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Citations for Eppendorf :
501 - 550 of 621 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Samples destined for ddPCR assays consisted of randomly selected flies transferred individually to 1.5 mL centrifuge tubes (Eppendorf, 05414203) containing three 2.8 mm ceramic homogenizing beads (VWR ...
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bioRxiv - Physiology 2024Quote: ... plasma samples were thawed from storage at -80°C and centrifuged (5,000 x g, 10 minutes, 4 °C; Eppendorf 5427R with rotor FA-45-48-11 ...
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bioRxiv - Biochemistry 2024Quote: ... Ni-NTA resin equilibrated in buffer A was added over the cleaved laccase samples and incubated at 25 °C and 1,000 rpm in a thermomixer comfort shaker (Eppendorf) for 4 hours ...
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bioRxiv - Biochemistry 2024Quote: ... The protein samples were subsequently aliquoted and dried at 30°C under vacuum centrifugation (Eppendorf 5301 Vacufuge Concentrator System). After rehydration with distilled H2O ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was eluted with 2 x 100 μl of fresh elution buffer (100 mM dithiothreitol in RNase-free H20) directly into 2 ml lobind tubes (Eppendorf) containing 700μl Buffer RLT (RNeasy MinElute Cleanup Kit ...
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bioRxiv - Cell Biology 2021Quote: ... Frozen cell pellets were resuspended in hypotonic buffer and homogenized using a disposable plastic pestle (As One Corp., Osaka, Japan) with matched Safe-Lock tubes (Eppendorf). The detailed procedure is summarized in Fig ...
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bioRxiv - Cell Biology 2021Quote: ... Relative amount of bacteria on seeded plates was measured by washing bacteria off plates with M9 buffer following a 24 hour temperature shift to 25°C and OD600 was measured using a BioPhotomer D30 (Eppendorf).
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bioRxiv - Plant Biology 2020Quote: ... The insoluble fraction was re-suspended in 300 μL 60:40 buffer containing 60% MeOH: 40% 50 mM TEAB pH 8.0 followed by shaking at 1000 rpm (Eppendorf tabletop) for 2.5 h ...
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bioRxiv - Genomics 2022Quote: ... and the pellet was resuspended in 500 ul Buffer CT and the mixture was transferred to a 2.0 mL Protein LoBind tube (Eppendorf # 022431102). The suspension was spun again at 16,000 x g at 4 C for 2 min and the supernatant discarded ...
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bioRxiv - Cell Biology 2022Quote: ... The fully dried gel slices were incubated with 1 µg trypsin per tube in 50 mM ammonium bicarbonate buffer at 37°C overnight on a ThermoMixer (Eppendorf). Digested peptides were extracted twice with 150 µl of 30% acetonitrile ...
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bioRxiv - Biochemistry 2020Quote: ... frozen leaves were extracted in 50 mM P buffer (pH 7.5) at a ratio of 1:8 (w/v) and centrifuged (Eppendorf 5417R) twice at 18,000g for 20 min ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mL of 200 µM monomeric αSyn was incubated in fibrillation buffer (Supplementary Table S2) in a 1.5 mL Protein LoBind Eppendorf tube (Eppendorf, Germany) on an orbital shaker (PCMT Thermoshaker Grant-bio ...
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bioRxiv - Cancer Biology 2021Quote: ... rinsed with 400 μL ChIP elution buffer (containing 50 μg RNase A) and incubated at 37 °C at 1,200 rpm on a Thermomixer (Eppendorf, Germany) for 1 h followed by incubating with 2 μL Proteinase K (New England Biolabs ...
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Phosphoproteomics of cellular mechanosensing reveals NFATC4 as a regulator of myofibroblast activitybioRxiv - Systems Biology 2023Quote: ... Bound phosphopeptides were eluted 2 times with 30 μl elution buffer (40 % ACN, 15 % NH4OH (25 %, HPLC grade)) each and collected by centrifugation into clean PCR tubes (Eppendorf). Samples were concentrated in a SpeedVac for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: Single hemocytes were sorted directly into a 384-well plate containing 2.3 µl of lysis buffer (Eppendorf twin.tecTM PCR plates) using a CyCloneTM robotic arm and at highly stringent single cell sort settings (single mode ...
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bioRxiv - Molecular Biology 2024Quote: ... though the maximum volume of beads + gDNA + binding buffer should not exceed 1.4 ml for a single 1.5 ml Protein LoBind tube (Eppendorf, Cat. 30108442). Multiple tubes can be used and pooled at the restriction enzyme digest step ...
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bioRxiv - Developmental Biology 2024Quote: ... organoids were treated with 200 µL of probe hybridization buffer for 30 min at 37 ºC inside a Thermomixer covered by the ThermoTop (Eppendorf) to avoid evaporation of the buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins that adhered to the filter were then incubated with 200 µL of 10 mM dithiothreitol in lysis buffer using a ThermoMixer (Eppendorf) at 37°C and 600 rpm for 1 min and then with no agitation for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... cell nuclei were resuspended in 500 μl of 1.2X restriction enzyme buffer and 0.3% SDS (final concentration) and incubated at 37°C with shaking at 300 rpm (Eppendorf Thermomixer). After 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... the DNA was eluted from the beads by incubating with 150μl elution buffer (100mM NaHCO3 and 1% SDS) for 20 mins at 65°C with vortexing using Eppendorf ThermoMixer C (Eppendorf). The supernatant was collected ...
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bioRxiv - Developmental Biology 2024Quote: ... Another 1 ml of Washing buffer B was added and transferred to 1.5 ml Protein lo-bind tubes (Eppendorf, 0030108116) with beads ...
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bioRxiv - Neuroscience 2024Quote: ... 16 adult heads were placed into lysis buffer, homogenised (Kontes, Pellet Pestle Motor) for five minutes and centrifuged (MiniSpin Plus, Eppendorf) for 10 min at 12000 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... The peptides were eluted from the C18 with buffer B (0.1% TFA, 40% AcN) before drying with a Vacufuge plus (Eppendorf 022820109). Dried peptides were reconstituted in 0.1% TFA & 0.5% AcN before loading on a timsTOF Pro 2 operated in DIA-PASEF mode coupled to a NanoElute UHPLC system (Bruker Daltonics) ...
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bioRxiv - Molecular Biology 2024Quote: ... which had been pretreated with HCM-BSA buffer and shaken at 1,500 rpm (mixing radius = 1.5 mm) using the Eppendorf ThermoMixer C (Eppendorf, Hamburg, Germany) for 5 min at 25°C ...
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bioRxiv - Biophysics 2024Quote: ... and eluted by adding 1mL Buffer B to the cartridge and spinning in a swing bucket rotor (Eppendorf 5910 R) at 300rpm for 5 min ...
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bioRxiv - Biophysics 2024Quote: ... and eluted by adding 1mL Buffer B to the cartridge and spinning in a swing bucket rotor (Eppendorf 5910 R) at 300rpm for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... a mix of pre-polymerized mother filaments (formed from 1 μM 10 % AlexaFluor-568-labeled G-actin incubated in F-buffer in an Eppendorf tube for 2 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were digested by suspending the washed tips in 100 μL lysis buffer and were heated at 95 °C for 10 mins with agitation (Thermomixer, Eppendorf). After heating ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Frozen pellets were thawed and resuspended in 0.5 mL assay buffer (see assay descriptions) and transferred to Protein Lobind tubes (Eppendorf, Hamburg) containing 0.25 mL 0.5 mm glass beads ...
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REGN-COV2 antibody cocktail prevents and treats SARS-CoV-2 infection in rhesus macaques and hamstersbioRxiv - Microbiology 2020Quote: ... was added to each sample to assess extraction efficiency RNA extraction was performed using the EpMotion M5073c Liquid Handler (Eppendorf) and the NucleoMag Pathogen kit (Macherey-Nagel) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM label was subsequently added and samples were incubated over night at 4°C and 350 rpm in a Thermomixer (Eppendorf). Excess label was removed by using a Sephadex G25 column (GE Healthcare Bio-Science AB ...
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bioRxiv - Neuroscience 2021Quote: ... Single EGFP+ cells were sorted on a BD Influx equipped with a 140 µm nozzle and a cooling unit with a sample temperature of 4°C and collected into a DNA LoBind tube (Eppendorf) containing aCSF with 0.5% BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... no template control from reverse-transcription and no template control from PCR Endpoint RT-PCR was run together with target samples in a Mastercycler gradient Thermocycler (Eppendorf) under following conditions ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were kept on ice and centrifuged at 14 krpm for 2 min on a mini spin plus centrifuge (Eppendorf) before measurement ...
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bioRxiv - Cell Biology 2021Quote: ... after which the protein samples were denatured at 650 rpm for 1 h at 37°C using a Thermomixer (Eppendorf). Sample reduction and alkylation were individually performed by adding 10 mM dithiothreitol and 40 mM iodoacetoamide ...
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bioRxiv - Plant Biology 2021Quote: ... samples were derivatized with N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (Sigma-Aldrich, 1.0 mg per sample, pH 4.0, 60 min, 37 °C under continuous shaking in an Eppendorf thermomixer) and analysed using a UPLC-MS/MS equipped with an electrospray interface in positive mode (ACQUITY ...
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bioRxiv - Microbiology 2021Quote: ... 0.5-1 mL culture sample was harvested by centrifugation for 3 min at 13806 rpm (Centrifuge 5424, FA-45-24-11, Eppendorf) and resuspended in 100-500 µL 10 mM NaOH depending on the size of the cell pellet ...
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bioRxiv - Cell Biology 2021Quote: ... The resuspended cilia sample was centrifuged at 16,000g for 10 min at 4°C in a microfuge (Eppendorf, Centrifuge 5415D). The supernatant was discarded ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were spun at 10,000 g for 15 min (spin 2 in the same Eppendorf FA-45-48-11 rotor) and the resulting supernatant is the tissue lysate (TL ...
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bioRxiv - Neuroscience 2020Quote: ... and RNA concentration was measured in a 1 μl sample diluted 1:200 in nuclease-free water in a spectrophotometer (BioPhotometer, Eppendorf). cDNA was synthesized using 1 μg of input RNA with an iScript™ cDNA Synthesis Kit and MyIQ thermocycler (both Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... Sarkospin solubilization was then performed by incubating the samples at 37 °C under constant shaking at 600 rpm (Thermomixer, Eppendorf) for 45 min ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The cDNA of liver samples of each treatment were amplified in duplicate using a Mastercycler®ep realplex system (Eppendorf) in 96-well optical white plates ...
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bioRxiv - Genetics 2022Quote: ... The samples were then incubated at room temperature (about 25°C) at 450 rpm for 20 mins on Thermomixer (Eppendorf). Putting the samples on magnetic stand to facilitate removing supernatant ...
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bioRxiv - Synthetic Biology 2021Quote: ... The chip is then loaded onto a 10X Chromium machine for droplet generation and samples were transferred onto a pre-chilled strip tube (Eppendorf), and reverse transcription was performed using a 96-well thermal cycler (Thermo Fisher) ...
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bioRxiv - Biophysics 2021Quote: ... and the aggregated samples were pelleted via centrifugation at 20.000 g for 10 min (Eppendorf 5417 R table-top centrifuge). The pellets were washed with 500 μL ice-cold acetone and centrifuged for 5 min ...
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bioRxiv - Genetics 2021Quote: ... Oxford Nanopore Technologies, SQK-LSK109. At this step, the resuspendend beads from the three samples were pooled into one Eppendorf tube ...
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bioRxiv - Cancer Biology 2021Quote: ... Each sample was processed on an independent Chromium Single Cell A Chip (10X Genomics) and run on a thermocycler (Eppendorf). 5’ gene expression libraries were sequenced using NextSeq 500 150-cycle high-output flow cells or NovaSeq 6000 200 cycle S4 flow cells ...
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bioRxiv - Neuroscience 2023Quote: ... Samples that did not meet the minimum 60 ng/uL were concentrated using an Eppendorf Concentrator Plus (Eppendorf, Hamburg, Germany) for 10 minutes at 45°C ...
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bioRxiv - Genetics 2023Quote: ... Samples were mixed at 850 rpm for 1 hour at room temperature on a 96 well MixMate plate mixer (Eppendorf). The beads were concentrated using a magnet and the supernatants removed ...
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bioRxiv - Neuroscience 2023Quote: ... After centrifuging the samples for 15 minutes at 5°C at 4000 rpm on bench top centrifuge 5810R (Eppendorf, USA), 600 μL of dichloromethane (bottom layer ...