Labshake search
Citations for Eppendorf :
701 - 750 of 1007 citations for 2 Methyl 4 piperidin 1 ylsulfonyl phenylboronic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: ... The nuclei were diluted to ∼300 nuclei per uL across 2 x twin.tec™ 96 Well LoBind PCR Plates (Eppendorf, 0030129512). The reverse transcription reaction was set up as in Cao et al. ...
-
bioRxiv - Microbiology 2023Quote: ... 200 μl of the sample was mixed with an equal volume of buffered phenol and 20 μl of 20% SDS in a 2 mL centrifuge tube (Eppendorf, Germany). After adding 0.5g of 2 mm zirconia beads (BioSpec Inc. ...
-
bioRxiv - Plant Biology 2023Quote: ... The extracted samples were aliquoted in 2 mL tubes and vaporized under vacuum at 40°C using SpeedVac (Eppendorf, Hamburg, Germany). The samples were resuspended and further diluted in sterile water before applying to MBA ...
-
bioRxiv - Biophysics 2023Quote: ... were coated with Expi293 cell produced RS2 at 4 µg/mL concentration in 1x PBS (60 µl/well) and incubated for 2 h at 25 °C under gentle shaking condition (300 rpm) on a thermomixer (Eppendorf, USA) and then plate was transferred to 4 °C cold room for overnight ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were then exposed to phages at indicated MOIs or incubated untreated for 30 min in a 2 mL Eppendorf tube under shaking conditions (<650 rpm in an Eppendorf ThermoMixer). For TIRF microscopy ...
-
bioRxiv - Bioengineering 2023Quote: ... Digested material was then eluted from the column by centrifuge at 1000 rpm for 2 minutes and dried in speedvac (Eppendorf, Germany). Each prepared sample was separated on Hi-pH column (Thermo ...
-
bioRxiv - Biochemistry 2024Quote: Sieved soil and the extraction buffer consisting of 5% SDS in 25 mM Tris-HCL (pH 8.0) were combined in a 2 mL Eppendorf Lo-Bind tube (Eppendorf, Hamburg, Germany) and vortexed for 10 seconds to mix ...
-
bioRxiv - Bioengineering 2024Quote: ... The 12 initial cultivations were executed in a DASGIP© Parallel Bioreactor System (max. working volume 2 L; Eppendorf, Hamburg, Germany) as described in [51] ...
-
bioRxiv - Microbiology 2024Quote: ... The NPA positive was diluted in 2 ml of MEM without FBS and centrifuged at 200Xg for 5 min (HSR Centrifuge Eppendorf Presvac) (NPA supernatant) ...
-
bioRxiv - Biochemistry 2020Quote: Microdissected tissue samples were lysed in SDT buffer (4% SDS, 0.1M DTT, 0.1M Tris/HCl, pH 7.6) in a thermomixer (Eppendorf ThermoMixer® C, 30 min, 95°C, 750 rpm). After that ...
-
bioRxiv - Microbiology 2022Quote: ... Trophozoites were harvested from their growth support by incubating the tubes by tapping the glass tubes followed by centrifugation (Eppendorf centrifuge 5810R, rotor A-4-62) according to a previously reported protocol 84.
-
bioRxiv - Bioengineering 2024Quote: ... Cultures were further incubated overnight (900 RPM, 20 °C, 70 % humidity, 20 h) and harvested by centrifugation (Eppendorf 5810R; 3,220 x g, 4 °C, 15 min). The obtained cell pellet was resuspended with 150 µL buffer (50 mM Bicine ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... The sequencing failed for 1 sample and 1 sample was damaged during the travel (Eppendorf tube burst), decreasing our total sample size to 41 ...
-
bioRxiv - Plant Biology 2024Quote: ... Duplicate samples of 1 mL were incubated for 1 h at 50 °C in a thermomixer (Eppendorf) with shaking at 600 rpm with 5 µL of CTec3 diluted 10-fold from the commercial product added to each sample ...
-
bioRxiv - Cell Biology 2020Quote: ... The chondrocytes were incubated with the dye at 37°C for 2 hours at 300 rpm in a ThermoMixer C (Eppendorf, Hamburg, Germany). After incubation ...
-
bioRxiv - Cell Biology 2020Quote: ... the medium exchange was conducted under semi-sterile conditions directly at the microscope with a 10 μl microloader tip (Microloader Tip 0,5-10 μl / 2-20 μl, Eppendorf AG, Hamburg, Germany) every two days.
-
bioRxiv - Biochemistry 2021Quote: ... Cells were maintained in a humidified atmosphere of 5% CO2 and 37 °C and were passaged every 2-3 days into 10 cm polystyrene coated plates (Eppendorf; EP0030700112-300EA) upon reaching high density ...
-
bioRxiv - Genetics 2022Quote: ... we inoculated yeast strains into 400 µL of liquid SC -lys medium with G418 for overnight growth in 2 mL 96 well plates at 30 °C with 1000 rpm mixing on a MixMate (Eppendorf, Hamburg, Germany). The next day ...
-
bioRxiv - Biophysics 2022Quote: ... Ltd., Kanagawa, Japan) controlled by a pneumatic microinjector (IM-11-2, Narishige, Tokyo, Japan) and a micromanipulator (Transferman NK2, Eppendorf, Hamburg, Germany) and transferred into 4 ml RNase-free water (06442-95 ...
-
bioRxiv - Microbiology 2021Quote: ... the plate was returned to the magnet and for 2 minutes and 48 μl of purified DNA was transferred to a fresh 96-well PCR plate (Eppendorf 0030 128.648).
-
bioRxiv - Biochemistry 2022Quote: ... and samples were vortexed at 1200 rpm for 2-3 hours at room temperature (MixMate ®, Eppendorf South Pacific, Sydney, NSW Australia). Following this ...
-
bioRxiv - Neuroscience 2021Quote: ... a whole eye from wild type and mutant adult zebrafish or 100 zebrafish mutant larvae were transferred to 2 ml Eppendorf microcentrifuge tubes (Eppendorf, Hamburg, Germany). 200 μl of 2 M hydroxylamine (pH 8 ...
-
bioRxiv - Immunology 2022Quote: ... Purified IgG was digested into F(ab’)2 with 200 μg of IdeS per 10 mg of IgG for 6 hr on a thermal mixer (Eppendorf ThermoMixer C) at 37°C ...
-
bioRxiv - Bioengineering 2024Quote: ... Samples were diluted to 2 x 107 cells per mL and 50 µL of each sample was added to 5 mL tubes (Eppendorf, Hamburg, DE), to constitute 1 million cells per flow sample.
-
bioRxiv - Genomics 2024Quote: ... and Cas9 protein (100 ng/μl) were injected into individual eggs using a FemtoJet and InjectMan NI 2 microinjection system (Eppendorf, Hamburg, Germany). The microinjection process was completed within 2 hours ...
-
bioRxiv - Biochemistry 2024Quote: ... four times (1600 μL) ice-cold 80% acetone was added to 400 μL of urine in 2 ml protein Lo-Bind tubes (Eppendorf, Hamburg, Germany). After incubating at −20 °C for 1hr ...
-
bioRxiv - Genetics 2024Quote: ... and Cas9 protein (200 ng/μl) were injected into individual eggs using a FemtoJet and InjectMan NI 2 microinjection system (Eppendorf, Hamburg, Germany). The whole process was completed in two hours ...
-
bioRxiv - Biochemistry 2021Quote: ... Unreacted dye was removed by two passages over an equilibrated Bio-spin® 6 column filled with Bio-gel P-6 media in labeling buffer and centrifuged in a tabletop centrifuge (Eppendorf 5810 R, rotor A-4-62) at 1500 rpm for 3 min ...
-
bioRxiv - Cancer Biology 2024Quote: ... 3 times for 5 seconds each to ensure homogenization and centrifuged at 14000 x g for 10 minutes at 4° C (Eppendorf centrifuge 5424 R, FA-45-24-11 rotor). Supernatant was precleared by rotating 20 µl Protein A agarose beads (Cell Signaling #9863 ...
-
bioRxiv - Bioengineering 2020Quote: ... Single colonies were grown overnight in 1 mL of SC or SC-ura medium supplemented with 2% glucose in individual wells of the 24-well microtiter plates under constant blue light in an orbital shaker (Eppendorf, New Brunswick, USA) at 30°C and at 200 rpm agitation ...
-
bioRxiv - Systems Biology 2021Quote: ... Cells were resuspended and 50 μl of each pre-culture was used to inoculate 1.5 mL of fresh YPD media (corresponding to an optical density of 0.5 at 600 nm) in 96-deep-well plates (LoBind®, 2 mL, cat no. 0030504305, Eppendorf AG, Hamburg, Germany). Plates were covered with an air permeable membrane and incubated while shaking at 300 rpm and 30 °C for 6 hours ...
-
bioRxiv - Biophysics 2020Quote: ... Supplementary Table 5) were incubated together for 2 h at 37 °C under gentle shaking (450 rpm, Eppendorf ThermoMixer® C, Eppendorf AG, Germany). Unbound DNA origami was removed by placing the tube on a magnet and discarding the supernatant ...
-
bioRxiv - Cell Biology 2021Quote: ... of the same volume as the RIPA buffer at 25 °C for 2 h with intermittent mixing (Thermomixer comfort, Eppendorf, Wesseling-Berzdorf, Germany). The digested sample was labelled as the RIPA-insoluble fraction (Ins) ...
-
bioRxiv - Systems Biology 2024Quote: ... placed in a 2mL Protein LoBind® Eppendorf tube and centrifuged at 1000g for 2 minutes at RT (Microcentrifuge 5415, Eppendorf, Hamburg, Germany).
-
bioRxiv - Microbiology 2023Quote: ... algal cells with the attached bacteria in the treatment with the species consortium were also collected by a pipette from the formaldehyde-fixed suspended sample and transferred into 2 ml safe-lock tubes (Eppendorf, Wesseling-Berzdorf, Germany). To avoid an excessive fixation ...
-
Identification of ABHD6 as a regulator of lysophosphatidylserines in the mammalian liver and kidneysbioRxiv - Biochemistry 2024Quote: ... 50 μL of 1mg/mL soluble or membrane proteomic fraction from mammalian cells or tissues were incubated with 2 μM FP-rhodamine for 45 minutes at 37°C with constant shaking (Eppendorf, Thermo Mixer C). The reactions were quenched by adding 20 μL of 4x SDS-PAGE loading buffer followed by boiling for 5 minutes ...
-
bioRxiv - Microbiology 2024Quote: ... by five 20 second pulses at speed 4350 rpm with chilling on ice for 2 min between the cycles and clarified by centrifugation at 21,300 rcf for 20 min (Eppendorf 5425R, rotor FA-24x2). Clarified lysates were normalized to 1500 ng/µl and loaded onto 10 – 40% sucrose gradients in gradient buffer and run for 3 hours at 30,000 rpm at 4°C in an SW-41Ti rotor ...
-
bioRxiv - Biochemistry 2023Quote: ... and the protein concentration determined by nanodrop (assuming an extinction coefficient of εMolar = 46410 M−1cm−1. The protein solutions were moved into 1 mL aliquots in 1.5 mL Eppendorf tubes ...
-
bioRxiv - Microbiology 2022Quote: ... trypsin was inactivated with 1:1 complete media and the cell suspension was collected into a 5mL protein-low bind centrifuge tube (Eppendorf). To remove residual trypsin ...
-
bioRxiv - Cancer Biology 2022Quote: ... Pups from these females were injected at P2 with the two plasmids mixed at a ratio of 1:1 using a Femtojet microinjector (Eppendorf) directly into the lateral ventricle ...
-
bioRxiv - Neuroscience 2020Quote: ... and RNA concentration was measured in a 1 μl sample diluted 1:200 in nuclease-free water in a spectrophotometer (BioPhotometer, Eppendorf). cDNA was synthesized using 1 μg of input RNA with an iScript™ cDNA Synthesis Kit and MyIQ thermocycler (both Bio-Rad ...
-
bioRxiv - Neuroscience 2023Quote: ... The 1 ml of PBS with the resuspended cells was transferred to a 1·5 ml Protein LoBind tube (Eppendorf), centrifuged at 3000 x g for 6 min at 4 °C ...
-
bioRxiv - Developmental Biology 2021Quote: ... or double (for qRT-PCR) blastomere microinjections of 2-cell (E1.5) stage embryos were performed using the FemtoJet 4i (Eppendorf, Hamburg, Germany; Cat. No. 5252000013) micro-injector ...
-
bioRxiv - Cell Biology 2020Quote: ... This solution was centrifuged at 3000g for 2 min and the supernatant was collected and concentrated in a vacuum concentrator in vacuum-alcoholic (V-AL) mode (Eppendorf plus, Eppendorf, Hamburg, Germany). The concentrated crystals were resuspended in 100μl of 1 X PBS and Nuclease Free Water (NFW) ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were loaded into a microinjection needle and 50 nL of the ZMEL cell suspension was transplanted into the hindbrain ventricle of anesthetized 2 dpf zebrafish larvae by using an oil-controlled microinjection rig (Eppendorf CellTram 4r Oil, #5196000030) with a Narishige arm ...
-
bioRxiv - Genomics 2024Quote: ... 2 µL sheared DNA was used to perform overnight pulse-field gel electrophoresis while the remaining sheared DNA was stored in a 2 mL DNA LoBind® Tube (Eppendorf Cat. No. 022431048) at 4 °C overnight ...
-
bioRxiv - Bioengineering 2023Quote: ... Transwells® were then incubated at 37 °C and 25 RPM for 2 hrs in a New Brunswich™ Innova® 40 shaker (Eppendorf, Enfield, CT). Samples were collected every hour from the basolateral side ...
-
bioRxiv - Microbiology 2023Quote: ... Each flask was aeriated with 2% v/v CO2 enriched air which was controlled by a DASGIP® MX module (Eppendorf AG, Hamburg, Germany). The microalgae starting with an OD750 of 0.1 were cultivated in modified Johnson medium at a pH of 7.5 [49] with 1 M NaCl ...
-
bioRxiv - Neuroscience 2024Quote: ... The forebrain was dissected and weighed before lyophilization at a shelf temperature of –56 °C for 24h under vacuum of 0.2 mBar (Christ LMC-1 BETA 1-16) and extraction with formamide at 57°C for 24h on a shaker at 300 rpm (Eppendorf Thermomixer). Integrated density of tracer fluorescence was determined in triplicates after 1:3 ethanol dilutions ...
-
bioRxiv - Microbiology 2023Quote: ... 1 min), annealing (65°C, 1 min), and elongation (72°C, 1 min) were performed in a Mastercycler thermal cycler (Eppendorf, Germany). A final extension step was added for 10 min at 72 °C at the end of the 30th amplification cycle ...