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Citations for Thermo Fisher :
451 - 500 of 595 citations for Terlipressin Acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... carrying plasmids expressing DB-fused NS5 sequences were co-transformed using a standard lithium/acetate procedure with 10 ng of linearized pPC86 empty vector (Invitrogen) and 3 µL of PCR products generated from cDNA encoding TYK2 ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µL pH 7 TE was added, then 4 µL of 3 M sodium acetate (Fisher, #S210) and 0.4 µL 15 mg/mL GlycoBlue (ThermoFisher, #AM9515) or 0.3 µL 20 mg/mL RNA-grade glycogen (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... SDS-PAGE western blot was performed by loading 20 μg of each S-fraction and equal volume of the P-fraction onto precast Tris-Acetate SDS-PAGE (Novex, Invitrogen). Western blots were performed as before ...
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bioRxiv - Cell Biology 2024Quote: ... Resuspend in 50-500 µL of 1.11x tagmentation buffer (66 mM Tris-acetate (pH=7.8, BP-152, Thermo Fisher Scientific), 132 mM K-acetate (P5708 ...
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bioRxiv - Molecular Biology 2024Quote: ... boiled at 95°C for 5 mins and spun at 21 300 rcf for 5 mins prior to loading in NuPAGE Tris-Acetate gels (Invitrogen). Gels were run at 150 V for 1 h and transferred onto 0.2 µm PVDF membranes using the High MW setting on the Trans-Blot Turbo (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... 5µl samples of the PCR2 products were fractionated on a 2% agarose Tris-Acetate-EDTA (TAE) gel using Sybr Safe dye (Invitrogen) alongside a set amount of 100bp DNA ladder (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µL of ion-pairing agent (0.1% o-toluidine blue, Carolina Biological) and 3 µL of ethyl acetate (ACS grade, Fisher Scientific). The mixture was vortexed and centrifuged briefly ...
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bioRxiv - Microbiology 2023Quote: ... then 5 μg total protein in this buffer was separated by SDS-PAGE on a NuPAGE 3-8% Tris-Acetate Midi Gel (Invitrogen). Protein was transferred to a 0.45 μm PVDF Membrane (Cytiva) ...
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bioRxiv - Cell Biology 2024Quote: ... and the lysate was subjected to Western blot on NuPAGE Novex 3–8% Tris-acetate gradient gels (Invitrogen™ # EA0375BOX), transferred to a nitrocellulose membrane and blotted against OPA1 (Anti-Mouse ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were visualized by electrophoresis on 0.5% Tris-acetate-EDTA (TAE) agarose gel at 100 V for 1 h using SYBR Safe DNA Gel Stain (Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... were stained with uranyl acetate and Reynolds lead citrate and examined at 100 kV with a PHILIPS EM208S TEM (FEI - ThermoFisher) equipped with the Megaview III SIS camera (Olympus).
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bioRxiv - Microbiology 2024Quote: ... Protein extract was heat-denatured for 10 min at 80°C in NuPAGE LDS Sample Buffer an an equivalent of 2 µg total protein was separated by SDS-PAGE on a NuPAGE 4-12% Tris-Acetate Midi Gel in MOPS buffer (Invitrogen). Protein was transferred to a 0.45 µm PVDF Membrane (Cytiva) ...
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bioRxiv - Biophysics 2021Quote: ... Unreacted amine groups on the silane molecules were capped using NHS-sulfo acetate (10 mg/ml for 30 mins; Thermo Fisher). PEG-functionalized AFM cantilevers were incubated in 1 mg/ml Bovine Serum Albumin 55 overnight ...
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bioRxiv - Cell Biology 2019Quote: ... from the imaged animals were cut and stained with uranyl acetate/lead citrate (Agar Scientific, Stansted, UK) and imaged in a Tecnai T12 transmission electron microscope (Thermo Fisher) operating at 100 KV equipped with a digital camera (Veleta) ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples of SMG6-3xFLAG in vitro translation reactions were run on 3-8% Tris-Acetate NuPAGE gel (Invitrogen, Cat. No EA03752BOX). Transfer onto nitrocellulose membranes was performed using the iBlot 2 Dry Blotting System (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... For MS of permethylated glycolipids in positive ion mode,∼0.4 nmol of permethylated total glycolipids were dissolved in 50 µl of 1 mM sodium acetate in methanol/water (1:1) for infusion into a linear ion trap mass spectrometer (Orbi-LTQ; ThermoFisher Scientific) using a nanospray source at a syringe flow rate of 0.40 µl/min and capillary temperature set to 210 °C [18–21] ...
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bioRxiv - Immunology 2021Quote: ... and checked for purity and quality on a silver-stained SDS-PAGE gel (3-8% Tris-Acetate Novex, Thermo Fisher Scientific). The anti-NTD neutralizing antibody NTD-18 and the pan-coronavirus anti-S2 non-neutralizing antibody Ab-10 were previously described 9,10.
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bioRxiv - Cancer Biology 2019Quote: ... LDHA proteins in tumor extract were separated on 4% to 20% Tris-Glycine gel and CD31 was separated on NuPAGE 3 to 8 % Tris-Acetate gel (Life Technologies) by SDS-PAGE and were transferred to nitrocellulose membrane ...
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bioRxiv - Genomics 2020Quote: ... 1% Triton X-100 and 10% glycerol and resolved on 3–8% pre-cast gradient Tris-Acetate gels (Invitrogen, Waltham, MA). For mouse samples ...
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bioRxiv - Microbiology 2019Quote: ... 100 µL of culture supernatants or BALF were extracted twice with 100 µL of ethyl acetate and concentrated to 20 µL in a 60 Hz Savant SpeedVac DNA 100 concentrator (ThermoFisher Scientific). 1 µL of culture supernatants or 7.5 µL of BALF samples were spotted onto aluminum-backed C18-W silica plates (Sorbent Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... proteins were resolved for 4 hr at 100 V on 4% −8% polyacrylamide gradient Tris-acetate denaturing gels (Novex, Life Technologies) and transferred to PVDF membranes ...
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bioRxiv - Biophysics 2021Quote: ... according to the manufacturer’s protocol and then purified using one round of each phenol/chloroform and chloroform extraction followed by precipitation using ethanol in presence of 300 mM sodium acetate pH 5.5 and GlycoBlue coprecipitant (Thermo Fisher Scientific). RNA concentration and purity were determined by absorbance measurement ...
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bioRxiv - Biochemistry 2020Quote: ... 10 μg protein/lane was then separated under denaturing conditions on reducing 3~8% Tris-Acetate gels (NuPAGE, Thermo Fisher Scientific), and HiMark protein standard (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were denatured by adding 4xLDS buffer and heating at 70°C for 10 min before being separated in 3-8% Tris-acetate gels (Thermo Fisher) run at 150 V for 3 hours ...
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bioRxiv - Bioengineering 2021Quote: ... 1.6 μm particle size) at the flow rate of 150 μl min-1 under a gradient of ammonium acetate and acetonitrile (Fisher Scientific). Multiple reaction monitoring (MRM ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were then resolved on 4-12% Bis-Tris gels (for lower molecular weight proteins) or 3-8% Tris-Acetate gels (for higher molecular weight proteins) (NuPage, Thermo-Fisher Scientific) and run in MOPS or Tris-Acetate running buffer respectively ...
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bioRxiv - Bioengineering 2020Quote: ... Cisapride and norcisapride were further separated by a gradient mode of 10 mM ammonium acetate-acetonitrile as mobile phase using Acclaim Trinity P1 reverse phase column (2.1 × 50mm, 3 μm; Thermo Fisher Scientific). Mass ion transitions (Q1/Q3 ...
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bioRxiv - Cell Biology 2020Quote: ... were loaded on to NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate 1.0 mm Mini Protein Gels (Invitrogen, NP0321, EA0375PK2) buffer alongside PageRuler Protein Ladder (5 µL/lane ...
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bioRxiv - Molecular Biology 2022Quote: Protein extracts were resolved using either home-made SDS-PAGE gels or 3-8% NuPAGE Tris-Acetate gels (Thermo Fisher Scientific), when analysing proteins of a molecular weight >180 kDa ...
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bioRxiv - Microbiology 2022Quote: ... stained by uranyl acetate 2% in H2O for 10 min and examined at 100kV by EM 208S Transmission Electron Microscope (FEI - Thermo Fisher) equipped with the acquisition system Megaview SIS camera (Olympus).
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bioRxiv - Pathology 2022Quote: ... Protein concentration was determined with Bio-Rad Protein Assay Kit II and 15 μg sample protein was loaded in a NuPAGE 3-8 % Tris-Acetate gel (Thermo Fisher). The gel was run at a constant voltage of 150 V for 4 h ...
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bioRxiv - Biophysics 2022Quote: ... Purified surface proteins or total cell lysates were separated on a NuPage 3-8 % Tris-acetate protein gel (Thermo Fisher Scientific) at 200 V for 40 min and transferred to a PVDF membrane ...
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bioRxiv - Biochemistry 2022Quote: ... All ssDNAs were dialyzed into 100 mM ammonium acetate with Pierce 96-well microdialysis devices with 3.5K MWCO (Thermo Fisher Scientific). For preparation of LiRecT-DNA complexes ...
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bioRxiv - Immunology 2022Quote: ... The quality/purity of the different purified fractions was evaluated by SDS-PAGE using 3–8% Tris– Acetate gels (Life Technologies) under non-reducing conditions followed by silver staining (Silver Stain kit ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated at 95°C for 5 min and the supernatant loaded onto a either 3-8% Tris-acetate or 4-12% Bis-Tris gradient gels (Life Technologies).
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bioRxiv - Microbiology 2019Quote: ... post-stained in saturated Uranyl acetate in 70% Ethanol (Roth) for 7 minutes and subsequently imaged using an FEI Tecnai F20 electron microscope (Thermofisher, USA).
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bioRxiv - Cell Biology 2021Quote: ... Protein lysates were separated on tris-glycine or tris-acetate SDS-PAGE gels and transferred to a nitrocellulose membrane using the iBlot 2 dry blotting system (Invitrogen IB21001). The following primary antibodies where used for western-blotting at 1/1000 dilution ...
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bioRxiv - Systems Biology 2021Quote: ... The final transformation mixture contained 10 mM LiAc (Lithium acetate dihydrate, 98%, extra pure, ACROS Organics™, CAS 6108-17-4), 50 µg ssDNA (UltraPure™ Salmon Sperm DNA Solution ...
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bioRxiv - Genetics 2019Quote: ... 40-50μg of protein was analysed by SDS–polyacrylamide gel electrophoresis (SDS–PAGE) using 3-8% NuPAGE Tris-Acetate protein gels (Life Technologies) and transferred onto nitrocellulose membrane using Xcell Sure Lock Mini Cell electrophoresis tanks (Novex ...
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bioRxiv - Genomics 2021Quote: ... Purified DNA was eluted with 4 μL of 10 mM Tris-acetate (pH 8.0) and analyzed on a 10% Novex TBE-Urea gel (Thermo Fisher Scientific). Fluorescent images were obtained using Typhoon Trio+ and analyzed with ImageQuant software (GE Healthcare).
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bioRxiv - Physiology 2021Quote: ... Proteins were separated on Bolt™ 4-12% Bis-Tris Plus gels or 7% Tris-Acetate gels (Invitrogen, Thermo Fisher Scientific), blotted onto a nitrocellulose membrane (926-31092 ...
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bioRxiv - Physiology 2021Quote: ... Proteins were separated on Bolt™ 4-12% Bis-Tris Plus gels or 7% Tris-Acetate gels (Invitrogen, Thermo Fisher Scientific), blotted onto a nitrocellulose membrane (926-31092 ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were passed through a 25G syringe five times to reduce viscosity and separated on a 3%-8% NuPAGE Tris-Acetate gel (Thermo Fisher). Proteins were transferred onto a polyvinylidene fluoride membrane (Thermo Scientific ...
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bioRxiv - Genomics 2020Quote: ... An estimated 60 µg of protein was loaded per well on a NuPAGE Novex 3-8 % Tris-Acetate Protein gel (Life Technologies), separated by electrophoresis and blotted on polyvinylidene fluoride membranes ...
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bioRxiv - Developmental Biology 2020Quote: ... For the detection of Gli3 10μg protein lysates were subjected to gel electrophoresis on a 3-8% NuPAGE® Tris-Acetate gel (Life Technologies), and protein was transferred to a Immobilon-FL membrane (Millipore) ...
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bioRxiv - Cell Biology 2021Quote: ... The grid was stained with 2% uranyl acetate and air-dried before loading to a Talos L120C microscope (Thermo Fisher Scientific). The length of the array was directly measured in the ImageJ and converted to the equivalent number of OADs for subsequent quantitative analysis.
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bioRxiv - Immunology 2022Quote: ... Cells were then loaded with CellTrace® cell dye (CFSE and Violet, for control and acetate treatment) according to manufacturer’s instructions (Thermo Fisher) and incubated further 30 minutes with corresponding treatment (control or acetate containing R10 medium) ...
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bioRxiv - Cell Biology 2022Quote: ... stained with uranyl acetate and Sato’s lead solutions and observed in a Transmission Electron Microscope Talos L120C (FEI, Thermo Fisher Scientific) operating at 120 kV ...
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bioRxiv - Immunology 2022Quote: ... the B cells from these wells were transferred to microcentrifuge tubes and washed three times with BTX medium (300 mM sorbitol [Fisher Scientific, cat. # BP439], 0.1 mM calcium acetate [Fisher Scientific, cat ...
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bioRxiv - Microbiology 2022Quote: ... contrast stained for 10 min with 2% uranyl acetate and visualized in a TEM (L120C, Talos, Thermo Fisher Scientific, MA, USA).