Labshake search
Citations for Eppendorf :
951 - 1000 of 1278 citations for 6 propan 2 yl 1 3 5 triazine 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... coli S17-1 that contained the shuttle- or suicide-vector construct at 3700 rpm for 10 min at room temperature (Centrifuge 5920 R, rotor S-4×1000, Eppendorf, Hamburg, Germany). We mixed the E ...
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bioRxiv - Cell Biology 2021Quote: Live-cell microinjection experiments were performed using a FemtoJet® 4i microinjector in conjunction with an InjectMan® 4 micromanipulation device (Eppendorf). All microinjections were performed using pre-pulled Femtotips® injection capillaries (Eppendorf) ...
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bioRxiv - Microbiology 2024Quote: ... The culture supernatant was collected by centrifugation (6,000 rpm, 10 min, 4°C; R10A2 rotor, Himac CR21F; Eppendorf Himac Technologies, Hitachinaka, Japan). For cultures grown under photoautotrophic conditions ...
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bioRxiv - Genetics 2024Quote: ... The samples were sonicated on ice for 12 to 15 minutes and then centrifuged for 10 minutes at 13200 rpm and 4 °C (Eppendorf centrifuge 5415R) to remove the cell debris ...
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bioRxiv - Zoology 2023Quote: ... The homogenates were centrifuged for 10 minutes (860 g at 4 °C) to remove the solid particulates (5810R, Eppendorf centrifuge, Hamburg, Germany) and the supernatant was removed and stored at -80 °C for later analysis [19] ...
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bioRxiv - Neuroscience 2023Quote: ... freshly laid embryos were aligned against nitrocellulose paper and injected using a quartz capillary needle, three-axis micromanipulator (Narishige, MMO-4) and microinjector (Eppendorf FemtoJet 4X).
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bioRxiv - Genomics 2023Quote: ... Cells were pelleted by spinning at 800 g for 10 min at 4 °C in a swinging bucket centrifuge (Eppendorf Centrifuge 5810R). The supernatant was gently removed ...
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bioRxiv - Biophysics 2023Quote: ... This pre-gel solution was kept at 4 °C inside an amber UV-protected 1.5 mL microcentrifuge tube (Eppendorf®, Hauppauge, NY), and degassed for 1 h inside a vacuum chamber to reduce oxygen concentration and prevent early gelation inside the driving syringe while running the experiment ...
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bioRxiv - Immunology 2023Quote: ... cells were labeled with 50 nM of biotinylated human ACE2 protein (Acro Biosystems, AC2-H82E6) for 30 minutes at 4°C at 700 RPM on a shaker (Eppendorf, ThermoMixer C). The cells were subsequently washed ...
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bioRxiv - Microbiology 2023Quote: ... We performed fragmentation of 4 µg DNA in a 46-µL elution volume in Covaris G-tubes by centrifuging at 4200–5000 rpm (Eppendorf 5424 centrifuge) for 90 s to achieve fragment sizes of ∼20 kb ...
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bioRxiv - Systems Biology 2024Quote: ... with a mounted tissue section was dried to 37°C for 4 minutes on a thermal incubator (Eppendorf Thermomixer Option C, Germany) followed by in situ fixation in 4% PFA (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... To create extrachromosomal arrays constructs were microinjected into distal gonads of young adults using inverted microscope Leica DMi8 equipped with DIC filters and microinjection system InjectMan® 4 and FemtoJet® 4i (Eppendorf). Microinjection mixtures contained 10 ng/μL of the plasmid of interest ...
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bioRxiv - Microbiology 2024Quote: ... Pre-weighted metal-free 15 mL propylene tubes were used to pellet the cells in a tabletop centrifuge at 4 °C (Eppendorf, Hauppauge, NY). Pellets were washed three times with 10 mL of ice-cold PBS ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was centrifuged for 30 min at 15000 rpm at 4°C in the centrifuge Eppendorf 5702RH (Eppendorf SE, Hamburg, Germany). The pellet was resuspended in the 1/5 of the in initial volumes in PBS buffer (ThermoFischer Scientific ...
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bioRxiv - Developmental Biology 2024Quote: Chromatin was pre-cleared by centrifugation at 14,000×g for 15min at 4°C and the supernatant was moved to a 1.5mL DNA LoBind® tube (Eppendorf: Hamburg, Germany). From each chromatin sample per phenotype per lineage ...
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bioRxiv - Cell Biology 2024Quote: ... The sample was placed on ice to incubate for 30 minutes before the demembraned flagella supernatant was removed after centrifugation at 16,000 × g for 10 min at 4°C in a microfuge (Eppendorf, Centrifuge 5415 D). The pellet containing the axoneme was resuspended in 245 μl of Cilia Final Buffer (without trehalose) ...
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bioRxiv - Cell Biology 2020Quote: Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 5 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA ...
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bioRxiv - Immunology 2021Quote: ... Equal volume of Mycobacteria tuberculosis H37RA-CFA was mixed with MOG/PBS in a 5 mL protein Lobind tube (Eppendorf), followed by emulsification in ice water through sonication ...
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bioRxiv - Cell Biology 2019Quote: ... were detached with Trypsin-EDTA (0.25%) and pelleted by spinning them for 5 min at 1200 rpm (Eppendorf Centrifuge 5804). Cells were then re-suspended to 105 cells/mL in desiccation medium (50 mM Hepes and 500 mM trehalose in PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... and was injected at 1.5 µg/µl into the telencephalic vesicles of embryos in time-staged pregnant females using a Femtojet 4i microinjector (Eppendorf) then followed by electrical pulses (6 × 43 V ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 μL of 25 mM aqueous NH4HCO3 were added to each tube and incubated for 5 min at room temperature on a ThermoMixer (Eppendorf) at 700 rpm ...
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bioRxiv - Cancer Biology 2021Quote: ... All solution was removed and 100 μL of acetonitrile was added to each tube and incubated for 5 min at room temperature on a ThermoMixer (Eppendorf) at 700 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mg ml−1 lactic acid in water) and added to 5 mg of pre-washed TiO2 beads and incubated for 30 min on a Thermomixer (Eppendorf) at 1200 rpm to keep the beads in suspension ...
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bioRxiv - Bioengineering 2021Quote: ... a 96-well plate pre-filled with 20 μL pure water and sorted with designated cells per well was frozen on dry ice for 5 min and heated by ThermoMixer C (Eppendorf) at 95 °C for 10 min followed by spinning down at 3000 rpm for 1 min ...
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bioRxiv - Microbiology 2019Quote: ... The bacterial suspension was centrifuged at 5000 × g at room temperature for 5 minutes (Centrifuge 5430 R, Eppendorf AG, Germany). Then ...
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Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assemblybioRxiv - Cell Biology 2022Quote: ... The cells were kept at 37°C and 5% CO2 in a humidified incubator (Galaxy 170S CO2, Eppendorf, Hamburg, Germany) and regularly passaged at the confluence of 70-80%.
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bioRxiv - Cell Biology 2022Quote: ... The cells were kept at 37 °C and 5% CO2 in a Galaxy 170S CO2 humidified incubator (Eppendorf, Hamburg, Germany) and regularly passaged at the confluence of 70-80%.
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bioRxiv - Plant Biology 2019Quote: ... A volume of each protein extract corresponding to 16 mg protein was transferred into a new 5 ml LoBind tube (Eppendorf) containing Dynabeads MyOne Streptavidin C1 (Invitrogen ...
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bioRxiv - Immunology 2021Quote: RBD219-WT and RBD219-N1C1 were expressed at the 5 L scale using a Celligen 310 benchtop fermentation system (Eppendorf). For the RBD-WT ...
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bioRxiv - Cell Biology 2022Quote: All cells were kept at 37°C and 5% CO2 in a Galaxy 170 R humidified incubator (Eppendorf, Hamburg, Germany). They have also been regularly tested for mycoplasma contamination by examining the samples for extracellular DNA staining with SiR-DNA (100 nM ...
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bioRxiv - Genomics 2022Quote: ... 3 mM MgCl2 and 0.1% Tween 20 in water) and centrifuged for 5 min at 500 g in a swinging bucket rotor centrifuge (5920R, Eppendorf). Nuclei pellets were resuspended in 1X Tagmentation Buffer (10 mM Tris pH 7.4 ...
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bioRxiv - Plant Biology 2022Quote: ... The resulting pellet was re-suspended in 5 mL of modified CIB medium and centrifuged at 16,000 gav for 10 min in 5415R refrigerated centrifuge (Eppendorf, USA). The resulting supernatant was the Sucrose Gradient Load (SGL) ...
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bioRxiv - Neuroscience 2022Quote: ... and Sample Reducing Agent (10x) and incubated for 5 min at 70°C and 1100 rpm using a ThermoMixer comfort (Eppendorf).
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bioRxiv - Genomics 2022Quote: ... Eluted DNA was then passed through the column a second time and concentrated for hybridization to 5 μl with a SpeedVac Concentrator (Eppendorf).
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bioRxiv - Synthetic Biology 2024Quote: ... The cell suspension was then transferred to a pre-chilled electroporation cuvette and after approximately 5 – 10 min electroporated in the eporator electroporator (Eppendorf) set to 1850 V ...
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bioRxiv - Cell Biology 2024Quote: ... The culture was maintained in a humidified atmosphere containing 5% CO2 at 37 L in a cell incubator (Eppendorf, Germany). The medium was changed daily ...
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bioRxiv - Biophysics 2023Quote: ... at a final concentration of 5 mM at 37 °C for 40 minutes with shaking at 800 rpm in a thermomixer (Eppendorf). Alkylation was performed with iodoacetamide (IAA ...
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bioRxiv - Bioengineering 2023Quote: ... in LB (10 g Tryptone, 5 g Yeast Extract, 10 g NaCl) grown in a 10 L BioFlo 320 Fermenter (Eppendorf). At inoculation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We collected these bacteria into KK2 with a sterile loop and diluted them to 1.5 OD600 in KK2 (∼5 × 108 cells, measured with an Eppendorf BioPhotometer). We used these bacteria as food for amoebas in our experiment and repeated this process anew for each of the three replicate experiments.
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bioRxiv - Developmental Biology 2023Quote: ... were mixed in HEPES-CZB medium containing 5 μg/ml cytochalasin B (CB) and injected into the cytoplasm of fertilized eggs using a FemtoJet microinjector (Eppendorf) with constant flow settings ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We collected these bacteria into KK2 with a sterile loop and diluted them to 1.5 OD600 in KK2 (∼5 x 108 cells, measured with an Eppendorf BioPhotometer). We used these bacteria as food for amoebas in our experiment and repeated this process anew for each of the three replicate experiments.
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bioRxiv - Neuroscience 2023Quote: ... After centrifuging the samples for 15 minutes at 5°C at 4000 rpm on bench top centrifuge 5810R (Eppendorf, USA), 600 μL of dichloromethane (bottom layer ...
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bioRxiv - Cell Biology 2023Quote: ... After a 5-minute incubation the protein concentration was determined through measurement of the OD595 with a photometer (BioPhotometer plus, Eppendorf). Finally ...
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bioRxiv - Cell Biology 2024Quote: ... All cells were kept at 37 °C and 5% CO2 in a Galaxy 170 R humidified incubator (Eppendorf, Hamburg, Germany). All cell lines have been routinely examined for mycoplasma contamination by using SPY-555-DNA (1:100 000 ...
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bioRxiv - Microbiology 2024Quote: ... The culture was adjusted to OD600 75 by centrifuging 50 mL of culture in a conical tube at 3,220 ×g at room temperature for 5 min in a 5810 R centrifuge (Eppendorf, Germany). The supernatant was removed ...
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bioRxiv - Plant Biology 2019Quote: ... and proteins were concentrated using Amicon ultracentrifugal 30-kDa cut-off filters for 30 min at 4000 x g at 4°C (Eppendorf 5810 R centrifuge). The purified recombinant protein was stored in storage buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2019Quote: ... and proteins were concentrated using Amicon ultracentrifugal 30-kDa cut-off filters for 30 min at 4000 X g at 4°C (Eppendorf 5810 R centrifuge). The purified recombinant protein was stored in storage buffer (20 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2019Quote: ... The tubes were centrifuged at 18,000 x g and 4 °C for 15 min and the aqueous phase transferred to a 1.5 mL Eppendorf tube (Eppendorf UK Limited, Stevenage, UK). Two washes with 500 µl 24:1 chloroform/isoamyl alcohol were performed ...
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bioRxiv - Microbiology 2021Quote: ... The media was spun down in a centrifuge at 4°C for 10 min at 4400 g (Centrifuge 5920 R, Eppendorf Nordic, Hørsholm, Denmark) and the supernatant collected and stored at -80° for 1H NMR analysis ...
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bioRxiv - Microbiology 2021Quote: ... Development of ∼60 % cytopathic effect (CPE) led to harvesting and clarification of supernatants (3184 x g, 20 min, 4 °C, in an Eppendorf 5810 R centrifuge). Aliquots were then titred by plaque assay (see below) ...