Labshake search
Citations for Eppendorf :
401 - 450 of 566 citations for Cell Isolation Optimizing System since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... rinsed by pelleting cells at 5000 rcf for 1 min in a tabletop microcentrifuge (Eppendorf, Hamburg, Germany), and resuspended in carbon-free defined minimal medium such that the final was 1.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... SYBR Green incorporation in cell DNA amplification was determined using the Master epRealplex cycler® (Eppendorf, France) and the following program to increase SYBR Green incorporation ...
-
bioRxiv - Bioengineering 2022Quote: ... Cell- and drug-containing plates were then centrifuged for 5 minutes at 135 x g (Eppendorf 5810R), then incubated for 72 hours at 37°C in 5% CO2 humidified atmosphere with gas permeable plate sealing film (VWR) ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells fell into the cap of a sterile collection tube (0.2 ml PCR tubes, Eppendorf AG, Germany), containing 5 µl of collection buffer (NEBNext Single Cell Lysis Module ...
-
bioRxiv - Cell Biology 2023Quote: ... falciparum 3D7 [129] were cultured in human red blood cells (O+; University Medical Center Hamburg, Eppendorf (UKE)) ...
-
bioRxiv - Microbiology 2023Quote: ... 0.8 mL/well of this cell suspension were then added into Deep Well 96-well plates (Eppendorf) and kept at 37°C ...
-
bioRxiv - Cell Biology 2022Quote: For esiRNA transfection 35,000 U2OS cells were seeded in 2 ml medium in 6-well plates (Eppendorf) the evening before transfection ...
-
bioRxiv - Cell Biology 2022Quote: Cells corresponding to 1 OD600-units were collected in 2.0 ml safe lock tubes (Eppendorf 0030 121.880), washed 1x with H2O and snap frozen in liquid nitrogen ...
-
bioRxiv - Cancer Biology 2023Quote: ... mCherry+ as well as mCherry-cell populations were sorted as bulk populations into 1.5 ml tubes (Eppendorf) and immediately processed for single cell RNA-sequencing using 10X Chromium 3’ sequencing platform.
-
bioRxiv - Cancer Biology 2023Quote: ... MHCI+ or mCherry+ (EGFP+ and EGFP-) cell population was index-sorted into 384 well-plates (Eppendorf Lobind) as single cells ...
-
bioRxiv - Microbiology 2023Quote: ... bacterial cells were removed from 900 μL culture through centrifugation (4 min, 6800 g, Eppendorf™ 5424R). The supernatant (700 μL ...
-
bioRxiv - Microbiology 2023Quote: ... The starting cell concentration was serially diluted in 96-deepwell plates (Deepwell plate 96/2000 μL; Eppendorf), covered with AeraSeal adhesive sealing films (Excel Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... a cell viability assay was performed with serial dilutions in 1.5mL sterile conical tubes (Eppendorf; Hamburg, Germany). Each dilution factor for the respective time points were plated in triplicate on nutrient agar plates by adding 100 µL to the center of the plate and aseptically dispersed using a sterile spreader ...
-
bioRxiv - Cancer Biology 2024Quote: ... Around 5 x 105 or fewer cells were used for staining (96 well plate or Eppendorf tubes). Cells were centrifuged at 1500 rpm for 5 minutes at 4°C ...
-
bioRxiv - Developmental Biology 2020Quote: ... sorted single cells were injected into MII oocytes using a piezo-driven micromanipulator (Eclipse Ti, Nikon; PiezoXpert, Eppendorf). After injection embryos were cultured in M16 medium for 1 hour and subsequently activated for 6 hours in KSOM medium containing 5 mM strontium chloride and 2 mM EGTA ...
-
bioRxiv - Cell Biology 2019Quote: ... The cells were centrifuged down at 13,000 rpm for 30 minutes at 4° C (Eppendorf Centrifuge 5415 R). The supernatant was collected and transferred to a fresh microfuge tube ...
-
bioRxiv - Cancer Biology 2021Quote: ... All cell lines were maintained in 100 mm petri dishes (Corning, Sigma-Aldrich / Nunc, Thermo Fisher Scientific / Eppendorf) at 37°C in a humidified 5% CO2 incubator (Eppendorf) ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were lysed by sonication and centrifuged for 30 min at 4,000 rpm in a 5810R centrifuge (Eppendorf) at 4°C to eliminate cellular debris ...
-
bioRxiv - Bioengineering 2022Quote: ... 50 µL of DNA amplification samples was applied to the flow cells through a micro loader (Eppendorf, USA). The output voltage was proportional to the pH of the DNA samples with a typical sensitivity of 55 mV/pH ...
-
bioRxiv - Cell Biology 2022Quote: U-2 OS cells were grown in 24-well glass bottom plates (170 µm coverglass bottom; Eppendorf #0030741021) coated with poly-l-lysine and treated with 200 µM oleate-BSA complex for 24 hr ...
-
bioRxiv - Microbiology 2020Quote: ... The virus-infected MRC5 cells were collected and centrifuged at 700 × g for 15 minutes (Eppendorf Centrifuge 5810R). Supernatant was filtered by serial filtration steps to eliminate cells and cellular debris ...
-
bioRxiv - Neuroscience 2021Quote: Immunofluorescence microscopy was carried out by seeding out BV2 microglia in a 24-well cell imaging plate (Eppendorf) at a density of 5 × 104 cells/ml ...
-
bioRxiv - Cancer Biology 2022Quote: Cells were seeded on PA hydrogels on round coverslips (18mm diameter) inserted on 24-well plates (0030741021, Eppendorf, black-walled with 170µm coverglass bottom) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... the following steps were performed: After having collected single cells (see 2.4) in single nuclease-free 0.2 mL PCR-tubes (Eppendorf AG ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were pelleted by centrifugation (3220 x g 5 min, Eppendorf 5810R fitted with rotor A-4-81), pellets were recombined with 5 mL pre-chilled MP medium (no carbon) ...
-
bioRxiv - Cell Biology 2022Quote: ... About 5×107 cells as 325 µl suspensions in 1.5 ml safe-lock tubes (Eppendorf AG, Hamburg, Germany) were ruptured by brief pulsed sonication at RT using the ultrasonic homogeniser SONOPULS mini20 ...
-
bioRxiv - Microbiology 2022Quote: ... cells were pelleted by centrifugation for 5 min at 15,700 x g using 5415R Microcentrifuge (Eppendorf, Hamburg, Germany) and the supernatant was frozen at −20° C until further use ...
-
bioRxiv - Microbiology 2022Quote: ... 0.5 mL subsamples of cell cultures normalized as above were dispensed into 2 mL tubes (cat: 0030120094, Eppendorf) and stained with MitoTracker Orange CMTMRos (cat ...
-
bioRxiv - Molecular Biology 2022Quote: ... falciparum 3D7 (68) were cultured in human red blood cells (O+ or B+, Blood bank, Universitätsklinikum Hamburg-Eppendorf). Cultures were maintained at 37°C in an atmosphere of 1% O2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed by shaking at 1,400 rpm for 30 min at 65°C in a Thermomixer (Eppendorf) followed by centrifugation for 5 min at 13,000 g ...
-
bioRxiv - Microbiology 2023Quote: ... we centrifuged 1 mL overnight-cell culture in a 1.5 mL Eppendorf tube at 7’500 rcf for 5 min (Eppendorf, tabletop centrifuge MiniSpin plus with rotor F-45-12-11 ...
-
bioRxiv - Microbiology 2023Quote: ... and the cells were incubated at 37°C/ 5% CO2/ 1% O2 (Galaxy 170 R/ New Brunswick/ Eppendorf). Cells were cultured for up to 14 days with bi-weekly media change ...
-
bioRxiv - Neuroscience 2023Quote: ... The cell culture supernatant was first cleared by centrifugation at 1400 x g for 15 min (5920R; Eppendorf) and then filtered through a 0.45-µm PES filter (Merck Millipore ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... pH 7.6) and injected into 1 to 2-cell-stage embryos by using a microinjector FemtoJet 5247 (Eppendorf). The sequences and the concentrations of MOs (Gene Tools ...
-
bioRxiv - Molecular Biology 2024Quote: A total of 350,000 HEK293-T cells were seeded in each well of 6-well plates (#EP0030720113, Eppendorf). Transfection was performed the day after at 40-50% cell confluence ...
-
bioRxiv - Genomics 2024Quote: ... parvum cells that were isolated using a micromanipulator (MMO-202ND; Narishige) equipped with a microinjector (CellTramm Oil; Eppendorf) that held a capillary (Figure 1A) ...
-
bioRxiv - Cell Biology 2020Quote: ... and cell debris was spun down at 20,000xg for 10 minutes at 4°C using a 5810 R Centrifuge (Eppendorf). Protein concentration was estimated using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... and injected into the nucleus of HeLa or U-2 OS cells using an Eppendorf FemtoJet® Microinjector (Eppendorf). After media were changed ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were sorted using a 100 um nozzle (∼20 PSI) into 2 ml protein LoBind Eppendorf tubes (Eppendorf 0030108132) placed in a cooling holder ...
-
bioRxiv - Bioengineering 2022Quote: ... Cells were harvested by centrifugation (3200 g, 40 min, 4 °C; Eppendorf centrifuge 5810 R, Eppendorf AG, Hamburg, Germany), and the cell pellets were stored (−20 °C) ...
-
bioRxiv - Cell Biology 2020Quote: ... 1% P/S on a magnetic separator cells were cultured in fibronectin-coated 6-well plates (Eppendorf, Hamburg, Germany) in DMEM/F-12 ...
-
bioRxiv - Systems Biology 2021Quote: ... In brief: crude cell lysates were transferred from source plates to 384 well v-bottom PCR Plates (Eppendorf #951020702) assay plates ...
-
bioRxiv - Systems Biology 2021Quote: ... Cell lysates were transferred to a new tube and were centrifuged for 2 min at full speed (Eppendorf centrifuge) and the supernatant was carefully mixed with 1 volume of 70% HPLC-grade ethanol ...
-
bioRxiv - Plant Biology 2021Quote: ... were made from single colonies of putative Xcm strains and cell suspensions (optical density (OD) = 0.3) were generated in MilliQ ultrapure H2O using a spectrophotometer (Eppendorf BioSpectrometer basic ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Cells were then pelleted by centrifugation at 3900 rcf for 15 min (Eppendorf S5810R with S-4-104 rotor) and stored at -80 °C.
-
bioRxiv - Genomics 2019Quote: ... Cells were thawed and pelleted in a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Cell pellets were resuspended in 250 μl nuclei permeabilization buffer (10 mM Tris-HCl pH 7.4 (Sigma) ...
-
bioRxiv - Cancer Biology 2019Quote: ... treated cells washed and scrapped in chilled PBS followed by pop-spin for 10 sec in tabletop centrifuge (Eppendorf). After discarding supernatants ...
-
bioRxiv - Cell Biology 2019Quote: ... Approximately 5– 10 nl of cell suspension was microinjected into the pericardial cavity of the embryo using CellTramVario (Eppendorf), Injectman Ni2 (Eppendorf ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were kept at 37°C and 5% CO2 in a Galaxy 170 R humidified incubator (Eppendorf, Hamburg, Germany). All used cell lines were confirmed to be mycoplasma free by using MycoAlert Mycoplasma Detection Kit (Lonza).
-
bioRxiv - Microbiology 2020Quote: ... were obtained by removing the head of a CO2-anaesthetized adult and drawing 0.5 μL hemolymph from the inner thorax cavity with a Cell Tram Oil microinjector (Eppendorf), using a stereomicroscope ...