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Citations for Eppendorf :
51 - 100 of 107 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... lipid extracts were dissolved in 10 mM ammonium acetate in methanol and transferred to 96-well plates (Eppendorf twintec plate 96). Mass spectrometric measurements were performed in positive ion mode on an AB SCIEX QTRAP 6500+ mass spectrometer ...
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bioRxiv - Systems Biology 2020Quote: ... Peptides were eluted with 60 μl of 1.25% Ammonium hydroxide (NH4OH)/ 80% ACN and dried using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Dried peptides were resuspended in buffer A* (2% ACN/ 0.1% TFA ...
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bioRxiv - Systems Biology 2020Quote: ... Peptides were eluted with 80 µl of 1.25% Ammonium hydroxide (NH4OH)/ 80% ACN and dried using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Samples were resuspended in buffer A* (0.2% TFA/ 2% ACN ...
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bioRxiv - Systems Biology 2021Quote: ... Peptides were eluted with 75 µl of 1.25% Ammonium hydroxide (NH4OH) in 80% ACN and dried using a SpeedVac centrifuge (Eppendorf, Concentrator plus). They were resuspended in buffer A* (0.2% TFA/ 2% ACN ...
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bioRxiv - Cell Biology 2020Quote: ... Captured peptides were eluted with 60 µl of 1.25% Ammonium hydroxide(NH4OH)/80% ACN and dried using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Dried peptides were resuspended in buffer A* (2 % ACN/0.1 % TFA).
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bioRxiv - Physiology 2024Quote: Protein samples were dissolved in 8 M urea in 50 mM ammonium bicarbonate (pH 7.8) by vortexing for 1 hour (Eppendorf ThermoMixer F1.5). 5 mM dithiothreitol (DTT ...
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bioRxiv - Cell Biology 2022Quote: ... Log phase yeast were transferred to concanavalin A-treated 96-well glass bottom plates (Eppendorf, Hamburg, Germany) and imaged using a DMi8 microscope (Leica Microsystems ...
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bioRxiv - Systems Biology 2022Quote: ... The polar and the non-polar phase were transferred into two new and separate reaction vials (Eppendorf low binding tube ...
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bioRxiv - Biophysics 2022Quote: ... the FG phase (insoluble content) was pelleted by centrifugation (21130g, 30 mins, using a temperature-controlled Eppendorf 5424 R centrifuge equipped with a FA-45-24-11 rotor ...
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bioRxiv - Biophysics 2022Quote: ... the FG phase (insoluble content) was pelleted by centrifugation (21130g, 30 mins, using a temperature- controlled Eppendorf 5424 R centrifuge equipped with a FA-45-24-11 rotor ...
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bioRxiv - Cell Biology 2022Quote: ... using Femtotip II microinjection capillaries (Cat#. 5242957.000, Eppendorf) combined with the Eppendorf electronic microinjector FemtoJet 4x (Cat# ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was loaded in Femtotips II (Eppendorf) and injection was done with an injection pressure of 1.0 hPa ...
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bioRxiv - Microbiology 2022Quote: ... Two-microliter aliquots of resuspended lipids were diluted 1:10 in 10 mM ammonium acetate in methanol in 96-well plates (Eppendorf twin tec 96) prior to measurement ...
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bioRxiv - Microbiology 2022Quote: ... Two μl aliquots of the resuspended lipids were diluted 1:10 in 10 mM ammonium acetate in methanol in 96-well plates (Eppendorf twin tec 96) prior to measurement ...
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bioRxiv - Cancer Biology 2021Quote: ... The aqueous (top) phase was removed by aspiration and transferred to a clean 1.5 mL tube (Eppendorf, Germany). A volume of 100% RNAse-free ethanol (MilliporeSigma ...
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bioRxiv - Developmental Biology 2023Quote: ... The lower phase was transferred into a fresh tube and dried in a speed-vac (Eppendorf concentrator plus) for 15 min at 45°C and redissolved in 500 µl spray buffer (8/5/1 isopropanol/MeOH/H2O (all LC-MS grade ...
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bioRxiv - Microbiology 2024Quote: ... with solutions injected through Femtotip II glass microcapillaries (Eppendorf), and the microcapillary position controlled with a MP-285 micromanipulator (Sutter ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:1v/v mixture of 33% ammonium hydroxide and 40% methylamine in water for 3 h at 37 °C (Eppendorf ThermoMixer C, 1000 rpm). The suspension was filtered ...
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bioRxiv - Neuroscience 2022Quote: ... Proteolytic peptides were eluted from the solid-phase extraction material and dried out completely in a SpeedVac centrifuge (Eppendorf). Finally ...
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bioRxiv - Systems Biology 2023Quote: ... The upper hydrophilic phase was dried overnight using a speed vacuum centrifuge (Eppendorf concentrator plus, Eppendorf AG, Hamburg, Germany). The dried samples were rehydrated by adding 300 μl of D2O and 400 μl of a 0.09 M phosphate buffer solution (pH=7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... The aqueous phase was combined with the first supernatant and was dried overnight in Speed-Vac (Eppendorf Concentrator plusTM). The metabolites were quantified using multiple reaction monitoring (MRM ...
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bioRxiv - Biophysics 2021Quote: ... Lipid extracts were resuspended in 60 μl 10mM ammonium acetate in methanol and diluted 1:4 in 96-well plates (Eppendorf twin.tec® 96; Sigma-Aldrich) prior to measurement of PC species ...
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bioRxiv - Developmental Biology 2023Quote: ... microinjector with Femtotip II microinjection capillary tips (Eppendorf, Cat# 930000043) at 100 hPa pressure for 0.2 seconds ...
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bioRxiv - Microbiology 2020Quote: ... This mixture was centrifuged at maximum speed for 1 min and the supernatant was transferred to a tube containing 300 μL chloroform and Heavy Phase Lock Gel (Eppendorf), inverted for 2 min and centrifuged at maximum speed for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... The upper polar phase was transferred completely into a new microtube and subsequently dried by vacuum concentration (Concentrator plus, Eppendorf). The dried extracts were re-suspended in 200 µL deionized water and filtrated (0.2 µm filters ...
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bioRxiv - Molecular Biology 2021Quote: ... Unincorporated biotin-HPDP was removed by two rounds of chloroform extraction with 2 ml Phase lock gel heavy tubes (Eppendorf). RNA was precipitated with 1/10 volume of 5M NaCl and an equal volume of Isopropanol ...
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bioRxiv - Cell Biology 2023Quote: ... and 450 μl of the aqueous phase (upper layer) was transferred to a new RNase-free centrifuge tube (Biopur, Eppendorf), followed by mixing with 450 μl of isopropanol ...
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bioRxiv - Physiology 2023Quote: ... and 450 μl of the aqueous phase (upper layer) was transferred to a new RNase-free centrifuge tube (Biopur, Eppendorf), followed by mixing with 450 μl of isopropanol ...
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bioRxiv - Molecular Biology 2024Quote: ... After The upper lipophilic phases and lower hydrophilic phases were separated by high-speed centrifugation (12,700 rpm, 5 min at 4 °C, Centrifuge 5430R, Eppendorf, Germany). Next ...
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bioRxiv - Microbiology 2024Quote: ... Biomass samples were collected in mid-exponential phase by centrifuging (3214 x g; Eppendorf 5810 R, S-4-104 rotor) 50 mL of culture for 15 minutes at 4 °C ...
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bioRxiv - Genomics 2022Quote: ... The 325 µl of the aqueous phase from the chloroform extraction was then transferred to a new 1.5 ml centrifuge tube (Eppendorf 0030 125.215). 650 µl of 100% ethanol and 33 µl of 3 M sodium acetate pH 5.2 (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... 1.5 mL of overnight cultures in LB media in the stationary phase of growth were added to 2mL microcentrifuge tubes (Eppendorf, Canada) and incubated in a 60°C water bath (model FSGPD28 ...
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bioRxiv - Neuroscience 2020Quote: ... Tubes were centrifuged at 14k rpm for 15 minutes at 4°C and the aqueous phase was collected into clean 1.5 mL Eppendorf tubes (Eppendorf, Hauppauge, NY). 500 µL of ice-cold isopropanol was added to each tube ...
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bioRxiv - Systems Biology 2022Quote: ... The polar and the non-polar phase were transferred into two new and separate reaction vials (Eppendorf low binding tube, 1.5 mL, Eppendorf, Hamburg, Germany), evaporated to dryness using an Eppendorf Concentrator Plus (Eppendorf ...
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bioRxiv - Plant Biology 2024Quote: ... and aliquots of 150μL of the upper phase of each sample were transferred to new 1.5mL tubes and dried overnight by Speedvac (Concentrator Plus, Eppendorf, Hamburg, Germany). Derivatization was carried out as previously described (58).
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bioRxiv - Bioengineering 2021Quote: ... with microinjection capillaries (Femtotip II, inner diameter of 500 nm, Eppendorf). For injection ...
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bioRxiv - Biochemistry 2021Quote: ... Resulting peptides were desalted by reverse-phase purification using a SepPak 96-well plate and dried by vacuum centrifugation (Concentrator plus, Eppendorf, Hamburg, Germany).
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bioRxiv - Microbiology 2020Quote: ... we centrifuged a 0.5-mL sample of culture in the exponential phase at 7000 rpm for 5 min in a Benchtop centrifuge (5424 Eppendorf, Hamburg, Germany). We washed the pelleted biomass 1 – 2 times and resuspended it with 50 µL 1x PBS from which we fixed 2 µL on solidified agarose (VWR ...
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bioRxiv - Neuroscience 2021Quote: ... This was repeated and the collected organic phases containing the retinoids were dried using the SpeedVac system (Vacufuge Plus, Eppendorf, Hamburg, Germany). The dried samples were reconstituted in hexanes and used for HPLC separation ...
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bioRxiv - Cancer Biology 2023Quote: ... and the upper phase (water-soluble metabolites) was evaporated for 30 minutes at 35°C under nitrogen and dried in SpeedVac (Eppendorf, Hamburg, Germany) at 15 °C overnight ...
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bioRxiv - Microbiology 2024Quote: ... castellanii trophozoites were seeded at the log growth phase (1 × 104 cells per well) in a 96-well white microplate (Eppendorf, Hamburg, Germany). Incubation with medium containing varied nitroxoline concentrations ranging from 0 to 100 μM was performed ...
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bioRxiv - Biophysics 2021Quote: ... We put 500 mL of log phase density (1×106-1×107 cells/mL) cells into 1 mL disposable cuvettes (UVette, 952010051, Eppendorf AG, Hamburg, Germany). We measured the OD600 every hour for 7 hours in each of two cuvettes for each cell line ...
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bioRxiv - Developmental Biology 2021Quote: Preparation for microinjection was carried out with a Femtotips II microinjection needle (Eppendorf) and a gas pressure injection system were used to inject oil into the Stage 14 egg chambers [31] ...
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bioRxiv - Biophysics 2024Quote: ... and microinjected into the cytoplasm with a glass capillary needle (Femtotips II; Eppendorf). The volume of injected solution was estimated to be 2 fL.
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bioRxiv - Biochemistry 2020Quote: ... polar phase were transferred into a new 2 mL reaction tube and dried in a vacuum concentrator (Eppendorf, Concentrator plus, mode: V-AQ, 30 °C) for approximately 4.5 h ...
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bioRxiv - Molecular Biology 2024Quote: ... The upper solvent partition phase (methanol/water) was transferred to a new micro-centrifuge tube and then dried out in a vacuum concentrator (Eppendorf Concentrator plus™, Eppendorf; USA) at 200 °C for 2 h followed by 12 h freeze drying in a lyophilizer ...
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bioRxiv - Cell Biology 2021Quote: ... Microinjections utilized an Eppendorf Femtojet apparatus with Femtotips II pre-pulled glass needles (Eppendorf). All Drosophila melanogaster strains and crosses were maintained on standard medium at 25 °C unless stated otherwise ...
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bioRxiv - Cell Biology 2023Quote: ... with Femtotips II at a transmitted light microscope or using a FemtoJet microinjector (Eppendorf) mounted on an inverted Leica SP8 confocal microscope.
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bioRxiv - Molecular Biology 2024Quote: ... Under a binocular microscope and using microcapillaries pipettes (FemtoTip II ®, Eppendorf, Montesson, France), 10 μL DNA (3 μg μL- 1 - 0.05 % FG ...
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bioRxiv - Molecular Biology 2020Quote: ... Eppendorf FemtoJet® 4x programmable microinjector and Eppendorf Femtotip II injection capillaries (Eppendorf, Hamburg, Germany). The injection conditions were injection pressure (pi ...