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Citations for Thermo Fisher :
651 - 700 of 4415 citations for Yellow Fever virus lysate 17D strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... cDNA was prepared using Moloney Murine Leukemia Virus reverse transcriptase (Thermo Fisher Scientific) with Random Hexamers (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... cells were given virus production media (DMEM supplemented with 20mM HEPES (Gibco; 15630080) and 30% FBS) ...
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bioRxiv - Immunology 2024Quote: ... the media was changed to virus production medium containing Opti-MEM I (Gibco) + 5 % FBS + 1:100 Glutamax + 1:100 MEM-NEAA (Gibco ...
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bioRxiv - Physiology 2022Quote: ... or immunoprecipitated type I and II PKA holoenzymes from iPSC-CMs whole cell lysate or whole heart lysates from Stk25+/+ or Stk25-/- mice following the manufacturer’s protocol (EIAPKA, Invitrogen). Briefly ...
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bioRxiv - Immunology 2019Quote: ... diluted whole cell lysate and phosphoenriched lysates were processed for SDS-PAGE with NuPage LDS Sample buffer (Life Technologies) and 100mM dithriothreitol (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... Both mock transfected lysate and STING-GFP transfected lysate were separately incubated with antibodies against GFP (Life Technologies A11122). All antibodies were incubated overnight at 4°C with gentle rotation ...
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bioRxiv - Molecular Biology 2023Quote: Each lysate was treated with 0.3 U/μL (1.5 µL for 500 µl of lysate) of RNase I (ThermoFisher # AM2294) for 40 min at 20°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Most protein lysates were separated by running 30-100 μg of lysate on 10% Bis-Tris NuPage gels (Invitrogen) and subsequently ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was centrifuged (158,000 g, 4 °C, 1 h) and the cleared lysate incubated with Ni-NTA agarose (Invitrogen) for 1 h at 4°C ...
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Integrated requirement of non-specific and sequence-specific DNA binding in MYC-driven transcriptionbioRxiv - Molecular Biology 2020Quote: ... and purified from the BL21 (DE3) Arabinose-Inducible bacterial strain (Invitrogen) with an adaptation from the same purification protocol (McDuff et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... cerevisiae strain background (S288C, MATa HIS3Δ1 LEU2Δ0 MET15Δ0 URA3Δ0, Thermo Fisher) was used for all experiments in this study ...
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bioRxiv - Biochemistry 2020Quote: ... Ded1-TAP and eIF4A-TAP strains were obtained from Thermo Scientific Open Biosystems (Waltham ...
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bioRxiv - Biochemistry 2020Quote: ... were transformed into Escherichia coli production strain Rosetta (DE3) cells (Invitrogen). Production of the maltogenic α-amylase LaGH13_20 and the oligosaccharide 1,4-α-glucosyltransferase LaGH13_31B was carried out using a 5 L bioreactor (Biostat B ...
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bioRxiv - Microbiology 2021Quote: Mouse liver microsomes (CD-1 male strain) were purchased from GIBCO. Hamster (Syrian female strain ...
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bioRxiv - Microbiology 2021Quote: ... falciparum 3D7and PfKelch13R539T strain was isolated using TRIZOL (Life Technologies, USA). Equal amount of total RNA was used for cDNA synthesis using the cDNA synthesis Kit (Thermo fisher ...
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bioRxiv - Microbiology 2022Quote: ... pneumoniae strain D39 diluted in phosphate-buffered saline (PBS; Thermo Scientific) into the lateral tail vein [15 ...
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bioRxiv - Biochemistry 2022Quote: ... coli strain BL21-star (DE3) (Thermo Fisher Scientific, Waltham, MA, USA), and the transformed cells were cultured in Luria–Bertani medium at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... while knockout strains were taken from the Yeast Knockout Collection (Invitrogen).
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bioRxiv - Microbiology 2021Quote: FAS was purified from PichiaPink™ yeast strain one (Invitrogen, USA), which was grown according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli BL21 (DE3) strain was purchased from Invitrogen (California, USA). Ni2+-NTA His-bind slurry was obtained from Novagen (Darmstadt ...
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bioRxiv - Microbiology 2019Quote: Strains were grown in LB medium (Fisher Scientific, cat # BP1426-2) at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... or TgGPNΔSRD-expressing strains were harvested and lysed in TriReagent (Invitrogen). RNA was extracted according to the manufacturer’s instructions and further cleaned with PureLink Mini RNA and the concentration was calculated on a Nanodrop (Thermo) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The “BL21” strain is the BL21 Star™ (DE3) (Fisher Scientific) strain ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 Star™ (DE3)pLysS strain (#C602003, Thermo Fisher Scientific) was transformed with pDest-His6-MBP-PDE6D and grown at 37 °C in LB medium supplemented with ampicillin at 1:1,000 dilution from 100 mg/ mL stock ...
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bioRxiv - Biochemistry 2023Quote: ... coli strain was BL21(DE3) from Invitrogen (One ShotTM BL21(DE3), Cat ...
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bioRxiv - Microbiology 2023Quote: ... or 6 was then used to transform into stbl3 strain (Invitrogen) for further plasmid amplification.
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bioRxiv - Microbiology 2023Quote: ... Strains were cultured in brain heart infusion (BHI, Fisher Scientific, NH) at 37 °C under different oxygen conditions as specified in the main text ...
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bioRxiv - Microbiology 2024Quote: ... Escherichia coli strains were grown in Luria-Bertani (LB; ThermoFisher Scientific) medium at 37 °C in a MaxQ 6000 incubator (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... gondii strains were cultured in Lab-Tek chamber slides (177437, Nunc). Infected cells incubated or not with the chelator were then chemically fixed for 2h at room temperature using the fixation solution (2.5% glutaraldehyde in 0.1 M Cacodylate buffer ...
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bioRxiv - Immunology 2021Quote: ... Dead cells were removed using Live/Dead Fixable Near-IR or Yellow Dead Cell Stain Kit (Invitrogen, L34976 or L34959, 1:500) in each panel ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were analyzed on the Attune NxT Flow cytometer for relative mCherry expression (excitation/emission 587/610) under the yellow excitation laser within the 610/620 emission filter channel YL2 (Thermo Fisher Scientific). Cell populations were first gated using forward and side scatter channels and then analyzed using side scatter (y-axis ...
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bioRxiv - Biophysics 2020Quote: ... The biotinylated surface was then incubated with FluoSpheres™ NeutrAvidin™-marked microspheres (200nm in diameter, fluorescent in yellow-green, ThermoFisher, F8774) with 1-5uL in 150uL PBS per hydrogel ...
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bioRxiv - Neuroscience 2019Quote: Wild-type C57/BL6J mice of either gender (aged 8-10 weeks) were used as recipients of yellow-green microsphere (polystyrene FluoSpheres™, 505ex/515em nm, Invitrogen, USA) transplantation ...
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bioRxiv - Biophysics 2019Quote: The sample consisting of 3D fluorescence nanospheres was prepared by diluting yellow-green 100 nm fluorescent beads (FluoSpheres™ Carboxylate-Modified Microspheres, 0.1 µm F8803, ThermoFisher Scientific, USA) into water at a concentration of 3 × 109 particles/mL ...
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bioRxiv - Neuroscience 2021Quote: ... The point spread function of the microscope was estimated from the FWHM value of the x-,y- and z- intensity line profiles from 3D images of 200nm yellow-green fluorescent latex beads (FluoSpheres, F8811, Thermo Fisher Scientific): PSF810x/y = 325 ± 27 (SD ...
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bioRxiv - Systems Biology 2023Quote: ... sucrose for 15 minutes and then 30% (w/v in PBS) sucrose for another 15 minutes before resuspension of the pellet in 200 µl yellow Neg-50 cryoembedding medium (Thermo Fisher, 6502Y). Samples were frozen in small cryomolds half-filled with frozen white Neg-50 cryoembedding medium (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... fluorescently labeled food was prepared by mixing 75 μL of yellow-green 2.0 μm polystyrene microspheres (FluoSpheres carboxylate-modified microspheres, Invitrogen, Carlsbad, CA, USA) with 54 mg of Zebrafeed ...
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bioRxiv - Immunology 2023Quote: ... To assess cell viability cells were first stained for 15 min at room temperature with LIVE/DEAD Fixable Yellow Dead Cell Stain Kit (Thermo Fisher Scientific) in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... INS-1 β-cells under respective treatments were stained with 1 µM LysoSensor™ Yellow/Blue DND-160 (Thermofisher scientific, Cat# L7545) for 5 min followed by imaging using confocal microscope (ZEISS ...
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bioRxiv - Immunology 2023Quote: Cells were harvested at timepoints indicated and following incubation with fixable viability dye (Live/Dead Fixable Yellow or Fixable Viability Dye eFluor780) in PBS (1:1000, Thermo Fisher Scientific), were washed and resuspended in fixation buffer (0.5% paraformaldehyde ...
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bioRxiv - Neuroscience 2023Quote: ... (Ross et al., 2010), goat anti-ChAT (1:200, Millipore AB144P, RRID:AB_2079751, rabbit anti-Lucifer Yellow (1:2000, ThermoFisher Scientific A-5750, RRID:AB_2536190), mouse anti-calretinin (1:5000 ...
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bioRxiv - Developmental Biology 2023Quote: ... The oocyte of each COC was injected with about 10 pL of a 100 mM solution of Lucifer Yellow (L453; Thermo Fisher Scientific), as described previously (El-Hayek et al. ...
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bioRxiv - Immunology 2024Quote: ... Dead cells were excluded with fixable viability dyes (eFluor780 or LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit; Thermo Fisher Scientific). Intracellular staining was performed with a fixation/permeabilization kit (eBioscience) ...
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bioRxiv - Cancer Biology 2021Quote: ... 4X SDS-loading buffer was added to protein lysates and 15 μg of lysate were resolved on a Bolt 4-12% gel (NW04120BOX, Invitrogen). Gels were transferred onto an Immobilon-P membrane (IPVH00010 ...
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bioRxiv - Genetics 2020Quote: Protein lysates were obtained from either pestle-homogenized tissues or cell lysates in M-PER lysis buffer (ThermoFisher; Cat# 78501) supplemented with 1x Complete Mini EDTA-Free protease inhibitor cocktail tablets (Roche Applied Sciences ...
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bioRxiv - Neuroscience 2021Quote: ... 20-25 µg of RIPA or SDS lysate or 1 µg of S4 lysate were separated in a 4-20% Tris-glycine gel (Novex, Invitrogen) and transferred to PVDF membranes ...
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bioRxiv - Molecular Biology 2023Quote: Protein (2 µg total lysate, 5 µg soluble lysate) was loaded onto 4-12% Bis-Tris NuPAGE minigels (Invitrogen, NP0322BOX). 10 µL of precision plus protein kaleidoscope prestained standard (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... lysates were mixed with Coomassie G-250 (ThermoFisher) and resolved using NativePAGE 3-12% Bis-Tris gels (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... lysates were first treated with Proteinase K (Ambion) followed by a beads clean-up (2:1 beads to lysate ratio ...
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bioRxiv - Biochemistry 2020Quote: ... Mitochondrial lysates were mixed with magnetic beads (Invitrogen) cross-linked to primary antibodies against NAGS ...