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Citations for Eppendorf :
201 - 250 of 753 citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... Samples were thawed shortly before mass spectrometric analysis and shaken for 2 minutes at 2000rpm (thermomixer C, Eppendorf). Peptide concentrations were measured optically at 280nm (Nanodrop 2000 ...
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bioRxiv - Immunology 2020Quote: ... 10 g NaCl) grown in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf). Cells were grown at 37°C to an OD600 ~0.8 ...
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bioRxiv - Cell Biology 2022Quote: ... the pellet was hydrolyzed in 3N HCl for 2 hours at 95 °C on a shaking Thermomixer (Eppendorf). The reaction was quenched with 100 % methanol containing 40 μM L-norvaline (as an internal control) ...
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bioRxiv - Paleontology 2020Quote: ... 5 mg of bone powder was transferred to a 2 mL Eppendorf® tube (Eppendorf; Westbury, NY, USA) and 1 mL of demineralizing solution (5% trifluoroacetic acid (TFA ...
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bioRxiv - Biochemistry 2022Quote: ... The collected powder was homogenised and stored in 2 mL protein LoBind tubes (Eppendorf UK Limited, Stevenage, UK) at −80°C until extraction and testing ...
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bioRxiv - Neuroscience 2022Quote: ... 1-2 fecal pellets were collected from each mouse by voluntary defecation into a sterile microtube (Eppendorf, Germany) and fecal samples were immediately placed on dry ice ...
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bioRxiv - Microbiology 2023Quote: ... two milliliters of the fermentation broth containing the suspended mucin beads were sampled to 2-ml tubes (Eppendorf). The serum bottles were opened and closed carefully each time to avoid contamination in the anaerobic workstation ...
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bioRxiv - Cancer Biology 2023Quote: ... and then gently shaken at 300 RPM for 2 hr at 37°C using a Thermomixer C (Eppendorf). Sample pH was then adjusted to 4 with 15 μL of 20% formic acid before loading onto conditioned SPE Bond-Elute pH columns (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... 2 μM final test concentration) were mixed (reaction mix) and transferred into a white 96 well plate (Eppendorf Twin-Tec ...
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bioRxiv - Cell Biology 2024Quote: ... 2 dpf zebrafish larvae close to the heart by using an electronically regulated air-pressure microinjector (FemtoJet, Eppendorf). After injection ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were plated on 24-well cell imaging plates (black plate with treated glass bottom, Eppendorf) and treated with siRNAs and 100nM nocodazole accordingly ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were plated on 24-well cell imaging plates (black plate with treated glass bottom, Eppendorf) and treated with drugs and/or siRNAs accordingly ...
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bioRxiv - Developmental Biology 2021Quote: ... cell imaging dish Catalog #0030740009 (Eppendorf) and submerged in imaging solution (80% egg white and 20% 1.2% UltraPure LMP Agarose Catalog #16500100 (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... cells were harvested by centrifugation (Eppendorf) at 4°C for 10 min at 7000 g and then washed twice with and re-suspended in the relevant suspending liquid (NaCl 150mM or 1.5 mM) ...
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bioRxiv - Microbiology 2023Quote: ... on Cell Imaging Plates (Eppendorf, Germany). Immediately before the infection or 1 ...
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bioRxiv - Neuroscience 2021Quote: ... The extraction step incorporated a 2-fold concentration of saliva samples using a vacuum concentrator (Concentrator plus, Eppendorf, Germany) resulting in a detection sensitivity of 3pg/ml ...
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bioRxiv - Genomics 2020Quote: ... cultures were diluted to OD ∼ 0.02 in 1.4 mL of LB and were further grown for 2 h at 37°C on a Thermomixer (Eppendorf) with shaking (700 rpm) ...
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bioRxiv - Biochemistry 2021Quote: ... β-chitin aliquots from the culture flasks were transferred to 2 mL Safe-Lock Eppendorf tubes (Eppendorf, Hamburg, Germany) and boiled directly for 5 min in 30 µL NuPAGE LDS sample buffer and NuPAGE sample reducing agent (Invitrogen™ ...
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bioRxiv - Immunology 2022Quote: ... incubated with each antigen at certain concentration in PBS/2% FCS and transfer to a 37°C thermomixer (Eppendorf). Cells were collected after each period of incubation and fixed in IC fixation buffer on ice for 30 mins and at RT for additional 30 mins ...
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bioRxiv - Plant Biology 2019Quote: ... elution was performed with 30 μl of 1M Glycine (pH 2.0) buffer for 2 min in a Thermomixer (Eppendorf) at 1200 rpm ...
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bioRxiv - Evolutionary Biology 2020Quote: GV oocytes were microinjected with ~5 pl of cRNAs in M2 medium (with 2.5 mM milrinone and 3mg/mL BSA) at room temperature (RT) with a micromanipulator TransferMan NK 2 (Eppendorf) and picoinjector (Medical Systems Corp.) ...
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bioRxiv - Cell Biology 2020Quote: ... The homogenate was further incubated on ice for 45 min before centrifugation at 21,000 g and 4°C for 2 x 10 min in an Eppendorf 5424 R benchtop centrifuge (Eppendorf). Protein content of the lysate was determined by BCA assay (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... we further purified the extracts by treatment with 1% sodium dodecyl sulfate in NaP-EDTA buffer and centrifugation at 13,000 rpm for 10 min (rotor FA-24×2, Eppendorf). This purification process was repeated two times ...
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Phosphoproteomics of cellular mechanosensing reveals NFATC4 as a regulator of myofibroblast activitybioRxiv - Systems Biology 2023Quote: ... cleared by centrifugation and transferred to a clean 2 ml 96-well deep-well plate (DWP, Eppendorf, Hamburg, Germany). TiO2 beads (Titansphere® Phos-TiO Bulk 10 µm ...
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bioRxiv - Cell Biology 2023Quote: ... Oocytes were then microinjected with ~5 pl of mRNAs in M2 medium with 2.5 mM milrinone and 3mg/mL BSA at room temperature with a micromanipulator TransferMan NK 2 (Eppendorf) and picoinjector (Medical Systems Corp.) ...
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bioRxiv - Genomics 2022Quote: ... Supernatant was transferred using a wide-bore pipette to a 2 mL DNA low-bind tube (Eppendorf, Enfield CT), precipitated with 1 mL of 100% ethanol ...
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bioRxiv - Neuroscience 2023Quote: ... and tubes centrifuged at 4 °C for 2 min at 12000 x g in a refrigerated microfuge (Eppendorf 5415R). The upper phase was discarded ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was incubated at 25 °C for 2 h with intermittent mixing (Thermomixer comfort, Eppendorf, Wesseling-Berzdorf, Germany) to reduce viscosity ...
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bioRxiv - Biochemistry 2023Quote: ... Protein LoBind® microtubes (2 mL) and epT.I.P.S.® pipette tips (1000 µL) were purchased from Eppendorf (Hamburg, Germany).
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bioRxiv - Genomics 2024Quote: ... The resultant DNA was collected and stored in a 2 mL DNA LoBind® Tube (Eppendorf Cat. No. 022431048) at 4 °C until library preparation ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were detached by use of a cell scraper and transferred into suitable tubes (Eppendorf, Hamburg, Germany) containing 270 μL of MeOH (hypergrade for liquid chromatography ...
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bioRxiv - Biochemistry 2020Quote: ... cells were initially grown at 30°C to accumulate cell mass in an Innova R43 shaker (Eppendorf) at 250 rpm for 24-48 hours with the pH maintained at pH 5-6 ...
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bioRxiv - Cell Biology 2023Quote: ... Resuspended cells were then plated in 10 cm dishes and incubated inside a cell culture incubator (Eppendorf, Germany) at 37°C with 5% CO2 for ninety minutes ...
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bioRxiv - Molecular Biology 2023Quote: 10xGenomic single cell 3′ library was prepared as follows: single cells were sorted into 1.5 mL tubes (Eppendorf) and counted under the microscope ...
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bioRxiv - Cell Biology 2020Quote: ... the beads were separated from the protein extracts on a magnetic rack and transferred to a new 2 ml LoBind tube (Eppendorf). The beads were washed with 1 ml of each following solution ...
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bioRxiv - Cell Biology 2020Quote: ... The whole lane was excised from the gel and future cut into approximate 3 x 1 x 2 mm3 slices (L x W x H) and each slice were placed into a separate LoBind tubes (Eppendorf) for destaining ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was eluted with 2 x 100 μl of fresh elution buffer (100 mM dithiothreitol in RNase-free H20) directly into 2 ml lobind tubes (Eppendorf) containing 700μl Buffer RLT (RNeasy MinElute Cleanup Kit ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Soaked biofilms were gently removed from the agar surface using a sterile Drigalski spatula and a pipette tip and together with the unabsorbed stabilization mix transferred into a 2 mL tube (Eppendorf). An additional 1 mL of stabilization buffer was added to the tubes and the content was homogenized with a sterile pestle ...
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bioRxiv - Developmental Biology 2021Quote: ... the tissue samples were weighed (between 1.4 - 11.0 mg) and transferred into a 2 mL screw cap Eppendorf plastic tubes (Eppendorf, Stevenage, UK) along with a single 5 mm stainless steel ball bearing ...
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bioRxiv - Neuroscience 2022Quote: ... The homogenate was filtered through a 50 m.um filter (Sysmex; 04-004-2327) into a 2 mL microcentrifuge tube (Eppendorf; 022431048). An additional 0.5 mL of homogenization buffer was used to wash the Dounce homogenizer and filter ...
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bioRxiv - Biophysics 2020Quote: ... The needle was then glued to a 6×4×2 mm Neodyn magnet (QM-06×04×02-N, Magnets4you) and attached to a motorized micromanipulator (PatchMan, Eppendorf). The magnetic needle was lowered until it touched the bottom of a dummy sample dish and raised again so that it was placed above the bottom of the sample with the very tip in focus around 100 μm above the focus of the glass surface.
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bioRxiv - Molecular Biology 2019Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 2 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA.
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bioRxiv - Evolutionary Biology 2019Quote: ... pH 8.5 with 1 µg endoproteinase Lys-C (Wako) for 2 hours with shaking at 600 rpm at 37 °C in a thermomixer (Eppendorf). One µg of trypsin (Pierce ...
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bioRxiv - Microbiology 2021Quote: ... The surrounding SCFM was directly transferred to individual sterile 2 ml DNA lo-bind tubes from the 24-well plate (Eppendorf).
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bioRxiv - Biochemistry 2020Quote: ... Reactions were started by addition of 59.0 μM ubiquitin and 2 mM ATP then incubated at 37°C in a Mastercycler nexus (Eppendorf, Germany) thermocycler for 2 hrs unless otherwise stated ...
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bioRxiv - Biochemistry 2020Quote: ... we gently shook the flower and collected pollen into a weighed 2 mL microcentrifuge tube (Eppendorf®, Safe-Lock™). For species for which pollen was more difficult to harvest ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were kept on ice and centrifuged at 14 krpm for 2 min on a mini spin plus centrifuge (Eppendorf) before measurement ...
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bioRxiv - Neuroscience 2019Quote: ... supplemented with 3.5 µl 2-β mercaptoethanol was added directly to the beads and incubated with mixing on a ThermoMixer (Eppendorf) for 10 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μM purified TDP-43-MBP-His6 variants (WT, 5D, 12D, 12A) were set up in low binding tubes (Eppendorf) in 35 μl aggregation buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... pelleted by centrifugation (300 rcf, 3 min, RT in 50-mL conical tubes; then 21,000 rcf, 2 min, RT in Eppendorf tubes), and stored at -80 °C ...