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Citations for Thermo Fisher :
651 - 700 of 1692 citations for Yeast Extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... DG extracts were analyzed on a Trace 1310 GC (Thermo Fisher, Waltham, MA USA) equipped with a flame-ionization detector (FID ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein extract concentrations were determined using a Pierce BCA Protein Assay Kit (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Mitochondrial extracts were prepared using Mitochondria Isolation Kit for Cultured Cells (Thermo Scientific, MA) with some modifications ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was purified from cellular extracts using HisPur Ni–NTA resin (Thermo Scientific). The purified protein was labeled with SNAP-Surface Alexa Fluor647 dye (New England Biolabs) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with final concentration of 25 mg/l Bovine Pituitary Extract (Gibco, 13028-014), 294.4 ng/l human recombinant EGF (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: ... The extracts were quantified using the Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA). To check for molecular integrity ...
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bioRxiv - Biochemistry 2022Quote: ... The crude extract was loaded on to an Ultimate 3000 HPLC (Thermofisher, Waltham, MA) coupled with automated fraction collector in batches through a continuous gradient on an Agilent ZORBAX SB-C18 column (Agilent ...
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bioRxiv - Plant Biology 2023Quote: ... oak gall extracts were first dried in a SpeedVac (SPD111V, Thermo Scientific, Waltham, MA), then resuspended in 100% MeOH containing an internal standard mix of isotopically labeled compounds (∼15 µM average of 5-50 µM of 13C,15N Cell Free Amino Acid Mixture ...
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bioRxiv - Biophysics 2023Quote: Lipid extracts were analyzed by direct infusion on a QExactive mass spectrometer (Thermo Scientific) equipped with a TriVersa NanoMate ion source (Advion Biosciences) ...
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bioRxiv - Developmental Biology 2023Quote: ... 20 µl extract each were loaded on two 4-12% gradient polyacrylamide gels (Invitrogen) run in parallel ...
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bioRxiv - Plant Biology 2023Quote: ... RNA extracts were stabilized by adding the RNAseOUT recombinant ribonuclease inhibitor (Thermo Fisher Scientific), before freezing at -80°C until ready for further analysis.
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bioRxiv - Synthetic Biology 2023Quote: The mRNA was purified from total RNA extracts using Dynabeads Oligo (dT)25 (Invitrogen) and eluted from the beads ...
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bioRxiv - Molecular Biology 2023Quote: The crude extracts were separated on 4-12% bis-tris gels (Invitrogen; cat# NP0326BOX) in MES buffer (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... RNA extracts were stabilized by adding the RNAseOUT recombinant ribonuclease inhibitor (Thermo Fisher Scientific), before freezing at −80°C until further analysis.
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bioRxiv - Microbiology 2023Quote: ... Resulting DNA extracts were quantified using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Biotinylated proteins were isolated from the extract using MyOne Streptavidin C1 Dynabeads (Thermo Scientific). To this end ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 1 μL extract was injected onto an EASY-nLC 1000 UHPLC (Thermo Fisher Scientific) and separated on a 15 cm ReproSil-Pur C18-AQ nano LC column (0.1 mm i.d. ...
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bioRxiv - Genomics 2023Quote: ... The concentration of DNA extracts and libraries was measured by Qubit (Thermofisher, Waltham, MA). The fragment length of DNA extracts and libraries was checked by Bioanalyzer (Agilent Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with Bovine Pituitary Extract 50 mg.L-1 (Ref 13028-014, GIBCO, Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: The extracts (1 mg protein) were pre-cleared with control 4%-Agarose resin (ThermoFisher) for 1h at 4 °C with constant rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... Whole-cell extracts were separated using a 4-20% Tris-Glycine polyacrylamide gel (Invitrogen) and transferred to a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracts were quantified using a Pierce BCA Protein Assay Kit (Thermo Scientific #23227). A total of 30-50 μg of proteins were separated via SDS-PAGE using Novex™ WedgeWell™ 4-20% ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracts were quantified using a Pierce BCA Protein Assay Kit (Thermo Scientific #23227). A total of 30-50 μg of proteins were separated via SDS-PAGE using Novex™ WedgeWell™ 4-20% ...
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bioRxiv - Cancer Biology 2023Quote: ... dried metabolite extracts were dissolved in 16 mL methoxamine (MOX) reagent (ThermoFisher TS-45950) and incubated at 37°C for 90 min ...
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bioRxiv - Biochemistry 2023Quote: Protein extracts from cells and tissues were obtained with RIPA Buffer (Thermo Scientific, # 89900). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Whole cell extracts were isolated using the M-PER kit (Thermo Fisher Scientific, USA) as per manufacturer’s instructions ...
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Suppression of the Integrated Stress Response in Islet β Cells Decreases Risk of Autoimmune DiabetesbioRxiv - Cell Biology 2023Quote: ... whole-cell extracts of cells were prepared in a lysis and extraction buffer (ThermoFisher) supplemented with HALT protease inhibitor cocktail (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... The DNA concentration of all extracts was measured on a Nanodrop device (Thermo Scientific). When the concentration was below 2 ng/μl ...
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bioRxiv - Neuroscience 2024Quote: ... whole cell extracts were collected by using M-PER lysis buffer (Thermo Fisher Scientific) supplemented with protease inhibitor and phosphatase inhibitor cocktail (Merck Millipore ...
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bioRxiv - Bioengineering 2024Quote: ... Cleared extracts were used for hydrophilic interaction LC/MS analysis (UltiMate 3000 RSLC, (ThermoFisher), with a 6 150 x 4.6 mm ZIC-pHILIC column running at 300 µl/min-1 and Orbitrap Exactive) ...
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bioRxiv - Cancer Biology 2023Quote: Concentration of nuclear protein extracts was determined using the Bradford assay (ThermoFisher, cat #23238). All MS-IP experiments were done in triplicate ...
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bioRxiv - Cell Biology 2024Quote: ... DNA extracts were quantified using the Qubit dsDNA Broad Range assay kit (ThermoFisher Scientific) according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with bovine pituitary extract as per manufacturer’s instructions (Thermo Fisher Scientific, Cat # 13028014), human growth hormone (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We verified DNA extract concentration using a Qubit 3.0 Fluorometer (Invitrogen, Waltham, MA, U.S.A.). We input up to 50 ng of DNA ...
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bioRxiv - Cell Biology 2024Quote: ... Cell protein extracts were resolved by Bis-Tris polyacrylamide gel electrophoresis gels (Life Technologies) and run in 1 X MOPS buffer (Life Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... Whole cell extracts were resolved by 8-16% Tris-glycine polyacrylamide gel (Invitrogen XP08165BOX), transferred to PVDF membranes with iBlotTM 2 transfer device (Thermo Fisher IB21001 ...
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bioRxiv - Genetics 2023Quote: ... Protein extract was pre-cleared by incubation with 10 μl Protein A Dynabeads (Invitrogen) at 4°C for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... The GFP-tagged and the knockout strains come from the commercial Yeast GFP clone collection (ThermoFisher Scientific). All cultures were grown in YPD medium (1% Yeast Extract ...
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bioRxiv - Developmental Biology 2022Quote: Yeast two-hybrid (Y2H) interactions were characterized by employing the ProQuest Two-Hybrid System (Invitrogen Life Technologies) as reported in Cruz-Ramirez et al. ...
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bioRxiv - Developmental Biology 2022Quote: Yeast two-hybrid (Y2H) interactions were characterized by employing the ProQuest Two-Hybrid System (Invitrogen Life Technologies) as reported in Cruz-Ramirez et al. ...
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bioRxiv - Cell Biology 2019Quote: ... Microarray analysis was conducted with the GeneChip® Yeast Genome 2.0 Array (Affymetrix, Santa Clara, California, USA). Briefly ...
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bioRxiv - Biophysics 2019Quote: ... the yeast codon-optimized gene of the crGE-NLS sequence was amplified from pYES2 vector (GeneArt, Invitrogen) together with pTEF1 and CYC1T by PCR with the forward primer F1_SM and reverse primer R1_SM ...
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bioRxiv - Plant Biology 2020Quote: ... At1g08710 was further subcloned in yeast two hybrid vector pDEST-GBKT7 using Gateway cloning technology (Invitrogen, CA). Cloning was confirmed by colony PCR and sequencing.
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bioRxiv - Plant Biology 2021Quote: ... Plasmids used for yeast two-hybrid or Co-IP were generated by gateway LR cloning (Life Technologies) using the ENTRY vectors described above and appropriate destination vectors listed in Suppl ...
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bioRxiv - Systems Biology 2020Quote: ... The hybridisation was performed in 50μL of Stellaris hybridisation buffer complemented with 250μg.mL-1 Yeast tRNA (Ambion) and 5mM Ribonucleoside Vanadyl Complex (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... The sequence-verified open reading frames were subcloned into a pYES-DEST52 yeast expression vector (ThermoFisher Scientific) by the LR reaction ...
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bioRxiv - Cell Biology 2021Quote: ... or Quasar 570 probes targeting mad1 were mixed with 2 μL each of yeast tRNA (Life Technologies) and Salmon sperm DNA (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Mating-type switch was confirmed by PCR on ultrapure gDNA (Yeast DNA Extraction Kit 78870, ThermoFisher Scientific) with oligos PR_26_D7+PR_26_D8 ...
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bioRxiv - Neuroscience 2020Quote: ... The acetylated sections were washed and incubated in hybridization buffer (50% formamide, 0.25 mg/mL yeast RNA, 0.5 mg/mL herring sperm DNA, 5x Denhard’s, 5x SSC, Invitrogen) at room temperature for 1 hr ...
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bioRxiv - Immunology 2020Quote: The H99S strain [21] of Cn serotype A was grown in yeast peptone dextrose (YPD; ThermoFisher Scientific) broth ...