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Citations for Thermo Fisher :
601 - 650 of 1252 citations for Vertical Electrophoresis Comb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... They were then resolved by SDS-PAGE electrophoresis using bis-tris NuPage gels as per manufacturer’s instructions (Invitrogen) and transferred to PVDF membranes which was blocked using 3% BSA dissolved in TBST (50 mM Tris [pH8.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... Gel electrophoresis was run at 120 V (constant) for 60 minutes in 1X MOPS SDS running buffer (ThermoFisher) using the Bolt Mini Blot Module (ThermoFisher ...
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bioRxiv - Biochemistry 2024Quote: ... equal volumes of viral lysates separated by electrophoresis on Bolt 4–12% Bis-Tris Plus gels (Life Technologies) were transferred using Trans-Blo Turbo Mini PVDF Transfer Packs (Bio-Rad) ...
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bioRxiv - Bioengineering 2024Quote: Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was conducted using 4 - 12 % NuPAGE gels (Thermo Fisher Scientific). Prior to SDS- PAGE ...
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bioRxiv - Genetics 2024Quote: ... PCR products were purified by gel electrophoresis and subsequent extraction using the GeneJet Gel Extraction Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... snap-cooled and separated by capillary electrophoresis (CE) at 12kV for 25min using a POP-6 polymer (Thermo Scientific). “One-pot” U-insertion/U-deletion reactions were assembled by annealing of the U-insertion and U-deletion pre-mRNA/gRNA-hybrid RNAs in separate reaction vials ...
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bioRxiv - Genetics 2021Quote: ... STR Multiplex fragment analysis was carried out on the 16-capillary electrophoresis system ABI 3300 Genetic Analyser (Applied Biosystems).
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bioRxiv - Microbiology 2019Quote: ... Blue native electrophoresis of the concentrated complexes was carried out on a 3-12% Bis-Tris gel (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Protein separation was conducted by electrophoresis in a Mini Gel Tank on 10% Tris-Glycine pre-cast gels (Invitrogen™ Novex™ Value™ 10% ...
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bioRxiv - Molecular Biology 2021Quote: ... The libraries were selected from 250–350-bp fragments obtained by E-Gel 2% SizeSelect electrophoresis (Invitrogen, cat# G661012). Size-selected libraries were verified using the Agilent 2100 Bioanalyzer with the High Sensitivity DNA kit (Agilent ...
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bioRxiv - Bioengineering 2022Quote: ... PCR product was analyzed by gel electrophoresis on a 0.8% agarose gel stained with SybrSafe and compared to a one kb Plus Ladder (Invitrogen). RT-PCR amplifications was performed for all the samplings harvested ...
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bioRxiv - Cell Biology 2022Quote: ... The lysates were then subjected to SDS gel electrophoresis before being transferred to nitrocellulose membranes using iBlot2 (Life Technologies), blocked with 5% BSA in TBS with 0.1% Tween-20 and incubated with primary antibodies ...
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bioRxiv - Genomics 2020Quote: ... The integrity and purity of the RNA were determined by gel electrophoresis and a NanoDrop ND-1000 (Thermo Scientific), respectively ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were verified and quantified by agarose gel electrophoresis with a standard low DNA mass ladder (Invitrogen). Purification and OneShot Sanger sequencing of 16S rRNA gene products was performed by Genoscreen (Lille ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Amplicons were visualized on 0.8% agarose gel electrophoresis using 1× TAE buffer stained with SYBRSafe (Invitrogen, Carlsbad CA, USA), and compared to a GeneRuler 1 Kb Ladder (ThermoScientific ...
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bioRxiv - Genetics 2020Quote: ... Quality/quantity of the extracted DNA was evaluated by gel electrophoresis and Qubit dsDNA BR Assay Kit (Life Technologies). To evaluate the probability of DNA degradation ...
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bioRxiv - Genomics 2020Quote: ... Products were visualized on a 2% E-Gel EX agarose gel run on a Power Snap Electrophoresis Device (ThermoFisher) to verify size ranges and quantified using a Qubit 2.0 Fluorometer and dsDNA HS Assay Kit (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products were verified and quantified by agarose gel electrophoresis with a standard low DNA mass ladder (Invitrogen). Purification and OneShot Sanger sequencing of merA and merAB gene products was performed by Genoscreen (Lille ...
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bioRxiv - Neuroscience 2021Quote: ... extracted nuclear proteins were subjected to NuPAGE Bis-Tris (4 to 12%) gel electrophoresis under reducing conditions (ThermoFisher Scientific) and then electrophoretically transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed with 3-8% Tris-Acetate gels (Invitrogen, Carlsbad, CA), as described previously [27] ...
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bioRxiv - Biochemistry 2020Quote: ... The purity of the EMX1 sgRNA was confirmed by electrophoresis on a 12% TBE-UREA-PAGE gel (Thermo Scientific). Concentrations were determined by A260nm using a NanoDrop instrument (Thermo Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... Electrophoresis was performed and proteins were dry transferred to a nitrocellulose membrane using an iBlot 2 system (ThermoFisher Scientific). The blots were probed with anti-phospho-AKT (Ser473 ...
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bioRxiv - Biochemistry 2021Quote: ... The gels were run at 4 °C using the electrophoresis system according to the Invitrogen instructions (ThermoFisher Scientific, USA), and then stained using the Pierce Silver Staining kit according to the instructions (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were separated by electrophoresis under denaturing conditions on NuPage Bis-Tris Pre-Cast gels (Invitrogen, Carlsbad, CA) for 60 min at 200 Volts and transferred onto nitrocellulose membranes (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Fragment analysis was done by capillary electrophoresis using LIZ-500 as internal size standard (Gene Scan 500, Applied Biosystems) and the typing data was analyzed by using BioNumerics software version 7.1 (Applied Maths ...
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bioRxiv - Microbiology 2021Quote: ... and RNA integrity was assessed with gel-electrophoresis and quantified using a Nanodrop (Thermo Fisher Science, Waltham, MA, USA). Library were prepared and singleend 50 bp sequenced on the DNBseq platform at BGI (Hong Kong ...
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bioRxiv - Microbiology 2021Quote: ... After analysis through electrophoresis on a 1% agarose gel as well as a Qubit 2.0 (Thermo Scientific, Waltham, USA), a NEBNext Ultra™ ‖ DNA Library Prep Kit (NEB ...
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bioRxiv - Microbiology 2020Quote: ... Protein quality and quantities were checked by SDS-PAGE electrophoresis (12% polyacrylamide gels) and UV spectrometry (Nanodrop, Thermo Scientific). A total of 100 μg protein from each condition was subjected to in-solution trypsin digestion before labelling the resultant tryptic peptides using the TMT-6plex Isobaric Label Reagent Set (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed using precast 4-12% polyacrylamide gradient gels (ThermoFisher, NW04122BOX), submerged at 4°C in Bolt™ MOPS SDS Running Buffer (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Gel electrophoresis was performed at 130 V for 70 min in Bolt MOPS SDS Running Buffer (ThermoFisher Cat# B0001). Proteins were subsequently transferred to a 0.45 μm polyvinylidene difluoride membrane ...
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bioRxiv - Plant Biology 2021Quote: ... PCR fragments were isolated from 1 % agarose gel after electrophoresis and purified by GeneJET Gel Extraction Kit (Thermo Fisher). The fragment generated was inserted into the PJET1.2/blunt cloning vector (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... The harvested DNA was detected by the agarose gel electrophoresis and quantified by Qubit 3.0 (Thermo Fisher Scientific, USA). Whole-genome sequence determinations of strain CS1 were carried out with the PacBio (Pacific Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... Equal quantities of cellular DNA from different conditions of the experiments were subjected to gel electrophoresis in 1.4% agarose gel with SYBR safe dye (Invitrogen). Agarose gel electrophoresis was done at 50V (5V/cm ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μl of proteins obtained after TCA precipitation were separated by 4-12% polyacrylamide gradient gel electrophoresis (NuPAGE-Invitrogen) in 1X MES buffer (50 mM MES ...
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bioRxiv - Immunology 2022Quote: ... The quality and quantity of RNA were evaluated by agarose gel electrophoresis and Qubit RNA HS Assay Kit (Invitrogen).
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bioRxiv - Microbiology 2022Quote: ... RNA quality and quantity were assessed using gel electrophoresis and using a NanoDropTM 2000/2000c Spectrophotometer (Thermo Fisher Scientific). All RNA extractions were carried out in triplicate.
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bioRxiv - Genetics 2019Quote: ... The quality and concentration of the extracted DNA was verified using gel electrophoresis and Qubit 2.0 fluorometer (Life Technologies). Extracted genomic DNA was used for preparing Illumina RAD sequencing libraries based on previously described protocols (Baird et al ...
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bioRxiv - Cancer Biology 2019Quote: ... Equal amounts of protein (30-50 µg) were fractionated on 4-12% gradient SDS-polyacrylamide gel electrophoresis gel (Invitrogen) and transferred to PVDF membrane (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... The integrity of DNA was checked on gel electrophoresis and quantified using a NanoDrop ND1000 Spectrophotometer (Thermo Scientific, USA).
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bioRxiv - Neuroscience 2020Quote: ... to be subjected to electrophoresis at 21 V for 30 min and stained in 2.5 μg/mL propidium iodide (Cat# P3566, Invitrogen) for 20 min ...
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bioRxiv - Immunology 2021Quote: ... Samples were resolved by electrophoresis on 4-12% Bis-Tris gels with MOPS Running Buffer (Life Technologies NuPAGE system) and transferred to nitrocellulose membrane by electrophoresis at 100V for 1 hour in cold transfer buffer (Life Technologies ...
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bioRxiv - Developmental Biology 2019Quote: ... RNA quality was tested using agarose gel electrophoresis and concentration was quantified using a Nanodrop Lite spectrophotometer (Thermo Fisher). Complementary DNA was created by reverse transcription using an iScript cDNA kit (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: ... loaded on leaf area basis and separated by polyacrylamide gel electrophoresis (Nu-PAGE 4-12% Bis-Tris gel, Invitrogen, Life Technologies Corporation ...
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bioRxiv - Genetics 2020Quote: ... Whole cell protein samples were resolved by SDS-PAGE electrophoresis and analyzed by Western blot using anti-V5 (1:40,000) (Invitrogen) with goat anti-mouse HRP (1:40,000) ...
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bioRxiv - Plant Biology 2021Quote: ... and separated by polyacrylamide gel electrophoresis (Nu-PAGE 4-12% Bis-Tris gel, Invitrogen, Life Technologies Corporation, Carlsbad, CA). Proteins were transferred to a nitrocellulose membrane and probed against photosynthetic proteins with antibodies according to manufacturer’s protocols (Agrisera ...
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bioRxiv - Genomics 2021Quote: The integrity and concentration of RNA and DNA was analyzed by agarose gel electrophoresis and Qubit 2.0 fluorometer (ThermoFisher), respectively ...
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bioRxiv - Biochemistry 2021Quote: ... Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) was performed on a NuPAGE Novex Bis-Tris gel following manufacturer’s instructions (Invitrogen). Protein/ACE2 or protein/mAb attached on magnetic beads or protein control in solution were mixed with 4X Sample Buffer and deionized water (1:3 v/v ...
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bioRxiv - Genomics 2021Quote: ... The quality and quantity of the DNA were measured via standard agarose gel electrophoresis and with a Qubit□4.0 Fluorometer (Invitrogen). Sequencing library was constructed and sequenced by Nanopore PromethION platform (Oxford Nanopore Technologies) ...
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bioRxiv - Genomics 2021Quote: ... The quality and quantity of the DNA were measured via standard agarose gel electrophoresis and with a Qubit□2.0 Fluorometer (Invitrogen). Sequencing library was constructed and sequenced by Illumina Novaseq platform (Illumina).
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bioRxiv - Genetics 2021Quote: ... were taken to reducing sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE; Gels: ThermoFisher NuPAGE 4 to 12 %, Bis-Tris). The separated proteins were transferred to nitrocellulose membranes (0.2 µm ...