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Citations for Thermo Fisher :
351 - 400 of 520 citations for Vancomycin HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Control (vehicle: 40 μM HCL, 0.002% BSA [0-5 d]; 0.1% DMSO [5-10 d]; all from Thermo Fisher Scientific) for 0-10 d [=Baseline] ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were resuspended in 1 ml IP lysis buffer [25 mM Tris-HCl pH 7.5 / 150 mM NaCl / 1% NP-40 / 1 mM EDTA / 5% glycerol / Halt protease inhibitor cocktail (Thermo scientific) / Halt phosphatase inhibitor cocktail (Thermo scientific)] and incubated on ice for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... mouse BM-MSCs were first transfected with lentiCas9-Blast vector (Genomeditech) and selected with 5 μg/ml blasticidin S HCl (A1113903, Invitrogen). Then ...
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bioRxiv - Microbiology 2019Quote: ... The eluate was dialyzed with sizing buffer (50mM Tris-HCl pH 7.5, 150mM NaCl, 1mM Dithiothreitol) in 10K MWCO SnakeSkin dialysis tubing (Thermo Scientific), and the His6-Sumo-tag was cleaved with 1 μL SUMO protease per 100 μg of protein ...
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bioRxiv - Microbiology 2020Quote: ... The reaction was stopped by addition of 0.5 N HCl and absorbance at 450 nm was measured using a Muktiskan FC microplate photometer (Thermo Scientific).
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bioRxiv - Genomics 2019Quote: ... Cells were resuspended in 1 ml IP lysis buffer [25 mM Tris-HCl pH 7.5 / 150 mM NaCl / 1% NP-40 / 1 mM EDTA / 5% glycerol / Halt protease inhibitor cocktail (Thermo scientific) / Halt phosphatase inhibitor cocktail (Thermo scientific)] and incubated on ice for 1 h ...
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bioRxiv - Genetics 2019Quote: Neuro2a cells were thawed and protein extraction was performed with RIPA buffer (25mM Tris•HCl pH 7.6, 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS; Thermo Fisher 89900) supplemented with protease inhibitors (Sigma P8849) ...
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bioRxiv - Biochemistry 2020Quote: ... 5 or 7.5 µg aliquots of each in vitro transcript was refolded in 100 µL lysis buffer (20 mM Tris-HCl (pH 7.5, Ambion, AM9850G, and Ambion, AM9855G), 150 mM NaCl (Ambion ...
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bioRxiv - Developmental Biology 2020Quote: ... 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol; Thermo Fisher) containing 1x protease inhibitor cocktail III (Calbiochem ...
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bioRxiv - Genomics 2021Quote: ... the nuclei pellet was resuspended in 50 µl of transposase master mixture (25µl of 2X TD buffer (20mM Tris-HCl pH 7.6 (Invitrogen, 15568-025), 10mM MgCl2 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1 M MgCl2, 0.1M Tris HCL, 1% Tx-100, 1x sBlock, 1% sodium deoxycholate, 5 units/mL DNAse I – Thermo Scientific #89836 ...
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bioRxiv - Systems Biology 2021Quote: ... Native protein complexes from platelets were obtained by incubating the washed pallet with Pierce IP lysis buffer (25 mM Tris•HCl pH 7.4, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 5% glycerol) (Thermo Scientific) containing protease and phosphatase inhibitors at 4 °C for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... eluted in Tris–EDTA buffer (10mM Tris–HCl, 0.1mM EDTA, pH 8.5) and quantified with a Nanodrop ND-1000 spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... quenched in 100 mM Tris-HCl pH 8.0 and analyzed on a NuPAGE™ 3–8 % Tris-Acetate gel (Invitrogen). Fractions containing protein bands corresponding to the expected size of a Pol II–APT complex were dialyzed against 10 mM K-HEPES pH 8.0 ...
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bioRxiv - Genomics 2022Quote: ... MB135-iFLAG-SRSF3-FL and -TR myoblasts were additionally maintained in 10 μg/mL blasticidin S HCl (Thermo Fisher Scientific). Induction of DUX4 and SRSF3 transgenes was achieved by culturing cells in 1-2 μg/mL doxycycline hyclate (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were diluted 4x by addition of 3ml of regular lysis buffer (50mM Tris-HCl pH8, 100mM NaCl, 0.5% NP40, 2mM EDTA, 1 tablet of phosphatase/protease inhibitor (Thermo Scientific) per 10ml of lysis buffer) ...
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bioRxiv - Synthetic Biology 2023Quote: ... HEK293S GnTI- TetR cells were routinely cultured in growth medium with the addition of 1 μg/mL blasticidine S HCl (11583677, Gibco) for selection of the TetR trait.
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bioRxiv - Molecular Biology 2023Quote: ... 100 mL Lysis Buffer (10 mM Tris-HCl, 1 mM EDTA [pH 8], 2.75 mM DTT, Halt protease inhibitors (ThermoFisher Scientific), and a 45-s process in a FastPrep-24 (MP Biomedicals ...
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bioRxiv - Biophysics 2023Quote: ... the cell pellets were solubilized and incubated at 25°C for an hour in lysis buffer (500 mM NaCl, 50 mM Tris HCl pH 7.5, 10 mM Imidazole, 25% B-PER solution (Thermofisher, 90084), 2.5 mg/mL lysozyme (Thermofisher ...
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bioRxiv - Molecular Biology 2023Quote: ... were incubated in 20 mL 33 loading buffer diluted to 13 in lysis buffer (10 mM Tris-HCl, 1 mM EDTA [pH 8], 2.75 mM DTT, Halt protease inhibitors (ThermoFisher Scientific)) ...
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bioRxiv - Microbiology 2023Quote: ... Lysed cells were resuspended in 500µL of Sonication buffer (SDS 1%, 10mM EDTA, 50mM Tris HCl pH 8) with an anti-protease cocktail (A32965, ThermoFisher) and sonicated for 15” at 4°C (30s ON ...
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bioRxiv - Cancer Biology 2023Quote: Meningioma cells for immunoblotting were lysed in 1% SDS in 100mM pH 6.8 Tris-HCL containing protease and phosphatase inhibitor (A32961, Thermo Scientific), vortexed at maximum speed for 1min ...
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bioRxiv - Neuroscience 2023Quote: ... Sulfated agarose product or commercially available agarose or agar powder was dissolved in 0.5M HCl at a concentration of 5% (w/v) in 2mL screw-top microtubes with gasket (Fisher Scientific). Tubes are then placed in a heat block at 100-110°C for 3hours with periodic vortex mixing ...
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bioRxiv - Biophysics 2023Quote: ... SDS-PAGE analyses were performed at 180V for 75 min using precast 4-20% Tris-HCl polyacrylamide gradient gels (ThermoFisher). In-gel fluorescence was detected using a ChemiDoc MP imaging system (Bio-Rad).
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bioRxiv - Genomics 2023Quote: ... The sample was then imaged with an anti-bleaching buffer consisting of 50 mM Tris-HCl pH 8.0 (Invitrogen 15568025), 4× SSC ...
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bioRxiv - Plant Biology 2023Quote: ... Additional Tris-HCl was added to the coverglass which was then put into an Attofluor Cell Chamber (ThermoFisher, Cat #A7816). A larger area was imaged via spinning disk microscopy by acquiring a 3 x 3 tiled Z stack in 0.01 nm z-interval steps to account for cover glass levelness ...
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bioRxiv - Immunology 2023Quote: ... were aliquoted to 1.5μL per sample and equilibrated by three washes in IP lysis buffer (50mM Tris/HCl, 0.5% NP40, complete protease inhibitor cocktail (ThermoFisher, #A32955), pH=7.4) ...
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bioRxiv - Microbiology 2023Quote: ... 50 mM KCl, 100 mM Tris-HCl [pH 7.4], 40% glycerol, 0.4 U/µl of Superase IN [Thermo Fisher Scientific]) and incubated at room temperature for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The protein samples were denatured by adding 3× SDS sample buffer (containing 188 mM Tris-HCl, pH 6.8; 3% SDS; 30% glycerol; 0.01% bromophenol blue; and 1× Bond-Breaker TCEP solution from Thermo Fisher Scientific) and heating at 95°C for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... using elvanol containing antifade (polyvinyl alcohol, Tris-HCl pH 8.5, glycerol and DABCO, catalog number 11247100, Fisher Scientific, Hampton, NH). All wash and incubation steps were performed on a nutator ...
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bioRxiv - Cell Biology 2023Quote: Cells were washed twice with PBS and lysed with RIPA buffer (25 mM Tris-HCl, pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS; Thermo Fisher; 89901) supplemented with 1 mM EDTA (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... 200 mM Tris-HCl, pH 8.0, 8 M Urea, 4% CHAPS, 1 M NaCl, supplemented with protease and phosphatase inhibitors, Thermo Scientific) was added to the pellets and the extracts were sonicated 10 x 3s with a probe sonicator ...
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bioRxiv - Molecular Biology 2023Quote: Cells were washed with DPBS and lysed with 160 μL RIPA buffer (25 mM Tris-HCl pH 7.5 (Invitrogen 15567027), 150 mM NaCl (Invitrogen AM9760G) ...
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bioRxiv - Cell Biology 2024Quote: ... After 5-10 mins of washing with 2xSSC buffer, samples were mounted in freshly prepared GLOX mounting medium (0.4 % glucose; 10nM Tris–HCl, pH 8.0 (Invitrogen, Cat.#AM9856) and 2× SCC ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein was isolated from these brains using RIPA buffer (25 mM Tris HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS; Thermo Scientific #89900) supplemented with 1X Protease Inhibitor Cocktail (Roche # 5892791001 ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was performed using 4 µl 5× reaction buffer (250 mM Tris-HCl [pH 8.3 at 25 °C], 250 mM KCl, 20 mM MgCl2, 50 mM DTT [ThermoFisher Scientific]), 0.5 µl ribolock RNase inhibitor (40 U/µl ...
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bioRxiv - Genomics 2023Quote: ... Reverse transcription mix which contained 0.4 μL of 5X M-MLV reverse transcriptase buffer (250 mM Tris-HCl, 375 mM KCl, 15 mM MgCl2, Invitrogen), 0.1 μL of 0.1 M DTT (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... All fractions were neutralized with Tris-HCl (0.1 M; pH 9.0) and their protein concentrations were determined by bicinchoninic acid (BCA) assay (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... initial denaturation at 72°C for 10min was performed followed by the addition of 1µL RT mix consisting of 25mM Tris-HCL pH 8.4 (Fisher Scientific, #NC1584154), 30mM NaCl (Ambion ...
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bioRxiv - Cell Biology 2023Quote: Proteins were obtained from 30 wing discs lysed in a lysis buffer: (50nM Tris-HCl pH8; 150nM NaCl, 1% NP40 and anti-protease + anti phosphatase INVITROGEN) and frozen at -20°C.
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bioRxiv - Genomics 2024Quote: ... and nuclei were washed once with nuclei suspension buffer (NSB; 10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 1% Superase RNA Inhibitor (Thermo Fisher), 1% 0.2mg/mL Ultrapure BSA (New England Biosciences)) ...
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bioRxiv - Microbiology 2024Quote: ... The eluted proteins were dialysed overnight in 20 mM Tris-HCl buffer (pH 8) supplemented with 1 mM DTT using a 10 kDa MWCO SnakeSkin dialysis tube (ThermoFisher) and loaded onto a MonoQ 5/50 GL column for anion exchange chromatography ...
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bioRxiv - Microbiology 2024Quote: ... The eluted proteins were dialysed overnight in 20 mM Tris-HCl buffer (pH 8) supplemented with 1 mM DTT using a 10 kDa MWCO SnakeSkin dialysis tube (ThermoFisher), then concentrated to ∼0.5 mL and loaded onto a Superose 6 Increase 10/300 column pre-equilibrated in GF150 buffer ...
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bioRxiv - Microbiology 2020Quote: ... The reaction was stopped by the addition of 50μl/well 10% HCl and the absorbance was measured at 492nm (A492) using a Multiskan FC reader (Thermo Fisher Scientific). In every CovIgG-Assay determination two in-house controls ...
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bioRxiv - Biophysics 2021Quote: ... 200 mg of each fecal pellet also used for DNA extraction were dissolved in 1 mL buffer (10 mM Tris-HCl, pH 8.0) containing 1X Halt™ protease inhibitor cocktail (Thermo Scientific). Cell lysis was achieved by bead beating in a Lysing Matrix E tube (MP Biomedicals ...
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bioRxiv - Biophysics 2020Quote: All the in vitro transcription experiments were performed in NEB’s transcription buffer (40 mM Tris-HCl, 6 mM MgCl2, 1 mM DTT, 2 mM spermidine) with 1 mM NTPs (R1481, Thermo Scientific), 22 wt% glycerol ...
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bioRxiv - Cancer Biology 2021Quote: Cells were gently washed twice in ice-cold 1X PBS and lysed using ice-cold cell lysis buffer (50 mM Tris-HCl, 2% w/v SDS, 10% glycerol) containing 1X HALT (dual phosphatase and protease inhibitor, Thermo Fisher). Lysates were sonicated at 20% amplitude for 20 seconds ...
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bioRxiv - Neuroscience 2021Quote: ... or 13C6 L-Lysine-2HCl (Lys-8) and 13C6 L-Arginine-Hcl (Arg-10) for the metabolic incorporation of ‘light’ and ‘heavy’ stable isotopes respectively (ThermoFisher Scientific). HEK293FT cells were grown in light or heavy SILAC media for at least 10 doublings ...
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bioRxiv - Molecular Biology 2020Quote: ... and diluted to 30 pg/μl concentration in 5 mM Tris-HCl (pH 7.5) supplemented with 5 ng/µl carrier herring sperm (Thermo Fisher Scientific). The MSI assay was performed using single-molecule PCR (SM-PCR ...
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bioRxiv - Neuroscience 2019Quote: ... then the supernatant was removed and the pellet was resuspended in in label-free buffer [100 mM Tris-HCl (pH7.6) 4% (w/v) SDS] containing 1% protease cocktail inhibitor (Thermo Fisher, UK). Homogenates were centrifuged at 20,000 x g for 20 minutes at 4°C with the soluble fraction of each sample transferred to Lo-Bind tubes (Sigma Aldrich) ...