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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for Zika Virus VLP E prM M Proteins since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Cells were lysed in M-PER Mammalian Protein Extraction Reagent (Thermo Scientific) and analyzed for cGAMP content alongside media using the 2’3’ cGAMP ELISA kit (Cayman Chemical).
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bioRxiv - Cell Biology 2024Quote: ... 20 % FBS and 200 ng/mL Human M-CSF Recombinant Protein (ThermoFisher). Six days after the initial isolation ...
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bioRxiv - Microbiology 2019Quote: ... VLPs were incubated for 2 hours in the DNAse and RNAse cocktails (Invitrogen, Carlsbad, CA) with the addition of 14 µL of 20 mg/ml Proteinase K (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... VLPs were buffer exchanged into Dulbecco’s Phosphate Buffered Saline (DPBS) (Thermo Fisher Scientific #: 14190-144) using a 100-kDa Amicon filter (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... and real-time RT-PCR was performed to detect the presence of influenza A virus (M gene) using AgPath-ID™ One-Step RT-PCR Kit (Thermo Fisher Scientific). Positive influenza samples were then selected for whole genome sequencing using next generation sequencing (NGS ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was reverse-transcribed using Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (RT) according to the manufacturer’s instructions (Invitrogen, Thermo Fisher Scientific, cat# 28025013). For quantitative Real Time Polymerase Chain Reaction (RT-qPCR) ...
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bioRxiv - Molecular Biology 2023Quote: Total proteins were harvested from tissue and cell lines lysates using M-PER protein extraction reagent (Thermo Fisher) respectively supplemented with proteinase and phosphatase inhibitors (Sigma ...
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bioRxiv - Immunology 2023Quote: ... Total protein from B16F10 melanoma was harvested using the M-PER mammalian protein extraction reagent (Thermo Fisher Scientific) with the Complete mini EDTA-free (Roche) ...
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bioRxiv - Cell Biology 2023Quote: Total proteins were harvested from tissue and cell lines lysates using M-PER protein extraction reagent (Thermo Fisher) and supplemented with proteinase and phosphatase inhibitors (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... For data analysis peak areas of extracted ion chromatograms of each isotope of compound-specific fragments [M - e-]+ were determined using the TraceFinder software (Version 4.2, Thermo Fisher Scientific) with a mass accuracy (<5 ppm) ...
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bioRxiv - Immunology 2020Quote: Recombinant soluble ZIKV E protein was biotinylated and coupled to AF488 Neutravidin beads (Life Technologies). Microscale-purified mAbs were tested at a single 1:10 dilution ...
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bioRxiv - Immunology 2020Quote: Recombinant soluble ZIKV E protein was biotinylated and coupled to AF488 Neutravidin beads (Life Technologies). Microscale-purified mAbs were tested at a single 1:10 dilution ...
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bioRxiv - Cancer Biology 2019Quote: Cells were lysed directly in M-PER Mammalian Protein Extraction Reagent (ThermoFisher Scientific), separated on a NuPAGE 4-12% Tris-Glycine Gel (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... 8 M guanidine hydrochloride (GuHCl) (for protein biology) was obtained from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed with M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, 78501) supplemented with protease inhibitor cocktail (Roche ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed in M-PER® mammalian protein extraction reagent (Thermo Scientific) containing protease and phosphatase inhibitors ...
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bioRxiv - Microbiology 2021Quote: Cells were lysed in M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific). Protein lysates were run on 4%–12% Bis-Tris gels (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... CL samples were homogenized in M-PER mammalian Protein Extraction Reagent (Thermo Scientific) using tissue homogenizer (Bioneer ...
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bioRxiv - Biochemistry 2019Quote: ... harvested and lysed using M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, 78501) (with 1X Roche Complete Protease Inhibitor and 100 mM NaCl) ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed in M-PER® Mammalian Protein Extraction Reagent (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Cell lysates were generated with M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... then lysed in M-PER™ Mammalian Protein Extraction Reagent (Thermo Fisher 78501). Samples were transferred to an Eppendorf tube and were heated to 95°C (10 minutes) ...
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bioRxiv - Cancer Biology 2022Quote: ... then lysed with M-PER™ Mammalian Protein Extraction Reagent (Thermo Fisher Scientific) supplemented with cocktail (MedChemExpress ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein lysates were prepared using M-PER mammalian extraction buffer (Pierce, Thermo Scientific) supplemented with 1× protease and phosphatase inhibitor cocktail ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were enriched using magnetic streptavidin beads (Dynabeads M-280, Invitrogen, USA). The beads were washed with a series of SDS buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 × 10-4 M MTG and 5% Protein-Free Hybridoma Media II (Gibco).
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bioRxiv - Physiology 2023Quote: ... and cells were lysed in mammalian protein extraction reagent (M-PER; Thermo Fisher) containing protease inhibitors cocktail (Roche) ...
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bioRxiv - Biochemistry 2021Quote: ... a parallel reaction monitoring regime (PRM) was used to select for a set of previously selected peptides on an Orbitrap Eclipse Tribrid mass spectrometer (ThermoFisher Scientific) operating in positive mode and running Tune version 3.3 ...
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bioRxiv - Biophysics 2021Quote: ... The samples from AP-MS were acquired in Partial Reaction Monitoring mode (PRM)61 on a Q-Exactive Orbitrap (Thermo Fisher) connected to a nanoLC easy 1200 (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... crude extracts from 14-day cultures on YES agar medium of both MB_ ΔpsoA and MB_WT were obtained and assessed by high-resolution parallel reaction monitoring (PRM) LC-MS/MS using a Vanquish uHPLC plumbed directly to a Q Exactive Plus mass spectrometer (Thermo Scientific) outfitted with a 75 µm ID nanospray emitter packed with 15 cm Kinetex C18 resin (1.7 µm particle size ...
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bioRxiv - Systems Biology 2022Quote: ... values representing putatively identified specialized metabolites were targeted for fragmentation (MS/MS) analysis via Parallel Reaction Monitoring (PRM) on a Q Exactive Plus mass spectrometer (Thermo Scientific) outfitted with a nanospray ionization source plumbed directly to a Vanquish UHPLC (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... MS2 measurements were conducted with PRM using a target list of all detected compounds created with Compound Discoverer version 3.1 (Thermo Fisher Scientific). All MS2 spectra were acquired within 20 consecutive injections of the same sample.
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bioRxiv - Cancer Biology 2023Quote: ... Mass spectra were acquired in PRM mode on an Q Exactive HF-X Hybrid Quadrupole-Orbitrap MS system (Thermo Fisher Scientific). The MS1 mass range was 340–1,400 m/z at a resolution of 120,000 ...
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bioRxiv - Biochemistry 2024Quote: The synthesized glycated amines were acquired in parallel reaction monitoring (PRM) using a UHPLC-High-Resolution Accurate mass spectrometer (LC-HRAM, Orbitrap, ThermoFisher Scientific) equipped with a heated electrospray ionization (HESI ...
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bioRxiv - Microbiology 2022Quote: ... PV-3 SC8 VLPs or infectious PV-1 were incubated with 5 µM SYTO9 (Thermo Fisher) and SYPRO red (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... VLPs were expressed with the Bac-to-Bac TOPO Expression system (Invitrogen, Catalog nos. A11101, A11100) according to instructions from the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... Stained VLPs were image with Talos L120 transmission electron microscope (TEM, Thermo Fisher Scientific, former FEI) with 47000x magnification ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were extracted from CEC or FFE cells in M-Per™ Mammalian Protein Extraction Reagent (Thermo Fisher Scientific) using the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: Proteins were prepared from flash frozen cell pellets using M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, Cat # 78503) following vendor’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extraction was performed lysing the cells with M-PER (Mammalian Protein Extraction Reagent, ThermoFisher Scientific, Waltham, MA, USA) lysis buffer plus Halt Protease and Phosphatase Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... BacMam 2.0 virus (Invitrogen, C10593).
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bioRxiv - Biochemistry 2019Quote: ... CO-saturated buffer (950 μM CO) was prepared by sparging 3 mL of anaerobic Buffer E for 15 minutes in a Reacti-Vial (Thermo Fisher Scientific). CO was added to the sample to achieve a final concentration of 425 μM ...
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bioRxiv - Evolutionary Biology 2023Quote: ... into the packaging 293T cells (with molar ratio for Luc-T20: N: M/E: S as 3: 1: 1: 1) with LipofectamineTM 2000 (Thermo Fisher Scientific). At 24 and 48 hours post-transfection ...
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bioRxiv - Microbiology 2019Quote: ... The purified virus was quantified using the bicinchoninic acid (BCA) protein assay kit (ThermoFisher Scientific, Waltham, MA, USA) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: Recombinant soluble ZIKV E protein was biotinylated and coupled to red fluorescent Neutravidin beads (Life Technologies). Antibodies were diluted to 5 μg/mL in RPMI-1640 ...
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bioRxiv - Immunology 2023Quote: ... Anti-flavivirus E protein mAb 4G2 was conjugated to Alexa Fluor 488 5-SDP (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: To assess thermodynamic stability of human and bat (M. myotis and M. capaccinii) purified IgG molecules we used Protein Thermal Shift Dye Kit (Applied Biosystems, Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Loss of virus was monitored by staining with anti-Sendai virus antibody (Invitrogen, cat #14649482).
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bioRxiv - Neuroscience 2022Quote: Parallel reaction montitoring (PRM) analyses were performed as reported earlier (Sathe et al) using an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific) coupled to a nano-UPLC Dionex pump ...
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bioRxiv - Developmental Biology 2023Quote: ... media was changed to Hepatocyte Medium E (William’s E Medium [Gibco] ...