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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: Yeast two-hybrid assay was performed using the ProQuest Two-Hybrid System (Invitrogen) by PEG mediated transformation following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Yeast two-hybrid analysis was performed using the ProQuest Two-Hybrid system (Thermo Fisher). Briefly ...
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bioRxiv - Cell Biology 2021Quote: The ProQuest Two-Hybrid System (Invitrogen) was used for the yeast two-hybrid assay ...
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bioRxiv - Microbiology 2021Quote: The ProQuest Two-Hybrid System (Invitrogen) was used ...
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bioRxiv - Developmental Biology 2022Quote: Yeast two-hybrid (Y2H) interactions were characterized by employing the ProQuest Two-Hybrid System (Invitrogen Life Technologies) as reported in Cruz-Ramirez et al. ...
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bioRxiv - Developmental Biology 2022Quote: Yeast two-hybrid (Y2H) interactions were characterized by employing the ProQuest Two-Hybrid System (Invitrogen Life Technologies) as reported in Cruz-Ramirez et al. ...
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bioRxiv - Genetics 2022Quote: ... A forward Y2H library screen was performed using the ProQuest Two-Hybrid System (ThermoFisher, PQ1000101) to identify protein interactors of the C ...
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bioRxiv - Plant Biology 2023Quote: Yeast two-hybrid screening was performed according to the manufacturer’s instructions using the ProQuestTM Two-hybrid system (Invitrogen). AHL10 full length coding sequence was cloned into the entry vector pENTR-D-TOPO and recombined into the bait destination vector pDEST32 with GAL4 DNA binding domain (DBD) ...
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bioRxiv - Plant Biology 2024Quote: The yeast two-hybrid assay was performed according to previous publications using the ProQuest Two-Hybrid System (Invitrogen) following the manufacturer’s instructions52 ...
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bioRxiv - Plant Biology 2020Quote: Yeast two-hybrid assays were performed using ProQuest™ Two-Hybrid System with Gateway™ Technology (Thermo Fisher, Waltham, MA, USA). Arabidopsis ABI1 and PYR1 cDNAs cloned in pENTR-D-TOPO were introduced into pDEST22 and pDEST32 ...
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bioRxiv - Plant Biology 2019Quote: ... Directed yeast two-hybrid experiments were performed according to the ProQuest™ Two-Hybrid System with Gateway® Technology manual (Invitrogen). Full-length CMU1 and CMU2 along with a FRA1 C-terminal fragment (amino acids 616-1035 ...
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bioRxiv - Developmental Biology 2021Quote: ... Yeast two-hybrid interaction assays and color reactions were performed as described in the ProQuest Two-Hybrid System (Invitrogen #PQ10001-01, Carlsbad).
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bioRxiv - Biochemistry 2022Quote: ... Control plasmids from ProQuest™ two-hybrid system (Life Technologies). Co-transformation with empty prey vector was used as negative control ...
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bioRxiv - Plant Biology 2023Quote: Full-length wild-type and mutant cDNAs in pENTR/D-TOPO vectors were transferred to pDEST32 bait and pDEST22 prey vectors (Proquest Two-Hybrid System, Invitrogen) using Gateway LR Clonase II Enzyme mix (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... RNA was extracted from two pools of ovaries using a hybrid of Trizol (Ambion) and RNeasy mini kit (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... Gateway® cloning-compatible vectors pDEST32 and pDEST22 (Invitrogen ProQuest yeast two-hybrid System) were used ...
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bioRxiv - Molecular Biology 2021Quote: ... A cDNA library was generated using the cDNA Library Kit from Thermo Fisher Scientific (catalog A48571 ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: ... The eight rice 14-3-3 genes cloned in the yeast two-hybrid vector pDEST22 (Invitrogen) were used from a previous study (Deb et al. ...
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bioRxiv - Plant Biology 2024Quote: ... ATML1 fragments were transferred to pDEST32 bait and pDEST22 prey vectors (Proquest Two-Hybrid System, Invitrogen) using Gateway LR Clonase II Enzyme mix (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Two μg of cDNA were synthesized using a cDNA synthesis kit (Applied Biosystems) following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2020Quote: ... At1g08710 was further subcloned in yeast two hybrid vector pDEST-GBKT7 using Gateway cloning technology (Invitrogen, CA). Cloning was confirmed by colony PCR and sequencing.
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bioRxiv - Plant Biology 2021Quote: ... Plasmids used for yeast two-hybrid or Co-IP were generated by gateway LR cloning (Life Technologies) using the ENTRY vectors described above and appropriate destination vectors listed in Suppl ...
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bioRxiv - Plant Biology 2022Quote: ... and subsequently cloned into pGILDA and pB42AD yeast-two-hybrid vectors (Clonetech) via LR Gateway Reactions (ThermoFisher). To introduce T653L and Y643* mutations into ARFd1 entry clone ...
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bioRxiv - Molecular Biology 2022Quote: All plasmids used in yeast two-hybrid assays were cloned with the Gateway® Gene Cloning Technology (Invitrogen) and transformed in the AH109 and Y187 haploid yeast strains (Clontech) ...
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bioRxiv - Cell Biology 2022Quote: ... the cDNA templates were amplified from a cDNA library (Invitrogen) using gene-specific primers containing the T7 promoter sequence at each 5’ end ...
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bioRxiv - Genomics 2022Quote: ... Two strand cDNA synthesis was conducted using the high-capacity cDNA Reverse transcription kit (ThermoFisher) reverse transcriptase following manufacturer instructions ...
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bioRxiv - Genetics 2020Quote: ... we dissolved the cDNA/RNA hybrid by adding 1 µL ribonuclease H (Invitrogen 18021-014) and incubating at 37°C for 20 min.
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bioRxiv - Plant Biology 2022Quote: ... Gateway cloning was used to transfer genes for HD-Zip III and IV TFs from pENTR223.1 clones to pDEST22 and pDEST32 destination vectors (ProQuest Two-Hybrid System, Invitrogen). The bait and prey plasmids were transformed into yeast strain MaV203 (Invitrogen) ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: The wild-type copy of the xopX gene and its 14-3-3 protein binding motif mutants were cloned in the yeast two-hybrid vector pDEST32 (Invitrogen) using the Gateway cloning system (Invitrogen ...
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bioRxiv - Evolutionary Biology 2019Quote: All genes were transferred from the above generated GatewayTM compatible entry clones into the respective Yeast Two-Hybrid vectors with the GatewayTM LR Clonase II enzyme mix (Invitrogen). The codon-optimized sequences corresponding to mature proteins (without signal peptides ...
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bioRxiv - Synthetic Biology 2021Quote: All yeast strains were derived from parental strains Fy251 [American Type Culture Collection (ATCC) 96098] or the two-hybrid strain MaV20330 (Invitrogen). Yeast transformations were carried out using the lithium acetate method48 ...
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bioRxiv - Plant Biology 2022Quote: ... ATML1 cDNAs and the various deletion and missense mutations were transferred from pENTR/D-TOPO to pDEST32 bait and pDEST22 prey vectors (Proquest Two-Hybrid System, Invitrogen) using Gateway LR Clonase II Enzyme mix (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... The siRNAs used to test the role of spatacsin interactors identified by the two-hybrid screen were Silencer Select siRNAs from ThermoFisher and are listed in Supplementary Table 3.
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bioRxiv - Molecular Biology 2023Quote: ... Depletion of rRNA-DNA hybrids was achieved by two 15 min incubation periods with streptavidin-coated magnetic Dynabeads MyOne C1 (0.45 mg ThermoFisher Scientific) in 0.25 mM EDTA ...
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bioRxiv - Biochemistry 2023Quote: ... Two μg of total RNA was retro-transcribed to cDNA using the Verso cDNA Synthesis kit (ThermoFisher). RT-PCR was carried using custom-made primers (IDT technologies ...
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bioRxiv - Evolutionary Biology 2021Quote: ... cDNA libraries were synthesized using RevertAid First Strand cDNA Synthesis Kit (ThermoFisher). Specific primers for the frog ENaC α ...
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bioRxiv - Neuroscience 2024Quote: ... Two μg of total RNA was used for cDNA synthesis using High-Capacity cDNA Reverse Transcription kit (ThermoFisher). The cDNA produced was diluted 1:2 for use with LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Plant Biology 2023Quote: ... The mRNA was used create a Gateway compatible cDNA entry library by using of the CloneMiner cDNA library Construction Kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... cDNA libraries were synthesized using Invitrogen cDNA synthesis kit (Invitrogen, Grand Island, NY) and PCR was performed using SybrGreen (Roche Cat ...
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bioRxiv - Systems Biology 2022Quote: ... cDNA library was obtained by using First Strand cDNA Synthesis Kit (Thermo Fisher). For FAF2-HA and CYB5R3-HA ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA libraries were made using SuperScript-III (Invitrogen). qPCR was performed using SYBR green reagent (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA libraries were quantified via Qubit 3.0 (Invitrogen) and library quality was determined via Bioanalyzer (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... leidyi 1.2 μg mRNA were used to construct cDNA expression libraries with the Clone MinerII cDNA Library Construction Kit (Invitrogen, Waltham, USA) according to the manufacturer protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Two micrograms of total RNA were used to produce cDNA using high-capacity cDNA reverse transcription kits (Applied Biosystems). Real-time PCR was performed using the SYBR Green Master Mix System (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... The RNAs from the RNA: cDNA hybrids were eliminated using the RNase Cocktail Enzyme Mix (Thermo Fisher Scientific) in a 10-minute reaction at 37°C.
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bioRxiv - Genetics 2023Quote: ... cDNA libraries were constructed using the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems) with 10 µl of the total RNA sample ...
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bioRxiv - Genetics 2023Quote: ... cDNA libraries were constructed using the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems) with 10 µl of the total RNA sample ...
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bioRxiv - Genetics 2023Quote: ... cDNA libraries were constructed using the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems) with 10 µl of the total RNA sample ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cDNA libraries were constructed using the Collibri Stranded Library Prep Kit (Thermo Fisher Scientific) before being sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting mRNA-peptide libraries (∼1013 hybrids) were adsorbed onto 200 µL of Oligo-dT cellulose (Fisher Scientific), washed with dT washing buffer (0.2 M NaCl ...