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Citations for Eppendorf :
301 - 350 of 1285 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... the bacterial suspension was subjected to centrifugation at 4000 rpm at 4 °C for 15 min (5804R, Eppendorf, Germany). The resulting pellet was rinsed with sterile distilled H2O and then reconstituted in an equivalent volume of sterile distilled H2O ...
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bioRxiv - Microbiology 2024Quote: ... Debris was removed from sonicated lysates by centrifugation for 60 minutes at 4 °C and 14,000 × g in a 5910 R centrifuge (Eppendorf). The soluble lysate was then decanted and protein was purified using immobilized metal affinity chromatography ...
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bioRxiv - Microbiology 2024Quote: ... followed by centrifugation at 21,130 x g at 4°C for 10 min in a tabletop chilled centrifuge (Eppendorf). 250 μl of the clarified lysate was transferred to a pre-chilled tube and mixed with 750 μl of TRIZOL LS (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... The resulting parasite solution was pelleted at 1,000 x g at 4°C for 8 min using a chilled large tabletop centrifuge (Eppendorf). The pellet obtained from two D150 plates underwent three washes with intracellular buffer before being resuspended in 200 μl of ice-cold polysome lysis buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... The parasite solution was pelleted at 1,200 x g at 4°C for 10 min using a chilled large tabletop centrifuge (Eppendorf). The resulting pellet underwent 3 washes with intracellular buffer ...
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bioRxiv - Physiology 2024Quote: ... plasma samples were thawed from storage at -80°C and centrifuged (5,000 x g, 10 minutes, 4 °C; Eppendorf 5427R with rotor FA-45-48-11 ...
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bioRxiv - Biochemistry 2024Quote: ... 200 µL of K562 cell lysates (2.5 mg/mL) was added to the beads and incubated at 4 °C on thermomixer (5382000023, Eppendorf) at 1,000 rpm for 2 hours ...
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bioRxiv - Zoology 2024Quote: ... kept overnight at -20°C then centrifuged at 12000 rpm for 15 mins at 4°C (Eppendorf 5810R, USA). The precipitate was allowed to be air-dried and dissolved in 1 ml of 1M Tris-HCl buffer (pH 6.8) ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were pelleted by centrifugation at 4,500xg for 10 min at 4 °C (High-Speed Centrifuge CR22N, Eppendorf). The supernatant was collected and doubly filtered (with a glass filter then with a PVDF filter) ...
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bioRxiv - Biophysics 2024Quote: ... the protein was concentrated to 250μl using a 100kDa molecular weight cut-off filter (Amicon), centrifuged to pellet aggregates (10,000g, 10min, 4°C) (5418R, Eppendorf) and injected in a Superdex 200 10/300 gel filtration column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... heat- denatured FCS matrix was a supernatant obtained after spinning (15,000 g, 15 minutes, 4 °C) 100% FCS (200 uL; 1.7 mL Eppendorf tubes) heated at 85°C for 15 minutes in a Thermomixer Comfort heating block (Eppendorf ...
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bioRxiv - Genetics 2024Quote: ... equipped with a micromanipulator (MMO-220A and MN-4; Narishige, Tokyo, Japan) coupled to a FemtoJet express system (Eppendorf) with the following settings ...
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bioRxiv - Cell Biology 2021Quote: ... cells were recovered in 1 mL YPD (1% yeast extract, 2% Bacto peptone, 2% D-glucose) at 30°C in a ThermoMixer C (Eppendorf, Hamburg, Germany) for 3 hours (200 RPM ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM label was subsequently added and samples were incubated over night at 4°C and 350 rpm in a Thermomixer (Eppendorf). Excess label was removed by using a Sephadex G25 column (GE Healthcare Bio-Science AB ...
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bioRxiv - Bioengineering 2022Quote: ... The lysates were clarified by centrifugation at 18,500g at 4 °C for 30 minutes using tabletop centrifuge (Eppendorf Centrifuge 5810R). Soluble protein was separated from cell debris and the supernatant was passed through 5 mL HisTrap columns (Cytiva ...
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bioRxiv - Neuroscience 2021Quote: ... Single EGFP+ cells were sorted on a BD Influx equipped with a 140 µm nozzle and a cooling unit with a sample temperature of 4°C and collected into a DNA LoBind tube (Eppendorf) containing aCSF with 0.5% BSA ...
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bioRxiv - Genetics 2020Quote: ... The supernatant solutions were centrifuged at 15,800xg for 15 min at 4°C in a microcentrifuge (Model 5042, Eppendorf, Inc.) and the resultant supernatant fractions were stored at −80°C ...
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bioRxiv - Immunology 2021Quote: Xela DS2 (passages 120 – 130) and Xela VS2 (passages 125 – 135) (n = 4 independent trials) were seeded in a 6-well plate (Eppendorf) at a cell density of 625,000 cells/well in 1 mL of Xela complete media and allowed to adhere overnight at 26 °C ...
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bioRxiv - Neuroscience 2020Quote: ... 180 μL aliquots of solubilized GlyT1 cell membrane were dispensed in a 4°C-cooled 96-well PCR plate (Eppendorf) in triplicates ...
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bioRxiv - Microbiology 2021Quote: ... Cell harvest was performed outside the anaerobic chamber at 3700 rpm for 12 min (Centrifuge 5920R, S-4×1000, Eppendorf) at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... the cells were transferred into the anaerobic chamber and harvested for 12 min at 25°C and 3700 rpm (Centrifuge 5920 R, S-4×1000, Eppendorf) outside of the anaerobic chamber ...
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bioRxiv - Biochemistry 2021Quote: ... The reactions were stopped by the addition of phosphoric acid and 4 µl of each reaction were spotted on P81 phosphocellulose papers (Whatman) using the epMotion 5070 (Eppendorf) workstation ...
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bioRxiv - Cell Biology 2021Quote: ... The resuspended cilia sample was centrifuged at 16,000g for 10 min at 4°C in a microfuge (Eppendorf, Centrifuge 5415D). The supernatant was discarded ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plasma was defrosted and then centrifuged at 2000 rpm (805 × g) for 4 min in a 5810 R centrifuge (Eppendorf) to remove possible particulate matter ...
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bioRxiv - Immunology 2020Quote: ... The cultures were placed on ice for 10 minutes to slow growth then centrifuged at 3,000 × g for 10 minutes at 4°C (Eppendorf 5810R). The supernatants were discarded and the pellets were resuspended in fresh RPMI 10mM glucose until a McFarland of 5.0 (∼109 CFU/mL ...
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bioRxiv - Systems Biology 2020Quote: ... A fixed volume of 100 μL cells was used to inoculate 50 mL preculture M9 +40 mM MOPS media with 4 g/L glucose which was grown overnight in a shake flask at 200 rpm in a 37°C (Eppendorf) incubator ...
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bioRxiv - Immunology 2020Quote: ... The isolated CD8+ T cells were then centrifuged at 400xg for 10 min (Eppendorf Centrifuge 5810R, rotor A-4-62) at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Peptides were eluted in 20 μL of 80% acetonitrile in 0.1% TFA and were concentrated to 4 μL (Concentrator 5301, Eppendorf AG). The peptides sample was then diluted to 5 μL by 0.1% TFA for LC-MS/MS analysis.
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bioRxiv - Neuroscience 2023Quote: ... Homogenates were centrifuged at 16,100 g for 20 min at 4 °C with an Eppendorf 5415R centrifuge (Eppendorf, Hamburg, Germany), sonicated for 10 s and protein amounts normalized following a bicinchoninic acid protein assay (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: ... samples were layered on top of the 105 µl cushion and spun at 10,000 g for 20 min at 4°C in a swing out rotor (A-8-11 swing bucket rotor, Eppendorf) to isolate CpxII bound to trans SNARE complexes (SNAREpins ...
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bioRxiv - Molecular Biology 2024Quote: ... for 30 min-4 h at 76°C in a heat block (speed of 300 rpm; ThermoMixer F1.5, Eppendorf, Germany) (Wagner et al. ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then pelleted by centrifugation at 3900 × g for 20 min (5810R centrifuge with S-4-104 rotor, Eppendorf) and stored at −20 °C.
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bioRxiv - Synthetic Biology 2024Quote: ... + 90% air or 10% 12CO2 + 90% air generated by a DASGIP MX4/4 stand-alone gas-mixing module (Eppendorf, Germany). The flasks were placed in a 37°C shaker incubator ...
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bioRxiv - Developmental Biology 2024Quote: ... and cells were sorted using a BD FACS Aria cell sorter at 4°C and collected in 0.4 mg/mL BSA in DNA LowBind tubes (Eppendorf, 0030108523). Cells were pelleted at 300 g for 5 minutes at 4°C and manually counted using a Bürker Chamber ...
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bioRxiv - Neuroscience 2023Quote: ... The tubes were centrifuged at 3000 x g for 6 min at 4 °C and the conditioned media was transferred to a Protein LoBind tube (Eppendorf) (media fraction) ...
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bioRxiv - Microbiology 2023Quote: ... The sample was vortexed for 30 seconds before being centrifuged for 15 mins at 4°C at 1739 x g (3000 rpm, Eppendorf 5810R benchtop centrifuge ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture was then incubated at 37 °C for 4 hrs under gentle orbital agitation (300 rpm on Eppendorf Thermomixer) and centrifuged at 15 000 × g for 5 min prior to SEC analysis ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... the samples were 10000xg centrifuged for 10 min in a cooled centrifuge (4°C; Centrifuge 5415 R, Eppendorf, Hamburg, Germany), and the supernatant transferred to new microcentrifuge tubes ...
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bioRxiv - Molecular Biology 2023Quote: ... After centrifugation for 10 min at 15,000 x g and 4 °C with a 5415R benchtop microcentrifuge (Eppendorf, Hamburg, Germany), the supernatants were collected and residual sample pellets were reextracted with a 200 μL aliquot of the previously used extraction solvent mixture ...
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bioRxiv - Cell Biology 2023Quote: ... followed by ϕ29 polymerase heat inactivation at 65°C for 20 min and cooling at 4°C using PCR thermocycler (Eppendorf). Amplified C-circle DNA was diluted with 2X SSC buffer and loaded onto a dot-blot apparatus bedded with Hybond N+ membrane (RPN2020B ...
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bioRxiv - Cell Biology 2024Quote: ... the cell lysate was collected and agitated for 30 min at 800 rpm at 4 °C in a Thermomixer (Eppendorf). Afterwards ...
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bioRxiv - Systems Biology 2024Quote: ... Samples were transferred to SMART Digest Trypsin digest resin in PCR strips and incubated 4 h at 70°C and 1400 rpm mixing (Thermomixer C with ThermoTop, Eppendorf). Cysteines were 1 ...
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bioRxiv - Microbiology 2024Quote: ... was mixed with an equal volume of acidic phenol pH 4.3 and centrifugated (14,000 rpm for 20 minutes at 4°C in 5418 R Centrifuge equipped with FA-45-18-11 rotor (Eppendorf)) ...
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bioRxiv - Microbiology 2024Quote: ... After centrifugation (14,000 rpm for 20 min at 4°C in 5418 R Centrifuge equipped with FA-45-18-11 rotor (Eppendorf)) ...
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bioRxiv - Neuroscience 2024Quote: ... The plates were centrifuged at 2,000 × g for 20 minutes at 4°C using a swinging-bucket microplate adaptor (Eppendorf, Germany). After adding 180ul or 450 µl of mitochondrial assay buffer ...
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bioRxiv - Microbiology 2024Quote: ... Biomass samples were collected in mid-exponential phase by centrifuging (3214 x g; Eppendorf 5810 R, S-4-104 rotor) 50 mL of culture for 15 minutes at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... which were centrifuged at 3220 x g for 30 min at room temperature (Eppendorf A-4-62 Swing Bucket Rotor). The interface layer containing microbial cells was recovered ...
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bioRxiv - Cancer Biology 2024Quote: ... extracts were centrifuged for 20 minutes at 18,000 x g (4°C) using a Eppendorf™ Centrifuge 5430 R (Eppendorf), to pellet out cellular debris ...
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bioRxiv - Microbiology 2021Quote: ... pelleted by centrifugation (300 rcf, 3 min, RT in 50-mL conical tubes; then 21,000 rcf, 2 min, RT in Eppendorf tubes), and stored at -80 °C ...
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bioRxiv - Microbiology 2021Quote: ... pelleted by centrifugation (300 rcf, 3 min, RT in 15-mL conical tubes; then 21,000 rcf, 2 min, RT in Eppendorf tubes), and stored at -80 °C until further analysis ...