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Citations for Eppendorf :
1 - 50 of 77 citations for L Asparagine H2O 13C4; D3; 15N2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... 80 µL H2O) for 6 minutes on a shaker (Eppendorf Thermomixer Comfort) at 28°C and 400 rpm ...
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bioRxiv - Plant Biology 2021Quote: ... were made from single colonies of putative Xcm strains and cell suspensions (optical density (OD) = 0.3) were generated in MilliQ ultrapure H2O using a spectrophotometer (Eppendorf BioSpectrometer basic ...
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bioRxiv - Bioengineering 2023Quote: ... coli JM108 were grown in a 1.8 L (1.0 L net volume, 0.5 L batch volume) computercontrolled bioreactor (DASGIP parallel bioreactor system, Eppendorf AG, Germany). The bioreactor was equipped with a pH probe and an optical dissolved oxygen probe (Hamilton Bonaduz AG ...
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bioRxiv - Cell Biology 2021Quote: Lipids were extracted from 100 mg of ground germlings overnight at 4 °C with 4.5 mL of methanol (MeOH): water (H2O) (25:75 by v/v) at 750 rpm (revolution per minute) in a thermomixer (Eppendorf, Hamburg, Germany). Lipids from 100 µl of conditioned media were extracted by vortexing for 2 min with 4.5 mL of methanol (MeOH) ...
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bioRxiv - Immunology 2024Quote: ... stool suspensions of feces (in H2O) were centrifuged at 16000 rpm and 4°C for 5 min using an Eppendorf 5427R centrifuge (Eppendorf; Hamburg, Germany). 100 µL of supernatant were mixed with 400 µL of ice-cold methanol containing recovery standards for evaluation of the quality of cell harvest and correction for variations ...
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bioRxiv - Microbiology 2020Quote: ... 10 g NaCl) in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf) were used to express the I53-50A or I53-50B.4PT1 proteins grown ...
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bioRxiv - Immunology 2024Quote: ... 10 g NaCl) in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf), were transformed with a I53-50B.4PT1-encoding plasmid ...
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bioRxiv - Immunology 2022Quote: ... 10 g NaCl) grown in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf). Cells were grown at 37°C to an OD600 ∼ 0.8 ...
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bioRxiv - Immunology 2020Quote: ... 10 g NaCl) grown in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf). Cells were grown at 37°C to an OD600 ~0.8 ...
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bioRxiv - Cell Biology 2021Quote: ... 20 g/L tetracycline hydrochloride) was grown in batch mode in a 1 L fermenter (DASGIP 1L Bioreactor, Vaudaux Eppendorf) at pH 7.0 (+/-0.2 ...
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bioRxiv - Microbiology 2020Quote: ... 20 mL of the enrichment was transferred to 5 L NMS medium in a 6-L fermenter controlled with a BioFlo® 120 Bioprocess Control Station (Eppendorf, Hamburg, Germany). Gas stream carrying a relatively low CH4 concentration (0.5% v/v in air ...
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bioRxiv - Microbiology 2023Quote: Bioreactor cultivations were performed using 1.0 L DASGIP reactors (Eppendorf). Fermentations were performed using YNB with 10 g L−1 (NH4)2SO4 as the nitrogen source ...
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bioRxiv - Synthetic Biology 2020Quote: Bioreactor cultivations were performed in 1.3-L fermenters (BioFlow120, Eppendorf, Germany) with a working volume of 0.5 L mineral salt medium (0.4 L mineral salt medium in fed-batch) ...
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bioRxiv - Synthetic Biology 2020Quote: Batch fermentations were conducted in 1 L DASGIP bioreactors (Eppendorf, Germany) with an initial volume of 550 mL ...
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bioRxiv - Molecular Biology 2021Quote: Protein expression was scaled-up in a 1.3 L bioreactor (Eppendorf, USA) with 0.5 L initial volume ...
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bioRxiv - Microbiology 2022Quote: ... viennensis was grown as a continuous culture in 2 L bioreactors (Eppendorf) filled with 1.5 L of fresh water medium (FWM ...
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bioRxiv - Synthetic Biology 2023Quote: Fed-batch fermentations were conducted using a 6.6 L bioreactor (BioFlo 320, Eppendorf) containing 1.7 L R/2 medium (pH 6.8 ...
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bioRxiv - Synthetic Biology 2020Quote: ... We then set up a BioFlo120 system with a 5 L bioreactor (Eppendorf, B120110002) and added 3 L of SC-His medium supplemented with 15% glucose after autoclaving ...
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bioRxiv - Systems Biology 2023Quote: ... Cultivations were carried out in DasGip 1-L stirrer-pro vessels (Eppendorf, Jülich, Germany). The working volume was 500 mL ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were seeded on poly-L-lysine pretreated (0.001%, 1h) 24-well imaging plates (Eppendorf, Germany) at a density of 1e05 cells/well ...
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bioRxiv - Plant Biology 2022Quote: ... Fermentation was conducted in approximately 3 L of culture in a bioreactor BioFlo120 (Eppendorf, Hamburg, Germany) at 30°C for 5 days ...
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bioRxiv - Microbiology 2023Quote: ... intermedia CBS 141442 was grown in controlled stirred 1-L bioreactor vessels (DASGIP, Eppendorf, Hamburg, Germany) containing 500 mL synthetic defined minimal Verduyn media with 2% Glucose ...
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bioRxiv - Bioengineering 2024Quote: ... intermedia CBS 141442 was grown in controlled stirred 1-L bioreactor vessels (DASGIP, Eppendorf, Hamburg, Germany) containing 500 mL minimal Verduyn media with 2% Glucose ...
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bioRxiv - Bioengineering 2023Quote: Chemostat cultivations were performed at 28°C in DasGip systems with 1 L stirrer -pro vessels (Eppendorf) as previously reported63 ...
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bioRxiv - Immunology 2020Quote: ... and fermentation was conducted in a CelliGen 310 Bioreactor with a 7.5 L vessel (Eppendorf, New York, USA), controlled by the Eppendorf Bio Command software ...
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bioRxiv - Biochemistry 2022Quote: ... 15 L of T-20052 (53) medium was prepared in a 20-liter Bioflow IV bioreactor (Eppendorf/NBS). The seed culture was collected and centrifuged at 3,000 x g for 10 minutes at 25¼;C ...
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bioRxiv - Bioengineering 2023Quote: Bioreactor fermentations at bench-top level were performed in DasGip 1-L stirrer-pro vessels (Eppendorf, Jülich, Germany) at 30 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 5μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA ...
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bioRxiv - Synthetic Biology 2021Quote: ... 40-120 μl of culture supernatant was dried in 1.5 ml microcentrifuge tubes with a rotational vacuum concentrator (Concentrator Plus, Eppendorf) and derivatised in 20 μl methoxyamine chloride (30 mg/ml in pyridine ...
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bioRxiv - Plant Biology 2021Quote: Seeds of tobacco (Nicotiana tabacum L. cv. SR1) or Arabidopsis Colombia were placed in a 1.7 mL micro-tube (Eppendorf) filled with sodium hypochlorite (0.5% active material ...
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bioRxiv - Neuroscience 2020Quote: ... washed twice with 160 µL PBS and nearly 40,000 cells per well transferred onto the membrane and dried with a plate thermomixer (Eppendorf) at 50 °C ...
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bioRxiv - Immunology 2020Quote: ... Process development was performed in a scale-down models in a 10 L glass-vessel bioreactor (BioFlo 320, Eppendorf). Established process parameters were then scaled up to 120 L in a BioFlo Pro 150 stainless steel fermenter (Eppendorf) ...
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bioRxiv - Immunology 2020Quote: ... Established process parameters were then scaled up to 120 L in a BioFlo Pro 150 stainless steel fermenter (Eppendorf). Phosphate depletion during the growth phase induced UF6b expression into inclusion bodies ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were eluted in 40μl of 80% acetonitrile in 0.1% TFA and concentrated down to 1μl by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5μl by 0.1% TFA.
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bioRxiv - Molecular Biology 2023Quote: ... 66 Peptides were eluted in 40 μl of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μl by vacuum centrifugation (Concentrator 5301, Eppendorf). They were then prepared for LC-MS/MS analysis by diluting it to 5 μl by 0.1% TFA.
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were eluted with freshly prepared 60 μl of 1.25 % v/v ammonium hydroxide (NH4OH)/80 % v/v ACN and dried using a SpeedVac centrifuge (Eppendorf). Dried peptides were resuspended in 6 μL buffer A (2 % v/v ACN/0.1 % v/v TFA) ...
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bioRxiv - Molecular Biology 2024Quote: ... peptides were eluted in 40 μl of 80% acetonitrile in 0.1% TFA and concentrated down to 2 μl by vacuum centrifugation (Concentrator 5301, Eppendorf). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μl by 0.1% TFA ...
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bioRxiv - Biophysics 2024Quote: ... Larvae of 3 dpf were mounted in 2.5 mm wide V-shaped agarose trenches and microinjected after 160 mg/L tricaine treatment using a mechanical micromanipulator (CellTram oil vario microinjector, 5176000.025, Eppendorf) with GBM cells expressing GFP ...
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bioRxiv - Cell Biology 2020Quote: ... The whole lane was excised from the gel and future cut into approximate 3 x 1 x 2 mm3 slices (L x W x H) and each slice were placed into a separate LoBind tubes (Eppendorf) for destaining ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were eluted in 40 μl of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μl by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). Samples were then prepared for LC-MS/MS analysis by diluting them to 5 μl with 0.1% TFA ...
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bioRxiv - Cell Biology 2020Quote: Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 5 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA ...
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bioRxiv - Bioengineering 2021Quote: ... cerevisiae CEN.PK-113-7D were performed in a 1 l working volume of a stirred fermenter (DASGIP parallel bioreactor system, Eppendorf). Throughout the fermentations the pH was kept at 5.1 and the temperature was kept at 30 degrees Celsius ...
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bioRxiv - Cancer Biology 2021Quote: ... All solution was removed and 100 μL of acetonitrile was added to each tube and incubated for 5 min at room temperature on a ThermoMixer (Eppendorf) at 700 rpm ...
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bioRxiv - Bioengineering 2021Quote: ... and 100μL of pre-warmed Mammocult was added to the center of each well using an epMotion 96 liquid handler (Eppendorf). To generate larger rings (maxi-rings ...
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bioRxiv - Systems Biology 2020Quote: ... A fixed volume of 100 μL cells was used to inoculate 50 mL preculture M9 +40 mM MOPS media with 4 g/L glucose which was grown overnight in a shake flask at 200 rpm in a 37°C (Eppendorf) incubator ...
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bioRxiv - Microbiology 2020Quote: ... Newly emerged EVV-1 free EvaBx adults were CO2 anaesthetized and injected with 0.5 μL of the virus-containing extract between two abdominal segments using a stereomicroscope with a Cell Tram Oil microinjector (Eppendorf). As negative control in order to exclude any procedural contamination ...
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bioRxiv - Immunology 2021Quote: RBD219-WT and RBD219-N1C1 were expressed at the 5 L scale using a Celligen 310 benchtop fermentation system (Eppendorf). For the RBD-WT ...
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bioRxiv - Microbiology 2022Quote: ... 500 ul of fixative containing cells were added on 12 mm glass coverslip kept on a 35mm petri plate (glass coverslips were precoated with Ploy-L-Lysine) and centrifuged in a swing bucket centrifuge (Eppendorf) for 10 min at 5000 g ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL with 0.1% TFA.
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM D-L methionine) for 1 h at 4° C with shaking at 1400 rpm in a benchtop thermomixer 22331(Eppendorf). The beads were washed twice with binding buffer without soybean trypsin inhibitor and suspended in 50 % v/v binding buffer ...