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Citations for Thermo Fisher :
501 - 550 of 595 citations for Terlipressin Acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Electrophoretic run of samples containing 30μg of proteins was performed on precast NuPAGE 3-8% or 4-12% Tris-Acetate Protein Gels (Thermo Fisher Scientific), or on home-made acrylamide gels 8-15% ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was eluted in 50 μl water and precipitated with 0.75 M ammonium-acetate and 10 μg glycogen (ThermoFisher, Cat# R0551) by adding 125 μl ethanol (100 %) ...
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bioRxiv - Microbiology 2022Quote: ... Stool supernatant from the primary collection were obtained by centrifugation and filtration (0.2 μM surfactant-free cellulose acetate; Thermo Scientific Nalgene). Proteins were precipitated (1:7 ratio (v/v ...
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bioRxiv - Immunology 2022Quote: ... THP1 cells were differentiated into macrophage-like cells by treatment with 10nM phorbol 12-myristate 13-acetate (PMA) for a period of 72h. Lipofectamine2000 (cat no. #11668019) from Thermo Fisher Scientific or Helix-IN reagent (cat no ...
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bioRxiv - Microbiology 2023Quote: ... proteins were crashed out by addition of 300 µl ethyl acetate and all samples were evaporated to dryness in a SpeedVac vacuum concentrator (SPD131DDA SpeedVac Concentrator; Thermo Scientific). Dried samples were stored at −20C until further processing ...
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bioRxiv - Molecular Biology 2023Quote: DNA illustration by agarose gel electrophoresis has been conducted by exploiting 2% agarose gel and 1X TAE buffer (1 mM EDTA and 40 mM Tris-acetate, Fisher Scientific) and by applying constant voltage of 120 V for 40 to 60 min ...
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bioRxiv - Molecular Biology 2023Quote: ... 10μg of total protein were heat-denatured for 5 minutes at 100°C before loading onto NuPAGE 3– 8% TRIS Acetate Midi Gel (Novex, Life Technologies) and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Microbiology 2023Quote: High-performance LC grade solvents (water, acetonitrile, chloroform, and methanol) and ammonium acetate (Optima LC/MS grade) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Immunoblotting was performed according to standard procedures with the following modifications: a 3-8% tris acetate gel (Thermo Fisher Scientific, EA03752) was used and samples were run for 2 hours 15min at 80V at 4 °C in 1X tris-acetate running buffer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Cultures were stimulated with phorbol 12-myristate 13-acetate (PMA) (5 ng/mL, Alfa-Aesar) + Ionomycin (0.5 µg/mL, Thermo Fisher Scientific) and supplemented with sCTLA-4 conditioned media or murine CTLA-4-Fc fusion protein (10 µg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... all samples were buffer exchanged to 200 mM ammonium acetate with Zeba™ spin desalting columns (Thermo Fisher Scientific, Waltham, MA), with an approximate protein concentration of 2-10 µM ...
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bioRxiv - Genetics 2023Quote: ... the denatured nuclear extract was subjected to electrophoresis on a precast 3-8% Tris-Acetate polyacrylamide gel (Thermo Fisher Scientific, EA0378BOX) in 1× Tris-Acetate SDS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: Chlamydomonas reinhardtii strains were maintained on solid Tris-Acetate-Phosphate (TAP) media containing 1.6% [w/v] agar (USP grade, Thermo Fisher Scientific) and Hutner’s trace elements (64 ...
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bioRxiv - Cell Biology 2023Quote: ... stained with uranyl acetate and Sato’s lead solutions and observed in a Transmission Electron Microscope Talos L120C (FEI, Thermo Fisher Scientific) operating at 120kV ...
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bioRxiv - Biophysics 2023Quote: The samples were then buffer exchanged into either 300 mM or 500 mM ammonium acetate solution for the nucleotidylation or deRNAylation samples respectively using Zeba microspin desalting columns with a 40-kDa MWCO (Thermo Scientific). The nMS solutions contained 0.01% Tween-2045 ...
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bioRxiv - Biochemistry 2023Quote: ... stained with uranyl acetate and Sato’s lead solutions and observed in a Transmission Electron Microscope Talos L120C (FEI, Thermo Fisher Scientific) operating at 120kV.
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bioRxiv - Cell Biology 2023Quote: ... lysates were separated by sodium dodecyl sulfate/polyacrylamide (SDS/PAGE) gel electrophoresis on 1 mm NuPAGE 3 – 8% gradient Tris-Acetate gels (Thermo Fisher) using Tris-Acetate SDS running buffer and transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: The τA/3xFLAG-MYCN complex was desalted into 200 mM ammonium acetate using 75 μl Zeba spin desalting columns (ThermoFisher Scientific). Samples were analysed by nanoelectrospray ionisation MS using a quadrupole-orbitrap MS (Q-Exactive UHMR ...
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bioRxiv - Molecular Biology 2024Quote: ... extracts with high percentages of ssDNA were analyzed on denaturing gels (1 M urea, 0.8% agarose, 1x Tris-acetate-EDTA (TAE) buffer and 1x SybrGold (Thermo Fisher Scientific)) at 50 V for 3 hours ...
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bioRxiv - Microbiology 2024Quote: ... Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen) and expression was confirmed by Western blot analysis.
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bioRxiv - Genomics 2024Quote: ... Protein extracts were subjected to electrophoresis in 10% polyacrylamide SDS gels using Tris-glycine-SDS running buffer (Alaos) or in NuPAGE 3-8% Tris-acetate precast polyacrylamide gels (Thermo Fisher), with Tris-acetate SDS running buffer (Thermo Fisher) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... of the homogenized protein-loading buffer sample were loaded in duplicates on a NuPAGE™ 3-8% Tris-Acetate gel (ThermoFisher) to be probed separately with FLAG and ATPase antibodies ...
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bioRxiv - Molecular Biology 2021Quote: ... and 5 μL of input and 10 μL of eluate were separated on NuPAGE 3-8 % Tris-Acetate gels (Thermo Fisher Scientific) run at 4 °C and 35 mA/gel for 1.5 h ...
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bioRxiv - Biophysics 2019Quote: ... Purified Cas1-Cas2 complex was buffer-changed with 0.5 M Sodium acetate (pH 5.5) and labelled with EZ-Link™ Biotin-LC-Hydrazide (Thermo Scientific, Cat# 21340) and incubated overnight at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... while nine tenth of the samples were analyzed via Coomassie staining after separation on 7% NuPAGE™ Tris-Acetate protein gels (Thermo Fisher) in NuPAGE™ Tris-Acetate SDS running buffer (Thermo Fisher) ...
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bioRxiv - Bioengineering 2020Quote: ... with RPA forward and reverse primer concentrations of 400 nM and a magnesium acetate concentration of 14 mM) (25) with the following modifications: RevertAid reverse transcriptase (Thermo Fisher Scientific) and murine RNase inhibitor (NEB ...
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bioRxiv - Bioengineering 2021Quote: ... The volatile fatty acids, VFAs (acetate, propionate, and butyrate) concentrations were measured with an ion chromatography (DionexTM ICS-2100, Thermo Scientific, USA) 42 ...
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bioRxiv - Cancer Biology 2021Quote: ... A total amount of 30 µg protein was loaded per lane of NuPAGE Tris-Acetate Protein Gel 3-8% (Life Technologies, EA0375BOX) and separated according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... aliquots of 30µg of protein were prepared in native Tris glycine sample buffer and analysed from NuPAGE 3-8% Tris-Acetate polyacrylamide gel electrophoresis in a tris-glycine native running buffer (Thermo Fisher Scientific). After 2-hours of migration at 130 V ...
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bioRxiv - Cell Biology 2021Quote: ... They were then negatively stained with 2% uranyl acetate aqueous solution for 2 mins and observed under a Tecnai 12 transmission electron microscope operated at 120 kV (Thermo Fisher Scientific). The number of tubulated and non-tubulated liposomes was counted manually using Fiji (24 ...
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bioRxiv - Bioengineering 2022Quote: ... Grids were negatively stained with 1% uranyl acetate and observed in a viewed under a Tecnai 20 transmission electron microscope operating at 120 kV (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... 10 μL of sample (which is the equivalent of 10 embryos) was loaded into each lane of a 3-8% Tris-Acetate pre-cast SDS-PAGE gel (Invitrogen, Thermo Fisher Scientific) and afterwards transferred from the gel onto a nitrocellulose membrane (BIO-RAD ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μg total protein per sample was separated by SDS-PAGE using a NuPAGE™ 4-12 % Tris-Acetate gel (Invitrogen, UK) and NuPAGE™ Tris-Acetate SDS Running Buffer (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Denatured samples from both the total lysates and surface fractions were separated on NuPAGE 3-8 % Tris-Acetate protein gels (Thermo Fisher Scientific) at 200 V for 40 min ...
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bioRxiv - Microbiology 2022Quote: ... bacterial were incubated in hot phenol lysis solution (1% SDS, 2 mM EDTA, 40 mM sodium acetate in acid phenol (Invitrogen #15594-047)) for 45 minutes at 65 °C ...
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bioRxiv - Microbiology 2022Quote: ... The samples were sonicated and heated at 72°C for 10 minutes before loading 20 μl/well on NuPAGE™ 3 to 8% Tris-Acetate gels (ThermoFisher, EA03785BOX) alongside high molecular weight markers (ThermoFisher LC5699) ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were lysed in a hot (60 °C) phenol solution (2 mM EDTA, pH8, 1% SDS, 40 mM sodium acetate in acid phenol (Invitrogen #15594-047)) ...
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bioRxiv - Cell Biology 2020Quote: ... Equal masses of lysates were separated by SDS-PAGE using either 4-12% Bis Tris gels or 3-8% Tris acetate gels (Thermo Fisher Scientific). All IRS2 immunoblots were separated on 3-8% Tris acetate gels with the exception of those shown in Figures 5A and S4B ...
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bioRxiv - Genetics 2021Quote: ... The first step was to resolve the products of a standard PCR on a 3% agarose gel in Tris-acetate-EDTA gel stained with SYBR safe (Thermo Fisher Scientific), to assess the correct product size and presence of only one product ...
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bioRxiv - Genetics 2021Quote: ... SDS-Page separation was completed by running between 3 and 10μg of total protein on a NuPAGE 3%–8% Tris-acetate gel (Thermo Fisher Scientific Scientific). Next ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were then negatively stained for 1 min using 10 μl of 2% (w/v) uranyl acetate and examined with a Cryo-TEM (FEI Tecnai G2 Spirit Twin) (Thermo Fisher Scientific) using an accelerated voltage of 120 KV ...
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bioRxiv - Microbiology 2022Quote: Metabolites were extracted from cultures using both liquid-liquid extractions with ethyl acetate (EtOAc) and solid-phase extractions (SPE) using HyperSep™ C18 Cartridges (100 mg bed weight) from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Ultra-sections (70 nm) were stained with uranyl acetate and lead citrate and examined under electron microscopes Tecnai G2 Spirit (Thermo Fisher Scientific) or Hitachi HT7700 equipped with a CCD camera (Orius 832 ...
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bioRxiv - Cell Biology 2022Quote: ... the in vitro transcription reactions were quenched with Ammonium acetate stop solution from the mMESSAGE mMACHINE™ T7 Transcription Kit (Thermo Fisher) and supplemented with Phenol:Chloroform:Isoamyl Alcohol 25:24:1 (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were counterstained with uranyl acetate and lead citrate and examined in a Tecnai G2 Spirit BioTwin transmission electron microscope (Thermo Fisher Scientific) at 100 kV acceleration voltage ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were resolved due to 3-8% Tris-acetate SDS-PAGE and transferred onto methanol-activated PVDF membrane with Pierce G2 Fast Blotter (Thermo Fisher Scientific). Afterwards ...
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bioRxiv - Microbiology 2023Quote: ... 100 mM sodium acetate pH 5.5) using a 0.5 ml 7K MWCO Zeba™ Spin Desalting Column (Thermo Scientific, Cat no. 89882) and incubated at 37 degrees for 30 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... The sections were subsequently contrasted with 5% uranyl acetate and Reynold’s lead citrate and visualised with Tecnai™ G2 Spirit BioTwin transmission electron microscope (Thermo Fisher/FEI) at 80 kV with an ORIUS SC200 CCD camera and Gatan Digital Micrograph software (both from Gatan Inc.).
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bioRxiv - Immunology 2023Quote: ... The sections were subsequently contrasted with 5% uranyl acetate and Reynold’s lead citrate and visualized with Tecnai™ G2 Spirit BioTwin transmission electron microscope (Thermo Fisher/FEI) at 80kV with an ORIUS SC200 CCD camera and Gatan Digital Micrograph software (both from Gatan Inc.) ...
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bioRxiv - Molecular Biology 2023Quote: ... The synthesized sgRNAs were precipitated from the reaction using Ammonium acetate Stop Solution as described for the mMessage mMachine Kit protocol (Thermo Fisher Scientific). Cas9-mRNA was in vitro transcribed overnight at 20°C from 400-500 ng XbaI-linearized pT3TS-nCas9n (Addgene ...