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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for TNF alpha Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Isolated rat bone marrow MSCs were cultured in alpha MEM (ThermoFisher Scientific, Burlington ON, Canada) with 10% fetal bovine serum (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... washed 2x with PBS and changed to serum free alpha-MEM (Invitrogen, Waltham, MA, USA) with 1% pencilin-streptomycin ...
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bioRxiv - Physiology 2023Quote: Balb/c mice were injected subcutaneously (s.c.) with 40IU of EPO (Recombinant HuEpo-alpha, Gibco) for four consecutive days ...
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bioRxiv - Neuroscience 2022Quote: ... MSCs were cultured in alpha-modified Eagle’s medium (α-MEM, Gibco Invitrogen, Carlsbad, CA, USA) supplemented with 1% L-Glutamine (Macopharma) ...
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bioRxiv - Neuroscience 2022Quote: ... MSCs were cultured in alpha-modified Eagle’s medium (α-MEM, Gibco Invitrogen, Carlsbad, CA, USA) supplemented with 1% L-Glutamine (Macopharma) ...
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bioRxiv - Plant Biology 2023Quote: ... 0.03% (w/v) L-glutamine (Alpha Caesar #A14201) 2% (w/v) sucrose (Fisher Scientific #10634932), and 100 μM acetosyringone ...
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bioRxiv - Neuroscience 2023Quote: ... samples were incubated with alpha-bungarotoxin conjugated with Alexa594 (α-btx594, 4h; Thermo Fisher Scientific) to stain the acetylcholine receptors (AChRs) ...
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bioRxiv - Cancer Biology 2022Quote: ... with the addition of Super Bright 600 anti-CD49f (integrin alpha 6) antibody (Thermo Scientific, 63-0495-42 ...
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bioRxiv - Developmental Biology 2023Quote: ... and resuspended in Alpha Minimum Essential Medium (α-MEM; GIBCO BRL, Grand Island, NY, USA) supplemented with 15% (v/v ...
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bioRxiv - Bioengineering 2019Quote: ... The resulting VH-(G4S)3-VL ScFv fragment was further fused at the N-terminus of the murine TNF gene through a S4G-linker and the final construct VH-(G4S)3-VL-(S4G)3-TNF was then cloned into the mammalian expression vector pcDNA3.1 (+) vector (Invitrogen). A VH-(G4S)3-VL ScFv fragment specific for hen egg lysozyme (KSF)(34) ...
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bioRxiv - Molecular Biology 2023Quote: ... TNF-α and IL-6 in cell culture supernatants were assayed using standard ELISA kits (Invitrogen or R&D) in accordance with the protocol set out by the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... and TNF-α (cat#BMS223-4) were measured in duplicate using human ELISA kits following the manufacturer’s instructions (ThermoFisher) and SpectraMax M3 (Molecular devices).
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bioRxiv - Cell Biology 2024Quote: ... this is equivalent to total of 190,000 cells) in the morning and stimulated overnight with 10 ng/mL TNF (PHC3015, Invitrogen). The RBC control was prepared by culturing a 2% haematocrit suspension in RPMI medium overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... chicken TNF-α (LITAF, assay ID number Gg03364359_m1) and the reference gene chicken GAPDH (assay ID number Gg03346982_m1) (Thermofisher Scientific). The PCR cycle parameters were an initial denaturing step at 95 °C for 10 min followed by 45 cycles of 95 °C for 15 s and 60 °C for 1 min ...
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bioRxiv - Immunology 2019Quote: Cells were stimulated with 100ng/mL of TNC-TNF for 2h and RNA was extracted using GENEzol Reagent (Geneaid) and cleaned using PureLink RNA Mini Kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... TNF-α and IL-6 in culture supernatant was quantified by commercial ELISA kits (Thermo Fisher Scientific, Waltham, MA, USA). The measurement of OD (Optical density ...
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bioRxiv - Immunology 2022Quote: ... Neutrophils were then stimulated with 50-100 ng/mL tumor necrosis factor (TNF; Miltenyi) or 100 μg/mL of the Dectin-1 ligand Curdlan (Wako biochemical) in flatbottom plates (Nunc) at 37°C ...
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bioRxiv - Microbiology 2024Quote: TNF-α concentration in cell culture supernatants was determined using Ready-SET-Go! ELISA kits (ThermoFisher, Cat. 88-7324-88) according to the manufacturer’s instruction ...
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bioRxiv - Biophysics 2022Quote: HeLa (ECACC 93021013) and MCF-7 (ECACC 86012803) cells were cultured in MEM Alpha medium (Gibco), with GlutaMax (no nucleosides) ...
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bioRxiv - Neuroscience 2019Quote: ... samples were incubated in Alexa594-alpha-bungarotoxin (Invitrogen, B-13423, 50μg/ml, 1:500 in PBS). Tissue was washed 3x in PBS and mounted on a slide with Vectashield mounting media ...
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bioRxiv - Immunology 2022Quote: ... The cells were then resuspended with the same volume of pre-warmed MEM-alpha (Gibco, 12561) + 10% HI-FBS (Gibco ...
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bioRxiv - Immunology 2021Quote: The cloning reactions were used to transform OneShot DH5 Alpha cells (Thermo Fisher Scientific, Cat#: 12297016) according to manufacturer’s directions and plated on agar plates containing ampicillin and grown overnight ...
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bioRxiv - Neuroscience 2021Quote: ... the inoculum was removed and cells were overlaid with semisolid medium constituted of alpha-MEM (Gibco) containing 1.25% carboxymethyl cellulose (Sigma Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... The NK- 92 MI (ATCC CRL-2408) cell line was grown in Alpha-MEM medium (Gibco) with 2mM L-Glutamine ...
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bioRxiv - Molecular Biology 2022Quote: ... NHDF (WT and PML KO cells) were cultured in alpha-modified Minimum Essential Medium (Life Technologies) supplemented with 15% fetal calf serum and GlutaMAX (Thermo Fisher ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Human MSCs were cultured in alpha Modification of Eagle’s minimal essential medium (αMEM) (Thermo Fisher Scientific) supplemented with 15% FBS (Gemini Bio-Products) ...
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bioRxiv - Cell Biology 2023Quote: ... Wild-type HeLa cells (ATCC) and stable HeLa cell lines were maintained in MEM-alpha (Gibco), supplemented with 10% v/v fetal bovine serum at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Bioengineering 2023Quote: ... The osteochondral explants were cultured overnight in alpha-Minimum Essential Medium (α-MEM; Gibco, Massachusetts, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... alpha-MEM supplemented with 10% Fetal Bovine Serum (FBS) and 1% penicillin/streptomycin (p./s.) (Gibco). All cultures were maintained in a 37□ incubator with 5% CO2.
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bioRxiv - Developmental Biology 2024Quote: ... Denuded oocytes were maintained at the GV stage in alpha-minimal essential medium (α-MEM) (Gibco) supplemented with 0.2 mM sodium pyruvate ...
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bioRxiv - Immunology 2021Quote: ... 17-7311-81) and TNF-α (eBioscience, 12-7321-81)) were stained before fixation/permeabi-lization (Intracellular Fixation & Permeabilization Buffer Set, ThermoFisher).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... IL-6 and TNF-α in RAW264.7 cells culture supernatants were analyzed using ELISA kits (No. EMIL6RA, 88-7324-22, bms6002; Thermo Scientific) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... ELISAs were conducted following the manufacturers’ protocols, with the IL-1b and IFN-y (ELISAKIT.com, EK-0033 and EK-0002, respectively) and TNF-a (ThermoFisher Scientific KMC4022). Samples were diluted 1 in 5 and 1 in 10 for IL-1b ...
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bioRxiv - Immunology 2020Quote: ... Capture antibodies were anti-IL-6 (14-7061-85, eBioscience), anti-TNF-α (14-7423-85, eBioscience) and anti-IL-1β (14-7012-81, Invitrogen). Detection antibodies conjugated with biotin were anti-mouse-IL-6 (554402 ...
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bioRxiv - Cell Biology 2022Quote: ... established mature DB-VOs at day 15 from diabetic iPSCs were started to culture in high glucose concentration (33mM, adapted from our previous publication (Yang et al., 2020)) plus 1 ng/ml human TNF (Invitrogen, PHC3011) and 1 ng/ml IL-6 (Invitrogen ...
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bioRxiv - Synthetic Biology 2023Quote: ... Samples that were determined to be below the detection limit where quantified using <0.3 pg/mL sensitivity porcine TNF-α ELISA kit (Invitrogen, USA).
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bioRxiv - Bioengineering 2023Quote: ... MSCs were treated with either MSC-GM or MSC-GM + 50 ng/mL IFN-γ/TNF-α (Gibco, Sino Biological, respectively) for 24 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were plated at a density of 5×104 mononuclear cells per cm2 in alpha MEM (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Physiology 2019Quote: ... Each ovary was halved and incubated in collagenase media (Minimum essential medium alpha (αMEM, Gibco, 12561-056), 0.5% PenStrep ...
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bioRxiv - Bioengineering 2020Quote: ... MSCs were rinsed twice with PBS before adding fresh serum free medium (Alpha-Minimum Essential Medium (Gibco), 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody dilutions (and suppliers) are as follows: alpha-tubulin DM1A 1:5000 (Thermo Fisher Scientific, Cat# MS581P1); TCF11/NRF1/NFE2L1 D5B10 1:1000 (Cell Signaling Technology ...
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bioRxiv - Bioengineering 2020Quote: ... Fifty microliter clots were formed from purified fibrinogen using 0.25 U/mL human alpha-thrombin (Fisher Scientific), 2 mg/mL fibrinogen (Enzyme Research Laboratories) ...
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bioRxiv - Bioengineering 2021Quote: ... The alpha-tubulin goat antimouse antibody was revealed with Alexa Fluor 488 goat antimouse (Invitrogen, ThermoFisher Scientific). We rinsed Hela cells three times in PBS for 15 min ...
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bioRxiv - Bioengineering 2021Quote: ... The alpha-tubulin goat antimouse antibody was revealed with Alexa Fluor 488 goat antimouse (Invitrogen, ThermoFisher Scientific). We rinsed Hela cells three times in PBS for 15 min ...
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bioRxiv - Biochemistry 2022Quote: ... MSCs were cultured in a standard medium composed of MEM alpha (Thermo Fisher Scientific, Waltham, MA, USA), 0.5% gentamycin (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... MCF7 cells were maintained in Minimum Essential Medium (MEM) Alpha no phenol red (Life Technologies, Carlsbad, CA) containing 10 % FBS ...
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bioRxiv - Developmental Biology 2023Quote: ... The OP9-DLL1/MS5-DLL4 cell lines were maintained in Alpha Minimum Essential Medium (Thermo Fisher, 12000063) with 2.2 g/L sodium bicarbonate (Corning ...
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bioRxiv - Cancer Biology 2024Quote: ... Fetal liver cells were thawed and cultured in alpha-minimum essential medium (α-MEM) GlutaMAX (Gibco, 35050061) supplemented with 10% FCS (Gibco) ...
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bioRxiv - Developmental Biology 2023Quote: ... The media was composed of bicarbonate-buffered minimal essential medium alpha (MEMα; Thermo Fisher Scientific, Gaithersburg, MD) supplemented with 3 mg/ml of bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... The blood samples were then centrifuged at 2000 xg for 10 minutes and serum collected for TNF determination by ELISA (Invitrogen, Carlsbad, CA) following the manufacturer’s instructions.