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Citations for Eppendorf :
1551 - 1598 of 1598 citations for C Reactive Protein CRP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... as previously stated) they were transferred (three-quarters of a T25 flask/well) to non-adherent 24-well plates (Eppendorf, Hamburg, Germany, Cat #0030 722.019). Vero cells were plated in 24-well plates and then exposed to ZIKV at an MOI of 1 ...
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bioRxiv - Biophysics 2021Quote: ... Lipid extracts were resuspended in 60 μl 10mM ammonium acetate in methanol and diluted 1:4 in 96-well plates (Eppendorf twin.tec® 96; Sigma-Aldrich) prior to measurement of PC species ...
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bioRxiv - Biochemistry 2021Quote: ... After suspension it was subjected to centrifugation for 5 minutes at 5000 rpm in an Eppendorf 5804R centrifuge (Eppendorf, Hamburg, Germany) after which the supernatant was decanted into the waste ...
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bioRxiv - Bioengineering 2021Quote: ... CsupADH_17286 and HzeaADH7 were introduced into Agrobacterium tumefaciens GV3101 strain (MP90RK) by electroporation (1700 V mm-1, 5 ms, Eppendorf 2510). A viral silencing suppressor protein P19 was introduced into GV3101 strain as well in order to inhibit the host cells’ transgene silencing apparatus and extend transgene expression over a longer period of time with a higher degree of expression (Canto et al ...
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bioRxiv - Biochemistry 2022Quote: ... After 5 min incubation at room temperature (RT) the sample was centrifuged (8 min, RT, 180 x g, Eppendorf centrifuge 5810R) to separate proteoliposomes from the non-incorporated protein and detergent ...
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bioRxiv - Microbiology 2021Quote: ... overnight cultures in LB were spun down via centrifugation for 5 minutes at 4000 rpm at room temperature (Eppendorf 5810 R). The supernatant was filtered twice (Medical Millex-GS Filter ...
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bioRxiv - Neuroscience 2023Quote: ... Following that, the cells were washed thrice with 1mL Dulbecco’s DPBS and centrifugation (600 ⍰ g, 5 min, room temperature)(Eppendorf, cat # 5424R). Afterwards ...
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bioRxiv - Cancer Biology 2023Quote: ... transferred all the tissue fragments from the Petri dish into a clean 5 mL low-binding tube (Eppendorf, cat. no. 0030108.310), and rotated the tube at 20 rpm at room temperature for 15 min ...
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bioRxiv - Biochemistry 2020Quote: ... The protein solution was then kept on ice whilst the absorbance at 280nm was measured using a BioPhotometer® D30 (Eppendorf) spectrophotometer ...
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bioRxiv - Genomics 2022Quote: ... they froze and crushed a single, adult female spider with a pellet pestle (Fisher, #12-141-364) in a Protein LoBind tube (Eppendorf, #022431081) containing 200 uL of Buffer CT ...
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bioRxiv - Genomics 2022Quote: ... We flash froze adult spiders in liquid N2 and crushed them with a pellet pestle (Fisher, 12-141-364) in a Protein LoBind tube (Eppendorf, 022431081) containing 220 uL of Buffer ATL ...
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bioRxiv - Neuroscience 2021Quote: ... CSF samples were collected by pre-pulled glass capillary tube from the cisterna magna and then transferred to 0.5mL Protein LoBind Eppendorf tubes (Eppendorf Cat #022431064) for centrifugation at 12,700 rpm for 7 minutes at 4°C ...
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bioRxiv - Genomics 2021Quote: ... The pellet was resuspended in 400 μl 1XHB buffer and transferred to a 2 ml Eppendorf Protein Lo-Bind tube (Eppendorf, UK). 400 μl of 50% Iodixanol solution (Opti-Prep Density Gradient Medium ...
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bioRxiv - Genomics 2022Quote: ... the PCR products for apoLp and green fluorescent protein (GFP) were amplified from cDNA in a Mastercycler Nexus instrument (Eppendorf, Hamburg) with a 2-min initial denaturation at 94 °C followed by 30 cycles of 15 sec at 94 °C ...
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bioRxiv - Microbiology 2024Quote: ... The traps were washed three times with 150 µL S-Trap buffer and placed in clean 1.5 mL microcentrifuge tubes (Eppendorf Protein LoBind). Then 20 µL of 50 mM ABC containing 1 µg of trypsin (Promega Platinum Mass Spectrometry Grade ...
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bioRxiv - Bioengineering 2024Quote: ... BMP-2 solution was added to FN coated polymers and after 2 h incubation solution was aspirated and collected in Protein LoBind Tubes (Eppendorf™). Enzyme-linked immunosorbent assays (ELISA ...
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bioRxiv - Microbiology 2019Quote: ... four 50 mL aliquots from each trap were centrifuged at 3214 x g and 4°C for 30 minutes (Eppendorf 5810 R, S-4-104 rotor). The supernatant was decanted after centrifugation and cell pellets were stored at −80°C until ready for freeze-drying and lipid extraction ...
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bioRxiv - Bioengineering 2020Quote: ... nitric acid and incubating at 98°C for 2 h with shaking at 300 rpm in an Eppendorf® ThermoMixer® Comfort 5355 dry block shaker (Eppendorf UK Ltd, Stevenage, Herts, UK).
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bioRxiv - Microbiology 2021Quote: ... 1 μl of the lipid-containing lower organic phase was spotted on a MALDI target plate (Prespotted AnchorChip 96 Set for Proteomics II; Bruker Daltonics/Eppendorf; washed peptide-free with 2-propanol) pre-spotted with 1 µl of 9-aminoacridine (10 mg/ml dissolved in acetone:water 9:1 (v/v) ...
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bioRxiv - Developmental Biology 2021Quote: ... the heads were cleaned with alcohol and a 1μl single cell suspension (5 x 106 cells/ml) was manually injected directly into the left cerebellar hemisphere using a glass microcapillary (Eppendorf, 5195 000.079) controlled with an Eppendorf CellTram Vario manual microinjector ...
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bioRxiv - Microbiology 2020Quote: ... The overnight culture was diluted in Mueller Hinton Broth (MHB) based on the OD600 to obtain 5 × 105 CFU/mL using a BioPhotometer D30 (Eppendorf, Hauppauge, NY). An OD600 of 1 was assumed to be equal to 8 × 108 CFU/mL.
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bioRxiv - Evolutionary Biology 2022Quote: ... We separated the tissue from the buffer and reproductive fluid by centrifuging at 4ºC for 5 min at 3500 rpm (Eppendorf Centrifuge 5702RH, USA), pipetted the supernatant from each tract region into microplate wells (CellStar 96 Well Cell Culture Plate ...
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bioRxiv - Microbiology 2020Quote: ... we centrifuged a 0.5-mL sample of culture in the exponential phase at 7000 rpm for 5 min in a Benchtop centrifuge (5424 Eppendorf, Hamburg, Germany). We washed the pelleted biomass 1 – 2 times and resuspended it with 50 µL 1x PBS from which we fixed 2 µL on solidified agarose (VWR ...
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bioRxiv - Cancer Biology 2023Quote: For automated dual luciferase reporter assay 2-2.5 x 104 HEK 293 T cells were seeded per 96-well by the liquid handling system epMotion 5075 (Eppendorf, Hamburg, Germany). HEK 293 T cells were transfected with 50 ng/well reporter vector with or without 3′UTR and 200 ng/well pSG5 empty vector or pSG5-miR-142-5p expression plasmid ...
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bioRxiv - Genomics 2023Quote: ... The bone pieces in 5 ml tube of DNA Lobind were treated pre-digestion for 15 minutes at 900 rpm in a Thermomixer (Eppendorf, Hamburg, Germany) with the lysis buffer (2 ml ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were diluted to 2 x 107 cells per mL and 50 µL of each sample was added to 5 mL tubes (Eppendorf, Hamburg, DE), to constitute 1 million cells per flow sample.
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bioRxiv - Genomics 2020Quote: ... 3X in Ribohyb buffer and resuspended in a final volume of 10 ul Ribohyb buffer in a non-stick tube (Eppendorf Protein LoBind). The 10 µl hybridization reactions were added to 10 µl of resuspended beads for a binding reaction volume of 20 µl ...
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bioRxiv - Biochemistry 2021Quote: ... all samples were adjusted to the same volume and concentration (ca. 1 mg/mL) and transferred to protein low-bind microcentrifuge tubes (Eppendorf, Hamburg, Germany). To precipitate the proteins ...
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bioRxiv - Neuroscience 2020Quote: Aggregate enrichment was performed by centrifugation of a total of 150 μg protein at 20’800 g in a tabletop centrifuge (Eppendorf 5417r, Hamburg, Germany) for 1 hour at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... The protein samples of N-NTD and N-NTD-SR at 20 µM were prepared in low-binding microtubes (Eppendorf® LoBind) containing 20 mM Tris-HCl buffer (pH 7.5 ...
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bioRxiv - Systems Biology 2022Quote: ... each SCN was cut into 3 pieces with a scalpel on a glass slide and transferred to a protein LoBind tube (Eppendorf, Hamburg, Germany) prefilled with 250 µL lysis buffer (100 mM Tris-HCl ...
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bioRxiv - Genomics 2023Quote: ... approximately 1.0-2.0 x 105 GFP+ neurons per pup were collected by FACS into a 1.5 mL Protein LoBind Tube (Eppendorf, cat. no. #Z666505).
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bioRxiv - Biochemistry 2023Quote: ... Samples were thawed and Urea was added to a final concentration of 8 M urea and the protein lysate was transferred to 2mL snap-cap centrifuge tubes (Eppendorf, Hamburg, Germany) with 0.1 mm zirconia beads and bead beat in a Bullet Blender (Next Advance ...
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bioRxiv - Biochemistry 2019Quote: Vero cells were harvested by trypsinization when it reached 80-90% confluence and centrifuged at 1000 × g for 5 mins (Eppendorf Centrifuge 5804R, Germany). The clean Vero cell pellet was resuspended thoroughly with growth medium (GM ...
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bioRxiv - Microbiology 2020Quote: ... 20 mL of the enrichment was transferred to 5 L NMS medium in a 6-L fermenter controlled with a BioFlo® 120 Bioprocess Control Station (Eppendorf, Hamburg, Germany). Gas stream carrying a relatively low CH4 concentration (0.5% v/v in air ...
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bioRxiv - Microbiology 2024Quote: ... and 40 mL of the supernatant was added as the inoculum to 5 L medium prepared in a BioFlo® 120 bioreactor (Eppendorf, Hamburg, Germany). Air containing 0.5% or 10% (v/v ...
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bioRxiv - Systems Biology 2024Quote: ... placed in a 2mL Protein LoBind® Eppendorf tube and centrifuged at 1000g for 2 minutes at RT (Microcentrifuge 5415, Eppendorf, Hamburg, Germany).
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bioRxiv - Biochemistry 2021Quote: ... The molecular weight cut-off columns were rinsed by addition of equal volumes of Milli-Q water and the proteins were dried with a speed-vac (Eppendorf, Vacufuge Concentrator, Stevenage, UK).
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bioRxiv - Biochemistry 2022Quote: ... the supernatant was removed and cells were resuspended in cold PBS at 20 million cells/mL and then distributed into 1.5 mL microcentrifuge tubes (Eppendorf Protein LoBind tubes (Z666505-100EA) at 1 mL each ...
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bioRxiv - Biochemistry 2023Quote: ... Polypropylene standard safe-lock microcentrifuge vials and corresponding low protein binding vials of 1.5 mL size from three manufacturers were used: Manufacturer A (Eppendorf Safe-Lock Tubes no. 0030120086 and Protein LoBind® Tubes no. 0030108116, Eppendorf SE, Hamburg, Germany), Manufacturer B (Pierce™ Microcentrifuge Tubes no ...
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bioRxiv - Molecular Biology 2021Quote: ... Flowrate was adjusted at 2 ml/min to collect peptides in 1 ml fractions in low-protein binding Eppendorf tubes (Eppendorf LoBind tubes, cat. no. EPPE0030108.116). The bound complexes were separated from β2 microglobulin and heavy chain using increasing concentration of buffer B ...
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bioRxiv - Microbiology 2021Quote: ... following the standard protocol in this kit and liquid handling in Eppendorf epMotion 5075 (Eppendorf North America). The extracted RNA was transcribed into cDNA according to a standard protocol using iScript™ cDNA Synthesis Kit (BIO-RAD ...
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bioRxiv - Neuroscience 2021Quote: ... the circular DNA fragment containing the shotV104 breakpoint region was amplified using a High Fidelity PCR Kit (Eppendorf and Roche). PCR products were gel-extracted ...
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bioRxiv - Genomics 2020Quote: ... samples were processed according to the Qiagen PowerMag Microbiome DNA/RNA Isolation Kit protocols on an epMotion 5073 liquid handling robot (Eppendorf). Extracted DNA was quantified using the QIAxpert A260 dsDNA system (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... The reagents were mixed as per the QuikChange II XL Site-Directed Mutagenesis Kit instructions and loaded into a Mastercycler pro S (Eppendorf) with the settings recommended by the manual ...
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bioRxiv - Microbiology 2024Quote: Sample were retrotranscribed in cDNA using 1 µL of RNA with 1 µL of Reverse Transcription Master Mix and 3 µL of RNase-free ultrapure water provided with the kit (Standard Biotools, USA) using a thermal cycler (Eppendorf, Germany) with the following cycles ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... GoScript Reverse Transcriptase) provided by the kit was added to start the reverse transcription reaction in the thermocycler (Eppendorf Flexide Mastercycler Nexus) for 5 min at 25°C ...
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bioRxiv - Molecular Biology 2023Quote: ... we favoured magnetic beads to perform DNA extraction and lysates were processed for DNA extraction using the Macherey-Nagel™ NucleoMag® Tissue kit on an epMotion® 5075vt (Eppendorf, Hamburg – Germany). Volumes on the first binding step were adjusted to the starting volume of lysis buffer accordingly ...