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Citations for Eppendorf :
1301 - 1350 of 1356 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Each sample was processed on an independent Chromium Single Cell A Chip (10X Genomics) and run on a thermocycler (Eppendorf). 5’ gene expression libraries were sequenced using NextSeq 500 150-cycle high-output flow cells or NovaSeq 6000 200 cycle S4 flow cells ...
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bioRxiv - Microbiology 2024Quote: ... cells were transfected with both TAP amplifications in a final volume of 1 ml into 96 deep well plate (Eppendorf), and after 7 days of expression mAb-containing supernatants were harvested by centrifugation.
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bioRxiv - Cell Biology 2023Quote: ... guided through a microinjection needle holder was placed just above the cells of interest by means of a microinjection micromanipulator InjectMan NI2 (Eppendorf). LED590 (ThorLabs M590F2 ...
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bioRxiv - Microbiology 2024Quote: ... The crude cell lysate was refined by centrifuging at 18514 g for 40 min (Fixed angle rotor, 5810-R Centrifuge, Eppendorf). The clarified lysate was applied to a 5 mL HisTrap™TALON™ crude cobalt column (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... A volume of 1 nL containing 500 ng/μL mRNA coding for each of these proteins was injected into one-cell stage embryos using the FemtoJet 4i microinjector (Eppendorf). H2O was used as reference control.
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bioRxiv - Biochemistry 2024Quote: ... Cells were then pelleted by centrifugation at 3900 × g for 20 min (5810R centrifuge with S-4-104 rotor, Eppendorf) and stored at −20 °C.
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bioRxiv - Molecular Biology 2024Quote: ... was injected into the cytoplasm of 1-cell embryo in a droplet of M2 medium using a FemtoJet microinjector (Eppendorf) with constant flow settings ...
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bioRxiv - Cell Biology 2024Quote: ... The culture was maintained in a humidified atmosphere containing 5% CO2 at 37 L in a cell incubator (Eppendorf, Germany). The medium was changed daily ...
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bioRxiv - Developmental Biology 2024Quote: ... One nL morpholino (dissolved in 0.2 M KCl with 0.05% phenol red at 3 mg/mL) was microinjected into haploid embryos at the 1 or 2-cell stage using FemtoJet and InjectMan NI2 (Eppendorf).
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bioRxiv - Genomics 2024Quote: ... and washed in nuclease-free H2O before proceeding to MNase digestion with 1-2U of MNase for 3×106 cells that went on for 1-hour at 37C while shaking in a thermomixer (Eppendorf) at 550rcf ...
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bioRxiv - Microbiology 2023Quote: ... 2ml of cells were collected at different time points and was centrifuged at 2,348g (FA-45-24-11, Eppendorf rotor) for 5 minutes at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the growth medium was aspirated and Cultrex droplets were gently dislodged into the cell recovery solution (Cat. No. 354270, Corning and transferred to Eppendorf tubes using wide-bore 1 ml pipette tips ...
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bioRxiv - Genomics 2023Quote: ... gating was established and single cells were sorted (130 micron nozzle assembly) into low-bind 96 well PCR plates (Eppendorf twin.tec LoBind ...
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bioRxiv - Neuroscience 2023Quote: ... The 1 ml of PBS with the resuspended cells was transferred to a 1·5 ml Protein LoBind tube (Eppendorf), centrifuged at 3000 x g for 6 min at 4 °C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We collected these bacteria into KK2 with a sterile loop and diluted them to 1.5 OD600 in KK2 (∼5 × 108 cells, measured with an Eppendorf BioPhotometer). We used these bacteria as food for amoebas in our experiment and repeated this process anew for each of the three replicate experiments.
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bioRxiv - Genetics 2023Quote: Zebrafish embryos were collected and injected as previously described (Rosen et al., 2009) at one-cell stage using a FemtoJet Injector (Eppendorf) or PV820 injector (WPI ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We collected these bacteria into KK2 with a sterile loop and diluted them to 1.5 OD600 in KK2 (∼5 x 108 cells, measured with an Eppendorf BioPhotometer). We used these bacteria as food for amoebas in our experiment and repeated this process anew for each of the three replicate experiments.
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bioRxiv - Cancer Biology 2024Quote: ... 5 nl of the cell solution was injected into the perivitelline space (PVS) of each embryo utilizing an Eppendorf microinjector (Femto-Jet 5247, Eppendorf) and a Manipulator MM33-Right (Märzhäuser Wetziar) ...
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bioRxiv - Cell Biology 2024Quote: ... Huh7 cells were grown in 24-well glass bottom plates (170 μm coverglass bottom; Eppendorf, 0030741021; Cellvis, P24-1.5H-N). Cells were either untreated or incubated in 100 μM oleate-BSA complex for 24 hr ...
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bioRxiv - Biophysics 2021Quote: ... The monolayer was formed by seeding the suspended cells in the centre of a 35 mm diameter glass-bottom Petri dish (Eppendorf®). After the formation of the confluent monolayer ...
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bioRxiv - Cell Biology 2020Quote: ... The micromanipulator was installed on the microscope allowing for immediate cell microscopy after the injection and was connected to the microinjector FemtoJet (Eppendorf, Germany). The microinjection was performed at 20-30 hPa injection pressure ...
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bioRxiv - Zoology 2019Quote: ... Testicular and ovarian cell suspension was loaded into the glass capillary attached to MN-153 micromanipulator (Narishige) and FemtoJet® 4x injector (Eppendorf). Triploid recipients were injected by approximately 3000-5000 of testicular cells (TC group ...
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A sound strategy for homology modeling-based affinity maturation of a HIF-1α single-domain intrabodybioRxiv - Biochemistry 2020Quote: ... Escherichia coli TG1 cells were electroporated (2.5 kV, 25 μF, 200 Ω) with the linked products using an Eppendorf Eporator® instrument (Eppendorf). Colony PCR and sequence analysis (GENEWIZ ...
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bioRxiv - Cancer Biology 2020Quote: ... Single LRCs and nLRCs were isolated as single PKH-positive/DAPI-negative and PKH-negative/DAPI-negative cells using a micromanipulator (Eppendorf PatchMan NP2, Eppendorf, Germany) or flow cytometric activated cell sorting ...
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bioRxiv - Plant Biology 2020Quote: ... Bacterial pellets obtained from 1 mL of cell culture centrifuged at 13,000 rpm (Eppendorf 5424 Centrifuge with Fa-45-11 Rotor) were thoroughly washed three times with 1 mL fresh LB medium to remove residual antibiotics and were re-suspended in 100 µL liquid LB medium ...
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bioRxiv - Genetics 2022Quote: ... solution containing capped mRNAs (green fluorescence protein [GFP]mRNA or mutant pls1 mRNA or wildtype pls1 mRNA) was injected into the zebrafish embryos (one-cell stage) using FemtoJet 4i (Eppendorf, Germany). Embryos at different developmental stages were preserved and collected for subsequent experiments
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bioRxiv - Microbiology 2022Quote: ... Targeted cells were collected using capillary-based micro-manipulation with glass capillaries containing an inner diameter of 8µm (Eppendorf, Hamburg, Germany). Capillaries were pre-coated with a sterile 2% BSA solution to prevent the gametes from sticking to the glass capillary surface ...
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bioRxiv - Genomics 2024Quote: ... The grown bacterial cells were subsequently centrifuged at 5000 G for 10 min using a 5920 R centrifuge (Eppendorf, Hamburg, Germany) washed in 1 mL 0.9% NaCl and subsequently centrifuged again at 12000 rpm for 3 min ...
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bioRxiv - Bioengineering 2024Quote: ... the EV-conditioned medium was collected in polypropylene tubes and centrifuged at 300 ×g for 11 min followed by 2000 ×g for 22 min to pellet down apoptotic bodies and cell debris using an Eppendorf 5810 R 15 amp centrifuge (Eppendorf, Germany). The supernatant was transferred to polycarbonate tubes and centrifuged at 20,000 ×g for 45 min at 4°C to pellet MVs using a Sorvall MX 120+ micro-ultracentrifuge (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... then 1 mL aliquots were placed in triplicate in plastic-bottomed black 24-well plates (Eppendorf Cell Imaging Plates ref. 0030741005), and the plates were placed onto a custom made light plate apparatus (LPA)27 to control blue light illumination in each well (λ = 460 nm ...
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bioRxiv - Cell Biology 2023Quote: ... Cell injection was performed by using a micromanipulator (Inject Man) and an oil-driven microinjector (CellTram vario) (both Eppendorf, Wesseling, Germany).
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bioRxiv - Neuroscience 2023Quote: ... Following that, the cells were washed thrice with 1mL Dulbecco’s DPBS and centrifugation (600 ⍰ g, 5 min, room temperature)(Eppendorf, cat # 5424R). Afterwards ...
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bioRxiv - Immunology 2021Quote: ... cell populations were distinguished by WGA and DRAQ5 signals and with individual cells sorted into each well of a 384-well plate (twintec™ PCR plates, Eppendorf, Germany) containing 2.3 µl lysis buffer (Picelli et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... the heads were cleaned with alcohol and a 1μl single cell suspension (5 x 106 cells/ml) was manually injected directly into the left cerebellar hemisphere using a glass microcapillary (Eppendorf, 5195 000.079) controlled with an Eppendorf CellTram Vario manual microinjector ...
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bioRxiv - Neuroscience 2021Quote: ... The cells stably expressing H-Luc-NLS were plated on a black flat glass bottom 96-well plates (Eppendorf; tissue culture treated). They were incubated with 1 μM MaPCahigh AM dyes and 0.04% Pluronic-F127 for at least 2 h in 100 μL at 37°C ...
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bioRxiv - Biophysics 2020Quote: ... 200 ml of M9 minimal media were added to 4 mL of pre-inoculum and the cell growth was monitored using Biophotometer D30 (Eppendorf, Hamburg, Germany) until the growth rate was 0.8 (OD at 600 nm) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were recovered from each well by centrifugation at 6000 g for 15 minutes using an Eppendorf 5424 Microcentrifuge (Eppendorf, Hamburg, Germany). The cell pellet was dissolved in 250 µL of DMSO ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... containing 100 ng/μL of Cas9 mRNA and 25 ng/μL of sgRNA was injected into Japanese medaka 39 embryos at the one-cell stage using Femto Jet (Eppendorf, Hamburg, Germany) and GD-1 needles (Narishige ...
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bioRxiv - Genetics 2020Quote: ... Approximately 3 nl of RNP complex were injected into the animal pole of one-cell stage embryos (50-250 embryos/experiment) using a Femtojet 5247 microinjector (Eppendorf, Hamburg, Germany) under a Nikon DS-Ri2 stereomicroscope ...
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bioRxiv - Cell Biology 2021Quote: Live-cell microinjection experiments were performed using a FemtoJet® 4i microinjector in conjunction with an InjectMan® 4 micromanipulation device (Eppendorf). All microinjections were performed using pre-pulled Femtotips® injection capillaries (Eppendorf) ...
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bioRxiv - Neuroscience 2023Quote: ... The plasmid injection solution was injected into Hydra vulgaris AEP 1-cell stage embryos using an Eppendorf FemtoJet 4x and Eppendorf InjectMan NI 2 microinjector (Eppendorf; Hamburg, Germany) under a Leica M165 C stereo microscope (Leica Microscopes ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were diluted to 2 x 107 cells per mL and 50 µL of each sample was added to 5 mL tubes (Eppendorf, Hamburg, DE), to constitute 1 million cells per flow sample.
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bioRxiv - Microbiology 2023Quote: ... Pre-weighted metal-free 15 mL propylene tubes were used to pellet the cells using a prechilled tabletop centrifuge (Eppendorf, Hauppauge, NY). Pellets were washed three times with 10 mL of ice-cold PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... 100 ng corresponding pEcgRNA-N20 and 400 ng donor DNA were co-electrotransformed into 100 uL competent cells in 1 mm gap electroporation cuvette by applying 2.5 kV pulse in electroporator (Eppendorf™ Eporator™). After adding 900 uL of cold SOC medium ...
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bioRxiv - Cell Biology 2024Quote: ... The donor cell suspension was loaded into the injection needle through mouth pipetting and subsequently affixed to a microinjector (FemtoJet; Eppendorf, Hamburg, Germany). Each placenta was punctured by an injection needle to a depth of 2 mm ...
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bioRxiv - Biochemistry 2019Quote: Vero cells were harvested by trypsinization when it reached 80-90% confluence and centrifuged at 1000 × g for 5 mins (Eppendorf Centrifuge 5804R, Germany). The clean Vero cell pellet was resuspended thoroughly with growth medium (GM ...
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bioRxiv - Biochemistry 2021Quote: ... Production of CjLPMO10AFL was achieved by fed-batch fermentation of the expression strain in a 1-liter fermenter (DASGIP® benchtop bioreactors for cell culture; Eppendorf, Hamburg, Germany), essentially as described previously (44) ...
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bioRxiv - Microbiology 2021Quote: The rest of the supernatant cell suspension (~19 ml) was centrifuged in a swing-out rotor (Eppendorf A-4-62 Swing Bucket Rotor) at 3200 × g for 10 min to pellet cells ...
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bioRxiv - Developmental Biology 2024Quote: ... One- or 2-cell stage embryos were injected with approximately 20 nl of the injection mixture (manually or using an Eppendorf FemtoJet 4x microinjector) and maintained at 20°C until the 64-cell stage ...
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bioRxiv - Developmental Biology 2021Quote: ... or double (for qRT-PCR) blastomere microinjections of 2-cell (E1.5) stage embryos were performed using the FemtoJet 4i (Eppendorf, Hamburg, Germany; Cat. No. 5252000013) micro-injector ...