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Citations for Thermo Fisher :
601 - 650 of 1692 citations for Yeast Extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: The hpt1Δ (MatA, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0, hpt1::KanMX) Saccharomyces cerevisiae yeast strain was from Invitrogen. The cells were cultured in synthetic complete (SC ...
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bioRxiv - Biochemistry 2023Quote: Ribosomal RNA was depleted using the Ribo Minus Yeast module (Thermo Fisher Scientific Cat# 45-7013) and libraries were constructed using the Ultra Directional RNA Library Prep Kit (NEBNext Cat# E74205 ...
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bioRxiv - Molecular Biology 2024Quote: ... Coverslips were incubated with FISH probe incubation buffer (2x SSC, 40ng/μL yeast tRNA (Life Technologies), 40ng/μL salmon sperm DNA (Life Technologies) ...
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bioRxiv - Systems Biology 2020Quote: ... longifolia extract was injected into a Dionex Ultimate 3000 UPLC-system (Thermo Scientific, USA) coupled to a micrOTOF-Q hybrid QTOF mass spectrometer (Bruker Daltonics ...
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bioRxiv - Cell Biology 2020Quote: ... 30 μg protein extracts were resolved using 4–12% SDS-PAGE gels (Life Technologies) and transferred to nitrocellulose membranes using iBlot (Life Technologies) ...
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bioRxiv - Microbiology 2019Quote: ... We assayed each feces extract with the Qubit™ dsDNA HS assay (Invitrogen™) to quantify dsDNA concentration ...
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bioRxiv - Microbiology 2021Quote: ... Crude extracts in Bugbuster reagent were purified using HisPur Ni-NTA resin (Thermo Scientific) and run through a Poly-Prep Chromatography Column (BioRad ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein extracts (100 µg of protein) were incubated with streptavidin agarose beads (Thermo Scientific) for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleared extracts were incubated with 200 ul of Dynabeads MyOne Streptavidin C1 (Thermo Fisher) and incubated overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... The extract purity was evaluated with the NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA). DNA was resuspended in 30 μL of TE buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... Proteins extracts were then processed for Western blotting using pre-cast gels (Life Technologies) and transferred on nitrocellulose by semi-dry transfer (Bio-Rad).
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bioRxiv - Systems Biology 2019Quote: Total protein extracts were separated on 4–12% Bis-Tris NuPAGE® gels (Invitrogen) and blotted onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2020Quote: ... DNA extracts were quantified using a Qubit fluorimeter (Thermo Fisher Scientific, Waltham, MA, USA) and stored at −80°C until processing and analysis.
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bioRxiv - Plant Biology 2020Quote: ... DNA extracts were quantified using the PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Several 2 μL droplets of extract were pipetted under heavy mineral oil (ThermoFisher Scientific) in a petri dish ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein extracts were stacked on NuPAGE 4-12% Bis-Tris acrylamide gels (Life Technologies) and cut from the gel ...
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Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumorsbioRxiv - Cancer Biology 2020Quote: ... Dried metabolite extracts were dissolved in 16 μL methoxamine (MOX) reagent (ThermoFisher TS-45950) and incubated at 37° C for 90 minutes followed by addition of 20μL N–methyl–N–(tert–butyldimethylsilyl)trifluoroacetamide + 1% tert–Butyldimethylchlorosilane (Sigma 375934 ...
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bioRxiv - Cancer Biology 2020Quote: ... Dried free metabolite extracts were dissolved in 16µL methoxamine (MOX) reagent (ThermoFisher TS-45950) and incubated at 37°C for 90 minutes followed by addition of 20µL N–methyl–N–(tert–butyldimethylsilyl)trifluoroacetamide + 1% tert– Butyldimethylchlorosilane (Sigma 375934 ...
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bioRxiv - Cell Biology 2021Quote: ... cellular extracts were obtained using Pierce RIPA Buffer (Thermo Fisher Scientific, Waltham, MA, USA) and then sonicated for 3×30 sec on ice at 50% amplitude using the Vibra-Cell VC130 Ultrasonic Processor (Sonics) ...
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bioRxiv - Microbiology 2020Quote: ... RNA extracts were quantified using the QuBit RNA HS Assay Kit (Thermo Fisher Scientific). DNAse treatment ...
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bioRxiv - Microbiology 2020Quote: Extracts were analyzed for phenanthrene content with a Trace GC Ultra system (Thermo Scientific) equipped with a 30 x 0.25 mm (0.25 µm thickness ...
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bioRxiv - Genetics 2020Quote: ... Protein contents of nuclear extracts were quantified using the Qubit Protein assay kit (Invitrogen). Then ...
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bioRxiv - Microbiology 2021Quote: ... First strand cDNA was then created from the RNA extracts using Superscript III (Invitrogen) and 32 µM random pentadecemer primers [45] following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Total RNA from cell extracts was isolated using Trizol (Life Technologies, Carlsbad, CA, USA) as described by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... the remaining extract was mixed with 50 μL of Protein G Dynabeads (ThermoFisher, 10004D) coupled with anti-GFP antibodies (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared nuclear extracts were then subjected to immunoprecipitation using streptavidin magnetic beads (Invitrogen) and IP buffer (20mM HEPES pH 8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... FUS protein extracts were ran on 4-12% Bis-Tris protein gels (NuPAGE, ThermoFisher) with 1X MOPS (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... Ganglioside extracts were analyzed by direct infusion on a QExactive mass spectrometer (Thermo Scientific) equipped with a TriVersa NanoMate ion source (Advion Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: ... 1% Chicken Embryo Extract (CEE, #CE-650-F, Seralab) in DMEM + Pyruvate (#41966, GIBCO) and for FAPs ...
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bioRxiv - Biochemistry 2021Quote: ... The protein concentrations of the extracts were quantified by BCA assay (ThermoFisher Scientific, 23225). For “in-solution” ...
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RNA-seq sample preparation kits strongly affect transcriptome profiles of a gas-fermenting bacteriumbioRxiv - Systems Biology 2022Quote: ... RNA extracts were depleted of DNA using off-column TURBO™ DNase (AM2239; Invitrogen) followed by purification using the RNA Clean & Concentrator™ kit (R1018 ...
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bioRxiv - Immunology 2020Quote: ... and 50 μg/mL Bovine Pituitary Extract and 1% Pen/Strep (ThermoFisher, Mississauga, Canada) at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... HBECs were lysed in Mammalian Protein Extract Reagent (M-PER; Thermo Scientific, Rockford, 78501) with sample buffer solution (Wako ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentrations of the extracts were determined by BCA protein assay (ThermoFisher, Cat. # 23228). Equivalent amounts of protein per sample were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore) ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein quantities in cell extracts were measured with a BCA kit (ThermoFisher Scientific). The proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Systems Biology 2020Quote: Food extracts were analyzed using a UltiMate 3000 UHPLC system (Thermo Scientific, Waltham, Ma) equipped with a reverse phase C18 column ...
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bioRxiv - Genetics 2020Quote: ... Protein extracts were suspended in 50 μL of NuPAGE LDS Sample buffer (ThermoFisher Scientific). Samples (5 μL each ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations in exosome extracts were determined using the BCA protein assay (Thermo Fisher). 20 μg of total lysates were diluted 1:1 in RIPA SDS-PAGE sample buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The RNA was isolated from the resulting extract using Trizol (Life Technologies, Carlsbad, CA) and the RNeasy Plus Micro Kit (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... whole cell extract (40 μg) was mixed with LDS–PAGE sample buffer (Thermo Fisher) containing 5% (v/v ...
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bioRxiv - Cell Biology 2021Quote: Tissue extracts and whole-cell lysates were prepared in radioimmunoprecipitation assay buffer (Thermo Scientific) supplemented with 1 mM PMSF ...
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bioRxiv - Genomics 2021Quote: ... 15 μg of protein extracts were mixed with NuPAGE LDS Sample Buffer (Invitrogen, NP0007), supplemented with 50 mM DTT (Carl Roth ...
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bioRxiv - Immunology 2020Quote: Whole cell extracts were prepared by cell lysis in RIPA buffer (Thermo Fisher Scientific), as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Extracts were quantified using a Quant-iT RiboGreen RNA assay kit (Thermo Fisher Scientific). One whole and one-half filter from each sampling horizon from 2017 were extracted in this manner and the extracts pooled for downstream analysis ...
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bioRxiv - Microbiology 2020Quote: ... Extracts were quantified using a Quant-iT PicoGreen dsDNA assay kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein extracts were directly resolved on Tris-acetate 3-8% polyacrylamide NuPAGE gels (Invitrogen). Proteins were transferred to a nitrocellulose Hybond-C membrane (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... or with RNase A (0.1 mg per every 8 μL of PEARL extract, ThermoFisher) in a final volume of 10 μL for 30 minutes at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... RNA extracts were cleaned with the MEGAclear kit (Thermo Fisher Scientific, Waltham, MA, USA); quality of the RNA was verified by agarose gel electrophoresis and bioanalyzer (2100 Bioanalyzer ...
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bioRxiv - Immunology 2020Quote: ... Nuclear extracts were incubated with GSK675 (biotinylated GSK761) prebound to streptavidin Dynabeads (Thermo Fisher). Dynabeads were incubated with 2X SDS reducing buffer (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Cytoplasmic and nuclear extracts were prepared using Nuclear and Cytoplasmic Extraction reagents (ThermoFisher Scientific). SDS-PAGE electrophoresis ...