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Citations for Thermo Fisher :
301 - 350 of 520 citations for Vancomycin HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... and gently resuspended in 250uL 1x Omni-ATAC Buffer (10mM Tris-HCl pH 7.5, 5mM MgCl2, 0.33x PBS, 10% DMF, 0.01% Digitonin (Thermo Fisher Scientific), 0.1% Tween-20) ...
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bioRxiv - Immunology 2022Quote: ... Raji-GFP-Luc cells were purchased from Biocytogen (B-HCL-010) and cultured in complete RPMI (21875, Thermo Fisher). Jurkat cells purchased from ATCC (TIB-152 ...
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bioRxiv - Molecular Biology 2023Quote: ... Twice-purified DNA was eluted into 10 μl of 10 mM Tris-HCl (pH 8.0) and quantified using the Qubit dsDNA HS Assay Kit (Invitrogen) with a Qubit 4 Fluorometer ...
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bioRxiv - Neuroscience 2023Quote: hiPSCs were lysed in lysis buffer (25mM Tris HCl pH 7.4, 62.5mM NaCl, 2% NP-40 (Thermofisher Scientific 85124), 1mM EDTA (Thermofisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... Tagemtation was performed by adding 4 µL 8x TD buffer (80mM Tris-HCl pH=7.6, 40mM MgCl2 (Invitrogen, #AM9530G), 80% N,N Dimethylformamide (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... the fixed cells were incubated with click-it reaction mix (100 mM Tris-HCl pH 8.5, 10 μM Azide-Alexa 647 (Invitrogen), 1 mM CuSO4 and 100 mM Ascorbic acid (Sigma Aldrich ...
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Neural circuit-wide analysis of gene expression during deafening-induced destabilization of birdsongbioRxiv - Neuroscience 2022Quote: ... waiting until solution cleared, removing the solution, adding 1 mL of TE Buffer (10 mM UltraPure Tris HCl, pH 8.0 (ThermoFisher), 1 mM EDTA pH 8 (ThermoFisher)) ...
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bioRxiv - Genomics 2022Quote: ... 25 µL streptavidin beads was washed by 1x BW buffer (5mM Tris-HCl pH=7.6, 0.5mM EDTA (Invitrogen #AM9260G), 1M NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... Cortical samples were stored at -80 °C until homogenization in RIPA buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS; Thermo Fisher) containing cocktails of protease and phosphatase inhibitors ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The striatal and hippocampal samples were stored at -80°C until homogenization in RIPA buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS; Thermo Fisher) containing cocktails of protease and phosphatase inhibitors ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice with ice-cold PBS before adding swelling buffer (10 mM Tris-HCL pH 7.5, 2mM MgCl2, 3 mM CaCl2, 2U/ml Superbase-in (Invitrogen)) ...
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bioRxiv - Genetics 2023Quote: ... using 100μl of freshly prepared lysis buffer (10 mM Tris-HCl, pH 7.5; 0.05% SDS; 25 μg/ml proteinase K (ThermoFisher Scientific) directly into each well of the tissue culture plate ...
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bioRxiv - Cell Biology 2023Quote: ... 10,000 cells were seeded in a 100 mm dish and selected with 5 µg/ml Blasticidin S HCl (Invitrogen). Colonies formed after 2-3 weeks were analyzed for anti-TET repressor by western blots (data not shown) ...
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bioRxiv - Neuroscience 2023Quote: ... 500 μL of washing buffer was added (Tris-HCL 10mM, NaCl 10 mM, MgCl2 3 mM, Tween-20 0.1%, BSA 1%, Invitrogen™ RNAseout™ recombinant ribonuclease inhibitor 0,04 U/μL ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated in TNB blocking buffer comprised of dH2O containing 0.1 M Tris-HCl (pH 7.5, Fisher Scientific), 0.15 M NaCl (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... and lysed in lysis buffer (20mM Tris HCl, 150mM NaCl, 5mM MgCl2, 1mM dithiotrietol, 0.05% NP-40, 25U/mL Turbo-DNase I (Invitrogen), 2ug/mL cycloheximide) ...
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bioRxiv - Genomics 2023Quote: ... Nuclei were pooled isolated with lysis buffer consisting of 10 mM Tris-HCl (pH 7.5, Invitrogen, cat. no. 15567027), 10 mM NaCl (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: Approximately 1 × 107 cells were resuspended in 0.5 ml cold TNE buffer (25 mMTris-HCl, pH 7.5, 150 mM NaCl, 5 mM EDTA, and 1% Triton X-100; Surfact-Amps, ThermoFisher) with a tablet of protease inhibitors (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... Parasite nuclei were thereafter collected and resuspended in shearing buffer (1% SDS, 10 mM EDTA, 50 mM Tris HCl pH 8.1 and 1 x Halt protease inhibitor cocktail, ThermoFisher). Chromatin was fragmented using the Covaris ultra sonicator (ME220 ...
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bioRxiv - Cell Biology 2023Quote: Protein from 2×106 cells was harvested on ice after 1 PBS wash using the Pierce RIPA lysis and extraction buffer (25 mM Tris-HCl, pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, Life Technologies) containing fresh protease and phosphatase inhibitors (Life Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA mixture was deacylated (37°C for 45 minutes in 20 mM Tris-HCl pH 9.0, RNaseOUT (1 µg/µL, Invitrogen) and then 3’-end dephosphorylated and 5’-end phosphorylated using T4 polynucleotide kinase (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... from each sample were separated on 10% Tris-HCL SDS-PAGE gels and transferred to a nitrocellulose membrane (0.45 µm pore size, #LC2001, Invitrogen). Blots were blocked with fish gelatin (Biotium ...
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bioRxiv - Cell Biology 2024Quote: ... half of the media was replaced with fresh HoxB8 Media Culture containing 5 µg/mL blasticidin-HCl (ThermoFisher A1113903) for selection ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μl of reverse transcription mix (50 mM Tris-HCl, pH 8.3 [Sigma], 75 mM NaCl [Ambion], 1 mM GTP [Thermo Scientific], 3 mM MgCl2 [Ambion] ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μl of reverse transcription mix (50 mM Tris-HCl, pH 8.3 [Sigma], 75 mM NaCl [Ambion], 1 mM GTP [Thermo Scientific] ...
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bioRxiv - Developmental Biology 2021Quote: ... and 40 mM Tris-HCl at pH 8.0) and then treated with 1.5 U/50 mL of DNase I (Thermo Fisher Scientific) for 10 min at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... the PBS was replaced by a reducing and oxygen-scavenging buffer (Halpern et al., 2015) optimized for dSTORM blinking that consists of 100 mM Tris-HCl (Invitrogen), 10% (wt/vol ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction was quenched with 80 mM Tris-HCl (pH 8.0) for 10 minutes and then desalted using 7K MWCO Zeba desalting columns (Thermo Fisher). A 10-fold molar excess of the azide modified oligonucleotide was then incubated with the DBCO-antibody mixture overnight at 4 °C ...
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bioRxiv - Genomics 2020Quote: ... and 100 μL of freshly prepared lysis buffer was added (100 mM Tris-HCl, pH 7.0, 0.05% SDS, 25 μg/mL Proteinase K (Thermo Fisher Scientific). Transfection plates containing lysis buffer were incubated at 37 °C for 1 hour and the mixture was transferred to a 96-well PCR plate and heated at 80 °C for 30 min.
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bioRxiv - Molecular Biology 2019Quote: ... Each cDNA reaction was diluted by adding 100 μL buffer (10 mM Tris HCl pH 8.5. Transcription levels were quantified by qRT-PCR using a Life Technologies Vii7 system ...
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bioRxiv - Biochemistry 2019Quote: ... and lysed with NP-40 lysis buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1% NP-40 and protease inhibitor cocktail (Thermo Scientific)) ...
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bioRxiv - Systems Biology 2019Quote: ... 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol; Thermo Fisher) containing 1x protease inhibitor cocktail III (Calbiochem) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were rinsed with PBS and incubated with EdU staining buffer (150 mM Tris/HCl pH8.8, 1mM CuSO4, 100 mM ascorbic acid and 10 μM AlexaFluor azide (Life Technologies)) for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... in NTMT pH9.5 solution (100mM NaCl, 100mM Tris-HCl, 50mM MgCl2, 0.1% Tween20) and mounted with Aquamount (Thermo Fisher Scientific). Fluorescent substrate was revealed with Fast Red tablets (Kem-En-Tech ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were desalted into reaction buffer (50 mM Tris-HCl, pH 7.5, 500 mM NaCl and 4 mM Tris (2-Carboxyethyl) Phosphine (TCEP) (Invitrogen, T2556)) for removing DTT in storage buffer ...
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bioRxiv - Biochemistry 2020Quote: ... purified plasmid DNA was fractionated on a 10 – 40 % sucrose gradient in 20 mM Tris-HCl pH 7.5 / 1mM EDTA / 1M NaCl using an AH-629 swinging bucket rotor (Thermo Scientific) at 27,000 rpm for 20 hours at 20°C ...
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bioRxiv - Cell Biology 2021Quote: Cells were harvested by scraping in CHAPS lysis buffer (20mM Tris-HCl pH7.5, 500mM NaCl, 0.5% w/v CHAPS) with protease inhibitors (Thermo Scientific #A32965). Cells were pipetted up and down 30 times and left on the ice for 30 minutes ...
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bioRxiv - Genomics 2020Quote: ... The DNA was eluted in 150 μL 10 mM Tris-HCl pH 8 and the biotinylated molecules captured with Dynabeads M280 (Invitrogen) according to the manufacturer’s protocol but using150 μL Bead Binding buffer (Phase Genomics ...
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bioRxiv - Biochemistry 2020Quote: ... then lysed in hypertonic buffer B (10mM Tris-HCl pH 7.5, 800mM NaCl, protease and phosphatase inhibitors, and universal nuclease (ThermoFisher Scientific)) for 5 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... The pH 8.0 of 0.1M Tris-HCl buffer was adjusted for the medium and sterilized using 0.45 μm Nalgene syringe filter (Thermo Scientific, USA) separately ...
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bioRxiv - Genomics 2022Quote: ... The fragmented DNA samples were eluted with 10 μl of 10 mM Tris-HCl (pH 7.5) and quantified using a Nanodrop (Thermo Scientific). Samples were aliquoted into 1 ng ...
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bioRxiv - Molecular Biology 2022Quote: Dephosphorylation of 3’ ends was performed in 20 μl of PNK reaction for 30 minutes at 37°C (70 mM Tris-HCl, pH 6.5, 10 mM MgCl2, 1 mM DTT, 10U SUPERaseIN RNase Inhibitor (ThermoFisher, #AM2696), 5U T4 Polynucleotide Kinase (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... alkylated using iodoacetamide in 8M urea denaturation buffer (50mM Tris-HCl, pH 8) and digested overnight in 50mM ammonium bicarbonate using trypsin (ThermoFisher) at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... buffer (25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, and 0.1% SDS) (ThermoFisher Scientific, #89900) containing protease (MilliporeSigma ...
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bioRxiv - Microbiology 2020Quote: ... subsequently suspended in phage buffer (10 mM Tris-HCl, Sigma,10 mM MgSO4, 150 mM NaCl, pH 7.5, Fisher Scientific), and stored at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Frozen cell powder of a 250 mL culture was solubilized with 200 µL polysome lysis buffer (20 mM Tris-HCl pH 8.0 (Ambion, AM9855G), 140 mM KCl (Ambion ...
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bioRxiv - Microbiology 2021Quote: ... 50 mM KCl, 100 mM Tris-HCl [pH 7.4], 40% glycerol, 0.4 U/μL of Superase•IN [Thermo Fisher Scientific]) and incubated at 25 °C for 10 min ...
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bioRxiv - Developmental Biology 2021Quote: Cells and organoids were lysed in modified radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH 7.4, 0.2% SDS (Invitrogen, cat# 15553), and 0.1% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... The solution pH was adjusted to 7.4 by dropwise additions of 0.1 M HCl or NaOH solution and measured with a pH meter (Model 13-641-253, Thermo Scientific Orion 720A+ ...
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bioRxiv - Cell Biology 2022Quote: ... 10 % glycerol and 50 mM Tris-HCl (pH 8.0)) supplemented with 1× EDTA-free Halt protease inhibitor cocktail (ThermoFisher Scientific), 1 mM EGTA (pH 7,7) ...