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Citations for Eppendorf :
451 - 500 of 566 citations for Cell Isolation Optimizing System since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... cells equivalent to 1 OD600 were pelleted by centrifugation at 2,808 x g for 10 min (Eppendorf 5810 R) and the pellets were flash frozen ...
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bioRxiv - Neuroscience 2020Quote: ... washed twice with 160 µL PBS and nearly 40,000 cells per well transferred onto the membrane and dried with a plate thermomixer (Eppendorf) at 50 °C ...
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bioRxiv - Cell Biology 2021Quote: ... after which the mixture was filtered using a 100 µm cell strainer in to a 1.5 ml tube (Eppendorf). The mixture was then transferred to a clean 1 ml dounce homogenizer to which 750 ul of buffer A containing 1% Igepal (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: Oocyte and 1-cell embryo microinjections were performed using a Femtojet injector and TransferMan 4r micromanipulators (Eppendorf, Enfield, CT) on a Leica DMI 6000B inverted microscope ...
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bioRxiv - Molecular Biology 2022Quote: ... the resulting cell lysates were passed through a 20 G needle 100 times or heated using a Thermomixer (Eppendorf) at 55°C for 10 minutes with 1000 rpm agitation prior to the conventional RNA purification procedure in accordance with the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... supernatant of infected cells was collected and centrifuged for 2.5 min at 25200×g in a refrigerated micro-centrifuge (Eppendorf). Lactate dehydrogenase (LDH ...
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bioRxiv - Cell Biology 2022Quote: Cells were seeded at a density of 0.4 x105 cells onto coverslips (Blue Star) in 24-well petri plates (Eppendorf). After appropriate drug treatment ...
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bioRxiv - Immunology 2023Quote: ... 50 mL of diluted bacterial suspensions were seeded onto a 96-well glass-bottom plates (Cell imaging plate, Eppendorf) and incubated for 30 min at 37°C ...
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bioRxiv - Genomics 2024Quote: ... the 1*105 cells were taken off for ATAC sequencing and placed into DNA LoBind 1.5mL tubes (Eppendorf, 2231000945). The cells were washed 3x in ice cold buffered natrium chloride (PBS ...
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bioRxiv - Immunology 2023Quote: ... Cells were directly FACS sorted without an intermediate MACS column enrichment step using FACSAria Fusion Cell Sorter (Becton Dickinson) in LoBind 1.5 mL microcentrifuge (Eppendorf) tubes with 1:1 FBS:PBS supplemented with recombinant RNase inhibitor (1:100 ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa SS6 or 1.E cells were seeded into 35 mm tissue culture dishes or 6 well tissue culture plates (Eppendorf) with or without pre-cleaned ...
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bioRxiv - Genomics 2021Quote: ... live cell was index-sorted into one 96-well quadrant of a 384-well plate (Eppendorf lo-bind twin-tec) containing a mixture of DPBS and shearing master mix at a final volume of 2.14 μL using a MoFlo Astrios cell sorter running Summit v6.3 (Beckman Coulter) ...
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bioRxiv - Neuroscience 2020Quote: ... 180 μL aliquots of solubilized GlyT1 cell membrane were dispensed in a 4°C-cooled 96-well PCR plate (Eppendorf) in triplicates ...
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bioRxiv - Microbiology 2021Quote: ... Cell harvest was performed outside the anaerobic chamber at 3700 rpm for 12 min (Centrifuge 5920R, S-4×1000, Eppendorf) at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... a 96-well plate pre-filled with 20 μL pure water and sorted with designated cells per well was frozen on dry ice for 5 min and heated by ThermoMixer C (Eppendorf) at 95 °C for 10 min followed by spinning down at 3000 rpm for 1 min ...
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bioRxiv - Microbiology 2021Quote: ... and a volume corresponding to an OD600nm of one (109 cells) was pelleted at 14,000 x g for one minute (Eppendorf MiniSpin). 20 µl of supernatant was mixed with 50 µl BioLux Gaussia Luciferase Assay substrate (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: Single osteoblastic cells were stimulated by local membrane indentation with a glass micropipette using a FemtoJet microinjector NI2 (Eppendorf Inc.), as previously described (Mikolajewicz ...
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bioRxiv - Neuroscience 2020Quote: ... Live tissue slices were mounted onto the coverglass bottom surface of 35mm cell imaging dishes (Eppendorf, 145 μm thickness, 0030740009) coated with Cell-Tak Cell and Tissue Adhesive (Corning™ ...
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bioRxiv - Cell Biology 2021Quote: ... ATP and ADP levels were normalized for an optical density at 600 nm of the initial cell suspension assessed by BioSpectrometer (Eppendorf).
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bioRxiv - Microbiology 2022Quote: ... trypsin was inactivated with 1:1 complete media and the cell suspension was collected into a 5mL protein-low bind centrifuge tube (Eppendorf). To remove residual trypsin ...
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bioRxiv - Neuroscience 2022Quote: ... Lysate was spun at 800 g for 10 min (spin 1) to remove cell debris (Eppendorf Centrifuges 5430 with Eppendorf FA-45-48-11 30-spot 45-degree fixed angel rotor) ...
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Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assemblybioRxiv - Cell Biology 2022Quote: ... The cells were kept at 37°C and 5% CO2 in a humidified incubator (Galaxy 170S CO2, Eppendorf, Hamburg, Germany) and regularly passaged at the confluence of 70-80%.
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bioRxiv - Cell Biology 2022Quote: ... The cells were kept at 37 °C and 5% CO2 in a Galaxy 170S CO2 humidified incubator (Eppendorf, Hamburg, Germany) and regularly passaged at the confluence of 70-80%.
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bioRxiv - Developmental Biology 2020Quote: ... or double (for qRT-PCR) blastomere microinjections of 2-cell (E1.5) stage embryos were performed using FemtoJet or FemtoJet 4i (Eppendorf; 5252000013) micro-injectors ...
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bioRxiv - Neuroscience 2022Quote: ... Cells from each brain region were sorted in a separate A1 well of a 96 well PCR plate (Eppendorf, 951020401) containing 100 µl of PBS buffer with 0.3% BSA ...
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bioRxiv - Neuroscience 2022Quote: ... cell pellets were resuspended in 1mL of ice-cold PBS and transferred to 2 mL LoBind Eppendorf tubes (Eppendorf #022431102). Cells were centrifuged for 2 minutes at 800rpm before being flash-frozen on dry ice ...
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bioRxiv - Biochemistry 2019Quote: ... Varying volumes of viral supernatant were mixed with 5 × 106 to 1 × 107 log phase Jurkat cells in a final volume of 10 ml within a T-25 flask (Eppendorf) and subsequently cultured for 72 hours ...
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bioRxiv - Microbiology 2019Quote: ... C6/36 were used to produce previous ZIKV and DENV viral stocks with high titers in 150-175 cm2 cell culture flasks (Eppendorf), and incubated at 33°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2020Quote: The cell cultures were then transferred into 2 ml reaction tubes and placed in slots in a table-top thermomixer (Eppendorf) set to 37° C and shaking at 700 rpm for five minutes prior to pulse-labelling ...
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bioRxiv - Microbiology 2021Quote: ... Freshly laid eggs were collected from breeding tanks and the solution was injected in the yolk sac of the egg before the emergence of the first cell with a FemtoJet 4i (Eppendorf).
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bioRxiv - Plant Biology 2019Quote: ... The vectors were used to transform Agrobacterium tumefaciens GV3101 cells (carrying the helper plasmid pSOUP) via electroporation at 1800 V using the Electroporator 2510 (Eppendorf). Agrobacteria were screened via colony PCR using primers flanking the 35S promoter region and the terminator region of the T-DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... albeit the injected droplets were collected in fractions of ∼1000 cells (∼27 µl of loaded droplets) in 1 ml LoBind tubes (Eppendorf) pre-filled with 200 µl of mineral oil ...
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bioRxiv - Microbiology 2020Quote: ... Newly emerged EVV-1 free EvaBx adults were CO2 anaesthetized and injected with 0.5 μL of the virus-containing extract between two abdominal segments using a stereomicroscope with a Cell Tram Oil microinjector (Eppendorf). As negative control in order to exclude any procedural contamination ...
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bioRxiv - Genomics 2019Quote: ... Deep red-) Single cells were sorted into 5 ul of RLT 1%β-Mercaptoethanol in a 96 well plate (Eppendorf) and frozen at −80°C.
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bioRxiv - Biochemistry 2021Quote: ... CD34-) (Supplementary Fig. 1) were sorted at single-cell resolution into a 384-well Eppendorf LoBind PCR plate (Eppendorf AG) containing 1 μl of lysis buffer (50 mM Triethylammonium bicarbonate (TEAB ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM β-glycerol phosphate and 2.5 mM Na2H2P2O7).) and cells scraped and transferred into Protein Lo-bind tubes (Eppendorf). Samples were sonicated for 10 cycles (30s on & 40s off ...
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bioRxiv - Immunology 2020Quote: ... The isolated CD8+ T cells were then centrifuged at 400xg for 10 min (Eppendorf Centrifuge 5810R, rotor A-4-62) at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Freshly laid eggs were collected from breeding tanks and the solution was injected in the yolk sac of the egg before the emergence of the first cell with a FemtoJet 4i (Eppendorf).
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bioRxiv - Microbiology 2020Quote: ... We collected individual consortia of host-epibiont cells using an Eppendorf PatchMan NP2 micromanipulator equipped with 6 μm-diameter microcapillaries (Eppendorf) mounted on a Leica Dlll3000 B inverted microscope ...
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bioRxiv - Microbiology 2020Quote: Newly-emerged adults were anaesthetized with CO2 and microinjected between two abdominal segments under a stereomicroscope using a fine glass needle connected to a Cell Tram Oil microinjector (Eppendorf). Insects were microinjected with 0.5 μL of dsRNAs at the concentration of 160 ng μL-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Each sample was processed on an independent Chromium Single Cell A Chip (10X Genomics) and run on a thermocycler (Eppendorf). 5’ gene expression libraries were sequenced using NextSeq 500 150-cycle high-output flow cells or NovaSeq 6000 200 cycle S4 flow cells ...
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bioRxiv - Cell Biology 2022Quote: All cells were kept at 37°C and 5% CO2 in a Galaxy 170 R humidified incubator (Eppendorf, Hamburg, Germany). They have also been regularly tested for mycoplasma contamination by examining the samples for extracellular DNA staining with SiR-DNA (100 nM ...
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bioRxiv - Microbiology 2022Quote: ... The cell suspension was then transferred into 2 mL tubes and centrifuged at 16 000g and 4°C (Eppendorf, Germany) to pellet the cells ...
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bioRxiv - Microbiology 2022Quote: ... Neutrophils were then co-incubated with bacterial strains at an MOI of 1:1 ratio in RPMI 1640 cell culture medium (HIMEDIA) and incubated at 37⁰C in an animal tissue culture incubator (Eppendorf). The same method was performed for macrophage RAW 264.7 cell line in the DMEM medium (HIMEDIA) ...
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bioRxiv - Microbiology 2023Quote: ... 2ml of cells were collected at different time points and was centrifuged at 2,348g (FA-45-24-11, Eppendorf rotor) for 5 minutes at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the growth medium was aspirated and Cultrex droplets were gently dislodged into the cell recovery solution (Cat. No. 354270, Corning and transferred to Eppendorf tubes using wide-bore 1 ml pipette tips ...
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bioRxiv - Genomics 2023Quote: ... gating was established and single cells were sorted (130 micron nozzle assembly) into low-bind 96 well PCR plates (Eppendorf twin.tec LoBind ...
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bioRxiv - Neuroscience 2023Quote: ... The 1 ml of PBS with the resuspended cells was transferred to a 1·5 ml Protein LoBind tube (Eppendorf), centrifuged at 3000 x g for 6 min at 4 °C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We collected these bacteria into KK2 with a sterile loop and diluted them to 1.5 OD600 in KK2 (∼5 × 108 cells, measured with an Eppendorf BioPhotometer). We used these bacteria as food for amoebas in our experiment and repeated this process anew for each of the three replicate experiments.
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bioRxiv - Genetics 2023Quote: Zebrafish embryos were collected and injected as previously described (Rosen et al., 2009) at one-cell stage using a FemtoJet Injector (Eppendorf) or PV820 injector (WPI ...