Labshake search
Citations for Eppendorf :
51 - 90 of 90 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Microinjections utilized an Eppendorf Femtojet apparatus with Femtotips II pre-pulled glass needles (Eppendorf). All Drosophila melanogaster strains and crosses were maintained on standard medium at 25 °C unless stated otherwise ...
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bioRxiv - Cell Biology 2023Quote: ... with Femtotips II at a transmitted light microscope or using a FemtoJet microinjector (Eppendorf) mounted on an inverted Leica SP8 confocal microscope.
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bioRxiv - Cell Biology 2020Quote: ... were added to DNA-bead mix and incubated in a Thermomixer C (Eppendorf, 5382000015) at 56°C while being centrifuged at 2,000 rpm for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... was loaded with the injection mix using a Microloader™ tip (Eppendorf. Cat # 930001007). The eggs were injected on their posterior side and visually examined for slight expansion during injection to endure the transfer of the injection solution ...
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bioRxiv - Molecular Biology 2020Quote: ... Eppendorf FemtoJet® 4x programmable microinjector and Eppendorf Femtotip II injection capillaries (Eppendorf, Hamburg, Germany). The injection conditions were injection pressure (pi ...
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bioRxiv - Biochemistry 2022Quote: ... SYBR Green incorporation in cell DNA amplification was determined using the Master epRealplex cycler® (Eppendorf, France) and the following program to increase SYBR Green incorporation ...
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bioRxiv - Physiology 2021Quote: ... were evaluated using qPCR according to the manufacturer’s recommendations (EVAGREEN; Solis Byodine, EUA) and using the Master Cycler Realplex system (Eppendorf, Germany) with the following cycling conditions ...
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bioRxiv - Cell Biology 2021Quote: ... and a custom primer set mix (Fluidigm) that were amplified for 18 cycles on a thermocycler (Eppendorf). Excess primers were removed from the samples by Exonuclease I (NEB ...
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bioRxiv - Systems Biology 2021Quote: ... The six 96-well master plates were then re-arrayed into the final 384 well v-bottom PCR Plates (Eppendorf #951020702).
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bioRxiv - Microbiology 2023Quote: ... 2 μM final test concentration) were mixed (reaction mix) and transferred into a white 96 well plate (Eppendorf Twin-Tec ...
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bioRxiv - Developmental Biology 2019Quote: ... 6 day-old tapeworms were obtained and microinjected with dsRNA using femtotips II via the Femtojet injection system (Eppendorf) to obtain spreading across the first ∼ 3-4 mm anterior of the tapeworm ...
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bioRxiv - Biophysics 2020Quote: ... or 1 mM LY294,002 and 10 μg/ml Alexa594 were prepared in DB and loaded into Femtotips II (Eppendorf). The tip mounted on the micromanipulator (TransferMan 4r or TransferMan NK2 ...
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bioRxiv - Microbiology 2021Quote: ... master plates of phage communities were assembled in deep 96 well plates using a liquid handling robot (epMotion® 5070, Eppendorf, Germany) in triplicate ...
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bioRxiv - Cancer Biology 2020Quote: ... −3.6 D/V: −1.6 with 4 µl of transfection mix using a pulled glass capillary and a FemtoJet microinjector (Eppendorf). In utero electroporation was performed at day E15.5 ...
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bioRxiv - Developmental Biology 2023Quote: ... Plasmids were injected (plasmid mix for microinjection in Table S6) at the Hensen’s node of the embryos using a Microloader pipette tip (Eppendorf). Electroporation was performed using CUY21EDIT (Nepa Gene ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatants of these washes were combined with the recovered digestion mix and dried in a vacuum concentrator (Eppendorf).
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bioRxiv - Biophysics 2020Quote: ... The supernatant was used for microinjection into adherent HeLa-GFPvim cells with a microinjection system (Injectman II and Femtojet, Eppendorf), and injection parameters were 100 Pa injection pressure ...
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bioRxiv - Genetics 2021Quote: ... Microinjection took place under a non-inverted light microscope with a Femtojet 4i microinjector and Femtotip II microinjection capillaries (Eppendorf). The prepared Cas9/sgRNA mixtures were injected into the posterior end of the embryos to ensure efficient targeting of the germline.
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bioRxiv - Genomics 2020Quote: ... The resuspended and lysed nuclei were stained with 1x Hoechst and a BD FACS Aria II was used to distribute 2500 nuclei into each well of a 96-well v-bottom plate (Eppendorf twin.tec LoBind skirted 96 well PCR plate ...
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bioRxiv - Microbiology 2021Quote: ... with 100 μl aliquot of the ultra-low gelling temperature agarose gel solution (6% in Mueller Hinton Broth II, cation-adjusted) to 85 °C in a Thermomixer (Eppendorf Thermomixer R Shaker, Eppendorf). Once the temperature was reached ...
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bioRxiv - Immunology 2023Quote: ... The reagents were mixed as per the QuikChange II XL Site-Directed Mutagenesis Kit instructions and loaded into a Mastercycler pro S (Eppendorf) with the settings recommended by the manual ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Soaked biofilms were gently removed from the agar surface using a sterile Drigalski spatula and a pipette tip and together with the unabsorbed stabilization mix transferred into a 2 mL tube (Eppendorf). An additional 1 mL of stabilization buffer was added to the tubes and the content was homogenized with a sterile pestle ...
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bioRxiv - Biophysics 2022Quote: ... The aqueous solution was layered on top of the oil-surfactant mix in a volumetric ratio of 1:3 inside a microtube (Eppendorf). The tube was manually shaken for about 30 s until water-in-oil droplets formed ...
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bioRxiv - Developmental Biology 2020Quote: ... The injection solution (cRNA or CRISPR/Cas9 injection mix) was loaded into the injection needle from the back by Eppendorf Microloader and the holding pipet was purchased from Eppendorf (VacuTip).
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bioRxiv - Cell Biology 2021Quote: ... The reaction mix was incubated for 30 min at 25°C with 700 rpm shaking in a ThemoMixer C (Eppendorf) and subsequently quenched for 20 min by adding Tris-HCl (final concentration 50 mM) ...
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bioRxiv - Developmental Biology 2022Quote: ... Zygotes from super-ovulated C57BL/6N females were microinjected into the paternal pronucleus with the RNP/donor oligo mix using a Femtojet 4i microinjector (Eppendorf). After injection ...
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bioRxiv - Neuroscience 2023Quote: ... 1 nl of the mix was injected into the cell of a one-cell stage embryo using a FemtoJet Microinjector (Eppendorf).
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bioRxiv - Molecular Biology 2023Quote: ... Freshly laid zebrafish eggs were collected from breeding tanks and 1 nL solution mix was injected using a FemtoJet 4i (Eppendorf) into the yolk before the first cellular division.
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bioRxiv - Neuroscience 2023Quote: ... The beads-captured proteins were digested overnight at 37 °C with 0.5 μg trypsin/LysC mix in 25 mM ammonium bicarbonate under vigorous shaking (1200 rpm, Eppendorf Thermomixer). The supernatants were collected ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated at 37°C at 600 rpm with interval mixing (30 seconds mix, 2 minutes pause) on a Thermomixer C (2231000667, Eppendorf). After the last digestion ...
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bioRxiv - Cancer Biology 2023Quote: ... The beads-captured proteins were digested overnight at 37 ℃ with 0.5 μg trypsin/LysC mix in 25 mM ammonium bicarbonate under vigorous shaking (1200 rpm, Eppendorf Thermomixer). The supernatants were collected and the peptides were purified by a modified Sp3 clean-up protocol and finally solubilized in the mobile phase A (0.1% formic acid in water) ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR was done with HOT FIREPol EvaGreen qPCR Mix Plus (Solis Biodyne, Tartu, Estonia) on RealPlex4 thermocycler (Eppendorf, Hamburg, Germany). For miRNA detection ...
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bioRxiv - Synthetic Biology 2022Quote: ... The fluorocarbon/ethanol mix was shaken at room temperature for 16 h with 1000 rpm using an Eppendorf ThermoMixer C (Eppendorf AG, Germany) to perform the liquid-liquid extraction ...
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bioRxiv - Cell Biology 2022Quote: The beads were resuspended with 40 µL extension mix and incubated at 37°C on a PCR cycler (Eppendorf, UX-93944-39) for 10 minutes ...
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bioRxiv - Genetics 2023Quote: ... we microinjected ∼1 nL microinjection mix into each embryo (either the single cell or one of the dividing cells) using a FemtoJet 4i microinjector (Eppendorf, Hamburg, Germany) (Supplementary Figure S1) ...
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bioRxiv - Microbiology 2023Quote: ... 2 μM final test concentration) were mixed (reaction mix) and transferred into a white 96 well plate (Eppendorf Twin-Tec, item no. 0030132718, Eppendorf SE; Hamburg, Germany). For single colony samples ...
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bioRxiv - Microbiology 2021Quote: ... with 100 μl aliquot of the ultra-low gelling temperature agarose gel solution (6% in Mueller Hinton Broth II, cation-adjusted) to 85 °C in a Thermomixer (Eppendorf Thermomixer R Shaker, Eppendorf). Once the temperature was reached ...
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bioRxiv - Microbiology 2020Quote: ... Reactions and amplifications were carried out as previously described in 1x SYBR qPCR Premix Ex Taq (Tli RNaseH Plus) (Ozyme, St Quentin en Yvelines, France) mix and on a Mastercycler® ep realplex (Eppendorf, Le Pecq, Fr) (Nouri et al. ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl of the lipid-containing lower organic phase was spotted on a MALDI target plate (Prespotted AnchorChip 96 Set for Proteomics II; Bruker Daltonics/Eppendorf; washed peptide-free with 2-propanol) pre-spotted with 1 µl of 9-aminoacridine (10 mg/ml dissolved in acetone:water 9:1 (v/v) ...
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bioRxiv - Genetics 2022Quote: ... The mix was micro-injected in the gonads of young adult N2 Bristol wild types (Zeiss Axio Observer A1 with Eppendorf Femtojet and Eppendorf Injectman NI2)(Mello et al ...