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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Zika Virus VLP E prM M Proteins since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... E and M proteins were resuspended using trypsin (Thermo Fisher Scientific) (1 in 5 dilution in PBS) ...
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bioRxiv - Biochemistry 2022Quote: HIV-1 virus-like particles (VLPs) were produced by Lipofectamine 3000 (Thermo Fisher Scientific) transfection of 293FT cells (purchased from Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... site-directed mutagenesis of prM/E in a pHLsec vector51 was performed using Pfx DNA polymerase (Invitrogen). The pHLsec-prM/E wild-type and point mutants were then further amplified via polymerase chain reaction (PCR) ...
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bioRxiv - Microbiology 2020Quote: Zika virus RNA yields were assessed using real-time PCR on a 7500 Fast Real-Time PCR instrument (Thermo Scientific, Poland). ZIKV cDNA was amplified in a reaction mixture containing 1× TaqMan Universal PCR Master Mix (RT-PCR mix ...
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bioRxiv - Microbiology 2019Quote: ... 8 x 10^5 HEK-293T/17 cells pre-plated in a 6-well plate were co-transfected with a mass ratio of 1:3 replicon:C-prM-E plasmids using Lipofectamine 3000 (Cat# L3000-015; ThermoFisher Scientific). Four hours post-transfection ...
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bioRxiv - Immunology 2021Quote: ... the WNV replicon and C-prM-E plasmids were co-transfected into HEK-293T cells using Lipofectamine 3000TM transfection reagent (Invitrogen). Transfected cells were incubated at 30°C and RVP-containing supernatants were harvested 3-6 days post-transfection and pooled ...
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bioRxiv - Microbiology 2023Quote: ... The following day each well was co-transfected with 1 µg of replicon-encoding plasmid and 3 µg of C-prM-E-encoding plasmid using Lipofectamine 3000 (Cat# L3000-015; ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and 3 µg of a plasmid expressing the WT or mutant C-prM-E structural genes of interest using Lipofectamine 3000 (Cat# L3000-015; ThermoFisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: Chimeric VLPs or monomeric recombinant proteins were coated onto Maxisorp microtiter plates (Nunc) at 1μg/ml in PBS and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: The purified JEV structural proteins (C, M, E, ED III) from the S2 insect expression system (Invitrogen) were quantified by using the bicinchoninic acid (BCA ...
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bioRxiv - Molecular Biology 2020Quote: ... and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen) with 250 μM deoxy nucleoside triphosphate (dNTP ...
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bioRxiv - Neuroscience 2023Quote: ... and Moloney murine leukemia virus (M-MLV) Reverse Transcriptase (Invitrogen). One microgram of total RNA was retrotranscribed and the cDNA was diluted 1:10 to the quantitative PCR (qPCR) ...
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bioRxiv - Microbiology 2023Quote: ... dNTP and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen) were used to synthesize cDNA ...
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bioRxiv - Microbiology 2021Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the SARS-VoV-2 S protein were co-transfected into 293T cells (ATCC) ...
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bioRxiv - Microbiology 2020Quote: ... VLP-WT and VLP-cvD were expressed using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The protein concentration of the purified VLPs was measured using a Pierce™ BCA Protein Assay kit (Thermo Fisher). VLPs were then flash frozen in liquid nitrogen and stored at −80 °C until use ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 6 min and 10 min parallel reaction monitoring (PRM) methods are described (UltiMate 3000 RSLCnano and PRM–QExactive Plus; Thermo Scientific, Waltham, MA, USA). Raw data were processed using TraceFinder 4.1 (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... A polyclonal rabbit anti-prM (1:1000; Invitrogen PA5-34966) and polyclonal rabbit anti-Env (Invitrogen PA5-32246 ...
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bioRxiv - Bioengineering 2023Quote: Concentrated EV or VLP total protein concentrations were determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific, USA) These neutralization reagents were mixed with 4X loading dye ...
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bioRxiv - Microbiology 2021Quote: ... MHV-A59 was quantified by RT-qPCR by amplifying a fraction of its ORF5 gene for the structural protein M.15,16 The TaqMan™ Fast Virus 1-Step Master Mix Kit (Thermo Fisher Scientific Inc., 4444432) was used for RT-qPCR ...
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bioRxiv - Microbiology 2022Quote: ... All PRM data was analyzed using Skyline 74 and FreeStyle (Thermo Scientific) software.
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bioRxiv - Bioengineering 2024Quote: VLP and scFc-VLPs (4µM) were applied onto a glow-discharged carbon-supported copper TEM grid (TEM-CF200CU50, Thermo Fisher Scientific) and incubated for 60 s before gently removing the solution ...
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bioRxiv - Immunology 2023Quote: Proteins in VLP containing samples were separated by SDS-PAGE using 4-12% Bis-Tris Nu-PAGE gels (Invitrogen) and electro-transferred to a PVDF membrane using the Trans-Blot Turbo Transfer Pack (BioRad) ...
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bioRxiv - Bioengineering 2021Quote: ... Dynabeads M-280 Protein G (ThermoFisher, 10003D) were resuspended by vortexing for 30 s ...
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bioRxiv - Systems Biology 2022Quote: ... Dynabeads M-280 Protein G (ThermoFisher, 10003D) were resuspended by vortexing for 30 s ...
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bioRxiv - Systems Biology 2023Quote: ... Dynabeads M-280 Protein G (ThermoFisher, 10003D) were resuspended by vortexing for 30 s ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 E protein was purchased from ThermoFisher Scientific (Catalog# RP-87682) ...
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bioRxiv - Genetics 2021Quote: RNA was reverse transcribed into cDNA using the Moloney Murine Leukemia Virus (M-MLV; Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... DEPC treated water and Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Applied Biosystems, 850 Lincoln Centre Drive ...
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bioRxiv - Biochemistry 2020Quote: ... RNA was reverse transcribed using Moloney Murine Leukemia Virus (M-MuLV) reverse transcriptase (Thermo Fisher Scientific), in the presence of oligo (dT)15 primers ...
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bioRxiv - Systems Biology 2021Quote: ... and analyzed by MS in a PRM mode (Q Exactive™-plus, Thermo Scientific) as previously described51 ...
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bioRxiv - Biochemistry 2020Quote: ... RNA was extracted from the lysed VLPs using TriReagent (Ambion) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... VLPs were resuspended in Life cell imaging solution (Thermo Fisher) and stored at − 80°C ...
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bioRxiv - Microbiology 2020Quote: ... VLPs were incubated with AnnexinV-PE conjugate (AnV-PE) (Invitrogen) in AnV binding buffer for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The purified VLP fraction was treated with DNase I (Ambion) at 37 °C for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... mammalian protein extraction reagent (M-PER, ThermoFisher Scientific), and DNase/RNase-free water (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2020Quote: ... Dynabeads(tm) M-280 Protein G (ThermoFisher 10003D) were resuspended by vortexing for 30 seconds ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was extracted using M-PER (Thermo Scientific) following the manufacturer’s protocol and quantified by Pierce BCA as described above.
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bioRxiv - Biophysics 2020Quote: ... PCoV_GX and RaTG13 pseudoviruses were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective spike protein into 293T cells (ATCC) ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV and MERS-CoV pseudovirus were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective S proteins into 293T cells (ATCC ...
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bioRxiv - Microbiology 2020Quote: ... reverse transcription was performed with random hexamers and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen). Synthesized RNA was used as a standard (BEI) ...
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bioRxiv - Bioengineering 2023Quote: ... reverse transcription was performed with random hexamers and Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Invitrogen). Synthesized RNA was used as a standard (BEI) ...
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bioRxiv - Microbiology 2019Quote: ... the adherens junction protein E-cadherin was stained using an anti-E-cadherin HECD-1 monoclonal antibody (Invitrogen) at 1:100 with subsequent incubation with the secondary DyLight594 conjugated anti-mouse antibody (1:100) ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was incubated with polyclonal rabbit anti-prM (1:1000, Invitrogen, Cat. #PA5-34966) and purified human anti-Env (fusion loop ...
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bioRxiv - Microbiology 2022Quote: ... and the concentration of total virus proteins were determined by a BCA protein assay kit (Thermo Fisher) (31 ...
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bioRxiv - Microbiology 2020Quote: ... Pelleted VLPs were resuspended in 1X NuPAGE LDS sample buffer (ThermoFisher). Cell lysates were collected by washing cells twice with PBS followed by lysis in CytoBuster ...
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bioRxiv - Microbiology 2021Quote: ... Pelleted VLPs were resuspended in 1X NuPAGE LDS sample buffer (ThermoFisher). Cell lysates were collected by washing cells twice with PBS followed by lysis in CytoBuster (Millipore) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Copies of SARS-CoV-2 E gene were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...