Labshake search
Citations for Thermo Fisher :
601 - 650 of 4415 citations for Yellow Fever virus lysate 17D strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2019Quote: ... Images were corrected for non-uniform illumination using a profile of the beam created by raster-scanning 200 nm yellow-green fluorescent beads (F8811, ThermoFisher Scientific) through the focal volume ...
-
bioRxiv - Bioengineering 2019Quote: Fluorescent beads: In this study we used Yellow/Green fluorescent nanoparticles with a diameter of 100 nm (FluoSpheres, ThermoFisher Scientific, USA).
-
bioRxiv - Microbiology 2021Quote: ... 3 μg of total RNA from the same yellow-necked field mice were treated with the Dynabeads mRNA DIRECT Micro Purification Kit (Invitrogen, USA) following the manufacturer’s instructions.
-
bioRxiv - Immunology 2021Quote: ... Samples were then allowed to form immune complexes with with 1.0 μm yellow-green fluorescent (505/515 nm) FluoSpheres™ NeutrAvidin (Thermo Fisher) beads coated with biotinylated antigen S or HA antigens ...
-
bioRxiv - Immunology 2022Quote: ... Recombinant MARV-Angola GPdTM (IBT Bioservices) was tethered to Fluospheres NutrAvidin-Microspheres yellow-green or red (Thermo Fisher Scientific, Waltham, MA) using the EZ-link Micro Sulfo-NHS-LC-Biotinylation kit (Thermo Fisher Scientific).
-
bioRxiv - Immunology 2022Quote: ... ticks were injected with 2% (vol/vol) 0.2 µL of yellow-green Carboxylated-Modified Microspheres (Thermo Fisher Scientific, Waltham, MA, USA) diluted in anticoagulant buffer and allowed to recover in incubators for 4 h at 22°C and 95% relative humidity (RH) ...
-
bioRxiv - Immunology 2022Quote: Cells were stained for 30 min at 4 ºC with either LIVE/DEAD fixable violet or yellow dead stain Kit (Life Technologies), with empirically titrated concentrations of the following antibodies ...
-
bioRxiv - Microbiology 2023Quote: ... 100 µl of the above cell suspension was mixed with 1×106 uncoated carboxylated yellow-green fluorescent polystyrene microspheres (Molecular Probes) and incubated at room temperature for 30 min with rotary shaking at 150 rpm ...
-
bioRxiv - Neuroscience 2023Quote: ... multiconductor electrodes were coated with a yellow or a red-shifted lipophilic dye (DiI, ThermoFisher C7000, and DiD, ThermoFisher D12730, respectively) to mark recording sites ...
-
bioRxiv - Molecular Biology 2023Quote: ... a workspace was loaded that had the optimised PMT parameters and pre–defined gates for the detection of 200 nm yellow–green (505/515) FluoSphere beads (Invitrogen, F8848). After the fluid stream and lasers were aligned ...
-
bioRxiv - Cell Biology 2023Quote: ... a workspace with pre–defined gates and optimal PMT settings for the detection of 200 nm yellow–green (505/515) FluoSphere beads (Invitrogen, F8848) was loaded ...
-
bioRxiv - Microbiology 2023Quote: Cells were stained for 30 min at 4 °C with either LIVE/DEAD fixable violet or yellow dead stain Kit (Life Technologies), with empirically titrated concentrations of the following antibodies ...
-
bioRxiv - Immunology 2023Quote: ... LIVE/DEAD™ Fixable NearIR Dead Cell Stain Kit or LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit (Thermo Fisher) were used in all experiments to exclude dead cells ...
-
bioRxiv - Cell Biology 2023Quote: ... Immunostained cells were analyzed using an Attune NxT flow cytometer (violet, blue, yellow, and red lasers configuration, Thermo Fisher, Waltham, MA).
-
bioRxiv - Bioengineering 2024Quote: To characterize the resolution of the microendoscope we imaged 1 µm yellow-green (505/515) FluoSpheres (Life Technologies Corporation, Eugene, USA) suspended on poly-D-lysine (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2019Quote: Genomic DNA was extracted from the PhaZ-producing strains (RNA/DNA purification kit, Norgen Biotek for C. testosteroni, and GeneJET Genomic DNA Purification Kit, ThermoFisher Scientific for other strains). Inserts were obtained by amplifications of the mature phaZ genes (without signal peptides ...
-
bioRxiv - Microbiology 2020Quote: ... supernatant was harvested for virus titration and cells were lysed in Trizol (Invitrogen). MHV in the supernatant was quantified by standard plaque assay on L2 cells.
-
bioRxiv - Cell Biology 2020Quote: ... HEK-293T cells used for virus production were cultured in IMDM (Life Technologies) supplemented with 20% IFS ...
-
bioRxiv - Neuroscience 2020Quote: ... Virus-containing supernatant was harvested three days after transfection with Lipofectamine 2000 (Thermofisher). This supernatant was then cleared from cell debris by centrifugation at 3500 rpm for 15 min and filtered through a 0.45 µm filter (Millipore) ...
-
bioRxiv - Neuroscience 2021Quote: ... to wash the virus in Opti-MEM™ Medium (ThermoFisher Scientific, Darmstadt, Germany) and concentrate to a final volume of 150 μl.
-
bioRxiv - Neuroscience 2020Quote: ... They were reprogrammed into iPSCs using the replication incompetent Sendaï virus kit (Invitrogen) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... The virus pellet was then re-suspended in PBS (Thermo Fisher Scientific, 10010015) containing 25 mM HEPES buffer (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... For this the virus was diluted in 3 mL of 199 medium (Gibco). After incubation for 2 h at 37°C the medium was aspirated and 3 mL of fresh DMEM (Gibco ...
-
bioRxiv - Microbiology 2022Quote: ... respectively) and 5 μl of 4X TaqMan Fast Virus 1-step mastermix (ThermoFisher). The qPCR plate was sealed and read in the FAM and HEX channels using a QuantStudio™ 5 Real-Time PCR system (ThermoFisher ...
-
bioRxiv - Microbiology 2021Quote: ... using the Taqman Fast Virus 1-stepMaster Mix enzyme (Thermo Fisher, Waltham, MA). The limit of detection of this assay is estimated to be 200 genome equivalents/ml saliva or swab fluid ...
-
bioRxiv - Neuroscience 2020Quote: ... Cellular reprogramming experiments were conducted using the Sendai virus (CytoTune, Gibco, Life Technologies) carrying the four factors Oct4 ...
-
bioRxiv - Neuroscience 2020Quote: ... Cellular reprogramming experiments were conducted using the Sendai virus (CytoTune, Gibco, Life Technologies) carrying the four factors Oct4 ...
-
bioRxiv - Microbiology 2021Quote: ... or Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a LightCycler 96 or LightCycler 480 instrument (Roche Diagnostics ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 1X TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific; 4444434) for a total reaction volume of 20 μl in 96-well PCR plates (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion) and a mixture of anchored oligo-dT and random decamers (IDT) ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) in a total reaction volume of 10 μl ...
-
Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: ... ten-fold dilutions of virus were prepared in diluent medium (Opti-MEM (Invitrogen) with 2% FBS (Gibco ...
-
bioRxiv - Microbiology 2020Quote: ... The virus was rescued and propagated in T-Rex 293 HEK cells (Invitrogen) which repress antigen expression during virus propagation ...
-
bioRxiv - Immunology 2021Quote: ... human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the SARS-VoV-2 S protein were co-transfected into 293T cells (ATCC) ...
-
bioRxiv - Genomics 2021Quote: ... hiPSCs were generated from primary fibroblasts using Sendai virus reprogramming kit (ThermoFisher Scientific), yielding 2-3 different clonal lines for each individual ...
-
bioRxiv - Cancer Biology 2022Quote: ... packaging cell line for virus production were cultivated in DMEM high glucose (Gibco), supplemented with 10% heat-inactivated fetal bovine serum (Gibco ...
-
bioRxiv - Immunology 2022Quote: ... or mouse anti-Hepatitis C virus Core protein (clone C7-50, Life Technologies) were visualised using IRDye 800CW Donkey anti-Rabbit IgG or Goat anti-Mouse IgG (LiCor) ...
-
bioRxiv - Microbiology 2022Quote: ... or Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a LightCycler 96 or LightCycler 480 instrument (Roche Diagnostics ...
-
bioRxiv - Immunology 2022Quote: ... and Toledo virus was conjugated with DMSO-dissolved AF647–N-hydroxysuccinimide ester (Invitrogen) with constant agitation at room temperature for 1 hour ...
-
bioRxiv - Immunology 2022Quote: ... the master mix used was TaqMan Fast Virus 1-Step Master Mix (ThermoFisher). Manufacturer’s protocol was followed to add the appropriate volumes of master mix ...
-
bioRxiv - Microbiology 2022Quote: Purified virus particles were incubated with different concentrations of BS3 cosslinkers (ThermoFisher Scientific) for 30 min at room temperature ...
-
bioRxiv - Cancer Biology 2023Quote: ... Virus-containing medium was replaced with medium containing puromycin (3.5 μg/ml, Gibco) 24h later ...
-
bioRxiv - Neuroscience 2022Quote: ... PBMCs were expanded and reprogrammed using Sendai virus vector (CytoTune-iPS 2.0, Invitrogen) using a feeder-free system (Sommer et al. ...
-
bioRxiv - Cell Biology 2023Quote: ... Reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase (ThermoFisher Scientific). Real-time PCR was performed using TaqMan assays (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... MCC virus-positive MKL-1 cells were cultured in 1x RPMI (ThermoFisher Scientific) supplemented similarly as stated above ...
-
bioRxiv - Immunology 2023Quote: ... RNA from virus stocks was purified using TRIzol Reagent (Thermo Fisher Scientific, #15596026). Briefly ...
-
bioRxiv - Molecular Biology 2023Quote: ... The virus-containing media were filtered through a 0.45 µm filter (ThermoFisher Scientific) and mixed with fresh media at a 1:1 ratio before adding to pre-seeded target cells ...
-
bioRxiv - Immunology 2024Quote: ... the media was changed to virus production medium containing Opti-MEM I (Gibco) + 5 % FBS + 1:100 Glutamax + 1:100 MEM-NEAA (Gibco ...
-
bioRxiv - Microbiology 2023Quote: ... Nuclease treatment of virus stocks was performed at room temperature by DNase (Ambion), RNase (Qiagen ...
-
bioRxiv - Immunology 2024Quote: ... and the MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies). The relative quantification of the genes of interest was carried out in a 10 μl reaction volume in white ultraAmp 384 well PCR plates (Sorenson ...