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Citations for Eppendorf :
851 - 900 of 1356 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... and extension at 72°C for 1 min followed by final extension was conducted at 72°C for 5 min using a PCR minicycler (Eppendorf Ltd., Germany). After amplification ...
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bioRxiv - Molecular Biology 2019Quote: ... The resulting precipitates were centrifuged for 15 minutes at 25000 relative centrifugal force (rcf) in a 5417R Eppendorf centrifuge at 4°C (Eppendorf, Hamburg, Germany). The resultant supernatants were equilibrated to room temperature and then carefully drawn and added into fresh 1.5ml tubes containing 400ml of isopropanol ...
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bioRxiv - Genomics 2020Quote: ... We included an overnight digestion step in which tissues were lysed in a 96-well PCR plate at a constant temperature of 55° C on an Eppendorf Mastercycler thermal cycler (Eppendorf, Hamburg, Germany), and we included 10 uL Proteinase K solution ...
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bioRxiv - Immunology 2021Quote: ... The 80 µL aliquot was lyophilised for 4 h at 30 °C with the aid of a vacuum concentrator (Eppendorf Concentrator 5301) attached to a refrigerated condensation trap (Savant) ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were maintained in a humidified atmosphere of 5% CO2 and 37 °C and were passaged every 2-3 days into 10 cm polystyrene coated plates (Eppendorf; EP0030700112-300EA) upon reaching high density ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were eluted with 60 μl of 80% acetonitrile/1% ammonia and dried at 60°C using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Dried peptides were dissolved and sonicated in 5% acetonitrile/0.1% TFA ...
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bioRxiv - Systems Biology 2021Quote: ... Total volume of 20 mL from each culture was transferred to a 50 mL Falcon® filled with ice and was immediately centrifuged at 3000 rpm for 3 min at 4 °C (Eppendorf centrifuge). Next the supernatant was discarded and the cell pellet was snap frozen into liquid nitrogen and stored at -80 °C ...
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bioRxiv - Systems Biology 2021Quote: For the extraction of total proteome 10 mL of each culture were transferred into ice-cold 15 mL Falcon® tubes which were centrifuged immediately at 3000 rpm for 3 min at 4 °C (Eppendorf centrifuge). The supernatant from the centrifugation was discarded and the cell pellets were washed once with 1 mL of cold PBS buffer ...
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A Polybasic Domain in aPKC Mediates Par6-Dependent Control of Membrane Targeting and Kinase ActivitybioRxiv - Cell Biology 2020Quote: ... Approximately 22.5×107 suspension-adapted Expi293F cells were diluted in Expi293 Expression Medium to the final volume of 76.5 ml and cultured in a 37°C shaker (Eppendorf New Brunswick S41i). 90µg of plasmid DNA was mixed with 240µl Expifectamine reagent into a total final volume of 9ml OPTI-MEM medium and was incubated for 25 min ...
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bioRxiv - Genetics 2022Quote: ... we inoculated yeast strains into 400 µL of liquid SC -lys medium with G418 for overnight growth in 2 mL 96 well plates at 30 °C with 1000 rpm mixing on a MixMate (Eppendorf, Hamburg, Germany). The next day ...
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bioRxiv - Synthetic Biology 2022Quote: ... The fluorocarbon/ethanol mix was shaken at room temperature for 16 h with 1000 rpm using an Eppendorf ThermoMixer C (Eppendorf AG, Germany) to perform the liquid-liquid extraction ...
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bioRxiv - Systems Biology 2022Quote: ... Peptides were eluted with 60 μl of elution buffer (80% acetonitrile/1% ammonia) and dried at 60°C using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Dried peptides were redissolved and sonicated in 5% acetonitrile/0.1% TFA ...
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bioRxiv - Biophysics 2022Quote: ... pH 7.4) with and without 1,4 dithiothreitol (DTT) (10 mM) and agitated at 400 oscillations per minute (Eppendorf Thermomixer C, Eppendorf, Germany) for 48 h.
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bioRxiv - Plant Biology 2021Quote: ... 0.1 M NaHCO3) by incubating at 65°C for 15 min at 1200 rpm in a ThermoMixer Comfort (Eppendorf AG, Hamburg, Germany). NaCl was added to the eluates and the input DNA aliquots to a final concentration of 0.2 M ...
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bioRxiv - Cancer Biology 2020Quote: ... sonicated for 15 min and vigorously mixed at 5°C for 30 min with 2000 rpm on a ThermoMixer (Eppendorf, Hamburg, Germany). Samples were centrifuged for 5 min at 18000 rpm ...
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bioRxiv - Microbiology 2022Quote: ... 4.6×106 LMBs or NMBs were added and samples incubated at 37 °C for 1 hour on a thermomixer (60 rpm; Eppendorf, Hamburg, Germany). After this step ...
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bioRxiv - Developmental Biology 2022Quote: ... containing 50 pmols of each primer set (up to two channels per experiment) and 10% formamide] 16-48 hrs at 37°C mixing at 400 rpm in a thermomixer (Eppendorf ThermoMixer F1.5). Embryos were washed for 30 min in Stellaris Wash Buffer A ...
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bioRxiv - Genomics 2022Quote: ... The remaining PIP emulsion was subjected to enzymatic lysis at 65°C for 35 min on a PCR thermocycler (Eppendorf Mastercycler Pro) with lid temperature set to 105°C ...
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bioRxiv - Synthetic Biology 2019Quote: ... was added to each library and incubated for two hours at room temperature while shaking using a Thermomixer C (Eppendorf, Hamburg, Germany). M2B2 beads (US Biological ...
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bioRxiv - Cell Biology 2021Quote: ... in 25 mM AB) containing 50 µg of lysate protein and shaken overnight in a thermomixer (Eppendorf; 1400 rpm at 37 °C).
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bioRxiv - Microbiology 2020Quote: ... Liquid cultures were grown at 29°C and shaken at 200 and 180 rpm in New Brunswick Innova 2300 and Excella E25 shaking incubators (Eppendorf, Hauppauge, NY), respectively ...
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bioRxiv - Bioengineering 2021Quote: ... of stock cells were grown overnight in 5 mL of LB at 37°C and 200 rpm on a 75° angled platform in a New Brunswick Excella E25 (Eppendorf, CT, USA). Alternatively ...
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bioRxiv - Immunology 2021Quote: ... Lysates (5 μg) were incubated with diverse ZnCl2 or PP2A inhibitors for 30 min at 30 °C using a Thermo-mixer (Eppendorf, Hamburg, Germany). The protein samples were incubated for 30 min at 33 °C with a chemically synthesized phosphopeptide [RRA(pT)VA] ...
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bioRxiv - Microbiology 2022Quote: ... 20 µl supernatant was mixed with 180 µl substrate solution and incubated for 10 min at 37°C and 1000 rpm in a thermomixer (Eppendorf Thermomixer comfort). The reaction was stopped by addition of 60 µl 1 M sodium hydroxide ...
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bioRxiv - Immunology 2022Quote: ... Purified IgG was digested into F(ab’)2 with 200 μg of IdeS per 10 mg of IgG for 6 hr on a thermal mixer (Eppendorf ThermoMixer C) at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... The resin was pelleted down by centrifugation at 4°C (1258 g for 5 min, A-4-81 rotor, Eppendorf, Enfield, CT) and washed with ice-cold 50 mL Binding buffer composed of 10mM imidazole (pH 7.4) ...
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bioRxiv - Biophysics 2022Quote: ... and 6.25 A260 rRNA (23S rRNA + 5S rRNA) were mixed on ice and incubated at 44 °C using a thermo-block (Eppendorf, Thermomixer comfort) in Rec4 buffer (20 mM HEPES/KOH pH7.6 ...
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bioRxiv - Cell Biology 2022Quote: ... The crude extract was centrifuged for 15 min at 13,000 rpm at 4 °C to remove any tissue debris and the clear supernatant was transferred to a fresh Protein Low-Bind tube (Eppendorf, Hamburg, Germany). The total protein content of the samples was estimated using Pierce BCA protein assay kit from Thermo Scientific (Rockford ...
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bioRxiv - Cell Biology 2022Quote: The beads were resuspended with 40 µL extension mix and incubated at 37°C on a PCR cycler (Eppendorf, UX-93944-39) for 10 minutes ...
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bioRxiv - Synthetic Biology 2022Quote: ... flasks were incubated at 37°C and 200 rpm in a New Brunswick I24 Benchtop Incubator Shaker (Eppendorf North America, Enfield, CT) until an optical density of ~0.3-0.5 was reached ...
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bioRxiv - Physiology 2024Quote: ... and added with 150 μl of collagenase solution to each tube followed by incubation at 37°C with shaking for 10 min (750 rpm, Eppendorf ThermoMixer F1.5). The samples were then washed 2 times with 1X PBS-Tween (pH 7.2 ...
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bioRxiv - Microbiology 2024Quote: ... The culture supernatant was collected by centrifugation (6,000 rpm, 10 min, 4°C; R10A2 rotor, Himac CR21F; Eppendorf Himac Technologies, Hitachinaka, Japan). For cultures grown under photoautotrophic conditions ...
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bioRxiv - Microbiology 2024Quote: ... and incubated for 10 min on ice before being heated for 15 min at 95 ºC in a ThermoMixer C (Eppendorf, Mississauga, ON) at 350 RPM ...
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bioRxiv - Bioengineering 2023Quote: ... The recovered spores were washed by repeating the following steps for three times: (i) centrifugation at 4000 rpm at 25 °C for 10 min (5810 R, Eppendorf, Hamburg, Germany), (ii ...
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bioRxiv - Microbiology 2024Quote: ... one with the blood mixed to RPMI and the second with the gametocyte suspension were spun down at 2000rpm for 5 minutes in a temperature-controlled centrifuge at 37°C (Eppendorf 5702 R). RPMI supernatant was removed from both tubes with 3ml disposable Pasteur pipettes ...
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bioRxiv - Plant Biology 2024Quote: ... SDS at a 1:3 (w/v) ratio and extracted by shaking 1000 RPM 95 °C for using a tabletop shaker (Eppendorf ThermoMixer F2.0). Samples were then centrifuged at 20,000 xg for 10 min at room temperature and the supernatant retained in new tubes ...
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bioRxiv - Genetics 2024Quote: ... The samples were sonicated on ice for 12 to 15 minutes and then centrifuged for 10 minutes at 13200 rpm and 4 °C (Eppendorf centrifuge 5415R) to remove the cell debris ...
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bioRxiv - Biochemistry 2024Quote: ... Constructs were expressed in 1 L LB/kanamycin medium and grown at 37 °C while shaking at 180 rpm (Eppendorf Innova S44i) until an OD600 of 0.6 was reached ...
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bioRxiv - Bioengineering 2024Quote: ... 500 µl of the bacterial samples were then kept at 98°C for 15 min to undergo lysis in a static thermomixer (Eppendorf GmbH, Germany) according to the reported protocol (Škulj et al. ...
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... the samples were centrifuged at 13 000 rpm for 10 min at 10°C with Centrifuge 5424 R (Eppendorf AG, Hamburg, Germany). The clear supernatant was transferred to a 1.5 mL glass vial with insert ...
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bioRxiv - Biochemistry 2024Quote: ... The microtiter plates were closed with a transparent sealing tape to prevent evaporation of buffer solutions and incubated at 25 °C for 70 hours using a thermomixer (Eppendorf, Hamburg, Germany). After the incubation ...
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bioRxiv - Biochemistry 2024Quote: ... by adding 1000 µL Milli-Q water and incubating for 30 min at 30 °C and 1000 rpm in a ThermoMixer (Eppendorf, Hamburg, Germany). Then ...
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bioRxiv - Microbiology 2023Quote: ... The inoculated TSB was incubated at 37 °C for overnight and washed on the next day by centrifugation (Eppendorf, Hauppauge, NY, USA) at 3,000 × g for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... and the upper phase (water-soluble metabolites) was evaporated for 30 minutes at 35°C under nitrogen and dried in SpeedVac (Eppendorf, Hamburg, Germany) at 15 °C overnight ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial mixtures were diluted 1:1000 into LB with or without 1% DMSO (v/v) and incubated standing at 37°C in 5% CO2 at atmospheric oxygen (normoxic; Eppendorf CellXpert incubator), 1% oxygen (hypoxic ...
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bioRxiv - Microbiology 2023Quote: Liquid samples in 1 ml were collected and centrifuged for 20 min at 21 130 × g at 4°C (Centrifuge 5424 R; Eppendorf, Hamburg, Germany). The supernatants were used for chemical analyses by applying external standards for calibration and quality control ...
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bioRxiv - Zoology 2023Quote: ... The homogenates were centrifuged for 10 minutes (860 g at 4 °C) to remove the solid particulates (5810R, Eppendorf centrifuge, Hamburg, Germany) and the supernatant was removed and stored at -80 °C for later analysis [19] ...
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bioRxiv - Physiology 2023Quote: ... Microtubes were briefly vortexed and spun to collect volume at bottom of tube prior to incubation for 60 minutes at noted temperatures in a thermomixer (Eppendorf ThermoMixer C). Microtubes were immediately transferred and centrifuged at 20,000xg for 60 minutes at +25°C ...
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bioRxiv - Biophysics 2023Quote: ... This pre-gel solution was kept at 4 °C inside an amber UV-protected 1.5 mL microcentrifuge tube (Eppendorf®, Hauppauge, NY), and degassed for 1 h inside a vacuum chamber to reduce oxygen concentration and prevent early gelation inside the driving syringe while running the experiment ...
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bioRxiv - Developmental Biology 2023Quote: ... the gRNA:Cas9 ribonucleoprotein complex solution was incubated at 37°C for 5 min and then backfilled into 3 microinjection needles using an Eppendorf GELoader tip (Eppendorf, Cat# 022351656). After loading embryos into the embryo holder which covered with 12.5 ppt of salinity water with 0.0001% of methylene blue ...