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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2022Quote: ... and RNA was extracted using a hybrid of Trizol (Ambion) and RNeasy mini kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... and hybrids were purified using MyOne-C1 Streptavidin Dynabeads (Invitrogen). Ligated probes were eluted by treatment with RNase H and RNase A ...
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bioRxiv - Microbiology 2019Quote: ... Two hundred nanograms of DNase-treated RNA was reverse transcribed into cDNA using SuperScript™ III First-Strand Synthesis System (Invitrogen). Quantitative PCR was carried out in a 10-μL volume using PowerUp SYBR Green Master Mix (Applied biosystems ...
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bioRxiv - Genomics 2020Quote: Two micrograms of RNA were used to synthesize first-strand cDNA with random hexamer primers and Superscript II™ (Invitrogen, CA). For NM_000701.8//XR_002956654.1 of ATP1A1 detection ...
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bioRxiv - Plant Biology 2021Quote: ... A total of two µg of total RNA was used to prepare cDNA by using SuperScript III First-Strand Synthesis System (Invitrogen, USA). Samples were pre-treated with TURBO DNA-free kit to remove genomic contamination (Ambion ...
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bioRxiv - Biochemistry 2021Quote: ... Two μg of total RNA were subjected to reverse transcription using High-Capacity cDNA Reverse Transcription Kit (Thermo Scientific, Rockford, IL) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... purified RNA was amplified using the SuperScript GeneChip Expression 3’-Amplification Reagents Two-Cycle cDNA Synthesis Kit (Affymetrix, Santa Clara, CA) and the GeneChip T7-Oligo Primer (Affymetrix ...
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bioRxiv - Cancer Biology 2020Quote: ... Two microliters of diluted cDNA was added to a master mix of 10 μL of SYBR Green (ThermoFisher Scientific, CAT# 4385612), 1 μL each of 10 μM forward and reverse primer ...
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bioRxiv - Microbiology 2020Quote: We initially used a two-step method where viral RNA was converted into cDNA using Invitrogen SuperScript IV VILO Master Mix (11766500, Thermo Fisher) in 96-well format (18021-014 ...
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bioRxiv - Biochemistry 2019Quote: RNA was extracted from cells with Machery-Nagel NucleoSpin RNA II Kit (Biotop Oy, Turku, Finland) and two µg was transcribed to cDNA using RevertAid reverse transcriptase (Thermo Scientific) and random hexameric primers ...
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bioRxiv - Physiology 2021Quote: ... Two micrograms of RNA from each tissue sample were reverse-transcribed using the high-capacity cDNA reverse transcription kit (Applied Biosystems). The resulting cDNA products were diluted 1:20 and subjected to qPCR reactions using TaqMan reagents ...
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bioRxiv - Cell Biology 2023Quote: ... Two μg of RNA were reverse transcribed into cDNA using SuperScript™ and 10 mM dNTP Mix (both Thermo Fisher Scientific) and oligodT (Sigma Aldrich) ...
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bioRxiv - Plant Biology 2023Quote: ... Two μg of total RNA were subjected to reverse transcription using the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific, MA) with oligo(dT ...
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bioRxiv - Genomics 2023Quote: ... Two micrograms of RNA were used to synthesize the first-strand cDNA with random hexamer primers and Superscript IITM (Invitrogen, CA). One microliter of each cDNA sample was used for TaqMan PCR (Eppendorf RealPlex Mastercycler and Applied Biosystems QuantStudio 3 ...
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bioRxiv - Neuroscience 2024Quote: ... Two micrograms of total RNA were reverse transcribed using High-Capacity RNA to cDNA kit (Applied Biosystems, Rhenium, Modi’in, Israel, #4368814). Quantitative PCR analysis was performed using or FAST SYBR Green master mix (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... total RNA was purified as described above and used to generate cDNA libraries using the first strand cDNA synthesis kit (Thermo Fisher, Waltham, MA, USA). RT-qPCR was performed on a Biorad CFX connect instrument (Biorad ...
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bioRxiv - Microbiology 2020Quote: ... Preparation of the eight libraries for high-throughput sequencing involved two separate PCR steps using Invitrogen Platinum Pfx DNA polymerase (Thermo Fisher). In the first set of reactions ...
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bioRxiv - Microbiology 2022Quote: ... 16S rRNA V4 amplicon libraries were prepared using 25 µg of DNA in two 50 µl PCRs using Phusion High-Fidelity DNA Polymerase (ThermoFisher, USA) with the following reaction conditions ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA libraries were generated from RNA using the Superscript III kit and random hexamer primers (Invitrogen). Quantitative real-time PCR was performed using SYBR Green reagents in a StepOnePlus system (Applied Biosystems ...
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PD-1 checkpoint blockade disrupts CD4 T cell regulated adaptive B cell tolerance to foreign antigensbioRxiv - Immunology 2021Quote: Final cDNA amplified library concentration and quantity was determined using a Qubit 4 Fluorometer (Invitrogen Technologies) and 2100 BioAnalyzer (Agilent) ...
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bioRxiv - Immunology 2021Quote: ... Full length cDNA and indexed sequencing libraries were analyzed using the Qubit 4 fluorometer (Thermo Fisher) and Agilent 2100 BioAnalyzer.
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bioRxiv - Immunology 2021Quote: Final cDNA amplified library concentration and quantity was determined using a Qubit 4 Fluorometer (Invitrogen Technologies) and 2100 BioAnalyzer (Agilent) ...
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bioRxiv - Developmental Biology 2019Quote: cDNA libraries were built using an IonTorrent S5/XL sequencer (Thermo Fisher Scientific, Waltham, MA USA). The raw reads were aligned to a horse transcriptome generated using ENSEMBL (Equ Cab 2 version ...
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bioRxiv - Developmental Biology 2019Quote: ... a cDNA library was prepared using M-MLV Reverse Transcriptase with oligo(dT) primer (ThermoFisher Scientific). Intron-spanning amplicons from pigeon PITX1 ...
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bioRxiv - Plant Biology 2021Quote: ... and cDNA libraries prepared with Superscript IV or Superscript III reverse transcriptase kits (Thermo Fisher Scientific), using oligo(dT ...
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bioRxiv - Microbiology 2020Quote: Single stranded cDNA libraries were prepared from total RNA samples using SuperScript II reverse transcriptase (ThermoFisher) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA concentrations of the final libraries were measured by Qubit dsDNA HS Assay kit (Invitrogen, Q32854). Product size distributions were visualized using Agilent’s Tapestation system (D5000 ScreenTape and Reagents ...
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bioRxiv - Microbiology 2019Quote: ... The cDNA library concentration was measured fluorometrically with Qubit dsDNA BR and HS assay kits (Invitrogen). Analysis of the RNAseq data was performed as described previously (38).
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bioRxiv - Cancer Biology 2021Quote: ... cDNA library concentration was measured using the Qubit dsDNA HS assay (Thermo Fisher, cat. no. Q32854) according to the manufacturer’s protocol and values were read on a microplate reader ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified cDNA library was amplified using AmpliTaq DNA Polymerase (Thermo Fisher Scientific, Waltham, MA, USA) and Ion 5’PCR and Ion 3’PCR primers on a Veriti 96-Well Thermal Cycler following the Ion Total RNA-Seq Kit protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... TRIzolTM RNA extraction reagent (15596018) and CloneMinerTM □cDNA library construction reagent (A11180) was purchased from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and subsequent cDNA synthesis and conversion into sequencing libraries (SOLiD Total RNA-Seq Kit, Life Technologies) was performed as per the kit protocols ...
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bioRxiv - Genetics 2021Quote: ... Two micrograms of total RNA were used to synthesize first-strand cDNA using SuperScript III First-Strand Synthesis System (Thermo Fisher Scientific). qRT-PCR analysis was then performed with gene specific primers and Applied Biosystems Power SYBR master mix (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... The resulting cDNAs were subsequently used for two-step quantitative RT-PCR with Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) on a 7500HT Fast Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Generated cDNA was diluted two to fivefold and qRT-PCR was performed using 2X Power SYBR Green PCR Master Mix (Applied Biosystems, 100029283) on a QuantStudio5 384-well PCR system ...
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bioRxiv - Neuroscience 2021Quote: The double-stranded cDNA samples were amplified by two rounds of nested PCR reactions using standard Accuprime Pfx protocols (Thermo Fisher Scientific) with 2 mins extension for each cycle ...
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bioRxiv - Immunology 2022Quote: ... Synthesized cDNA was subsequently serially diluted and subjected to two rounds of nested PCR using Platinum Taq Green Hot Start (Thermo Fisher Scientific) and primers specifically adapted for amplification of envel of HIVYU2NL4-3 (1st round primers ...
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bioRxiv - Cell Biology 2019Quote: ... Detection of RNA was performed in a two-step RT-qPCR using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, # 4374966) and the SYBR green master mix ...
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bioRxiv - Microbiology 2019Quote: RNA extracted from virus pellets (containing partially purified virus from allantoic fluid pooled from two independent experiments) was reverse transcribed (RT) using the Uni12 primer with the Verso® cDNA kit (Life Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Two micrograms of total RNA from each sample was used for first-strand cDNA synthesis using the Retroscript Kit (Applied Biosystems, ABI). The reverse transcriptase was omitted in the negative control groups for qPCR.
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bioRxiv - Microbiology 2023Quote: ... Extracted nucleic acids from VLPs and two extraction blanks were used for cDNA synthesis using the SuperScript™ IV First-Strand Synthesis System (Invitrogen™) with random hexamers provided by the manufacturer ...
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bioRxiv - Neuroscience 2020Quote: A hybrid quadrupole-orbitrap mass spectrometer (Q Exactive HF, Thermo Scientific) coupled to nano-UHPLC (Ultimate 3000 RSLC Nano ...
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bioRxiv - Molecular Biology 2022Quote: ... connected Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Scientific). The peptides were loaded on a pre-column (Acclaim PepMap 100 ...
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bioRxiv - Genomics 2022Quote: ... total RNA was extracted using a hybrid TRIzol (Life Technologies #15596026) and RNeasy Mini kit (Qiagen #74104 ...
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bioRxiv - Cell Biology 2019Quote: ... a Q Exactive hybrid quadrupole orbitrap mass spectrometer (Thermo Fisher Scientific) with a full-scan analysis over 70–800 m/z and high resolution (70,000 ...
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bioRxiv - Microbiology 2022Quote: ... coupled to an LTQ XL-Orbitrap hybrid mass spectrometer (Thermo Scientific). Peptide mixtures were injected onto the column at a flow rate of 3000 nL/min and resolved at 500 nL/min using 45 min linear gradients from 0 to 45% v/v aqueous ACN in 0.2% v/v formic acid ...
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bioRxiv - Biochemistry 2023Quote: ... coupled to a LTQ XL-Orbitrap hybrid mass spectrometer (Thermo Scientific). For nanoflow chromatography and electrospray ionization ...
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bioRxiv - Molecular Biology 2023Quote: ... connected to a hybrid LTQ-OrbiTrap XL spectrometer (Thermo Fisher Scientific) equipped with a nanoelectrospray ion source (New Objective) ...
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bioRxiv - Plant Biology 2022Quote: ... coupled to an hybrid LTQ-Orbitrap XL instrument (Thermo Fisher Scientific). The complete method has been described previously (Le Bihan et al. ...
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bioRxiv - Cell Biology 2022Quote: ... and a Q-Exactive Hybrid Quadrupole - Orbitrap mass spectrometer (ThermoFisher, USA), respectively ...