Labshake search
Citations for Omega Bio-Tek :
601 - 650 of 705 citations for Recombinant Human TEK His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... We extracted total genomic DNA from fecal samples utilizing the E.Z.N.A.® Stool DNA Kit (Omega Bio-tek Inc.; Norcross, GA), following the manufacturer protocols ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were cleaned with Mag-Bind® TotalPure NGS beads (0.6x beads/sample ratio, Omega Bio-tek, Norcross, GA, USA) following the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA of 80 specimens was extracted from gill tissue snips with the E.Z.N.A Mollusc DNA Kit (Omega Bio-tek, Norcross, GA) at the University of Rhode Island and sent for plexWell384 library preparation and NovaSeq6000 2x150 bp sequencing at Psomagen ...
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bioRxiv - Plant Biology 2023Quote: ... DNA was isolated from leaves and seeds using the E-Z 384 Plant DNA HT kit (Omega Bio-tek, GA, USA).
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from 100 mg of grounded leaf tissue using the E.Z.N.A Plant Mini Kit (Omega Bio-tek, Norcross, USA) and contaminating DNA was removed using turbo DNAfree DNAse I (Ambion ...
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bioRxiv - Zoology 2023Quote: ... genomic DNA was extracted from cell pellets using the Mag-Bind Blood and Tissue Kit (Omega Bio-Tek Inc., Norcross, GA). DNA concentrations were assessed using Picogreen and Victor x2 fluorometry (Life Technologies ...
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bioRxiv - Plant Biology 2024Quote: ... Frozen root samples were ground in liquid nitrogen and total RNAs were extracted using E.Z.N.A Total RNA Kit I (Omega Bio-tek, Georgia, USA). RNA quantity and purity were evaluated with a Qubit®2.0 fluorometer (Invitrogen™ ...
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bioRxiv - Molecular Biology 2024Quote: ... the genomic DNA of gill tissues was first extracted using a mollusk DNA extraction kit (Omega Bio-tek, Norcross, GA, USA) for the qRT-PCR template ...
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bioRxiv - Immunology 2021Quote: The mixed intestinal tissues were subjected to total DNA extraction using an E.Z.N.A.® soil kit (Omega Bio-Tek, Norcross, GA, USA). DNA concentration and purity were assayed using NanoDrop2000 ...
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bioRxiv - Developmental Biology 2021Quote: ... 4th and 6th instars of queens and workers as extracted in accordance with the standard protocol of the of the Total RNA Kit I (R6834, Omega Bio-tek), respectively ...
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bioRxiv - Plant Biology 2019Quote: ... Pooled samples were amplified using 18 cycles of PCR and size-selection was performed using 0.4× and 0.8× volumes of Mag-Bind Total Pure NGS magnetic beads (Omega Bio-Tek, Georgia, USA) to remove fragments larger than 1.5 kb and smaller than 121 bp ...
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bioRxiv - Genetics 2019Quote: ... Genomic DNA from each individual was purified using the E-Z 96 Tissue DNA Kit (Omega Bio-Tek, Inc., Norcross, GA, USA). DNA was quantitated with the Quant-iT™ PicoGreen® Assay (Invitrogen ...
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bioRxiv - Genomics 2019Quote: ... Genomic DNA was extracted from immature leaf tissue using the E-Z 96 Plant DNA kit (Omega Bio-Tek, Norcross, GA, USA) with Proteinase K was added to the initial buffer ...
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bioRxiv - Microbiology 2020Quote: Nasal swabs where taken from two adult healthy volunteers using STX 764 sterile small polyester swabs (Texwipe) and immediately stirred for 30 seconds in 1 ml of TRK lysis buffer (Omega Bio-tek). One volume of 70% EtOH was added ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted from GBM-DCs and TRA-1-81+/SSEA4+ ic-GSCs using the EZNA Total RNA Kit (R6834-01 Omega Bio-Tek). Quality control ...
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bioRxiv - Biophysics 2021Quote: ... Near 1250 bp regions of double-stranded products of protected digestion of rolling circle amplification were inserted into modified pET14b backbones were selected by agarose-gel-cutting and purified by Omega Bio-Tek E.Z.N.A ...
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bioRxiv - Molecular Biology 2022Quote: ... 2013) with a ratio of 0.9X and 0.15X of magnetic beads (Mag-Bind® RXNPure Plus, Omega Bio-tek Inc, GA, USA) to sub-library ...
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bioRxiv - Genomics 2022Quote: ... DNA was size-selected following shearing using a 0.55x followed by a 0.70x double-SPRI magnetic bead purification (Omega Bio-Tek, M1378-01). At this point ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plasmids were transformed into DH5α electrocompetent cells for cloning and purification (E.Z.N.A.® Plasmid DNA Midi Kit, Omega Bio-tek, Norcross, GA) for sequencing and cell-free reaction ...
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bioRxiv - Microbiology 2022Quote: Microbial genomic DNA extraction from soil samples was performed with the E.Z.N.A.® Soil DNA Kit (Omega Bio-tek, Norcross, GA, USA). DNA quality assays were performed on 1% agarose gels ...
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bioRxiv - Zoology 2022Quote: ... the membrane was stored at −80 °C for extraction of bacterial DNA genome with an E.Z.N.ATM Mag-Bind Soil DNA Kit (OMEGA Bio-Tek, Inc., GA, USA), according to the manufacturers’ instructions.
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bioRxiv - Molecular Biology 2019Quote: ... and the protein was eluted with six column volumes of 0-100% gradient of elution buffer (binding buffer with 500 mM imidazole) and 2 ml fractions were collected into 96 deep well plates (Omega Bio-tek). The collected fractions were run on SDS-PAGE gel and the fractions corresponding to His10-SUMO-RelA with the least nucleic acid contamination was collected (≈5 ml ...
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bioRxiv - Synthetic Biology 2020Quote: ... T7 RNA polymerase was prepared as previously described.(Swartz et al., 2004) Plasmids were extracted using a Plasmid Miniprep Kit (Omega Bio-Tek). All DNA oligonucleotides were purchased from Integrated DNA Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Total DNA was extracted from plant tissues with the OMEGA Mag-Bind Plant DNA Plus kit (Omega Bio-Tek, Norcross, GA, USA) following the manufacturer’s instructions with the aid of a KingFisher Flex™ liquid handling machine (ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... all products were run on a 2 w/v% agarose gel to verify successful amplification of target and then purified using a PCR purification kit (Omega Bio-Tek). The prepared linear DNA was either directly used in cell-free and cell-free ATPS reactions or used as a template for in vitro transcription.
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bioRxiv - Molecular Biology 2020Quote: ... 2013) with a ratio of 0.9X and 0.15X of magnetic beads (Mag-Bind® RXNPure Plus, Omega Bio-tek Inc, GA, USA) to PCR products ...
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bioRxiv - Microbiology 2020Quote: ... The microbial DNA of fifteen soil samples was extracted from 1.0 g of each sample by the E.Z.N.A.® Soil DNA Kit (Omega Bio-tek, Norcross, GA, U.S.) conforming to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Beads were collected on the magnet and liquid transferred to a new PCR tube in which it was cleaned up twice using Mag-Bind TotalPure NGS beads (Cat. #M1327, Omega Bio-Tek) at 2X ...
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bioRxiv - Cell Biology 2022Quote: ... PCR products were fluorescently labeled at the 5′ ends with Cy5 (Yingjun Corp. China) and purified by gel extraction (OMEGA Bio-TEK). Fluorescently-labeled DNA was then detected using a Biophotometer Plus (Eppendorf ...
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bioRxiv - Molecular Biology 2022Quote: Each of the targeted PCR amplicons obtained from the aforementioned genomic DNA templates was recovered from agarose gels using a Gel Extraction Kit (Omega Bio-Tek) and purified using a Universal DNA Purification kit (TIANGEN ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid was extracted and purified the following morning using the E.Z.N.A.® Plasmid DNA Mini Kit I Q-spin (Omega Bio-tek, Norcross, GA) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... Entire mosquitoes or dissected tissues (ovaries, midgut, head, thorax, rest of the body) were homogenized in 300 μl RNA-Solv reagent (Omega Bio-Tek) using a Precellys 24 homogenizer (Bertin technologies) ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from exposed filters as well as three unexposed controls using the E.Z.N.A Soil DNA Kit (Omega Bio-tek, Norcross, GA, USA). The filters were transferred to disruptor tubes and 800 µL SLX-Mlus buffer was added ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products of these flanks and pKOR1 backbone were purified using the EZNA Cycle Pure Kit (Omega Bio-tek, CA, USA) and Gibson assembled ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted from 250uL of the buffy coat fraction of EDTA-treated blood using the E.Z.N.A Tissue DNA kit (Omega Bio-Tek, Norcross, GA, USA) according to the manufacturer’s protocol except 50 μL of elution buffer was used ...
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bioRxiv - Microbiology 2022Quote: ... genomic DNA was sheared to 300 bp using the Covaris LE220-Plus and size selected with SPRI using TotalPure NGS beads (Omega Bio-tek). The fragments were treated with end-repair ...
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bioRxiv - Molecular Biology 2023Quote: ... coli cells by electroporation and plasmids were extracted using an E.Z.N.A.® Plasmid DNA Mini Kit I (Omega Bio-tek, Georgia, USA). Correct assemblies were confirmed by sequencing in association with the Massey Genome Service (Palmerston North ...
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bioRxiv - Genomics 2022Quote: ... and the reporter plasmids were purified using the E.Z.N.A.® Endo-Free Plasmid Maxi Kit (Catalog No. D6926, Omega Bio-tek, Norcross, GA). The bacterial endotoxin of the input plasmid was removed by adding 5 mol/L NaCl ...
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bioRxiv - Plant Biology 2023Quote: 45-50 inflorescence meristems for each of three biological replicates of Col-0 and clv3-9 were collected and total RNA isolated using the EZNA Plant RNA kit (Omega Bio-tek). RNA was treated with RNase-free DNase (Omega Bio-tek ...
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bioRxiv - Microbiology 2023Quote: ... dissected body parts or tissues were homogenized in either 200 µl DMEM medium for end-point titration on BHK-21 cells or in 1 mL RNA-Solv reagent (Omega Bio-Tek) for RNA isolation ...
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bioRxiv - Biochemistry 2023Quote: ... the samples were diluted 500 times and filtered using the DNA Mini columns with a nucleic acid-affinity membrane (Omega Bio-tek.) to decrease or remove the nucleic acid in samples ...
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bioRxiv - Genomics 2023Quote: ... we have improved the results by purifying the extracted DNA using Omega Mag-Bind TotalPure NGS Beads (Omega Bio-Tek, Norcross, GA), which helps to preserve the life span of the flow cell.
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bioRxiv - Plant Biology 2023Quote: ... Disk samples around infection site were taken and DNA extraction was performed using an E.Z.N.A.® Stool DNA Kit (Omega Bio-Tek, Norcross, USA). For the quantification of DNAs standard curves were prepared using primers designed as follows ...
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bioRxiv - Synthetic Biology 2023Quote: ... The reaction mixture was then transferred into a 96 deep-well plate and 0.5-fold volume of magnetic beads (Mag-Bind® RxnPure Plus, M1386-01, Omega BIO-TEK) was added ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was sheared to 400 bp using the Covaris LE220 instrument and size selected with SPRI using TotalPure NGS beads (Omega Bio-tek). Library prep included fragment end treatment ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA from specimens identified as Porites lobata (see section above) was extracted using the E.Z.N.A Mollusc RNA kit (Omega Bio-tek, Norcross, GA USA), following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... from approximately 460,000 pooled clones from the ASKA collection (Kitagawa et al. 2005) was isolated using an EZNA Plasmid DNA Mini Kit I (Omega Bio-Tek). Electrocompetent E ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Amplified DNA was run on a 2% agarose gel and were excised and purified using the MicroElute Cycle-Pure Kit (Omega Bio-Tek). Purified DNA was Sanger sequenced by Cornell Institute of Biotechnology ...
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bioRxiv - Evolutionary Biology 2020Quote: Whole genomic DNA was extracted from either the whole specimen or pereopods 6–7 depending on the size of the amphipod using an EZNA Tissue DNA kit (Omega Bio-tek Inc.) following manufacturer’s protocols ...
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bioRxiv - Microbiology 2019Quote: Rumen fluid samples were thawed on ice and microbial DNA was extracted using a commercial DNA Kit (Omega Bio-tek, Norcross, GA, U.S.) according to manufacturer’s instructions ...