biology 2023Quote: ... and Opal 6-Plex Detection Kit for Whole Slide Imaging (Akoya Biosciences, Marlborough, MA, USA) were employed for the staining process ...
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bioRxiv - Cancer Biology 2023Quote: ... which was performed using an Opal 6-Plex Detection Kit (Akoya Biosciences, Menlo Park, CA) with the following Abs ...
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bioRxiv - Developmental Biology 2024Quote: Fluorescence in situ hybridization (FISH) was performed using the Tyramide Signal Amplification kit from Akoya Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: The multi-IF was performed using the Opal 6-Plex Manual Detection Kit (NEL811001KT, Akoya), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... polymer-conjugated secondary antibody for 20 min and reacted with one of the tyramide signal amplification reagents including Opal 520 (Akoya, FP1487001KT), Opal 570 (Akoya ...
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bioRxiv - Cancer Biology 2022Quote: ... The tissue was washed and equilibrated before staining for 3 hours at room temperature with the 28-plex CODEX antibody cocktail in a staining buffer containing blocking solution (Akoya Biosciences). After staining ...
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bioRxiv - Cancer Biology 2021Quote: ... was used for antibody raised in rabbit (PH3 and PHGDH) and the OPAL Polymer HRP Ms+Rb (Akoya/Perkin Elmer, ARH1001EA) was used for the antibody raised in mouse (panCK) ...
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bioRxiv - Cancer Biology 2023Quote: ... Epitope retrieval/microwave treatment (MWT) for all antibodies was performed by boiling slides in Antigen Retrieval buffer 6 (AR6 pH6; Akoya, AR6001KT). Protein blocking was performed using antibody diluent/blocking buffer (Akoya ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with HRP-conjugated anti-DIG (1:500) antibody overnight and treated with TSA-plus biotin (NEL749A001KT; Akoya Biosciences), followed by streptavidin-Alexa Fluor 488 (S32354 ...
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bioRxiv - Cell Biology 2023Quote: ... Experimental sections (4-μm thickness) and positive tissues were stained with specific primary antibodies (1:100) followed by Opal multiplex immunostaining system (Akoya Biosciences) to generate the stained slides ...
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bioRxiv - Molecular Biology 2024Quote: ... Experimental sections (4-μm thickness) and positive tissues were stained with specific primary antibodies followed by Opal multiplex immunostaining system (Akoya Biosciences) to generate the stained slides ...
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bioRxiv - Molecular Biology 2023Quote: Multispectral fluorescence staining was performed using the Opal 6-Plex Detection kit (Akoya Biosciences, Marlborough, MA) using formalin-fixed ...
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bioRxiv - Molecular Biology 2023Quote: ... the development reaction was carried out using the TSA Plus Cyanine 3 Kit (Akoya Biosciences, US) (FISH ...
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bioRxiv - Immunology 2024Quote: ... Multiplex immunofluorescence imaging was performed on FFPE sections using an Opal multiplex IHC kit (Akoya Biosciences), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Multiplexed immunohistochemistry (mIHC) assay was performed using the Opal 6-Plex Detection Kit (AKOYA #811001, USA) as described previously73 ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with Tris-NaCl-Tween Buffer after incubation with anti-DIG-POD antibodies and incubated with Cy3-tyramide solution (TSA Plus Cyanine 3; AKOYA Biosciences, NEL744001KT) for 10 min ...
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bioRxiv - Neuroscience 2021Quote: Multiplexed immunofluorescent staining was carried out using the Opal Polaris 7 color IHC detection kit (Akoya biosciences) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... using Opal 4-Color Automation IHC Kit (CK/STING/DAPI) (Cat. No. NEL820001KT, Akoya Biosciences, Marlborough, MA). According to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... paraffin-embedded whole tissue sections using the Opal 7-color fluorescence immunohistochemistry (IHC) kit (Akoya biosciences, USA), as previously described25 ...