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Citations for Eppendorf :
1 - 50 of 90 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... For quantitative real time PCR 1 µl of cDNA was used per reaction with 2x SYBR-Green Master Mix (Peqlab) on a Mastercycler ep realplex S (Eppendorf). UBC (AT5G25760 ...
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bioRxiv - Microbiology 2022Quote: ... Quantitative real-time PCR was carried out using Maxima SYBR Green qPCR Master Mix (2X) (Molecular Biology) and Eppendorf Realplex System (Eppendorf) following the manufacturers’ protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-PCR was performed with EzPCR™ HS Plus 5X PCR master mix (Elpis-Biotech) with a master cycler gradient (Eppendorf®) kit as described above using gene-specific forward primer enolase-F and reverse primer enolase-R ...
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bioRxiv - Microbiology 2021Quote: ... and SYBRGreen PCR Master Mix (Eurogenetec) and data analyzed with the Realplex software (Eppendorf) to determine the cycle threshold (Ct ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplification was performed using EzPCR™ HS Plus 5X PCR master mix (Elpis-Biotech, Daejeon, Korea) with a master cycler gradient (Eppendorf, Hamburg, Germany). PCR conditions were as follows ...
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bioRxiv - Immunology 2021Quote: ... The reaction master mix was added using an X-stream repeating pipette (Eppendorf, Hauppauge, NY) to the microtiter plates ...
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bioRxiv - Immunology 2022Quote: ... The reaction master mix was added using an X-stream repeating pipette (Eppendorf, Hauppauge, NY) to the microtiter plates ...
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bioRxiv - Pathology 2020Quote: ... The reaction master mix were added using an X-stream repeating pipette (Eppendorf, Hauppauge, NY) to the microtiter plates which were covered with optical film (cat ...
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bioRxiv - Immunology 2021Quote: ... The reaction master mix was added to the microtiter plates using an X-stream repeating pipette (Eppendorf, Hauppauge, NY) which were then covered with optical film (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2020Quote: ... Tail biopsies of mice were lysed by a HotShot protocol (incubating the tail sample at 95°C for 30 min in an alkaline lysis reagent followed by neutralization) and used for PCR-based genotyping (GoTaq Green Master Mix, Promega, and Nexus X2, Eppendorf). Perinatal mice were also genotyped fluorescently (BLS miner’s lamp ...
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bioRxiv - Developmental Biology 2023Quote: ... Tail biopsies of mice were lysed by a HotShot protocol (incubating the tail sample at 95°C for 30 min in an alkaline lysis reagent followed by neutralization) and used for PCR-based genotyping (GoTaq Green Master Mix, Promega, and Nexus X2, Eppendorf). Perinatal mice were also genotyped fluorescently (BLS miner’s lamp ...
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bioRxiv - Microbiology 2019Quote: ... similar reaction mixture of PCR except for 1×SYBR Green Real-time PCR Master Mix (Toyobo, Tokyo, Japan) was used and performed at Mastercycler EP realplex2 (Eppendorf, Germany). To estimate the copy numbers of aCPSF1 or RNAP captured genes ...
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bioRxiv - Microbiology 2021Quote: 96 μl of the final OIL-PCR master mix was aliquoted into a 500 μl deep well plate (Eppendorf 00.0 501.101). Nycodenz purified stool cells were diluted in PBS to 104 cells/μl in an 8-well PCR strip for multichannel pipetting ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was run with 2 μl of cDNA and qPCRBIO SyGreen master mix (Cat.# PB20.11-01, PCR Biosystems) on an Eppendorf Mastercycler Ep Realplex (Eppendorf, Hauppauge, NY) using a program of 15 min at 95°C ...
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bioRxiv - Microbiology 2023Quote: ... This PCR master mix was split into triplicates (16.7 μL each reaction) using an epMotion 5075 liquid handling robot (Eppendorf, Hamburg, Germany). Thermocycler conditions were 2 min at 95 °C ...
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bioRxiv - Biophysics 2023Quote: ... 4 µl ATP from the dilution series prepared for the master mix plate (Eppendorf tubes with maximum ATP conc. = 200 µM) were added to row A and row I on reaction stop plate ...
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bioRxiv - Microbiology 2021Quote: ... The qPCR experiment was performed in 96-well PCR plate using Eppendorf Mastercycler® RealPlex with SYBR Green PCR Master Mix (Eppendorf, USA) as per the universal SYBR Green qPCR protocol where fluorescent product is detected during the last step of each cycle ...
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bioRxiv - Plant Biology 2023Quote: ... RT qPCR was performed using 2×Hieff qPCR SYBR Green Master Mix (Yeasen, Shanghai, China) and PCR reactions and fluorescence detection were performed in a Mastercycler ep realplex (Eppendorf, New York, NY). ACTIN 2 (ACT2 ...
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bioRxiv - Plant Biology 2021Quote: ... and VacuTip II microcapillaries (Eppendorf) were used to isolate about 40-50 washed embryos in 50 μl isolation buffer.
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bioRxiv - Cell Biology 2021Quote: ... with Femto tips II (Eppendorf) into the perivitelline space on the ventral side of Stage 10 embryos ...
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bioRxiv - Cell Biology 2022Quote: ... at RT for 2X 5min at 2,000 rpm in a ThermoMixer C (Eppendorf). Liquid was removed ...
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bioRxiv - Microbiology 2022Quote: ... All reactions were carried out in Mastercycler nexus 2x system thermal cycler (Eppendorf). Controls devoid of DNA were simultaneously included in the amplification process ...
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bioRxiv - Molecular Biology 2022Quote: ... The qRT-PCR data was produced by Eppendorf Realplex2 master cycler (Eppendorf AG 223341, Hamburg) and analysis done using delta delta-CT (2□-□ΔΔCT ...
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bioRxiv - Neuroscience 2019Quote: ... 0.3 microliters of 1E12-1E13 gc/mL were injected subretinally in neonatal mouse pups using pulled glass needles controlled using Femtojet injector (Eppendorf).
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bioRxiv - Developmental Biology 2019Quote: ... by using Femtotip II microcapillaries (Eppendorf). Larvae were kept at 18 °C ...
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bioRxiv - Biophysics 2023Quote: ... The injection needles Femtotip II (Eppendorf) were used for injection ...
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bioRxiv - Genetics 2024Quote: ... The injection needle (Femtotips II, Eppendorf) was filled with a mix of 5µg of the plasmid of interest and 1µg of mCherry as an injection control ...
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bioRxiv - Microbiology 2021Quote: ... Amplification was conducted using a master cycler (Eppendorf) programmed with 1 cycle at 95 °C for 1 min ...
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bioRxiv - Physiology 2022Quote: ... on the Master cycler RealPlex4 platform (Eppendorf, Germany). The primer pairs and PCR conditions used are listed in Table 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... and Master Cycler Pro Device (EPPE6324000.516, Eppendorf, DE). For RT-qPCR ...
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bioRxiv - Biophysics 2020Quote: ... mounted to a micromanipulator (Injectman II, Eppendorf).
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bioRxiv - Plant Biology 2023Quote: ... PCRs were performed using the Master Cycler realPlex2 (Eppendorf) in a 96-well reaction plate according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... using Femtotip II microinjection capillaries (Cat#. 5242957.000, Eppendorf) combined with the Eppendorf electronic microinjector FemtoJet 4x (Cat# ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was loaded in Femtotips II (Eppendorf) and injection was done with an injection pressure of 1.0 hPa ...
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bioRxiv - Microbiology 2023Quote: ... with solutions injected through Femtotip II glass microcapillaries (Eppendorf), and the microcapillary position controlled with a MP-285 micromanipulator (Sutter ...
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bioRxiv - Evolutionary Biology 2022Quote: ... sequencing reactions were performed in the Master Cycler pro 384 (Eppendorf) using the ABI BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2023Quote: ... microinjector with Femtotip II microinjection capillary tips (Eppendorf, Cat# 930000043) at 100 hPa pressure for 0.2 seconds ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR analysis was performed on Eppendorf Realplex Master Cycler (Eppendorf, Germany) using KAPASYBRFAST [Mastermix (2× ...
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bioRxiv - Immunology 2019Quote: ... and fluorescence was quantified using an Eppendorf Master Cycler (Eppendorf, Hauppauge, NY). Relative gene expression levels were calculated using the ΔΔCt method (Livak and Schmittgen 2001) ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was performed in a thermocycler (Eppendorf Master cycler X50 Thermocycler) using the following program ...
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bioRxiv - Molecular Biology 2020Quote: ... one milliliter of NS1 recombinant protein (3 mg/ml in 2x PBS) was placed into a 1.5 ml Eppendorf tube (Eppendorf, Germany); and the tube mixed for 66 hours (55°C ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were cleaned up with 2x 100 µl 99% ddH2O 1% TFA into 2x 100 µl 99% Isopropanol 1% TFA at 1200 xg at RT each using a table-top centrifuge (Eppendorf). Afterwards ...
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bioRxiv - Bioengineering 2021Quote: ... with microinjection capillaries (Femtotip II, inner diameter of 500 nm, Eppendorf). For injection ...
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bioRxiv - Microbiology 2021Quote: ... Amplifications were performed in an Eppendorf Master Cycler Model 5345 (Eppendorf, Hamburg, Germany). Each 25 uL reaction contained 12.5 uL 2X reaction mix ...
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bioRxiv - Plant Biology 2021Quote: ... All reactions were performed using a Wagetech Projects Master Cycler (Eppendorf, Hamburg, Germany) with an initial denaturing cycle of 5min at 95 °C ...
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bioRxiv - Biochemistry 2023Quote: ... in 5% glucose solution v/v prior to adding 2X solubilization buffer and incubation at 37 °C for 4 hrs under gentle orbital agitation (500 rpm on Eppendorf Thermomixer). The product was then analyzed by SEC as described earlier.
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bioRxiv - Developmental Biology 2022Quote: ... The mix was injected with a FemtoJet® 4i microinjector (Eppendorf).
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bioRxiv - Microbiology 2019Quote: ... and PCR was conducted using a Master Cycler gradient PCR thermal cycler (Eppendorf, NY). Using GAPDH as the housekeeping gene ...
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bioRxiv - Developmental Biology 2021Quote: Preparation for microinjection was carried out with a Femtotips II microinjection needle (Eppendorf) and a gas pressure injection system were used to inject oil into the Stage 14 egg chambers [31] ...
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bioRxiv - Pathology 2023Quote: ... qRT-PCR was performed using an Eppendorf Realplex2 Master Cycler (Eppendorf AG 223341, Hamburg, Germany). Raw qRT-PCR data were analyzed using the delta delta-CT (2-ΔΔCt ...