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Citations for Eppendorf :
701 - 750 of 910 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... by adding 50 μL of a 2 mg/mL stock solution and 25 μL of 50 mM CaCl2 and incubating for 1 h at 37 °C with agitation at 300 rpm in a Thermomixer comfort (Eppendorf, Germany). The cell wall material was pelleted and washed two times with 2 mL of ultrapure water (21000 g ...
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bioRxiv - Biochemistry 2023Quote: ... The cellulase-LPMO synergy was assessed by performing reactions with crystalline Avicel (1 % w/v) in 50 mM sodium phosphate buffer (pH 6.0) using a thermomixer (Eppendorf, Hamburg, Germany) incubated at 30 °C with horizontal agitation (1000 rpm) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were stained for 1 hour at a temperature of 4°C with shaking at 600 rpm in a Thermomixer comfort (Eppendorf, Germany). Stained cells were washed with ice cold FACS buffer twice before resuspension in FACS buffer for analysis ...
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bioRxiv - Biochemistry 2023Quote: ... was adjusted to 50 ng/μL with 50% ACN containing 0.1% TFA and dispensed in 10 μL each into normal tubes (1.5-mL safe-lock tube, CAT# 0030120086, Eppendorf, Hamburg; Germany), normal polypropylene (PP ...
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Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... A 1 ml sample of overnight suspension was centrifuged for five minutes at 8,000 rpm (Eppendorf MiniSpin F-45-12-11), the pellet resuspended in 1 ml PBS ...
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bioRxiv - Cell Biology 2024Quote: ... The protoplasts were collected by centrifugation at 1700 rpm for 1 minute using a swing-bucket rotor (Eppendorf S-4-72), washed with 15 mL of ice-cold solution 3 (26.4 g of ammonium sulfate ...
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bioRxiv - Physiology 2024Quote: Protein samples were dissolved in 8 M urea in 50 mM ammonium bicarbonate (pH 7.8) by vortexing for 1 hour (Eppendorf ThermoMixer F1.5). 5 mM dithiothreitol (DTT ...
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bioRxiv - Microbiology 2024Quote: ... An overnight culture of 1 ml was pelleted down by centrifugation ((10,000 rpm at 4°C for 10 min; Eppendorf centrifuge 5810R) and washed thrice with M9 minimal (M9M ...
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bioRxiv - Microbiology 2024Quote: ... 1 ml of 108 PFU was centrifuged at 20,000 g (centrifuge 5430 R, rotor FA- 45-24-11HS; Eppendorf, Hamburg, Germany) for 2h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... 100 µl of formic acid 1% were placed in each Millipore Microcon 30 MRCFOR030 Ultracel PL-30 before centrifugation at 14,500 rpm (Eppendorf 5424 centrifuge) for 15 minutes ...
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bioRxiv - Microbiology 2024Quote: ... 1 ml of 108 PFU was centrifuged at 20,000 g (centrifuge 5430 R, rotor FA-45-24-11HS; Eppendorf, Hamburg, Germany) for 2h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... in PBS in a 15mL tube and spun at 1000g for 10 min at room temperature with acceleration and brake set to 1 (Eppendorf 5810R). The supernatant was discarded ...
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bioRxiv - Microbiology 2020Quote: ... After an incubation period of 30 min under shaking (300 rpm, Thermomixer Comfort Typ 5, Eppendorf) at room temperature 65 µL chloroform and bidest ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated at 37°C in humidified 5% CO2 incubators (Eppendorf, GmBH or Thermo Scientific, USA).
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bioRxiv - Neuroscience 2020Quote: ... Microinjection of 300 nl was made over 3 min using a Femtojet injector (~5 psi, Eppendorf), and the exposed cortical surface was covered by a sterilized round cover glass (3 or 4 mm in diameter ...
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bioRxiv - Cancer Biology 2021Quote: ... Cultures were grown in a humidified incubator with 5% CO2 and maintained at 37°C (Eppendorf). The medium was changed every four days ...
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bioRxiv - Cell Biology 2021Quote: ... and then the total elution fractions were dried for 5 h using a Speed-vac (Eppendorf). The samples were stored at –20°C before use in LC-MS/MS analyses.
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bioRxiv - Systems Biology 2022Quote: ... The plate was then centrifuged at 1,000 rpm for 5 min using a centrifuge (Eppendorf, 5810R) that was precooled at the desired temperature (e.g ...
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bioRxiv - Microbiology 2020Quote: ... The plates were then spun at 260g for 5 min at room temperature (Eppendorf 5810R centrifuge) and incubated at 33°C ...
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bioRxiv - Biochemistry 2021Quote: ... and 5% CO2 in a New Brunswick S41i CO2 incubator shaker (New Brunswick Eppendorf, Hamburg, Germany) for 4 days and passaged twice to 0.5×106 cells/mL in a 250 mL and 500 mL shake flask ...
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bioRxiv - Immunology 2020Quote: ... and incubated with stirring at 500 rpm for 5 min at 85°C (Eppendorf, ThermoMixer C). Lysates were either sonicated (Sonifier 250 ...
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bioRxiv - Microbiology 2022Quote: ... Bone marrow cells were pelleted by centrifuging at 1500 rpm for 5 min (Eppendorf centrifuge R5810). Red blood cells were lysed by resuspending the cell pellet in 1 mL of red blood cell lysis solution (150mM NH4Cl ...
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bioRxiv - Neuroscience 2023Quote: ... were mixed and heated for 5 min at 65°C in a Thermal Cycler (Eppendorf, Germany). Next ...
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bioRxiv - Evolutionary Biology 2022Quote: ... its volume was determined using an Eppendorf Xplorer 5–1000 μL electronic pipette (Eppendorf, Hamburg, Germany) and kept homogenous by agitation ...
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bioRxiv - Neuroscience 2023Quote: ... were mixed and heated for 5 min at 65°C in a Thermal Cycler (Eppendorf, Germany). After this step 4 μl of 5x first-strand buffer ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were centrifuged at 1000 rpm for 5 minutes in a MiniSpin Plus microcentrifuge (Eppendorf, Germany). Washed cells were resuspended in 150 μL of FACS buffer and treated with anti-mouse MHC class I antibody conjugated to PE (Clone:28-14-8 ...
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bioRxiv - Biochemistry 2023Quote: ... 5 µL of each reaction was dried using a centrifuge concentrator (Vacufuge plus, Eppendorf, Hamburg, Germany) for 30 min ...
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bioRxiv - Biophysics 2023Quote: ... pH 5) incubated at 37°C for 20h at 300 rpm in a ThermoMixer C (Eppendorf).
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Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... The 5 ml overnight culture was centrifuged at 8,000 rpm (Eppendorf MiniSpin F-45-12-11) for three minutes ...
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bioRxiv - Plant Biology 2024Quote: ... The tubes were then sonicated for 5 min and incubated on a thermomixer (Eppendorf, Hamburg, Germany) at 600 rpm for 60 min at 22°C ...
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bioRxiv - Cell Biology 2024Quote: ... then mixed at 950 rpm for 5 min at 10 °C in an Eppendorf Thermomixer (Eppendorf South Pacific Pty Ltd. ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µM AQT0615) and 10 µL were added in each well with a multichannel pipette (Eppendorf). The 0 % control wells were filled with 10 µL of pure reaction buffer ...
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bioRxiv - Immunology 2022Quote: ... 20 μL of mAb solution was added to the cells in the filter wells and the cells were incubated for 1 h at 4 °C and 750 RPM on a thermomixer (Eppendorf, ThermoMixer C). After the incubation ...
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bioRxiv - Biochemistry 2021Quote: ... all samples were adjusted to the same volume and concentration (ca. 1 mg/mL) and transferred to protein low-bind microcentrifuge tubes (Eppendorf, Hamburg, Germany). To precipitate the proteins ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were eluted with 60 μl of 80% acetonitrile/1% ammonia and dried at 60°C using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Dried peptides were dissolved and sonicated in 5% acetonitrile/0.1% TFA ...
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bioRxiv - Systems Biology 2022Quote: ... Peptides were eluted with 60 μl of elution buffer (80% acetonitrile/1% ammonia) and dried at 60°C using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Dried peptides were redissolved and sonicated in 5% acetonitrile/0.1% TFA ...
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bioRxiv - Biophysics 2022Quote: ... The cultures were then induced with IPTG by adding 10 uL of 100 mM (final concentration approximately 1 mM) per well with an electric repeater pipette (Eppendorf, E4x series), and grown for another 4 h at 37 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/ml of hygromycin and correct genome editing was confirmed by Sanger sequencing (Macrogen Europe ...
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bioRxiv - Developmental Biology 2020Quote: ... Microinjection of young adult hermaphrodite germlines was done using an inverted microinjection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). In cases where the sgRNA target site was not disrupted by sequence integration ...
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bioRxiv - Microbiology 2022Quote: ... 4.6×106 LMBs or NMBs were added and samples incubated at 37 °C for 1 hour on a thermomixer (60 rpm; Eppendorf, Hamburg, Germany). After this step ...
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bioRxiv - Developmental Biology 2021Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/μl of hygromycin (Dickinson et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/μl of hygromycin (Dickinson et al. ...
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bioRxiv - Systems Biology 2024Quote: ... are then added to the sample and incubated at 56°C for 1 hour at 1000 RPM (ThermoMixer C, Eppendorf, Hamburg, Germany). 37µl of 500mM chloroacetamide (50mM final concentration ...
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bioRxiv - Plant Biology 2024Quote: ... SDS at a 1:3 (w/v) ratio and extracted by shaking 1000 RPM 95 °C for using a tabletop shaker (Eppendorf ThermoMixer F2.0). Samples were then centrifuged at 20,000 xg for 10 min at room temperature and the supernatant retained in new tubes ...
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bioRxiv - Microbiology 2023Quote: Liquid samples in 1 ml were collected and centrifuged for 20 min at 21 130 × g at 4°C (Centrifuge 5424 R; Eppendorf, Hamburg, Germany). The supernatants were used for chemical analyses by applying external standards for calibration and quality control ...
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bioRxiv - Synthetic Biology 2023Quote: ... 100 ng corresponding pEcgRNA-N20 and 400 ng donor DNA were co-electrotransformed into 100 uL competent cells in 1 mm gap electroporation cuvette by applying 2.5 kV pulse in electroporator (Eppendorf™ Eporator™). After adding 900 uL of cold SOC medium ...
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bioRxiv - Biochemistry 2024Quote: ... A second gDNA purification was coupled with reverse transcription of RNA (1 μg) into cDNA with the SuperScript IV VILO ezDNase system (Thermo Fischer Scientific) using an Eppendorf Mastercycler (Eppendorf, Hamburg, Germany). cDNA samples were entered into a custom TaqMan low density array (Thermo Fischer Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... castellanii trophozoites were seeded at the log growth phase (1 × 104 cells per well) in a 96-well white microplate (Eppendorf, Hamburg, Germany). Incubation with medium containing varied nitroxoline concentrations ranging from 0 to 100 μM was performed ...
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bioRxiv - Neuroscience 2024Quote: ... Lyophilized peptide was dissolved in 100% HFIP solvent to a final concentration of 1 mM and centrifuged in a speed-vac (Eppendorf, Hamburg, Germany). Peptide pellet was then resuspended in DMSO to a final concentration of 5 mM followed by sonication at 37°C for 10 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... Production of CjLPMO10AFL was achieved by fed-batch fermentation of the expression strain in a 1-liter fermenter (DASGIP® benchtop bioreactors for cell culture; Eppendorf, Hamburg, Germany), essentially as described previously (44) ...