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Citations for Eppendorf :
651 - 700 of 1007 citations for 2 Methyl 4 piperidin 1 ylsulfonyl phenylboronic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Isolated midguts or the mosquito carcass (remainder of the body without midgut) were placed separately into 2 ml safe-lock Eppendorf tubes (Eppendorf) containing 250 µl of mosquito diluent (1 × PBS supplemented with 20% FBS ...
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bioRxiv - Biochemistry 2022Quote: ... Annealing was carried out by heating the mixture to 95 °C for 2 minutes followed by gradual cooling to 25 °C over 45 minutes in a thermocycler (Eppendorf).
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bioRxiv - Developmental Biology 2020Quote: ... or double (for qRT-PCR) blastomere microinjections of 2-cell (E1.5) stage embryos were performed using FemtoJet or FemtoJet 4i (Eppendorf; 5252000013) micro-injectors ...
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bioRxiv - Neuroscience 2020Quote: ... or vehicle for thirteen days from postnatal day 2 (P2) to postnatal day 14 (P14) using a micropipette (0.5-10 µL; Cat. No. 3123000020; Eppendorf, Germany). Mouse pups for each postnatal treatment experiment were selected from at least six different dams to minimize any litter-related bias ...
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bioRxiv - Biochemistry 2021Quote: ... and a concentration of 5 µg / ml of the plasmid with GFP (Green Fluorescent Protein) were transferred to an electroporation cuvette (2 mm wide and 400 µl in volume (Eppendorf)) ...
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bioRxiv - Neuroscience 2022Quote: ... cell pellets were resuspended in 1mL of ice-cold PBS and transferred to 2 mL LoBind Eppendorf tubes (Eppendorf #022431102). Cells were centrifuged for 2 minutes at 800rpm before being flash-frozen on dry ice ...
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bioRxiv - Cell Biology 2020Quote: ... Approximately 0.5µl AAV8 (∼2×1010GC) were introduced into the subretinal space using a pulled angled glass pipette controlled by a FemtoJet (Eppendorf). Adult mice were anesthetized with an intraperitoneal injection of a mixture of ketamine and xylazine ...
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bioRxiv - Molecular Biology 2020Quote: ... The obtained pellets were air-dried to rid them of residual acetone and solubilized with 120 μl Tris-SDS (10 mM Tris-HCl pH 7.5 and 2% SDS) at 56° C for 10 min and shaking at 1000 rpm in a table-top thermomixer (Eppendorf). The resulting suspension was centrifuged 20,000×g for 10 minutes at room temperature in a table-top micro-centrifuge (Eppendorf) ...
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bioRxiv - Molecular Biology 2020Quote: The cell cultures were then transferred into 2 ml reaction tubes and placed in slots in a table-top thermomixer (Eppendorf) set to 37° C and shaking at 700 rpm for five minutes prior to pulse-labelling ...
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bioRxiv - Immunology 2021Quote: ... 293T-hACE2 cells were transfected with 0.5 μg of SARS-CoV-2-RBD plasmid (a gift from Dr. Abraham Pinter) in each well in a 24-well plate (Eppendorf) for 48 hours at 37°C under 5% (v/v ...
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bioRxiv - Genomics 2020Quote: ... was used to pool the whole 384-well plate containing the barcoded cDNA into a single 2 ml DNA LoBind tubes (Eppendorf) and cleaned up using Sera-Mag Select beads (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... 293T-hACE2 cells were transfected with 0.5 μg of SARS-CoV-2-RBD plasmid (a gift from Dr. Abraham Pinter) each well in a 24-well plate (Eppendorf) for 48 hours at 37°C under 5% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were pelleted (600 x g, 2 min) and the supernatant was transferred to a fresh low protein binding tube (Eppendorf). The beads were washed with 2 × 300 μL HPLC grade water and the washes combined with the original supernatant ...
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bioRxiv - Physiology 2020Quote: ... with 3 mM Tris(2-carboxyethyl)phosphine hydrochloride(TCEP-HCl) (Thermo Pierce) while shaking at 600 rpm in a thermomixer (Eppendorf). Samples were alkylated with 9 mM iodoacetamide (22 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Unincorporated biotin-HPDP was removed by two rounds of chloroform extraction with 2 ml Phase lock gel heavy tubes (Eppendorf). RNA was precipitated with 1/10 volume of 5M NaCl and an equal volume of Isopropanol ...
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bioRxiv - Biochemistry 2023Quote: ... then added to 200 μL of Pierce magnetic streptavidin resin (cat. # 8817) in a 2 mL low-binding tube (Eppendorf). Proteins were captured by continuous rotation at room temperature for 1.5 hours ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 3.5Lμl 2-β mercaptoethanol (#444203, MilliporeSigma) was added directly to the beads and incubated with mixing on a ThermoMixer (Eppendorf) for 10□min at room temperature ...
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bioRxiv - Genomics 2023Quote: ... supplemented with 3.5 μL 2-β mercaptoethanol was added directly to the beads and incubated with mixing on a ThermoMixer (Eppendorf) for 10 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... the scaffolds were immersed in 1.5 mL BMP-2 (100 µg/mL) diluted in PBS in a low protein-binding falcon tube (Eppendorf, UK). The bottom of the tube was gently tapped on a surface to remove any bubbles between the pores of the scaffold to allow the solution to completely contact the scaffold ...
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bioRxiv - Neuroscience 2024Quote: ... and snap-frozen by placing the tissue in pre-chilled low bind 2 mL microfuge tubes on dry ice (#022431048 Eppendorf). FC sections (male n = 27 ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated at 37°C at 600 rpm with interval mixing (30 seconds mix, 2 minutes pause) on a Thermomixer C (2231000667, Eppendorf). After the last digestion ...
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bioRxiv - Plant Biology 2023Quote: ... the leaves were harvested by cutting along the marked area with a scissor and lyophilized overnight in pre-weighted 2 mL safe-lock tubes (Eppendorf). GCMS and LCMS samples were prepared following the aforementioned methods ...
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bioRxiv - Genomics 2023Quote: ... supplemented with 3.5Lμl 2-β mercaptoethanol (#444203, MilliporeSigma) was added directly to the beads and incubated with mixing on a ThermoMixer (Eppendorf) for 10Lmin at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The flow rate was 0.2 ml/min and 48 × 100 μl fractions were collected in a low-protein binding 96-deep-well plate (Eppendorf) at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: The 2 types of ascospores were cleaned by 2 washes with 10% and 20% sucrose solutions and 10-min centrifugation at 1,000 rpm (desktop centrifuge, Eppendorf, Germany); the supernatant was discarded after each centrifugation ...
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bioRxiv - Biochemistry 2022Quote: ... yeast cells were grown in the rich medium YEP + 2% glucose (YEPD) at 25°C with a rotation of 2000 rpm (New Brunswic Innova 43R, Eppendorf). To arrest cells in late G1 by overexpressing non-degradable Sic1(9m ...
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bioRxiv - Molecular Biology 2023Quote: The fully and semi-ejected ascospores were cleaned separately by 2 washes with 10% and 20% sucrose solutions and 10-min centrifugation at 1,000 rpm (desktop centrifuge, Eppendorf, Germany); the supernatant was discarded after each centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was transferred in a new tube and the cytosolic fraction was dried under vacuum for 2 h at 55°C in a Speedvac (Eppendorf). Pellets were then stored at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and suitable brain areas were quickly dissected from each frozen slice and placed on dry ice in 2 ml tubes (Eppendorf). Each brain was split into 70 slices on average ...
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bioRxiv - Neuroscience 2024Quote: ... Mass Spectrometry Grade (Promega Cat# VA9000 at 100ug/mL in 50mM TEAB. Samples were incubated for 2 hrs at 50°C and mixed at 350 rpm on an Eppendorf ThermoMixer ...
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Revealing the Ultrastructure of Live Candida albicans using Stimulated Emission Depletion MicroscopybioRxiv - Microbiology 2024Quote: ... 200 µL of overnight culture in an Eppendorf tube was spun at 2500rpm for 2 minutes in a microfuge (Centrifuge 5418, Eppendorf) to pellet the cells ...
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bioRxiv - Microbiology 2024Quote: ... Samples were hydrolyzed at 100 °C for 2 h under orbital shaking at 600 rpm (ThermoMixer C, Eppendorf, Hamburg, Germany). After cooling ...
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bioRxiv - Cell Biology 2024Quote: ... then diluted in 2 mL of warm DMEM F12 in a 15 mL Eppendorf conical tube (Eppendorf, Catalog No. 0030122151). Our protocol was adapted from previous reports 34 ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with 500μl of D-PBS and centrifuged at 400xg for 10 minutes at room temperature (Eppendorf Centrifuge 5810R, rotor A-4-62). The supernatant was removed and the cell pellet resuspended in 50μl of D-PBS before being stained with CD20-PE (clone A1SB12 ...
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bioRxiv - Neuroscience 2021Quote: ... were incubated with ten-fold molar excess S-XL6 for 4 hours at 37 °C in protein low bind Eppendorf tubes using Eppendorf Thermomixer at 350 rpm (Eppendorf North America, Enfield, CT, USA). After incubation ...
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bioRxiv - Microbiology 2022Quote: ... 1% oxygen (hypoxic; Eppendorf CellXpert incubator), or 0% oxygen (anoxic ...
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bioRxiv - Cancer Biology 2021Quote: Plasmids were injected into the ventricle of isoflurane-immobilized pups at postnatal day 2 using an Eppendorf Femtojet microinjector (Eppendorf, 5247000030), followed by electroporation (5 square pulses ...
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bioRxiv - Neuroscience 2020Quote: ... For bulk PFF production approximately 500μL of 5mg/ml monomeric protein was added to a 2-ml Eppendorf tube and placed on a thermomixer-C (Eppendorf, USA) shaker and incubated at 37°C and 1000RPM for up to 144 hours ...
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bioRxiv - Systems Biology 2021Quote: ... DW determination was performed by centrifuging 2 ml of the fresh sample placed in a pre-weighed Eppendorf tube in a MiniSpin centrifuge (Eppendorf, Spain) at maximum speed (13.200 rpm ...
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bioRxiv - Genetics 2022Quote: ... Yeast strains were manually inoculated into 400 µL of liquid SC -lys medium with G418 and grown overnight in 2 mL 96 well plates at 30.0 °C with 1000 rpm mixing using a MixMate (Eppendorf, Hamburg, Germany). The following morning ...
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bioRxiv - Microbiology 2022Quote: ... and 100 mg of freeze-dried tissue was transferred to 2-ml microcentrifuge tubes (Eppendorf Safe-Lock tubes; Eppendorf, Hamburg, Germany) containing a single 4-mm stainless-steel grinding ball (SPEX SamplePrep ...
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bioRxiv - Systems Biology 2021Quote: ... DW determination was performed by centrifuging 2 ml of the fresh sample placed in a pre-weighed Eppendorf tube in a MiniSpin centrifuge (Eppendorf, Spain) at maximum speed (13.200 rpm ...
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bioRxiv - Genomics 2021Quote: ... The pellet was resuspended in 400 μl 1XHB buffer and transferred to a 2 ml Eppendorf Protein Lo-Bind tube (Eppendorf, UK). 400 μl of 50% Iodixanol solution (Opti-Prep Density Gradient Medium ...
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bioRxiv - Molecular Biology 2020Quote: ... was incubated with 700 μl hexafluoro-2-propanol (HFIP) overnight and divided into 108 μg doses in LoBind reaction tubes (Eppendorf AG). Samples were lyophilized in a rotational vacuum concentrator system connected to a cold trap (both Martin Christ Gefriertrocknungsanlagen GmbH) ...
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bioRxiv - Neuroscience 2021Quote: ... A plasmid DNA solution of 1.4 µg/uL was injected into embryos using an Eppendorf FemtoJet 4x and Eppendorf InjectMan NI 2 microinjector (Eppendorf; Hamburg, Germany) under a Leica M165 C scope (Leica Microscopes ...
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bioRxiv - Immunology 2022Quote: ... bacterial stocks were grown at 30°C for 2 days to an OD600 of 0.4 approximately 6.3×106CFU/mL (Eppendorf, BioPhotometer; Hamburg, Germany). Bacteria were washed with sterile PBS and aspirated through a 27G needle against the side of the tube multiple times to break up bacterial clumps ...
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bioRxiv - Microbiology 2023Quote: ... 200 μl of the sample was mixed with an equal volume of buffered phenol and 20 μl of 20% SDS in a 2 mL centrifuge tube (Eppendorf, Germany). After adding 0.5g of 2 mm zirconia beads (BioSpec Inc. ...
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bioRxiv - Genomics 2023Quote: ... The nuclei were diluted to ∼300 nuclei per uL across 2 x twin.tec™ 96 Well LoBind PCR Plates (Eppendorf, 0030129512). The reverse transcription reaction was set up as in Cao et al. ...
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bioRxiv - Bioengineering 2024Quote: ... BMP-2 solution was added to FN coated polymers and after 2 h incubation solution was aspirated and collected in Protein LoBind Tubes (Eppendorf™). Enzyme-linked immunosorbent assays (ELISA ...
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bioRxiv - Biochemistry 2024Quote: ... #33045-U) and incubated for 2 h at 60 °C at 400 rpm on a thermomixer (Eppendorf ThermoMixer C, Germany, #EP5382000023). Then ...