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Citations for Thermo Fisher :
601 - 650 of 5824 citations for VAPB Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The inserts were released by Bam-HI and Eco-RI digestion and ligated to pENTR4 (Invitrogen; previously digested with the same restriction enzymes) ...
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bioRxiv - Cell Biology 2023Quote: ... The in vitro-translated proteins were then purified using His Pur cobalt spin columns (Thermo Scientific). For in vitro kinase assays ...
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bioRxiv - Biophysics 2022Quote: ... The His tagged RBD construct was purified from the culture supernatant of Expi293 cells (Thermo Fisher) that were transfected with the plasmid DNA using FectoPRO (Polyplus Inc.) ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were incubated 10 minutes at 4°C with Dynabeads™ His-Tag Isolation (Invitrogen, 10103D). After one wash with NLB buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The purified His-TEV-FAM19A5 proteins were digested overnight at 30°C with AcTEV protease (Invitrogen) to remove the His-tag and TEV sequence ...
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bioRxiv - Plant Biology 2023Quote: ... His-tagged AtKu70/80 was isolated from the supernatant using HisPur Ni-NTA resins (Thermo Fisher) and dialysed in 20 mM HEPES pH7.5 ...
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bioRxiv - Microbiology 2023Quote: ... brucei rRAP1-His at 10 µM was incubated with 10 mM SYPRO Orange dye (ThermoFisher Scientific), filtered before use with a 0.22 μm sterile filter (Fisher scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Cloning strategy for generating codon optimized SARS CoV-2 ORF7a in pCDNA-V5/His TOPO (Invitrogen) has been described previously (13) ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was incubated with an anti-His Rabbit primary antibody (Thermo Scientific, Massachusetts, United States) (1:1000) ...
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bioRxiv - Immunology 2023Quote: ... + 10% (v/v) HI-FBS + 100 U/ml penicillin-streptomycin (pen-strep, ThermoFisher Gibco™ #15140122) at 37°C ...
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bioRxiv - Immunology 2023Quote: ... + 10% (v/v) HI-FBS + 100 U/ml penicillin-streptomycin (pen-strep, ThermoFisher Gibco™ #15140122) at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... The buffer was then exchanged to the wash solution (10 % Hi-DiTM Formamide (Thermofisher scientific; 4311320) in 2x saline sodium citrate (SSC) ...
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bioRxiv - Cancer Biology 2020Quote: ... using Taqman probes (human RBM10: Assay ID, Hs00275935_m1, human Bcl-xL: Assay ID, Hs00236329_m1, Applied Biosystems, human Bcl-xS ...
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bioRxiv - Biochemistry 2022Quote: ... Human serum (catalog # 4522 from human male AB plasma) and fetal bovine serum were from Gibco, Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... and plasma human glucagon was measured by the human glucagon ELISA kit (Thermo Scientific, Frederick, MD). Exendin-4 plasma levels were measured using the exendin-4 EIA kit (Phoenix pharmaceuticals ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 50 μL fresh FACS buffer and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Immunology 2022Quote: ... Total IgG in human sera was measured using a commercial human IgG ELISA kit (Fisher Scientific) according to manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... All human CD8+ T cells were activated with anti-human CD3/CD28 dynabeads (11131D, Thermo Scientific) at a 1:1 cell-to-bead ratio and cultured in complete RPMI supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... 100 ng/mL Human Recombinant IL-4 and 250 ng/mL Human Recombinant GM-CSF (ThermoFisher) for six days at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... Fragments of human CCDC22 and CCDC93 and full-length human DENND10 were ordered as GeneStrings (ThermoFisher) with the gene codon optimized for E ...
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bioRxiv - Cell Biology 2023Quote: ... Purified human neutrophils and T cells were cultured in Human Plasma-Like Medium (HPLM; ThermoFisher Scientific) with 5% heat inactivated FBS supplemented ...
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bioRxiv - Immunology 2023Quote: ... Primary human T cells were thawed and activated with Human T-Expander CD3/CD28 Dynabeads (Gibco) at a 3:1 bead:cell ratio in complete medium (RPMI 1640 supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2023Quote: ... Human Jurkat T cells and human THP-1 monocytes were cultured in RPMI 1640 medium (ThermoFisher), supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: Fresh human BAL cells and frozen human PBMCs were stained with Aqua live/dead dye (Invitrogen) for 20min at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 50 µL fresh FACS buffer and 10 µL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 50 µL fresh FACS buffer and 10 µL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Neuroscience 2023Quote: ... Secreted Aβ42 peptides were quantified using the Human Amyloid β1-42 human ELISA kit (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... human melanocyte growth supplement (HMGS, Gibco), gentamicin (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... Pool A (human) (Thermo Fisher Scientific) using the TaqMan® microRNA Reverse Transcription Kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... human skeletal myoblasts (Thermo Fisher Scientific), and mouse C3H muscle myoblasts (C2C12 cells ...
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bioRxiv - Cancer Biology 2019Quote: ... human keratinocyte growth supplement (ThermoFisher Scientific), and penicillin-streptomycin ...
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bioRxiv - Cell Biology 2019Quote: Episomal human iPSCs30,31 (Thermo Fisher Scientific) were maintained as a monolayer on Matrigel (Corning Life Sciences ...
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bioRxiv - Cancer Biology 2020Quote: ... with human melanocyte growth supplements (Gibco). Cell lines were incubated at 37 °C in 5% CO2.
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bioRxiv - Molecular Biology 2019Quote: ... Human colon total RNA (Thermofisher Scientific) was aliquoted at 13 µg/mL and stored at −20°C
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bioRxiv - Cancer Biology 2021Quote: Human melanocytes were trypsinized (Gibco, 25300062), quenched ...
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bioRxiv - Systems Biology 2021Quote: ... The human 62-plex (eBiosciences/Affymetrix) was utilized with the modifications described below ...
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bioRxiv - Bioengineering 2020Quote: ... PE-conjugated anti-human CXCR4 (Invitrogen), FITC-conjugated anti-human ACE2 (R&D Systems) ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant human TGF-β1 (Life Technologies) was prepared in sterile water with 0.1% BSA following the manufacturer’s instructions and diluted in 50:50 media.
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bioRxiv - Genomics 2021Quote: ... Human neural stem cells (NSCs) (Gibco, a kind gift from Raymond Poot ...
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bioRxiv - Cell Biology 2021Quote: ... and human ATF4 (Ambion, USA, 16708), and Scrambled (Negative control ...
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bioRxiv - Microbiology 2020Quote: Normal Human Dermal Fibroblasts (NHDF; Gibco) and IRF3 -/- cells were maintained in Dulbecco’s modified high glucose media (DMEM ...
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bioRxiv - Neuroscience 2020Quote: ... goat anti-human (ThermoFisher, 1:1000). Hoechst staining (ThermoFisher ...
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bioRxiv - Microbiology 2019Quote: Normal human dermal fibroblast (NHDF, Gibco) cells were maintained in Dulbecco’s modified high glucose medium (DMEM ...
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bioRxiv - Immunology 2020Quote: ... goat anti-human IgM (Invitrogen #A18835), or goat anti-human IgA (Jackson #109-036-011 ...
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bioRxiv - Biochemistry 2020Quote: ... CD45 (Human CD45 FITC Conjugate, Invitrogen), CD90 (PE Mouse Anti-Human CD90 BD Pharmingen ...
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bioRxiv - Neuroscience 2020Quote: ... human epidermal growth factor (Life Technologies), human insulin-like growth factor (Life Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... human fibroblast growth factor (Life Technologies), human epidermal growth factor (Life Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng/mL human EGF (Invitrogen, 15630-056 ...
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bioRxiv - Cancer Biology 2021Quote: ... human keratinocyte growth supplement (MEPICF500, Gibco), and 1% penicillin-streptomycin (PSL02-6X100ML ...
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bioRxiv - Cell Biology 2020Quote: ... Normal human dermal fibroblasts (NHDF - GIBCO) were maintained in fibroblast medium (DMEM high glucose ...