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Citations for Omega Bio-Tek :
701 - 750 of 755 citations for Recombinant Human TEK His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2019Quote: ... Three replicates of the PCR reactions for each sample were combined and the PCR products were purified using Gel Extraction Kit (Omega Bio-Tek, USA). DNA was quantified using Qubit@ 2.0 Fluorometer (Thermo Scientific) ...
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bioRxiv - Zoology 2020Quote: Whole genomic DNA was extracted from either the whole specimen or pereopods 5–7 using an EZNA Tissue Kit (Omega Bio-tek Inc.) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... The pellet of synchronized worms (L2) was treated with 4 mm steel beads and 1 mL of RNA-Solv® Reagent (Omega Bio-Tek). The mix was vortexed for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... The subsequent step of PCR purification was carried out using the Mag-Bind® Total Pure NGS beads (OMEGA Bio-Tek, Georgia, USA), retaining fragments >100 bp ...
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bioRxiv - Genomics 2021Quote: Genomic DNA (gDNA) was isolated using the KingFisher Flex Purification System with the Mag-Bind® Blood & Tissue DNA HDQ Kit (Omega Bio-Tek). The gDNA concentrations were quantitated by Qubit ...
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bioRxiv - Genomics 2021Quote: ... Barcodes were ligated to the end-repaired DNA and purified with 0.1× or 0.15× beads (Omega Bio-Tek Inc, catalog no. M1378-01), resuspended in a custom buffer (10 mM Tris-Cl pH 8.0 ...
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bioRxiv - Systems Biology 2022Quote: ... the membrane was collected to extract bacterial DNA genome with an E.Z.N.A™ Mag-Bind Soil DNA Kit (OMEGA Bio-Tek, Inc., GA, USA), according to the manufacturers’ instructions ...
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bioRxiv - Pathology 2021Quote: The total genomic DNA was isolated from digestive gland samples using the E.Z.N.A.® soil DNA kit (Omega Bio-tek, Norcross, GA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: DNA was extracted from 0.2 g of dried young leaf tissue with the E-Z 96 Plant DNA Kit (Omega Bio-Tek, Norcross, GA) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from 5 mg of spleen lysate using the Omega Biotek Total RNA 96 kit (Omega Bio-Tek, Norcross, GA) and a KingFisher Flex Instrument (ThermoFisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted with a commercial kit following the manufacturer’s recommendations (Mag-Bind® Plant DNA DS, Omega Bio-Tek, Norcross, GA, USA), followed by sample preparation according to method developed by Elshire et al ...
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bioRxiv - Evolutionary Biology 2022Quote: ... were scraped off the plate and DNA was extracted using the E.Z.N.A.® Fungal HP DNA Kit (Omega Bio-Tek, Norcross, GA, USA) according to the DNA extraction procedure explained in Peris et al ...
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bioRxiv - Microbiology 2022Quote: Total RNA extraction from the mycelial samples was conducted with the E.Z.N.A.® Plant RNA kit (Omega Bio-Tek Inc. Norcross, GA, USA) according to the manufacturer’s extraction protocol with minor modifications ...
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bioRxiv - Plant Biology 2022Quote: ... Plants were confirmed to be homozygous mutants by genomic DNA extraction from two green rosette leaves using the EZNA Plant DNA kit (Omega Bio-Tek, D3485) followed by PCR genotyping using standard Taq DNA polymerase (18038-042 ...
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bioRxiv - Cell Biology 2024Quote: DNA was extracted from cell suspensions using the Mag-Bind® Blood & Tissue DNA HDQ extraction kit (Omega Bio-Tek, Nocross, Georgia, USA) according to manufacturer’s specifications in the Mag-Bind® Blood protocol ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted and purified from whole blood samples or filter paper employing the E.Z.N.A.® Blood DNA mini-Kit (Omega Bio-Tek ®, Georgia, USA) following manufacturer instructions with minor modifications (addition of TEN buffer [20 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: The genomic DNA was extracted from microbial samples (a total of 16 culture samples and 62 samples of mice fecal contents) using the E.Z.N.A.® soil DNA Kit (Omega Bio-tek, Norcross, GA, U.S.) in accordance with the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: Genomic DNA (gDNA) was isolated using the KingFisher Flex Purification System with the Mag-Bind® Blood & Tissue DNA HDQ Kit (Omega Bio-Tek). For smaller cell pellets from sorted cell populations ...
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bioRxiv - Genomics 2023Quote: Genomic DNA (gDNA) was isolated using the KingFisher Flex Purification System with the Mag-Bind® Blood & Tissue DNA HDQ Kit (Omega Bio-Tek). The gDNA concentrations were quantitated by Qubit.
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bioRxiv - Microbiology 2023Quote: Total genomic DNA was extracted from all the collected samples using the E.Z.N.A.® Soil DNA kit (Omega Bio-tek, Norcross, GA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated according to the tissue protocol of the Mag-Bind® Blood & Tissue DNA 96 Kit (#M6399-01, Omega Bio-tek) and quantified using the Quantifluor® dsDNA System (#E2670 ...
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bioRxiv - Biochemistry 2023Quote: Barcoded NGS libraries for each sorted population were generated using a two-step PCR protocol using AmpliTaq Gold (Invitrogen. The resulting PCR products were purified using Mag-Bind® TotalPure NGS beads (Omega Bio-tek) and amplified in a second PCR introducing the standard Illumina adapters ...
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bioRxiv - Synthetic Biology 2023Quote: The pDNA production obtained with the different strains was evaluated by extracting the pDNA with the MicroElute Gel Extraction Kit (Omega Bio-Tek, Inc.) and quantifying it with Qubit Invitrogen (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: Genomic DNA was extracted from a total of 1,050 rhizosphere samples derived from 175 genotypes at two field locations using the E.Z.N.A.® soil DNA Kit (Omega Bio-tek, Norcross, GA, U.S.) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2024Quote: ... Indexed amplicons were pooled in a final library and purified with the Mag-Bind Total Pure NGS kit (Omega Bio-Tek, Norcross, GA). The pooled library was quantified using the Qubit dsDNA High Sensitivity kit (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: Microbial DNA was extracted from the Shan Partridge duck samples using the E.Z.N.A.® Stool DNA Kit (Omega Bio-tek, Norcross, GA, U.S.) in accordance with the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2019Quote: Plasmid DNA was purified using with either E.Z.N.A.® Endo Free Plasmid Mini Kit II or Midi Kit (Omega Bio-Tek D6950-01, D6915-03) and packaged into lentiviral vectors with psPAX2 (Addgene #12260 ...
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bioRxiv - Immunology 2019Quote: ... the aqueous layer was mixed with an equal volume of cold 75% ethanol and transferred to MicroElute LE RNA Columns (Omega Bio-Tek, Norcross, GA). After RNA extraction ...
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bioRxiv - Plant Biology 2021Quote: DNA extraction from leaf tissue was carried out using the Omega E-Z 96 Plant DNA Kit (Omega Bio-tek, Inc. Norcross, GA, USA). Samples were then genotyped at Michigan State University using an apple 20K Infinium® SNP array (Bianco et al. ...
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bioRxiv - Plant Biology 2020Quote: ... Samples were ground in LN2 to a fine powder before RNA extraction including optional DNase treatment was performed following the manufacturers standard protocol provided with the E.Z.N.A® Plant RNA Kit (Omega Bio-tek Inc., http://omegabiotek.com/store/).
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bioRxiv - Microbiology 2022Quote: ... Final elution was standardized across all DNA extraction methods with a volume of 100 μl in non-EDTA 10 mM tris-HCL elution buffer EDTA is known to interfere with downstream sequencing (Omega Bio-Tek, Norcross USA) (pH 8.5 and at 70 °C ...
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bioRxiv - Bioengineering 2019Quote: Genomic DNA from ear tissue of founders (F0) pigs was isolated according to the protocol of the OMEGA Kit (OMEGA Bio-Tek, Georgia, USA) for PCR and southern blot analysis ...
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bioRxiv - Plant Biology 2021Quote: ... Genomic DNA (gDNA) was extracted from powdered leaf samples using the E-Z 96® Plant DNA Kit (Omega Bio-Tek, Norcross, GA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The remaining 45 μL from each triplicate PCRs were pooled and purified using Mag-Bind® RXNPure plus (Omega Bio-tek; Norcross, GA, USA). The clean amplicons from each sample were pooled at equal concentrations ...
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bioRxiv - Molecular Biology 2022Quote: PCR replicates for each sample were pooled prior to purification with Mag-Bind® Total Pure NGS Beads (Omega Bio-tek Inc., GA, USA), following the double size selection protocol established by Bronner et al ...
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bioRxiv - Microbiology 2024Quote: ... Then the DNA of the gut microbial samples from goats in the SSMF and FMMF groups of Nanjiang yellow goats was extracted using the OMEGA stool DNA kit (Omega Bio-Tek, the U.S). Then the quality of the DNA was assessed using 1.5% agarose gel electrophoresis ...
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bioRxiv - Immunology 2024Quote: ... Transfection reactions were carried out in 100-mm cell culture dishes with the plasmid DNA (20 μg) purified with OMEGA Plasmid Maxi Kit (Omega Bio-Tek, Norcross, GA) and 40 μl of Lipofectamine 2000 reagent (Life Technologies) ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from the samples using RNA-Solv® Reagent following the manufacturer’s instructions (Omega Bio-Tek-R6830-02, Norcross, GA, USA) and subsequently stored at -80 °C to ensure its stability ...
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bioRxiv - Genomics 2023Quote: ... DNA was amplified by PCR with Phusion HF 2X MM and Nextera universal barcodes described previously and was purified by two-sided SPRI purification (Omega Bio-Tek, M1378-01). Samples were sequenced using a NovaSeq SP kit (Illumina ...
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bioRxiv - Evolutionary Biology 2023Quote: ... was produced in DH10B cells and the plasmid purified from single-bacterial colonies using the E.Z.N.A.® Plasmid Mini Kit I (Omega Bio-tek, cat. no. D6942-01). NLFK cells were transfected using Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We used up to 30 mg of each of the 421 preserved fence lizard tissue samples for E.Z.N.A.® tissue kit (D3396, Omega Bio-Tek, Inc., Norcross, GA, USA) DNA extractions ...
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bioRxiv - Bioengineering 2024Quote: Plasmid DNA used to generate lentivirus for cell line engineering was prepared using a polyethylene glycol precipitation protocol.37 Plasmid DNA used for transient transfection of HEK293FT cells was prepared using a MidiPrep protocol (Omega Bio-Tek, D6904-04). DNA purity and concentrations for relevant experiments were measured with a NanoDrop 2000 (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... the PCR products were evaluated by electrophoresis in 1.5% agarose gel and purified with the Mag-Bind RxnPure Plus kit (Omega Bio-Tek, CA, Norcross, GA, USA) by mixing 20 μL of amplicon with 25 μL of Mag-Bind in a 96 well flat-bottom micro-titer plate ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was obtained from all BEC lines (3 Ctrl BEC and 5 SZP BEC) by phenol-chloroform extraction using RNAsolv (Omega Bio-Tek, Norcross, GA, USA). 1 μg of RNA was treated with DNase I (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... and the pelleted material was resuspended in 1-2 mL of supernatant and transferred to a microcentrifuge tube for DNA extraction using the E.Z.N.A® Bacterial DNA Kit (Omega Bio-tek, Inc. Norcross, GA, United States). The manufacturer’s Centrifugation Protocol was used with minor modifications ...
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bioRxiv - Genetics 2020Quote: Genomic DNA (gDNA) was isolated using either the KingFisher Flex Purification System with the Mag-Bind® Blood & Tissue DNA HDQ Kit (Omega Bio-Tek #M6399-01), or the Machery Nagel NucleoSpin Blood Maxi (2e7-1e8 cells) ...
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bioRxiv - Immunology 2022Quote: The expression plasmids of Rtx variants were constructed according to the previous report30 and prepared using the Endo-Free Plasmid Maxi Kit (Omega Bio-Tek, Norcross, GA, USA). HEK293E cells were seeded at 5E5 cells/mL in SMM 293-TII Expression Medium and adjusted to 1E6 cells/mL 24 h later ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was extracted from a 300 µl aliquot of whole blood using the Mag-Bind Blood and Tissue Kit (Omega Bio-Tek Inc., Norcross, GA). DNA concentration was evaluated with Picogreen and Victor X2 fluorometry (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... RNA from each sample was DNase treated using Turbo DNase and cleaned up with Mag-Bind® TotalPure NGS beads (Omega Bio-Tek M1378-00). First-strand cDNA synthesis was performed using SuperScript III Reverse Transcriptase and a primer that binds to the conserved VSG 14-mer in the 3′-UTR (5’-GTGTTAAAATATATC-3’) ...
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bioRxiv - Microbiology 2023Quote: The rust layer biofilm and seawater samples were subjected to DNA isolation using the Bacterial DNA Isolation Kit (Mobio, Carlsbad, CA, USA) and Water DNA Isolation Kit (Omega Bio-tek, Doraville, GA, USA), respectively ...