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Citations for Thermo Fisher :
701 - 750 of 1252 citations for Vertical Electrophoresis Comb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated by electrophoresis in NuPage 4-12% Bis-tris gels run with 1X MOPS buffer (Invitrogen NP0323) and transferred to an iBlot nitrocellulose membrane (Invitrogen IB301002) ...
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bioRxiv - Immunology 2024Quote: ... and uterine tissue lysates (75 μg per well) were subjected to electrophoresis in sulfate-polyacrylamide gels (Cat# NP0336BOX; Invitrogen). The proteins were transferred onto nitrocellulose membranes (Cat# 1620145 ...
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bioRxiv - Plant Biology 2020Quote: ... We controlled the RNA integrity through agarose gel electrophoresis (1.5 %) and RNA concentration and purity were measured with a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Synthetic Biology 2021Quote: ... The treated samples were subjected to SDS-polyacrylamide gel electrophoresis on Bolt™ 12% Bis-Tris Plus Protein Gels (Invitrogen). The separated protein samples were then transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Biophysics 2021Quote: ... The products were then assessed by capillary electrophoresis on an ABI3130 System and results were analyzed using Sequencing Analysis software v5.4 (Applied Biosystems).
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bioRxiv - Cancer Biology 2021Quote: ... equal protein amounts were loaded and then separated by electrophoresis through NuPAGE Novex Bis-Tris 4–12% gradient gels (Invitrogen). Proteins were transferred onto Nitrocellulose membranes using the iBlot system and protocol from InVitrogen (Carlsbad ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were resolved by standard SDS polyacrylamide gel electrophoresis using pre-stained PAGE RULER plus (Thermo Fisher Scientific, cat# 26619) molecular weight markers ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplified fragments were resolved in agarose 0.8% (w/v) gel electrophoresis together with 1 Kb Plus Ladder (Invitrogen, 10787026). While vsp1 ...
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bioRxiv - Microbiology 2019Quote: ... Surveyor assay was performed using Sarm1AG3S PCR conditions and Surveyor Mutation Detection Kit (IDT) followed by electrophoresis on Novex 20% TBE gels (Invitrogen). Off-target CRISPR cleavage was accessed by PCR amplification using the primers listed in table I and cycling conditions 95° 30 sec ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA integrity was determined by agarose electrophoresis and concentration measured using Qubit RNA Assay Kit in a Qubit 2.0 Fluorometer (Life Technologies, Invitrogen). cDNA libraries were synthetized from 1 µg of total RNA and sequenced using a HiSeq-3000 platform (Illumina ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and the resulting 15 uL mixture was separated by gel electrophoresis with a 15% polyacrylamide TBE-urea gel (Invitrogen EC68852BOX) at 200 V for 30 minutes ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were visualised under UV light after electrophoresis in 1% Agarose gels containing SYBR Safe DNA gel stain (Invitrogen). Successful amplifications were purified using the GeneJET PCR Purification Kit (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... of cell extracts was separated by NuPAGE gel electrophoresis (4-12% Midi 1.0 mm Bis-Tris precast gels, Life technologies) and electrotransferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2021Quote: ... and 100 ml methanol in 1 liter of ddH2O) in an Xcell surelock electrophoresis cell (Thermo Fisher, Waltham, MA, USA) under 30 volts for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were boiled at 95 °C for 5 min and separated by SDS–PAGE electrophoresis using 8-16% Tris-Glycine gels (Invitrogen) at 225 V for 30 min ...
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bioRxiv - Genomics 2020Quote: ... PCR products were resolved via capillary electrophoresis using an AB Prism® 3730 Genetic Analyzer (Applied Biosystems, Foster City, CA) and amplicon length evaluated using Marker 1.85 (SoftGenetics ...
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bioRxiv - Cancer Biology 2019Quote: ... The proteins were resolved using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (10%) and then transferred to nitrocellulose membranes (Thermo Fisher). The membranes were first blocked using 5% non-fat milk ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA quality and concentration were determined by denaturing agarose gel electrophoresis and Qubit RNA BR Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2019Quote: ... Samples were boiled and reduced in a mixture containing NuPAGE® Sample Reducing Agent (10X) and 4X Protein Loading Buffer before being resolved by SDS gel electrophoresis on 4-20% Tris/glycine gels (Invitrogen). Proteins were transferred onto a nitrocellulose membrane utilizing a Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... were separated by electrophoresis through 10% (for nuclease knockdown and overexpression experiments) or 15% (for dual reporter experiments) TBE-urea gels (Invitrogen). Following electrophoresis ...
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bioRxiv - Neuroscience 2019Quote: ... The samples were heated to 95°C for 5 minutes prior to electrophoresis through a 12% Bis-Tris precast gel (Invitrogen), followed by transfer to a nitrocellulose membrane by wet blotting ...
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bioRxiv - Genetics 2019Quote: ... The amplified fragments were analyzed by electrophoresis in 2% agarose gels with SYBR Safe DNA gel stain (Invitrogen, Carlsbad, CA), and was observed in an Enduro™ GDS Touch transilluminator (Labnet International Inc ...
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bioRxiv - Cell Biology 2019Quote: ... for 10 minutes and separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) on NuPAGE 4-12% Bis-Tris Gel (ThermoScientific) in 1x Bolt MES SDS Running Buffer (Invitrogen) for 60 minutes at 130V.
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bioRxiv - Cancer Biology 2020Quote: ... Thirty μg of protein in each sample was resolved by electrophoresis using 4-12% bis-tris gels (Thermo Fisher Scientific) and transferred to nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... Reaction products were separated by gel electrophoresis on 4-12% Bis-Tris gradient gels using MES SDS running buffer (Invitrogen) and stained with Flamingo fluorescent protein stain (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: Gel electrophoresis was performed using 4-12% NuPAGE Bis-Tris gels according to manufacturer’s protocols (NuPAGE system, Thermo Fisher Scientific). Protein gel bands were excised ...
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bioRxiv - Genomics 2019Quote: ... Digestion and ligation efficiencies were assessed by gel electrophoresis on a 0.9% agarose gel and also by quantitative PCR (SYBR green, Thermo Fisher).
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bioRxiv - Neuroscience 2020Quote: ... Libraries were visualized by electrophoresis on a 1% agarose and 1X TAE gel containing 0.3 μg/mL ethidium bromide (Invitrogen, 15585011). Gel extraction was performed to select for products between 200 – 1000 bp using the Nucleospin Gel and PCR Clean-up kit (Macherey-Nagel ...
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bioRxiv - Biochemistry 2020Quote: Purified rFIX samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using a 4-12% Bis-Tris Bolt acrylamide gel (Invitrogen) in 1x MES running buffer (Invitrogen) ...
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bioRxiv - Bioengineering 2021Quote: ... The PCR products were subjected to agarose gel electrophoresis and purified using the GeneJET Gel Extraction Kit (Thermo Scientific™). Then the ORFs were cloned into the pDONR221 vector in presence of BP clonase (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: The purified DNA fragments separated by electrophoresis in 1% agarose gel and transferred by the alkaline capillarity method to the Bio-dyne nylon membrane (Invitrogen) in a denaturing solution (NaOH 0.4 M) ...
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bioRxiv - Neuroscience 2021Quote: Protein samples in 1X SDS loading buffer/100 mM dithiothreitol (DTT) were resolved by gel electrophoresis on acrylamide NuPAGE Novex 4-12% Bis-Tris Protein Gels (Invitrogen) with NuPAGE MES SDS Running Buffer (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: Proteins were separated by electrophoresis using a NuPAGE 4-12% Bis-Tris gradient gel run in MES buffer (Life Technologies) and transferred to a Hybond-P PVDF membrane (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... Twenty μL of clarified lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 4-12% or 10% polyacrylamide Bis-Tris gels (Invitrogen) and transferred onto Immobilon FL PVDF or P PVDF membranes (Millipore ...
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bioRxiv - Plant Biology 2021Quote: ... DNA quality was checked by agarose gel electrophoresis and quantified with a Qubit® fluorometer (Thermo Fisher Scientific, Waltham, MA). Library construction and sequencing was based on the 96-plex genotyping-by-sequencing (GBS ...
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bioRxiv - Biochemistry 2021Quote: ... gondii parasites were separated by polyacrylamide gel electrophoresis (PAGE) in precast NuPAGE (4-12% or 12%) or NativePAGE (4-16%) gels (ThermoFisher) according to the manufacturer’s instructions with minor modifications (detailed in the SI) ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were size-selected (50-400 bp) using agarose gel electrophoresis and quantified fluorometrically using a Qubit 3.0 Fluorometer (Life technologies), and a total of 10 ng of digested DNA (200 pg l-1 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The purified RNA samples were prepared for gel electrophoresis by adding 1 μl loading dye (gel loading buffer II, Invitrogen) followed by incubation at 95°C for 5 minutes ...
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bioRxiv - Microbiology 2021Quote: ... the extracted DNA samples were checked for quality and quantity by a 1% agarose gel (Midori Green-stained) electrophoresis and a Qubit dsDNA HS Assay Kit (Invitrogen). Prior to library preparation ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were separated by electrophoresis on a 1.5% agarose gel and stained with SYBR® safe (Thermo Fisher Scientific) before being visualized under an ultraviolet transilluminator ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA fragments resulting from nuclease activity during the Surveyor assay were separated by electrophoresis in 10% TBE-polyacrylamide gels (ThermoFisher). A quantitative measurement of optical density through Image J was performed to quantify efficiency of CRISPR/Cas9 editing ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were analyzed by 1% agarose gel electrophoresis and visualized under UV illumination after SYBR-Safe staining (Invitrogen, S33102). For Sanger sequencing ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR products were resolved by agarose gel electrophoresis and cloned in pCRTM4Blunt-TOPO® Vector (Invitrogen cat # K2875-20) by TA cloning ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA quality was assessed by denaturing agarose gel electrophoresis and quantified by the Qubit RNA BR Assay Kit (ThermoFisher). For library preparation ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA concentration was measured by gel electrophoresis (5µl sample on 1.2% agarose) and by spectrophotometry through Nanodrop 3300 (Thermo Fisher Scientific).
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bioRxiv - Synthetic Biology 2022Quote: ... Samples of elution fractions were taken to perform sodium dodecyl sulfate polyacrylamide agarose gel electrophoresis (SDS-PAGE) (Bolt 4-12% Bis-Tris Plus, Invitrogen) at 180 V for 30 min in 1× MES buffer (Novex ...
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bioRxiv - Biophysics 2022Quote: SDS-PAGE electrophoresis was conducted by using Novex® NuPAGE® SDS-PAGE Gel System (Life Technologies, Carlsbad, CA, USA). NuPAGE® 4–12% Bis-Tris gels (Life Technologies ...
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bioRxiv - Biophysics 2022Quote: ... 12% polyacrylamide-gel electrophoresis (PAGE) gels in 1x TBE running buffer and were visualized by staining with SYBR Gold II (Invitrogen) stain or fluorescence imager.
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bioRxiv - Physiology 2022Quote: ... Lysates were separated by SDS–PAGE using the XCell SureLock Electrophoresis Cell on NuPAGE Novex 4%–12% Bis-Tris Protein Gels (Invitrogen). Proteins were transferred onto polyvinylidene fluoride membranes (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... PCR products were purified by preparative agarose gel electrophoresis and extracted with GeneJET Gel Extraction Kit purchased from Thermo Fisher Scientific ...