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Citations for Eppendorf :
1 - 50 of 107 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The upper phase was carefully removed and transferred to a 50 mL phase-lock gel tube (Eppendorf), and 10 mL of 24:1 chloroform:isoamyl alcohol was added and gently mixed by inversion for 1 minute ...
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bioRxiv - Biochemistry 2022Quote: ... A total of 700 µl of the upper phase was recovered into a phase-lock (heavy) tubes (Eppendorf) and a second phenolic treatment was performed by the addition of 700 µl of acidic phenol-chloroform and mixing thoroughly by inverting the tubes ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl of the lipid-containing lower organic phase was spotted on a MALDI target plate (Prespotted AnchorChip 96 Set for Proteomics II; Bruker Daltonics/Eppendorf; washed peptide-free with 2-propanol) pre-spotted with 1 µl of 9-aminoacridine (10 mg/ml dissolved in acetone:water 9:1 (v/v) ...
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bioRxiv - Biophysics 2022Quote: ... the DNA droplet-containing aqueous phase was layered on top of the oil phase in a volumetric ratio of 1:3 within a microtube (Eppendorf). For the segregation of DNA droplets using enzymatic activity ...
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bioRxiv - Biophysics 2020Quote: ... the DNA-containing aqueous phase was layered on top of the oil phase in a volumetric ratio of 1:3 within a microtube (Eppendorf). Droplet formation was induced by manual shaking for about 4 s as described earlier.[26] For the oil-phase ...
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bioRxiv - Neuroscience 2022Quote: ... Phase-lock heavy gel (Eppendorf AG, Hamburg, Germany), and RNeasy mini spin columns (Qiagen ...
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bioRxiv - Plant Biology 2022Quote: ... organic and aqueous phases were separated by centrifugation (Eppendorf). The whole procedure yielded eight fractions ...
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bioRxiv - Genetics 2020Quote: ... RNA was transferred to Phase Lock Gel Heavy Tubes (Eppendorf), and an equal volume of chloroform was added ...
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bioRxiv - Synthetic Biology 2020Quote: ... Organic phases were removed to new tubes and dried in vacufuge (Eppendorf). For Δare1Δare2 double mutants ...
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bioRxiv - Molecular Biology 2022Quote: ... harvested at log phase (OD600 = 0.8–1.0; measured on an Eppendorf BioPhotometer) and mixed in a 9:1 ratio ...
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bioRxiv - Biochemistry 2024Quote: All the protein extracts were resuspended in 100 mM ammonium bicarbonate in a Lobind microcentrifuge tube (Eppendorf), dried by lyophilization ...
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bioRxiv - Neuroscience 2020Quote: ... All phase-separated droplets were imaged on a 96 glass well plate (Eppendorf). Two-or three-fold concentrated protein or RNA samples were prepared to account for the dilution in mixing with other components to achieve desired final concentrations ...
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bioRxiv - Systems Biology 2023Quote: ... The upper hydrophilic phase was dried overnight using a speed vacuum centrifuge (Eppendorf concentrator plus ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 75] The organic phase was evaporated using an Eppendorf Concentrator Plus System (Eppendorf, Hamburg ...
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bioRxiv - Microbiology 2024Quote: ... 500 μl of the aqueous phase was transferred to DNA LoBind tubes (Eppendorf) to which 750 μl of absolute ethanol was added ...
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bioRxiv - Plant Biology 2021Quote: ... and VacuTip II microcapillaries (Eppendorf) were used to isolate about 40-50 washed embryos in 50 μl isolation buffer.
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bioRxiv - Cell Biology 2021Quote: ... with Femto tips II (Eppendorf) into the perivitelline space on the ventral side of Stage 10 embryos ...
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bioRxiv - Cell Biology 2022Quote: ... Lipids were resuspended in 10 mM methanolic ammonium acetate and transferred to 96-well plates (Eppendorf Twintec 96). Mass spectrometry was performed on a Sciex QTRAP 6500+ mass spectrometer ...
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bioRxiv - Biophysics 2020Quote: ... The needle was then glued to a 6×4×2 mm Neodyn magnet (QM-06×04×02-N, Magnets4you) and attached to a motorized micromanipulator (PatchMan, Eppendorf). The magnetic needle was lowered until it touched the bottom of a dummy sample dish and raised again so that it was placed above the bottom of the sample with the very tip in focus around 100 μm above the focus of the glass surface.
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bioRxiv - Microbiology 2020Quote: ... The aqueous layer was transferred into a 2 ml phase lock gel tube (Eppendorf), 750 μl chloroform (Roth ...
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bioRxiv - Genomics 2021Quote: ... The aqueous phase was transferred to a low-binding eppendorf tube (Eppendorf, Cat #022431021) and then 40µl of Sodium Acetate (3M) ...
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bioRxiv - Molecular Biology 2022Quote: ... The aqueous layer was transferred into a 2 ml phase lock gel tube (Eppendorf) and mixed with 750 μl chloroform (Roth ...
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bioRxiv - Biochemistry 2023Quote: ... Upper organic phase with collected and subsequently dried in vacuo (Eppendorf concentrator 5301, 1ppm).
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bioRxiv - Genomics 2024Quote: ... The aqueous phase was transferred to a low-bind Eppendorf tube (Eppendorf, Cat #022431021). 40µl of Sodium Acetate (3M) ...
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bioRxiv - Biochemistry 2024Quote: ... The organic phase was evaporated to dryness using an Eppendorf Concentrator Plus System (Eppendorf, 5305000509 ...
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bioRxiv - Biochemistry 2024Quote: ... the upper aqueous phase was transferred into a new 1.5 mL RNAsefree tube (Eppendorf) with 100 µL of 7.5 M LiCl and a 1% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were eluted with 80 μl of 1.25% Ammonium hydroxide (NH4OH)/80% ACN and dried using a SpeedVac centrifuge (Concentrator Plus; Eppendorf). MS loading buffer (0.2% TFA/2%ACN (v/v) ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were eluted with freshly prepared 60 μl of 1.25 % v/v ammonium hydroxide (NH4OH)/80 % v/v ACN and dried using a SpeedVac centrifuge (Eppendorf). Dried peptides were resuspended in 6 μL buffer A (2 % v/v ACN/0.1 % v/v TFA) ...
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bioRxiv - Plant Biology 2023Quote: ... Bound phosphopeptides were then eluted 3 times with 100 µL ammonium hydroxide (5% v/v) into 1.5mL Lo-Bind tubes (Eppendorf). These were then frozen and lyophilized ...
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bioRxiv - Systems Biology 2023Quote: The dried protein pellet was resuspended in in 30 µL of 8 M Urea dissolved in 100 mM Ammonium bicarbonate (ABC) (Fluka) pH 8.8 and incubated at 1000 rpm at 25 °C on a thermoshaker (Eppendorf). Urea concentration was lowered to 0.8 M by addition of 250 µL of 100 mM ABC ...
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bioRxiv - Genetics 2024Quote: ... The injection needle (Femtotips II, Eppendorf) was filled with a mix of 5µg of the plasmid of interest and 1µg of mCherry as an injection control ...
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bioRxiv - Biophysics 2023Quote: ... The injection needles Femtotip II (Eppendorf) were used for injection ...
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bioRxiv - Cell Biology 2022Quote: ... Upper organic phase with collected and subsequently dried in vacuo (Eppendorf concentrator 5301, 1 ppm).
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bioRxiv - Cell Biology 2022Quote: ... Upper organic phase was collected and subsequently dried in vacuo (Eppendorf concentrator 5301, 1 ppm).
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bioRxiv - Genomics 2024Quote: ... The aqueous phase solution was transferred to a new 1.5 mL centrifuge tube (Eppendorf, 05414203). 500 μl of 100% isopropanol (MilliporeSigma ...
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bioRxiv - Immunology 2024Quote: ... The optical density of–log-phase cultures was determined using a photometer (Eppendorf, Hamburg, Germany). Cultures with an optical density of 0.1 ...
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bioRxiv - Biochemistry 2024Quote: ... Upper organic phase with collected and subsequently dried in vacuo (Eppendorf concentrator 5301, 1 ppm).
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bioRxiv - Cell Biology 2022Quote: ... The fully dried gel slices were incubated with 1 µg trypsin per tube in 50 mM ammonium bicarbonate buffer at 37°C overnight on a ThermoMixer (Eppendorf). Digested peptides were extracted twice with 150 µl of 30% acetonitrile ...
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bioRxiv - Biochemistry 2020Quote: ... chloroform (4:2:1, v: v: v, containing 20 mM ammonium formate. The solutions were then placed into the wells of an Eppendorf twin-tec 96-well PCR plate ...
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bioRxiv - Cancer Biology 2023Quote: ... The beads-captured proteins were digested overnight at 37 ℃ with 0.5 μg trypsin/LysC mix in 25 mM ammonium bicarbonate under vigorous shaking (1200 rpm, Eppendorf Thermomixer). The supernatants were collected and the peptides were purified by a modified Sp3 clean-up protocol and finally solubilized in the mobile phase A (0.1% formic acid in water) ...
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bioRxiv - Molecular Biology 2023Quote: ... the rehydrated gel pieces were overlaid with 30 μL of 0.01% ProteaseMAX in 50 mM ammonium bicarbonate and incubated at 37°C for 3 hours with shaking at 800 rpm (Thermomixer, Eppendorf). The digestion reaction was transferred to a fresh tube ...
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bioRxiv - Neuroscience 2023Quote: ... The beads-captured proteins were digested overnight at 37 °C with 0.5 μg trypsin/LysC mix in 25 mM ammonium bicarbonate under vigorous shaking (1200 rpm, Eppendorf Thermomixer). The supernatants were collected ...
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bioRxiv - Biochemistry 2024Quote: ... The beads-captured proteins were digested overnight at 37 ℃ with 0.5 μg trypsin mix in 25 mM ammonium bicarbonate under vigorous shaking (1200 rpm, Eppendorf Thermomixer). The supernatants were collected and the peptides were purified by a modified Sp3 clean-up protocol and finally solubilized in the mobile phase A (0.1% FA in water) ...
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bioRxiv - Biophysics 2020Quote: ... mounted to a micromanipulator (Injectman II, Eppendorf).
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bioRxiv - Plant Biology 2021Quote: ... 80 μL of the upper phase were transferred to a new 2 mL reaction tube (Eppendorf) and evaporated under constant N2 stream ...
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bioRxiv - Cancer Biology 2021Quote: ... The combined upper phases were dried under vacuum in an Eppendorf Concentrator 5301 (Eppendorf, Hamburg, Germany) at 30°C.
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bioRxiv - Cancer Biology 2021Quote: ... 300 μl of the polar (upper) phase were dried in a vacuum concentrator (Eppendorf Concentrator Plus) without heating for derivatization.
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bioRxiv - Biophysics 2023Quote: ... Exponential phase cells (OD600 = 0.5-0.6) were then pelleted at 5000 rpm (Centrifuge 5425, Eppendorf, Germany) for 5 min at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... The phase separated solution was then incubated at respective temperatures in a thermocycler (Mastercycler nexus, Eppendorf) for 20 minutes ...
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bioRxiv - Genomics 2024Quote: ... the upper aqueous phase was collected and transferred to a new 1.5 ml microcentrifuge tube (Eppendorf). RNA samples were precipitated by adding 500 μl of isopropanol followed by overnight incubation at -20°C and centrifugation at 7,500 g for 15 minutes at 4°C ...