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Citations for Thermo Fisher :
351 - 400 of 1692 citations for Yeast Extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... The chromatin extract was treated with DNAse I (Thermo Scientific) and Proteinase K (Thermo Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... supplemented with 50 mg/mL bovine pituitary extract (BPE; Gibco) and 5 ng/mL prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53 ...
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bioRxiv - Cancer Biology 2023Quote: Cell extracts were prepared using RIPA buffer (Thermo Fisher Scientific), or cell culture supernatant and diluted in sample buffer with β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2023Quote: ... containing 50ug/ml of bovine pituitary extract (BPE; Life Technologies) and 5ng/ml EGF (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... RNA extracts were assessed on a Nanodrop 2000c (Thermofisher Scientific) and confirmed to contain >2.5 µg of RNA with a purity of A260/280 = 1.8-2.2 ...
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bioRxiv - Neuroscience 2023Quote: ... 35 μg/mL bovine pituitary extract (Gibco 13028014, Thermo Fisher), 5% FBS (F7524 ...
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bioRxiv - Neuroscience 2023Quote: ... 35 μg/mL bovine pituitary extract (Gibco 13028014, Thermo Fisher), 5% FBS (F7524 ...
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pADP-ribosylation Regulates the Cytoplasmic Localization, Cleavage and Pro-apoptotic Function of HuRbioRxiv - Cell Biology 2023Quote: RNA was extracted from cell extracts using Trizol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA extracts were treated with Turbo DNAse (Invitrogen, AM2238) for gDNA depletion following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins extracts were resolved on Tris-Glycine gels (Invitrogen, #XP04205BOX) by electrophoresis and transferred to a nitrocellulose membrane ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We quantified DNA extracts using Qubit fluorometers (Life Technologies, Inc.). We sheared DNA extracted from muscle or liver to a length of approximately 300-600 bp using a Qsonica Q800R sonicator (Qsonica ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA extracts were prepared using TRIzol™ (Invitrogen, 155960189) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: Whole-cell extracts were prepared using RIPA buffer (Thermo Scientific) containing protease and phosphatase inhibitors (Thermo Scientific) ...
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bioRxiv - Biophysics 2024Quote: ... and extracts were incubated with MyOne Dynabeads Streptavidin C1 (Invitrogen). The beads were then washed with a subsequent sequence of wash buffers ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluate was precipitated in the presence of 100mg yeast tRNA (Life Technologies) and sodium acetate (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: A total of 206 strains from the Yeast Deletion Collection (Invitrogen 95401.H2) and other sources were streaked out on YPD agar plates and then restreaked for single colonies on YPD plates containing 300 μg/ml G418 ...
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bioRxiv - Biochemistry 2019Quote: ... The yeast deletion library in the background of BY4741 was obtained from Invitrogen. In S288C background ...
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bioRxiv - Cell Biology 2019Quote: Total RNA samples were extracted from yeast cells using TRIzol (Life Technologies, 145105) and PureLinkTM RNA Mini Kit (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... Previously constructed NPF CDSs54 in the yeast expression vector pYES-DEST52 (Thermo Fisher) were introduced into the Saccharomyces cerevisiae strain INVSc1 (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... Competent yeast cells were generated using the Sc EasyComp transformation kit (ThermoFisher Scientific) and the transformation was performed according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2020Quote: ... RNA was harvested using the RiboPure™-Yeast RNA Isolation Kit (Ambion, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was extracted using RiboPure Yeast RNA Purification Kit (Thermo Fisher Scientific, #AM1926). The quality of the RNA extracted was analyzed using high sensitivity RNA BioAnalyzer Chip (Agilent) ...
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bioRxiv - Biochemistry 2023Quote: ... The SMM slide was first blocked with 500 nM yeast total tRNA (Invitrogen) in screening buffer (10 mM Tris ...
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bioRxiv - Biophysics 2023Quote: Full-length human HPRT1 was expressed in yeast from the pYES2 vector (Invitrogen). An N-terminal RGS6His-tag was inserted upstream of an SRS linker peptide ...
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bioRxiv - Systems Biology 2023Quote: TF ORFs were cloned into yeast expression vectors using LR Gateway Cloning (ThermoFisher). For each TF-pair ...
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bioRxiv - Molecular Biology 2023Quote: ... 3ʹ-dATP was added to RNAs with Yeast Poly(A) Polymerase (ThermoFisher, 74225Z25KU) in a 40-µl reaction containing 1x Yeast Poly(A ...
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bioRxiv - Biophysics 2023Quote: ... clumps of yeast cells were identified manually using MAPS 3.21 (Thermo Fisher Scientific). Automated preparation ...
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bioRxiv - Plant Biology 2024Quote: Yeast two-hybrid assay was performed using the ProQuest Two-Hybrid System (Invitrogen) by PEG mediated transformation following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Protein extracts were then mixed with Dynabeads MyOne Streptavidin C1 (Invitrogen) from 200 μl bead slurry that were pre-washed with RIPA buffer ...
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bioRxiv - Neuroscience 2021Quote: ... The extracts were dried with a SpeedVac Vacuum Concentrator (ThermoFisher Scientific) and resuspended in 500 µL MeOH ...
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bioRxiv - Molecular Biology 2021Quote: Whole-cell extracts were lysed using Pierce RIPA buffer (Thermo Scientific) or NP-40 buffer supplemented with 1x Halt protease inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Microbiology 2019Quote: ... containing 50 mg.L−1 of bovine pituitary extract (Thermo Fisher Scientific) and 5 μg.L−1 of epidermal growth factor (EGF ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Total DNA was extracted using the Extract All kit (Applied Biosystems) and purified with 95% ethanol and 3 M sodium acetate buffer ...
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bioRxiv - Neuroscience 2021Quote: ... DNA extracts and plasmids were quantified using QuantiT PicoGreen reagent (Invitrogen) and adjusted to 1 ng/μl ...
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bioRxiv - Microbiology 2022Quote: ... Total protein extracts were quantified using the QubitTM protein assay (ThermoFisher) according to manufacturer instructions and expression levels of proteins of interest probed by Western blotting ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein extracts were electrophoresed on 12% acrylamide Tris-Glycine gel (Invitrogen), blotted to nitrocellulose membrane ...
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bioRxiv - Cell Biology 2021Quote: ... Total RNA extracts were prepared with PureLink RNA Mini Kit (Invitrogen), with some modifications of the manufacture’s guidelines ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptide extracts were labelled with TMT-11 reagents (Thermo Fisher Scientific) as described below.
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bioRxiv - Cell Biology 2020Quote: ... The RNA extracts were reverse transcribed (QuantiTech Reverse Transcription Kit, Invitrogen) and used for real-time PCR (QuantiTech SYBR Green RT-PCR ...
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bioRxiv - Synthetic Biology 2020Quote: ... extract was probed with FM 4-64 lipid dye (Life Technologies), a lipophilic styrene dye that has low fluorescence in aqueous solution and becomes brightly fluorescent upon incorporation into membranes ...
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bioRxiv - Molecular Biology 2020Quote: ... extract was incubated with 15 µg of RNAse A (ThermoFisher Scientific). Beads were then washed three times with ice-cold HNTG before being resuspended in 2x Laemmli buffer ...
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bioRxiv - Bioengineering 2021Quote: ... The extract was digested using double-stranded DNase (Thermo Fisher Scientific). The digested product was purified by using the ssDNA/RNA Clean & Concentrator Kit (Zymo Research) ...
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bioRxiv - Genetics 2021Quote: ... and bovine pituitary extract (Cat # 12752-010, Thermo Fisher Scientific, USA). MCF7 cells were cultured in DMEM (Cat # 10013CV ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins extracts were resolved on 4-12% Bis-Tris NuPAGE (Invitrogen). Primary antibodies used were rabbit anti-GFP 1:2000 (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: Protein extracts were prepared in RIPA lysis buffer (Thermo Fisher Scientific) from snap-frozen heart tissues ...
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bioRxiv - Cancer Biology 2021Quote: ... In two samples RNA was extract using Trizol reagent (Invitrogen #155906026).
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bioRxiv - Cancer Biology 2022Quote: ... Protein extracts were quantified with Pierce BCA protein Assay (Thermo Scientific), mixed with loading buffer (350 mM Tris-HCl pH 6.8 ...
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bioRxiv - Plant Biology 2023Quote: ... All RNA extracts were precipitated with glycogen RNA-grade (Thermo Scientific), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Extracts were quantified using Pierce BCA assay kit (ThermoFisher Cat. 23225). 5 mM DTT was added to extracts and incubated at room temperature for one hr to reduce proteins ...
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bioRxiv - Immunology 2023Quote: ... Cleared protein extracts were denatured with NuPAGETM LDS loading buffer (Invitrogen) added with 50 mM DTT for 10 min at 75 C and loaded onto precast NuPAGETM 4-12% bis-tris protein gels (Invitrogen) ...