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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for rno mir 125b 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... RT-qPCR was carried out using the ViiA7 RT-qPCR system with TaqMan reagents using TaqMan primers/probes (Life Technologies) for EMCV 5′UTR (Table S1).
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bioRxiv - Evolutionary Biology 2020Quote: ... using 48 pairs of in-house DENV serotype-specific primers and the SuperScript III One-Step RT-PCR system with Platinum® Taq High Fidelity polymerase (ThermoFisher Scientific, MA, USA), followed by cDNA purification using Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5’ TCTCGCTGGGGACTCTGGTTGAAAT 3’) primers (Eurofins, Louisville, KY) and SuperScript™ III One-Step RT-PCR kit with Platinum™ Taq DNA Polymerase (Invitrogen, Carlsbad, CA) using the following thermocycler conditions ...
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bioRxiv - Physiology 2021Quote: ... mRNA levels were quantified using gene-specific primer pairs by real-time RT-PCR on an ABI PRISM 7900HT Sequence Detection System (Thermo Fisher Scientific, MA, US) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... quantitative RT-PCR (qRT-PCR) was done using Power SYBR PCR master mix (Thermo Fisher) and in technical triplicates each time.
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bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR (RT-PCR) was conducted using the TaqMan PCR Master Mix (Applied Biosystems), with glyceraldehyde-3-phosphate dehydrogenase (gapdh ...
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bioRxiv - Cancer Biology 2022Quote: ... Quantitative RT-PCR (qRT-PCR) was performed using TaqMan Universal PCR Master Mix (Applied Biosystems) and TaqMan gene expression assays (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized using an oligo(dT) primer and SuperScript III RT (Invitrogen). Template cDNA was diluted such that <30% of reactions were positive in the subsequent PCR ...
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bioRxiv - Genomics 2021Quote: miRNAs were retro-transcribed with gene-specific primers and multiscribe RT (Life Technologies). Their levels of expression were measured with TaqMan probes (miRNA Taqman assays # 000546 for miR-335-5p ...
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bioRxiv - Molecular Biology 2021Quote: ... First strand synthesis was performed with Random Primer and Superscript II RT (Invitrogen) at 25°C for 10 min ...
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bioRxiv - Plant Biology 2024Quote: ... All primers used for the RT-qPCR analyses were synthesized by ThermoFisher (US) or IDT (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... 32 µL of RevertAid RT Reverse Transcription Kit using random hexamer primers (Thermofisher) prepared using the manufacturer’s protocol was aliquoted into a separate 96-well PCR plate and 8 µL of the lysis samples were added and mixed ...
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bioRxiv - Plant Biology 2020Quote: ... The primers used for qRT-PCR were designed with Primer Express v3.0 software (ThermoFisher Scientific). The primers used for qRT-PCR are listed in Table S2.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Primers for PCR were designed using the Primer Express software version 3.0 (Applied Biosystems, USA). Sequences of PCR primer (in 5’→3’ direction ...
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bioRxiv - Microbiology 2021Quote: ... and diagnostic PCRs were set using Taq Phusion DNA polymerase (Invitrogen). The TGME49_233160-HA parasite line was generated as part of an earlier study by tagging of the endogenous locus in the T ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR was set up with Fast SYBR Green mix (Invitrogen) and using the Applied Biosystems™ 7500 Fast Real-Time PCR Instrument ...
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bioRxiv - Microbiology 2021Quote: ... isolated from Aag2 cells was used as a template for RT-PCR performed using SSIII One-Step RT-PCR Kit with Platinum Taq (Invitrogen, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... in 20 μL reactions using AgPath-ID™ One-Step RT-PCR Reagents 10 µl RT-PCR buffer (2X) (Thermo Fisher), 4 μL of RNA ...
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bioRxiv - Immunology 2021Quote: ... The Real-time RT-PCR assays were conducted using TagMan One-Step RT-PCR kit (Thermo Fisher Scientific, Waltham, MA, USA) on Real-Time PCR System (Biorad ...
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bioRxiv - Molecular Biology 2020Quote: ... being 10 μl of 2X RT-PCR Buffer and 0,8 μl of Enzyme Mix of the AgPath-ID™ One-Step RT-PCR Reagents (ThermoFisher™), 0,8 μl of each primer and 0,4 μl of probe for E gene [13] both in a 5 μM concentration ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 μl total volume being 10 μl of 2X RT-PCR Buffer and 0,8 μl of Enzyme Mix of the AgPath-ID™ One-Step RT-PCR Reagents (ThermoFisher™), 1,5 μl of primers + probe ...
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bioRxiv - Molecular Biology 2024Quote: ... RT-PCR was performed with 100 ng of total RNA using SuperScript III One-Step RT-PCR System (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... RNA from blood and tissue samples from NPs were used for RT-PCR amplification using SuperScript™ IV One-Step RT-PCR System kit (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... RT-PCR assays were performed using the SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase (ThermoFisher Scientific) following manufacturer’s conditions ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time Primer sample sets for TaqMan amplifications were obtained from Life Technologies (Darmstadt, Germany). Here ...
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bioRxiv - Microbiology 2021Quote: ... Delta-delta CQ was calculated using the 18S ribosomal RNA primer/probe set (4331182; Thermo Fisher). Virus RNA (vRNA ...
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bioRxiv - Bioengineering 2021Quote: ... We prepared LAMP primer mix and probe set mix stocks in nuclease-free water (Thermo Fisher) and stored at -20 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... 1μL cDNA template with 0.5μL premade primer sets (SOS1: 4331182 Hs00893128_m1, SOS2: 4331182 Hs01127273_m1, GAPDH: 4331182 Hs03929097_g1; Thermo Scientific). This was followed by Amplitaq activation at 50°C for 2 minutes ...
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bioRxiv - Genomics 2022Quote: ... water and primers to set up a standard qPCR run on the QuantStudio instrument (Applied Biosystems). For the transcription factor induction experiments using the transduced cell-lines ...
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bioRxiv - Molecular Biology 2023Quote: ... LOX-4 (Mm 00446385_m1) and 18S ribosomal RNA primer/probe set (Hs 03003631; Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... and PCR amplified with barcoded primers using Phire Plant Direct PCR Mix (ThermoFisher). The PCR amplicons were purified and pooled for NGS ...
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bioRxiv - Immunology 2020Quote: ... and RNA was hybridized first using RT primer (/5BiosG/AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TVN) then reverse-transcribed into cDNA using TSO primer (AAGCAGTGGTATCAAC-GCAGAGTACATrGrGrG) and RT maxima reverse transcriptase (Thermo Scientific). cDNA was amplified using ISPCR primer (AAGCAGTGGTATCAACGCAGAGT ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR program was set on a StepOne™ Real-Time PCR Machine (Applied Biosystems, UK) as follows ...
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bioRxiv - Immunology 2021Quote: Reactions for qRT-PCR were set up using the One-Step qRT-PCR Kit (Thermo Fisher SuperScript III Platinum ...
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bioRxiv - Microbiology 2020Quote: ... PCR reactions were then set up using Power SYBR Green PCR Master Mix (Invitrogen, Cat #: 4367659), 1 μl of cDNA and 0.2 μM primers (Table S5) ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed with AgPath-ID One-Step RT-PCR reagents (Thermo Fisher Scientific), panRusV-2 primers (final concentration ...
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bioRxiv - Genomics 2022Quote: RNA was reverse transcribed for 20 min at 54°C with 1.5 µL RT primer and 10 µl RT mix (0.6 µl Superscript III (Thermo Fisher cat# EP1756B012), 2.17x SuperScript III RT buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Methylated RNA species were recovered from the elution and reverse transcribed (RT) using the RT primer and SuperScript III (Invitrogen 18080044). Excessive RT primer was digested with ExoSAP-IT (Applied Biosystems 78250) ...
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bioRxiv - Cancer Biology 2020Quote: ... Primers for qRT-PCR were purchased from Life Technologies: Arg1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Custom PCR primers (DNA) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was reaction mixture was set up using TaqMan Fast Advanced Master Mix (Thermo Fisher) and Taqman probes for POLDIP2 (Hs00210257_m1 ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative reverse transcription-PCR (qRT-PCR) was performed using the StepOnePlus RT-PCR system (Applied Biosystems) and the 5x HOT FIREPol® EvaGreen® qPCR Supermix kit from Solis BioDyne ...
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bioRxiv - Cancer Biology 2023Quote: ... Real-Time PCR was performed for miR-4521 expression analysis using miRNA Taqman assay (Applied Biosystems, USA) and gene expression analysis using PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... specific mRNA or miR-143/145 was amplified using Power SYBR Green PCR Master Mix (Applied Biosystems) or Taqman Universal PCR Master Mix ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative RT-PCR was carried out using a PCR system (StepOne; Applied Biosystems) and the Luna One-Step RT-qPCR Kit (NEB ...
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bioRxiv - Evolutionary Biology 2021Quote: ... CD33m was amplified by PCR using SuperScript III One-Step RT-PCR (Invitrogen) and Gene-specific primers 5’-TTATATGCTAGCGCCACCATGCCGCTGCTGCTACTGCTGC-3’ ...
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bioRxiv - Developmental Biology 2019Quote: ... RT-qPCR PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems) using primers listed in Table 1 ...
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bioRxiv - Cancer Biology 2019Quote: ... RT–PCR was done using SYBR Green PCR Master Mix (Applied Biosystems, 4309155) on a ViiA 7 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR was performed on StepOne® Realtime PCR system (Applied Biosystems) using KAPA SYBR FAST qPCR Kits (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... RT-PCR was performed on a 7500 Real-Time PCR System (Applied Biosystems) using the iTaq Universal SYBR Green Supermix (BioRad) ...