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Citations for Eppendorf :
51 - 77 of 77 citations for L Asparagine H2O 13C4; D3; 15N2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... after which 25 μL of the eluate was taken out and transferred to a clean new 1.5 mL DNA Lobind tube (Eppendorf, DE) (5 μL were left behind) ...
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bioRxiv - Cell Biology 2024Quote: ... The culture was maintained in a humidified atmosphere containing 5% CO2 at 37 L in a cell incubator (Eppendorf, Germany). The medium was changed daily ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 6 μL by 0.1% TFA.
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). Peptide samples were then prepared for LC-MS/MS analysis by dilution to 5 μL using 0.1% TFA.
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bioRxiv - Cell Biology 2023Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL with 0.1% TFA.
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Phosphoproteomics of cellular mechanosensing reveals NFATC4 as a regulator of myofibroblast activitybioRxiv - Systems Biology 2023Quote: ... Bound phosphopeptides were eluted 2 times with 30 μl elution buffer (40 % ACN, 15 % NH4OH (25 %, HPLC grade)) each and collected by centrifugation into clean PCR tubes (Eppendorf). Samples were concentrated in a SpeedVac for 15 min ...
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bioRxiv - Bioengineering 2023Quote: ... in LB (10 g Tryptone, 5 g Yeast Extract, 10 g NaCl) grown in a 10 L BioFlo 320 Fermenter (Eppendorf). At inoculation ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). They were then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA.
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bioRxiv - Cell Biology 2023Quote: ... and eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). They were then prepared for LC-MS3 analysis by diluting it to 5 μL by 0.1% TFA ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μL of the reaction mixture were added to 30 μL BCA reagent solution in a 384-well PCR plate (Eppendorf twin.tec PCR MTP 384 ...
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bioRxiv - Microbiology 2020Quote: Up-scale cultivation of the PmamDC45-trans-at-pks strain was done in a 10 L BioFlow® 120 Fermenter (Eppendorf) (34 ...
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bioRxiv - Genomics 2022Quote: ... 70μl of the released material from each well were collected and stored in a new 96-well PCR plate (Eppendorf, Germany). Library preparation ...
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bioRxiv - Cell Biology 2023Quote: ... Protein pellets were dissolved in 0.1% RapiGest and 500 mmol/L TEAB and then incubated at 850 rpm for 45 min at 37 °C (Eppendorf Comfort Thermomixer, Eppendorf, USA). Proteins were digested by trypsin (Trypsin Gold ...
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bioRxiv - Plant Biology 2023Quote: ... The beads were then washed with 3 x 100µL of the lactic acid binding solution and finally resuspended in 50µL and placed into 200 µL tips (Eppendorf, Hauppauge, NY) plugged with 2 layers of 3M™ C8 Empore™ membrane (ThermoFisher Scientific ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... at 0.2 g/L in E3 medium and injected with molecules diluted in PBS using a microinjector (FemtoJet, Eppendorf, Montesson, France) under a stereomicroscope ...
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bioRxiv - Biophysics 2023Quote: ... were coated with Expi293 cell produced RS2 at 4 µg/mL concentration in 1x PBS (60 µl/well) and incubated for 2 h at 25 °C under gentle shaking condition (300 rpm) on a thermomixer (Eppendorf, USA) and then plate was transferred to 4 °C cold room for overnight ...
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bioRxiv - Cell Biology 2024Quote: ... and 800 µM or 3.2 mM L-cystine at 37 °C and 500 rpm using an Eppendorf ThermoMixer® (Eppendorf, Germany). Incubation times varied according to the reagent ...
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bioRxiv - Bioengineering 2024Quote: ... The 12 initial cultivations were executed in a DASGIP© Parallel Bioreactor System (max. working volume 2 L; Eppendorf, Hamburg, Germany) as described in [51] ...
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bioRxiv - Microbiology 2021Quote: ... 10 µL was transferred to a PCR-clean polypropylene V bottom 96-well plate and covered with a silicone seal (Eppendorf, Mississauga, ON). Reactions were incubated at 29°C for 16 hours and final reactions stored at -20°C ...
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bioRxiv - Biochemistry 2020Quote: ... a 250 mL preculture was used to inoculate 3.75 L of 70% D2O minimal media in a BioFlo 310 bioreactor (Eppendorf, Hauppauge, NY, USA). Cells were grown at 37 °C with dry ...
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bioRxiv - Molecular Biology 2022Quote: ... 2.5 µL of 100 mM Dithiothreitol (DTT) was added to each sample and incubated at 60LJ C for 15 mins (Eppendorf, Thermomixer C) and alkylated using 2.5 µL of 200 mM iodoacetamide (IAM ...
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bioRxiv - Bioengineering 2024Quote: Coating formulations were prepared by dissolving the lyophilized protein powder in MilliQ water to the final concentration of 0.01 - 10 g/L and then centrifuged for 10 min at 10000 rpm (Eppendorf Centrifuge 5430 R). The supernatant was taken for surface coating.
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bioRxiv - Biochemistry 2024Quote: ... four times (1600 μL) ice-cold 80% acetone was added to 400 μL of urine in 2 ml protein Lo-Bind tubes (Eppendorf, Hamburg, Germany). After incubating at −20 °C for 1hr ...
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bioRxiv - Microbiology 2024Quote: ... and 40 mL of the supernatant was added as the inoculum to 5 L medium prepared in a BioFlo® 120 bioreactor (Eppendorf, Hamburg, Germany). Air containing 0.5% or 10% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... Protein pellets were dissolved in 0.1% RapiGest and 500 mmol/L TEAB and then incubated at 850 rpm for 45 min at 37 °C (Eppendorf Comfort Thermomixer, Eppendorf, USA). Proteins were digested by trypsin (Trypsin Gold ...
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bioRxiv - Biochemistry 2024Quote: ... the samples received 50 µl 1 M NaOH (500 mM final concentration; dispensed with an insulin syringe by punching the lid of the Eppendorf, without opening the tube).