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Citations for Eppendorf :
651 - 700 of 1356 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... fractions numbered from 9 to 22 were processed by centrifugation (20,800 g for 30 min at 4 °C, Rotor F45-30-11, Eppendorf), and the resulting pellet was further resuspended in 160 μL of alkaline carbonate buffer (100 mM Na2CO3 ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were washed in 20 mL of chilled phosphate-buffered saline (1X PBS, pH 7.4), and centrifuged (1500 ×g, 4 °C, 15 min) using the Centrifuge 5810R (Eppendorf). The cell pellet was subsequently resuspended in 30 mL of ice-cold RSB hypo buffer (10 mM NaCl ...
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bioRxiv - Bioengineering 2024Quote: ... nanoparticles were incubated with 55% plasma (with nanoparticles’ concentration of 0.2 mg/ml) for 1h at 37 °C at a constant agitation (total volume: 9’ s1.5 mL Eppendorf tubes). To remove unbound and plasma proteins only loosely attached to the surface of nanoparticles ...
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bioRxiv - Microbiology 2024Quote: ... followed by centrifugation at 21,130 x g at 4°C for 10 min in a tabletop chilled centrifuge (Eppendorf). 250 μl of the clarified lysate was transferred to a pre-chilled tube and mixed with 750 μl of TRIZOL LS (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... The resulting parasite solution was pelleted at 1,000 x g at 4°C for 8 min using a chilled large tabletop centrifuge (Eppendorf). The pellet obtained from two D150 plates underwent three washes with intracellular buffer before being resuspended in 200 μl of ice-cold polysome lysis buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... The parasite solution was pelleted at 1,200 x g at 4°C for 10 min using a chilled large tabletop centrifuge (Eppendorf). The resulting pellet underwent 3 washes with intracellular buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Blood was collected into SAFE-T-FILL capillary collection tubes (RAM Scientific) on ice and then centrifuged at 4°C for 10 min at 14,000 rpm (Centrifuge 5804R, Eppendorf). Plasma was aliquoted into Eppendorf tubes and stored at -80°C ...
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bioRxiv - Microbiology 2024Quote: ... The cells were collected by centrifugation at 4,000 rpm for 10 minutes at 4°C using S-4x universal rotor (Eppendorf). Cell pellets from the 200 mL culture were dissolved in cell suspension buffer (20 mM HEPES:KOH pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... The cells from a 25-mL culture were collected by centrifugation (4,000 rpm, 10 min, at 4°C in S-4x universal rotor (Eppendorf)) and resuspended in 0.5 mL of 3 M NaOAc pH4.5 supplemented with 10 mM EDTA ...
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bioRxiv - Microbiology 2024Quote: ... The cells from 50 mL culture were collected by centrifugation (4,000 rpm, 10 min, at 4°C in S-4x universal rotor (Eppendorf)) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and the samples were incubated overnight (55 °C at 1100 rpm) in a thermomixer (model 5350; Eppendorf, Hauppauge, NY). After incubation ...
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bioRxiv - Physiology 2024Quote: ... The next day we centrifuged tubes with methanol and tissue (4°C, 2000rpm) for fifteen minutes (Eppendorf centrifuge 5810R). We carefully pipetted the supernatant (∼ 5mL ...
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bioRxiv - Microbiology 2020Quote: BV2 cells were maintained in cell culture dishes (Eppendorf) in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Biochemistry 2024Quote: ... the pre-weighed mouse tissues were transferred to Protein LoBind 1.5 mL microcentrifuge tubes (Eppendorf) using 500 µL cold sterile DPBS ...
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bioRxiv - Physiology 2022Quote: The pTr cells were seeded on cell imaging cover glasses with four chambers (Eppendorf; 105 cells/chamber). RNA cargo carried by EVs prepared as described above was stained with thiazole orange as described elsewhere ...
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bioRxiv - Physiology 2023Quote: ... pTr cells were cultured in Cell Imaging Coverglasses (Eppendorf, Germany) and subjected to miRNA transfection as described above ...
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bioRxiv - Cell Biology 2020Quote: ... we centrifuged the crude lysate at 500 g for 5 min at 4°C in a microfuge (Eppendorf, model 5424R) to remove cell debris ...
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bioRxiv - Developmental Biology 2021Quote: ... Japan) followed by a 1-hr incubation at 25 °C with intermittent vortexing using a thermomixer (Eppendorf, Wesseling-Berzdorf, Germany). Aliquots were designated as the RIPA-insoluble fraction and subsequently mixed with LDS sample buffer and boiled at 95 °C for 3 min before being stocked frozen at -30 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM label was subsequently added and samples were incubated over night at 4°C and 350 rpm in a Thermomixer (Eppendorf). Excess label was removed by using a Sephadex G25 column (GE Healthcare Bio-Science AB ...
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bioRxiv - Bioengineering 2022Quote: ... The lysates were clarified by centrifugation at 18,500g at 4 °C for 30 minutes using tabletop centrifuge (Eppendorf Centrifuge 5810R). Soluble protein was separated from cell debris and the supernatant was passed through 5 mL HisTrap columns (Cytiva ...
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bioRxiv - Biochemistry 2022Quote: ... at a final concentration of 1 mM for 30 min at 30 °C while shaking at 650 rpm in a Thermomixer (Eppendorf). The reaction was quenched with ammonium bicarbonate at a final concentration of 50 mM for 10 min at 30 °C and 650 rpm ...
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bioRxiv - Neuroscience 2021Quote: ... Single EGFP+ cells were sorted on a BD Influx equipped with a 140 µm nozzle and a cooling unit with a sample temperature of 4°C and collected into a DNA LoBind tube (Eppendorf) containing aCSF with 0.5% BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... The samples were incubated on ice for 10 minutes and then at 37°C for 10 minutes at 700 rpm shaking on a Thermomixer (Eppendorf). Lysates were cleared by centrifugation at 15,000 g for 2 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA was eluted in 30 μL nuclease-free water and stored at −80°C in low-bind tubes (Eppendorf) until further analysis.
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bioRxiv - Genomics 2021Quote: ... Nuclei were rotated at 4 °C for 5 minutes before being pelleted again with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). After centrifugation ...
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bioRxiv - Genetics 2020Quote: ... The supernatant solutions were centrifuged at 15,800xg for 15 min at 4°C in a microcentrifuge (Model 5042, Eppendorf, Inc.) and the resultant supernatant fractions were stored at −80°C ...
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bioRxiv - Genomics 2021Quote: ... nuclei were rotated at 4 °C for 5 minutes before being pelleted again with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). After centrifugation ...
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bioRxiv - Cell Biology 2021Quote: ... Relative amount of bacteria on seeded plates was measured by washing bacteria off plates with M9 buffer following a 24 hour temperature shift to 25°C and OD600 was measured using a BioPhotomer D30 (Eppendorf).
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bioRxiv - Biophysics 2021Quote: ... at a final ratio of 2.5 nmol DSS per 1 µg protein for 30 min at 37 °C while shaking at 650 rpm in a Thermomixer (Eppendorf). After quenching by addition of ammonium bicarbonate to a final concentration of 50 mM and incubation for 10 min at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The reactions were carried out in a final volume of 50 μL by incubation by shaking in a Thermomixer C (Eppendorf) at 750 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions were started by addition of 59.0 μM ubiquitin and 2 mM ATP then incubated at 37°C in a Mastercycler nexus (Eppendorf, Germany) thermocycler for 2 hrs unless otherwise stated ...
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bioRxiv - Biochemistry 2020Quote: ... The resin was pelleted down by centrifugation at 4°C (1258 g for 5 min, Eppendorf #A-4-81 rotor) and washed with ice-cold 50 mL Binding buffer composed of 10 mM imidazole (pH 7.4) ...
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bioRxiv - Neuroscience 2020Quote: ... the pellet was resuspended in SK buffer (LS buffer, 1% sarkosyl, 500mM NaCl) and solubilized at 22°C for 60 minutes using a ThermoMixer 5350 (Eppendorf) at 500rpm ...
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bioRxiv - Microbiology 2021Quote: ... The samples were immediately cooled on ice and centrifuged for 12 min at 4°C and 3700 rpm (Centrifuge 5920 R, S-4×1000, Eppendorf). The cell pellets were stored at −20°C until RNA extraction.
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bioRxiv - Microbiology 2021Quote: ... The cells were transferred into anaerobic 50 mL reaction tubes inside the anaerobic chamber and harvested outside of the anaerobic chamber for 12 min at 4°C and 3700 rpm (Centrifuge 5920 R, S-4×1000, Eppendorf). The supernatant was discarded inside the anaerobic chamber and the pellet was resuspended in fresh RCM medium to an OD600 of 5-10 ...
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bioRxiv - Microbiology 2021Quote: ... the cells were transferred into the anaerobic chamber and harvested for 12 min at 25°C and 3700 rpm (Centrifuge 5920 R, S-4×1000, Eppendorf) outside of the anaerobic chamber ...
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bioRxiv - Cell Biology 2021Quote: ... after which the protein samples were denatured at 650 rpm for 1 h at 37°C using a Thermomixer (Eppendorf). Sample reduction and alkylation were individually performed by adding 10 mM dithiothreitol and 40 mM iodoacetoamide ...
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bioRxiv - Neuroscience 2019Quote: ... The lysate was cleared from the insoluble fraction by centrifugation for 15 min at 10,000 rpm (11,292 x g) at 4 °C (Eppendorf 5427 R). The supernatant was retrieved and the procedure repeated until no pellet was visible after centrifugation ...
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bioRxiv - Biochemistry 2021Quote: ... 33 mg of MFC tissue stored at −80°C was transferred to 1.5 mL LoBind Eppendorf tubes (Eppendorf, Cat# 022431081) and combined with 0.5 mL homogenization buffer consisting of 8 M urea ...
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bioRxiv - Biochemistry 2021Quote: ... at a molar ratio crosslinker to lysines of ∼2.7 for 30 min (except for the time-course experiments, see below) at 37°C shaking at 650 rpm in a Thermomixer (Eppendorf). Protein samples were quenched by addition of ammonium bicarbonate to a final concentration of 50 mM and either directly evaporated to dryness or after an additional centrifugation step for 60 min at 21 000 x g in order to separate the condensed from the dilute phase ...
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bioRxiv - Biochemistry 2020Quote: ... Cultures were grown with shaking at 200 rpm at 30 °C on an Innova 2300 platform shaker (Eppendorf, Hamburg, Germany) to maximal culture density ...
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bioRxiv - Microbiology 2020Quote: Cells were concentrated from 10 ml of the enrichment cultures by centrifugation (4500×g, 20 min, 28°C) using a swing-bucket rotor (Eppendorf) and resuspended in approximately 200 µl of the supernatant ...
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bioRxiv - Plant Biology 2020Quote: ... Each insoluble fraction was digested by 0.5 μg chymotrypsin and 1 μg trypsin at 37°C for 20 h shaking at 600 rpm (Eppendorf tabletop). Digestion reactions were stopped using TFA to a final concentration of 0.5% ...
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bioRxiv - Plant Biology 2021Quote: ... samples were derivatized with N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (Sigma-Aldrich, 1.0 mg per sample, pH 4.0, 60 min, 37 °C under continuous shaking in an Eppendorf thermomixer) and analysed using a UPLC-MS/MS equipped with an electrospray interface in positive mode (ACQUITY ...
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bioRxiv - Microbiology 2021Quote: ... The dialysis block was then incubated at 37°C for 6 hours with constant shaking at 400rpm (Thermomixer comfort, Eppendorf). After 6 hours ...
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bioRxiv - Microbiology 2021Quote: ... cells were cultured in a test tube at 30°C and optical density at 600 nm (OD600) was recorded using a spectrophotometer (Eppendorf). To obtain doubling time ...
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bioRxiv - Systems Biology 2021Quote: ... Complementary sequences were annealed at equimolar concentrations by heating mixtures to 95 °C for 10 min in a thermal block (Eppendorf) followed by their cooling to room temperature (RT ...
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bioRxiv - Microbiology 2019Quote: ... PMNs were sedimented for 10 min with 1000 × g at 4°C on 45° fixed-angle rotor FA-45-30-11 (Eppendorf). The supernatant was filtered by gravity through sterile PVDF 5.0 µm Millex syringe filters (Merck-Millipore) ...
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bioRxiv - Cancer Biology 2020Quote: ... at a concentration of 100μg/ml for 1 hr at 37°C in a heat block (Thermo mixer, Eppendorf, Germany). After incubation 10μg/ml of propidium iodide was added in dark for 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... were vortexed 3 min at room temperature followed by centrifugation at 4,500 x g for 5 min at 4°C (Eppendorf #5010R). The supernatant fluid was poured into a chilled 2 ml screw-capped tube ...