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Citations for Eppendorf :
451 - 500 of 621 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... samples were centrifuged at 20,000× g for 30 min at 4 °C (5804/5804 R Centrifuge, Eppendorf, USA), and supernatants were discarded ...
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bioRxiv - Immunology 2023Quote: ... samples were sorted into PBS with 0.04% BSA (w/v) in 1.5 mL Protein LoBind tubes (Eppendorf, 0030108442). For ATAC-seq ...
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bioRxiv - Immunology 2023Quote: ... the sample was briefly vortexed and incubated at 56°C for 10 minutes with a Thermomixer C (Eppendorf) at 1000 rpm ...
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bioRxiv - Microbiology 2023Quote: ... A 1 mL sample was centrifuged for five minutes at 10,000 rpm (Eppendorf MiniSpin F-45-12-11), resuspended in 1 mL phosphate buffered saline (PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were digested for 1 hour at 37°C with constant low agitation using a thermomixer (Eppendorf). The digested cells were pelleted and washed twice with RPMI media in the presence of 5% FBS ...
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Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... A 1.5 ml sample was centrifuged for five minutes at 8,000 rpm (Eppendorf MiniSpin F-45-12-11), resuspended in 1 ml phosphate buffered saline (PBS) ...
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bioRxiv - Genomics 2022Quote: ... Then 14.6ul of each ligated DNA sample was aliquoted into a 1.5ml DNA lo-bind tube (Eppendorf, Z666548). All reagents and master mixes were kept on ice throughout this protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Wholemeal flour samples were weighed (6 mg) and transferred into a deep well plate (96/1000 μL, Eppendorf). Phosphate buffered saline (600 μL ...
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bioRxiv - Biochemistry 2023Quote: ... Two extracts from the same sample were combined and dried by Vacufuge Concentrator System (Eppendorf 5301, Eppendorf, Germany).
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bioRxiv - Biochemistry 2023Quote: ... Two extracts from the same sample were combined and dried by Vacufuge Concentrator System (Eppendorf 5301, Eppendorf, Germany).
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bioRxiv - Cell Biology 2022Quote: ... samples were incubated at 37°C for 30 min with shaking at 300 rpm in ThermoMixer F1.5 (Eppendorf). The beads were removed by centrifuge at 16000 xg for 5 min at 4°C and placing on a magnetic stand ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were centrifuged at 4000 rpm × 5 min at 4°C in a pre-cooled microcentrifuge (Eppendorf 5415R) and the supernatant was collected ...
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bioRxiv - Biophysics 2023Quote: ... MC samples were loaded on the lower plate with a volume of 210 μl with a micropipette (Eppendorf Research® plus 1000 μl ...
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bioRxiv - Physiology 2023Quote: ... We made some adjustments to the original protocol: samples were automatically pipetted (epMotion® 5070, Eppendorf, Hamburg, Germany) in duplicates in 384-qPCR plates (n = 5 plates ...
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bioRxiv - Plant Biology 2023Quote: ... The protein samples were centrifuged at maximum speed for 1 min in an Eppendorf™ 5424 Microcentrifuge (Eppendorf). The proteins were separated on 10% SDS polyacrylamide gels and transferred onto Immuno-Blot PVDF membrane (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were centrifugated at 10.000xg at room temperature for 5 min (centrifuge MiniSpin® Eppendorf, ClearLine, Venice, Italy) and stored at −80°C until further use.
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were then extracted for 3 hr at 4°C in a ThermoMixer (Eppendorf US, Enfield, CT, USA), followed by evaporation of the methanol component with a speedvac concentrator (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were centrifuged for 5 min at 25001 relative centrifugal force (Centrifuge 5427 R, Eppendorf; Hamburg, Germany). Every sample was diluted 1:100 in 1% HNO3.
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bioRxiv - Microbiology 2024Quote: ... The collected sample was concentrated in a SpeedVac (45°C, V-AQ, 1-2 hrs) (Eppendorf Concentrator 5301). The samples were stored at 20°C until LC-MS measurement.
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bioRxiv - Developmental Biology 2020Quote: ... 300-400 female G10011 pupae (at 70-80% pupal development) were injected with dsRNA (2μg /μl) or injection buffer only (control) using a FemtoJet Express (Eppendorf). Injected pupae were placed on fine wheat flour (type 405 ...
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bioRxiv - Molecular Biology 2021Quote: ... and re-dissolved in lysis buffer supplemented with 1% v/v SDS at RT in a Thermomixer R (Eppendorf) at 1200 rpm for 1-2 hours ...
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bioRxiv - Biochemistry 2021Quote: ... Dynabeads were separated magnetically and elution buffer containing biotinylated proteins was transferred to a new 1.5 ml LoBind protein tube (Eppendorf). 70 µl of sample was electrophoresed on a NuPAGETM 4-12% Bis-Tris protein gel (Invitrogen) ...
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bioRxiv - Genetics 2020Quote: ... buffer (10mM Tris-HCl pH 8.0, 0.5mM EDTA, 1M NaCl) in a 1.5ml Eppendorf Lobind tube (Eppendorf, 0030 108.051). DNA binding was performed in a thermo mixer (Eppendorf ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were eluted with 60 μL of buffer B (80% ACN, 0.1% formic acid in H20) and reduced in a Vacufuge plus (Eppendorf) to a final volume of 3 μL ...
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bioRxiv - Microbiology 2020Quote: ... the microbial pellet was resuspended in 500 μL lysis buffer (4% SDS (w/v) in 50 mM Tris-HCl buffer pH 8.0) followed by incubation in a Thermomixer Comfort (Eppendorf) at 95 °C for 10 min with agitation ...
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bioRxiv - Molecular Biology 2023Quote: ... beads were washed with minimal washing buffer (50 mM Tris, pH 7.5; 150 mM NaCl) for 15 min and transferred to Protein LoBind tubes (Eppendorf). The washing procedure was repeated twice and beads were again transferred to new Protein LoBind tubes ...
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bioRxiv - Developmental Biology 2023Quote: ... all isolated fresh blastomeres or cells were washed three times with CUT&RUN wash buffer to avoid possible contamination and then transferred into a 1.5mL low-binding PCR tube (Eppendorf) containing 100µL of the wash buffer ...
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bioRxiv - Genomics 2023Quote: ... Beads were then resuspended in 200 μL IP Elution Buffer (1% SDS, 0.1 M NaHCO3) and incubated at 65 °C in a Thermomixer (Eppendorf) with interval mixing to dissociate antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... we further purified the extracts by treatment with 1% sodium dodecyl sulfate in NaP-EDTA buffer and centrifugation at 13,000 rpm for 10 min (rotor FA-24×2, Eppendorf). This purification process was repeated two times ...
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bioRxiv - Molecular Biology 2022Quote: ... Elution buffer incubation time was extended to ~20 minutes and DNA was eluted into LoBind tubes (Eppendorf, Hamburg, Germany).
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bioRxiv - Genomics 2024Quote: ... The supernatant was removed and cells were resuspended in 2×100µl F-buffer in a 1.5mL low binding tube (Eppendorf, 022431021). Cells were counted and Freezing Buffer (F ...
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bioRxiv - Biochemistry 2024Quote: ... were prewashed 3 times with 400 μL of lysis buffer and resuspended in 200 μL lysis buffer in 1.5 mL tube or each well of 96-deepwell plate (951032603, Eppendorf). SA-Mag beads were separated from supernatant using DynaMag™-2 magnet (12321D ...
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bioRxiv - Microbiology 2021Quote: ... with 100 μl aliquot of the ultra-low gelling temperature agarose gel solution (6% in Mueller Hinton Broth II, cation-adjusted) to 85 °C in a Thermomixer (Eppendorf Thermomixer R Shaker, Eppendorf). Once the temperature was reached ...
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bioRxiv - Biophysics 2021Quote: ... small volumes of a 50 nM DNA sensor solution in Phenol red free medium was microinjected into the cells using a Transjector 5246 (Eppendorf, Hamburg, Ger, 5246 01084) in combination with Femtotips (Eppendorf ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then analyzed by centrifugation at 13,000 rpm for 10 min (Eppendorf table top centrifuge, Eppendorf, Hamburg, Germany) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... The extraction step incorporated a 2-fold concentration of saliva samples using a vacuum concentrator (Concentrator plus, Eppendorf, Germany) resulting in a detection sensitivity of 3pg/ml ...
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bioRxiv - Synthetic Biology 2020Quote: ... Samples were subsequently incubated at 25°C for 1 hour with shaking 1000 rpm (Thermo Mixer C, Eppendorf, Germany) in order to fluorescently label EVs ...
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bioRxiv - Systems Biology 2021Quote: ... OD600 was measured at each sampling time using a diluted volume of sample and a Biophotometer spectrophotometer (Eppendorf, Germany). CFUs were determined using a 100-200 μl of a diluted volume of samples plated in YPD solid medium (2% glucose ...
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bioRxiv - Microbiology 2021Quote: ... Organ samples were weighed and triturated in 1 ml sterile tissue culture media using a tissue homogenizer (Eppendorf, Germany). Two hundred microliters of the tissue homogenate/ swab specimens were used for RNA extraction using MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit as per the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... OD600 was measured at each sampling time using a diluted volume of sample and a Biophotometer spectrophotometer (Eppendorf, Germany). CFUs were determined using a 100-200 μl of a diluted volume of samples plated in YPD solid medium (2% glucose ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant of all samples was then discarded and remaining precipitate was put into small 1.5 ml Eppendorf tubes (Eppendorf Safe-Lock Tubes ...
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bioRxiv - Genomics 2020Quote: ... From each sample 50 ul of eluate was used as input and concentrated using the SpeedVac vacuum concentrator (Eppendorf). Samples were dissolved in 10 μl of master mix for fragmentation (consisting of NEB next First Strand Synthesis ...
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bioRxiv - Cell Biology 2022Quote: ... 100μL of RNAse free water with RNAsin (40 U/mL) was added and samples incubated in a thermomixer (Eppendorf) at 300 rpm for at 55°C for 1h for decrosslinking ...
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bioRxiv - Genomics 2023Quote: ... the sample in each 96-well plate was pooled into 12 DNA low-binding 1.5 ml tubes (Eppendorf, 022431021). Genomic DNA (gDNA ...
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bioRxiv - Bioengineering 2023Quote: ... these samples were centrifuged for 6 min at 16,740 x g at room temperature (5427 R, Eppendorf, Hamburg, Germany), and the supernatant was discarded ...
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bioRxiv - Biochemistry 2023Quote: 20 µg of dry protein sample was dissolved in 40 µL 25% TFA in a 1.5mL low-binding polypropylene vial (Eppendorf). The vial was sealed with a micro tube cap lock (Scientific Specialties ...
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bioRxiv - Plant Biology 2023Quote: ... samples (stored for 4-12 weeks) were thawed at room temperature for 15 mins and centrifuged (Eppendorf Centrifuge 5417C) at 11,000 rpm for 14 mins to extract bulk leaf sap ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was incubated at 25 °C for 2 h with intermittent mixing (Thermomixer comfort, Eppendorf, Wesseling-Berzdorf, Germany) to reduce viscosity ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and the samples were incubated overnight (55 °C at 1100 rpm) in a thermomixer (model 5350; Eppendorf, Hauppauge, NY). After incubation ...
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bioRxiv - Molecular Biology 2024Quote: ... 80 μl samples were used in low-volume disposable cuvettes with 1 cm path-length (UVette, Eppendorf Austria GmbH). The time-dependent autocorrelation function was measured for 10 s ...