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Citations for Eppendorf :
401 - 450 of 1285 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... To create extrachromosomal arrays constructs were microinjected into distal gonads of young adults using inverted microscope Leica DMi8 equipped with DIC filters and microinjection system InjectMan® 4 and FemtoJet® 4i (Eppendorf). Microinjection mixtures contained 10 ng/μL of the plasmid of interest ...
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bioRxiv - Developmental Biology 2024Quote: Chromatin was pre-cleared by centrifugation at 14,000×g for 15min at 4°C and the supernatant was moved to a 1.5mL DNA LoBind® tube (Eppendorf: Hamburg, Germany). From each chromatin sample per phenotype per lineage ...
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bioRxiv - Bioengineering 2024Quote: ... 500μl of samples previously diluted by 10 in DPBS were deposited in Amicon® filter before centrifugation at 2000g during 15min at 4°C (Centrifuge 5804 R, Eppendorf®). Then ...
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bioRxiv - Biochemistry 2022Quote: ... and samples were vortexed at 1200 rpm for 2-3 hours at room temperature (MixMate ®, Eppendorf South Pacific, Sydney, NSW Australia). Following this ...
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bioRxiv - Microbiology 2021Quote: ... The media was spun down in a centrifuge at 4°C for 10 min at 4400 g (Centrifuge 5920 R, Eppendorf Nordic, Hørsholm, Denmark) and the supernatant collected and stored at -80° for 1H NMR analysis ...
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bioRxiv - Microbiology 2021Quote: ... Development of ∼60 % cytopathic effect (CPE) led to harvesting and clarification of supernatants (3184 x g, 20 min, 4 °C, in an Eppendorf 5810 R centrifuge). Aliquots were then titred by plaque assay (see below) ...
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bioRxiv - Microbiology 2021Quote: The rest of the supernatant cell suspension (~19 ml) was centrifuged in a swing-out rotor (Eppendorf A-4-62 Swing Bucket Rotor) at 3200 × g for 10 min to pellet cells ...
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bioRxiv - Neuroscience 2023Quote: ... blood samples were allowed to clot at room temperature for 30 minutes before undergoing centrifugation at 1500 × g for 10 minutes at 4°C using a refrigerated centrifuge (Eppendorf AG, Hamburg, Germany). The supernatant serum was pipetted into sterile 1.5 mL Eppendorf tubes and stored at -80°C until further analysis.
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bioRxiv - Cell Biology 2024Quote: The cilia suspension was thawed on ice and then centrifuged at 16000 g and 4°C for 10 minutes in a microfuge in a refrigerated room (Eppendorf, Centrifuge 5415 D). The pellet was resuspended in 250 μl of ice-cold Cilia Final Buffer (50 mM HEPES at pH 7.4 ...
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bioRxiv - Physiology 2024Quote: ... Blood was allowed to clot on ice for 20 minutes then was spun down in a cold centrifuge (4°C, Eppendorf microcentrifuge, model 5415R) for 20 minutes at 2000 g ...
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bioRxiv - Cell Biology 2020Quote: Germinal vesicle intact oocytes were microinjected with ~5 pL of cRNAs in M2 medium containing milrinone at room temperature with a micromanipulator TransferMan NK 2 (Eppendorf) and picoinjector (Medical Systems Corp.) ...
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bioRxiv - Genomics 2024Quote: ... 1 mL of well-grown culture was centrifuged (5 min, 2040 g; Eppendorf) and the superfluous medium was removed by pipetting ...
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bioRxiv - Microbiology 2022Quote: ... incubated (1 h) and centrifuged (Eppendorf centrifuge R5810, 4000 rpm for 5 min) for β-galactosidase activity quantification ...
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bioRxiv - Genomics 2020Quote: The ST slide with the tissue section was warmed to 37°C for 4 minutes on a thermal incubator (Eppendorf Thermomixer Option C, Germany) and in situ fixed and washed as described above ...
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bioRxiv - Cell Biology 2020Quote: ... This isolation method included a penultimate centrifugation step in Eppendorf polypropylene conical tubes (10,000 x g for 30 min at 4°C, in Eppendorf rotor F-34-6-38) that allowed the removal/isolation of larger microvesicles ...
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bioRxiv - Microbiology 2020Quote: ... The presumptive cultures were grown in TSBYE broth for 24 h at 30°C and centrifuged at 4°C (Eppendorf, 5810R, Auckland, New Zealand) at 3,220 × g for 10 min ...
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bioRxiv - Biochemistry 2023Quote: ... 12,000 rpm was applied for 30 minutes at 4 °C to obtain the supernatant and then evaporated to obtain a dry metabolite pellet (Eppendorf Concentrator plus, Hamburg, Germany). For the NMR experiments ...
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bioRxiv - Plant Biology 2022Quote: ... with incubation on ice for 1 h followed by centrifugation at 4 °C for 20 min at 20,000 g using an Eppendorf Centrifuge 5430R microcentrifuge (Eppendorf Canada Ltd, Mississauga, ON, Canada), supernatant combined and either used same day or stored at –80 °C.
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bioRxiv - Biochemistry 2024Quote: ... Proteins were pelleted by centrifugation at 15.000 x g (10 min, 4°C in cold cabinet, Eppendorf centrifuge #5418, rotor FA-45-18-11). After allowing the pellet to air dry ...
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bioRxiv - Microbiology 2024Quote: ... The NPA positive was diluted in 2 ml of MEM without FBS and centrifuged at 200Xg for 5 min (HSR Centrifuge Eppendorf Presvac) (NPA supernatant) ...
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bioRxiv - Neuroscience 2024Quote: ... The adjacent 3-5 sections of 100 micron tissue sections were collected in a pre-chilled 2mL microcentrifuge tube (Eppendorf Protein LoBind Tube, Cat #22431102) to total a volume of approximately 40 mg for each donor ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with 500μl of D-PBS and centrifuged at 400xg for 10 minutes at room temperature (Eppendorf Centrifuge 5810R, rotor A-4-62). The supernatant was removed and the cell pellet resuspended in 50μl of D-PBS before being stained with CD20-PE (clone A1SB12 ...
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bioRxiv - Neuroscience 2021Quote: ... were incubated with ten-fold molar excess S-XL6 for 4 hours at 37 °C in protein low bind Eppendorf tubes using Eppendorf Thermomixer at 350 rpm (Eppendorf North America, Enfield, CT, USA). After incubation ...
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bioRxiv - Bioengineering 2023Quote: ... MPCs were washed twice for 3 min at 400 RCF at room temperature with PBS supplemented with 1% glucose and 1% pen-strep (Eppendorf 5702R, Hamburg, Germany). Biotinylation of the cell surface was performed by adding 1 mM Sulfo-NHS-LC-Biotin (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... The 1 ml of PBS with the resuspended cells was transferred to a 1·5 ml Protein LoBind tube (Eppendorf), centrifuged at 3000 x g for 6 min at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... 3-liter Bioflo 110 (Eppendorf) were used ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were diluted to 2 x 107 cells per mL and 50 µL of each sample was added to 5 mL tubes (Eppendorf, Hamburg, DE), to constitute 1 million cells per flow sample.
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bioRxiv - Biochemistry 2020Quote: Microdissected tissue samples were lysed in SDT buffer (4% SDS, 0.1M DTT, 0.1M Tris/HCl, pH 7.6) in a thermomixer (Eppendorf ThermoMixer® C, 30 min, 95°C, 750 rpm). After that ...
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bioRxiv - Microbiology 2022Quote: ... Trophozoites were harvested from their growth support by incubating the tubes by tapping the glass tubes followed by centrifugation (Eppendorf centrifuge 5810R, rotor A-4-62) according to a previously reported protocol 84.
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bioRxiv - Bioengineering 2024Quote: ... Cultures were further incubated overnight (900 RPM, 20 °C, 70 % humidity, 20 h) and harvested by centrifugation (Eppendorf 5810R; 3,220 x g, 4 °C, 15 min). The obtained cell pellet was resuspended with 150 µL buffer (50 mM Bicine ...
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bioRxiv - Microbiology 2022Quote: ... and incubated for 1 h before centrifugation (Eppendorf centrifuge R5810, 4000 rpm for 5 min), followed by quantification of β-galactosidase activity.
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei were collected in pre-chilled 1% BSA-blocked 5 mL Protein LoBind tube (Eppendorf) containing the 2% BSA-PBS wash buffer detailed above and kept on ice.
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bioRxiv - Biophysics 2020Quote: ... Supplementary Table 5) were incubated together for 2 h at 37 °C under gentle shaking (450 rpm, Eppendorf ThermoMixer® C, Eppendorf AG, Germany). Unbound DNA origami was removed by placing the tube on a magnet and discarding the supernatant ...
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bioRxiv - Biochemistry 2021Quote: ... Unreacted dye was removed by two passages over an equilibrated Bio-spin® 6 column filled with Bio-gel P-6 media in labeling buffer and centrifuged in a tabletop centrifuge (Eppendorf 5810 R, rotor A-4-62) at 1500 rpm for 3 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The mixture was sonicated 3 times for 1 minute in a waterbath sonicator and incubated in a ThermoMixer (Eppendorf) for 30 minutes at 37°C and 500 rpm ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the lung homogenate was added to 2 ml DNA LoBind tubes (Eppendorf) alongside 500 µl sterile killing buffer (20 mM Tris-HCl pH 7.5 ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Cells (2 OD600) were harvested at 600 x g for 1 min (MiniSpin Centrifuge, Eppendorf) and the supernatants were discarded ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmid DNA (1-5 μg/μl) was microinjected into the fourth ventricle of the embryos (FemtoJet; Eppendorf). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... The 15 mL polypropylene tubes were replaced with 1% BSA-blocked 5 mL Protein LoBind tubes (Eppendorf). Briefly ...
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bioRxiv - Biophysics 2022Quote: ... the DNA droplet-containing aqueous phase was layered on top of the oil phase in a volumetric ratio of 1:3 within a microtube (Eppendorf). For the segregation of DNA droplets using enzymatic activity ...
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bioRxiv - Biophysics 2022Quote: ... The aqueous solution was layered on top of the oil-surfactant mix in a volumetric ratio of 1:3 inside a microtube (Eppendorf). The tube was manually shaken for about 30 s until water-in-oil droplets formed ...
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bioRxiv - Biophysics 2020Quote: ... the DNA-containing aqueous phase was layered on top of the oil phase in a volumetric ratio of 1:3 within a microtube (Eppendorf). Droplet formation was induced by manual shaking for about 4 s as described earlier.[26] For the oil-phase ...
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bioRxiv - Genomics 2024Quote: ... and washed in nuclease-free H2O before proceeding to MNase digestion with 1-2U of MNase for 3×106 cells that went on for 1-hour at 37C while shaking in a thermomixer (Eppendorf) at 550rcf ...
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bioRxiv - Cell Biology 2020Quote: ... and 5 % CO2 (Eppendorf). Jurkat CD4-KO cells were derived from wild-type Jurkat T cells (clone E6 ...
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bioRxiv - Biophysics 2020Quote: 5) Centrifuge (Eppendorf, 5810R)
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bioRxiv - Neuroscience 2020Quote: ... centrifuged (16000 g, 3 min, 5415R, Eppendorf) and re-suspended in fresh medium ...
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bioRxiv - Microbiology 2020Quote: ... nine 1 mL aliquots were centrifuged for 2 min at 2500 rcf (Eppendorf, Centrifuge 5424 R) and the MOPS-based supernatant removed ...
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bioRxiv - Biophysics 2020Quote: ... with three ssDNA overhang strands on a bottom partially complementary to the sequence on the magnetic beads (mag2, Supplementary Table 5) were incubated together for 2 h at 37 °C under gentle shaking (450 rpm, Eppendorf ThermoMixer® C, Eppendorf AG, Germany). Unbound DNA origami was removed by placing the tube on a magnet and discarding the supernatant ...
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bioRxiv - Microbiology 2023Quote: ... we centrifuged 1 mL overnight-cell culture in a 1.5 mL Eppendorf tube at 7’500 rcf for 5 min (Eppendorf, tabletop centrifuge MiniSpin plus with rotor F-45-12-11 ...
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bioRxiv - Neuroscience 2023Quote: ... 5 % CO2 using microloaders (Eppendorf). Slices were placed into the incubation chambers one at a time (minimum volume 1.5 ml to cover raised grid) ...