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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for TNF alpha Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: TNF-α was quantified in cell culture supernatants of infected cells by Ready-SET-Go! ELISA kits (Thermo Fisher Scientific, Cat. 88-7324-77) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... The attached cells were cultured for 16 days with alpha minimum essential medium (α-MEM, Gibco BRL, Gaithersburg, MD, USA) supplemented with 20% FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... and primary fibroblasts obtained from lungs of healthy NSG or BALB/c mice 6-8 weeks of age were cultured in Minimum Essential Medium Eagle medium with alpha modification (MEMα) (ThermoFisher) supplemented with 1x MEM Non-essential Amino Acid Solution (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... The NK-92 cells were obtained from DSMZ (ACC 488) and grown in in MEM Alpha medium (Gibco, #22571-020) supplemented with 5 ng/mL recombinant human interleukin 2 (Gibco ...
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bioRxiv - Bioengineering 2021Quote: ... MSCs were thawed and allowed to recover for 24 hours in complete medium (MSC-GM) (Alpha-Minimum Essential Medium (Gibco), 10% fetal bovine serum (Hyclone) ...
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bioRxiv - Physiology 2021Quote: Ocy454 cells were maintained in alpha-minimum essential medium (MEM) supplemented with heat-inactivated 10% fetal bovine serum and 1% antibiotic–antimycotic (Gibco) at 33 °C with 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained (10 μL/1.5×105 cells) with anti-IL-1 alpha (Thermo Fisher Scientific; Cat.# 11-7118-81) and anti IgG1 kappa (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Primary bone marrow monocytes from isolated from the tibiae by flushing the marrow cavity with osteoclast media (phenol red-free alpha-MEM (Gibco) with 25 units/mL penicillin/streptomycin (Invitrogen) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Bone marrow was collected from the femoral cavity of 7-day-old SD rats by flushing of medium using a 22-gauge needle and cultured in complete growth medium (alpha-modified eagle’s medium (αMEM, GIBCO, USA), 10% fetal bovine serum (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... MSCs from bone marrow Ewing patients were isolated by density-gradient centrifugation using Ficoll technique and were cultured in alpha MEM (Gibco), MSC-qualified serum (Gibco) ...
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bioRxiv - Cell Biology 2019Quote: ... BM was flushed from cut femurs and tibiae using 3-ml syringes with Minimum Essential Medium Alpha (αMEM without ribonucleosides or deoxyribonucleosides; Gibco, Life Technologies cat ...
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bioRxiv - Cell Biology 2021Quote: ... Cellmatrix Type I-C) fibril-coated plate (defined as passage 0, population doubling level 0) and cultured in expansion medium (alpha-MEM (Invitrogen), 10% FBS (Hyclone) ...
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bioRxiv - Bioengineering 2022Quote: ... hMSCs were grown in alpha-minimum essential media (1×) (supplemented with L-glutamine, ribonucleosides, and deoxyribonucleosides) (Gibco, Cat. #12561-056) containing 20% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2022Quote: Human breast cancer (MCF-7) cells were obtained from ATCC and cultured every two days in Minimum Essential Medium (MEM) Alpha media (Gibco) supplemented with 1 mM sodium pyruvate ...
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bioRxiv - Molecular Biology 2023Quote: ... Chemical composition of the sample surfaces was analyzed by X-ray photoelectron spectroscopy (XPS, Thermo Fisher Scientific K-Alpha XPS) with a 400 μm microfocused monochormatic Al Kα X-ray source ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated in cardiac troponin T antibody (MS-295-P1, Epredia) diluted 1:400 in PBST or alpha-actinin antibody (MA1-22863, Invitrogen) diluted 1:200 in PBST overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: Alexa 647 labelled Goat anti-Rabbit IgG secondary antibody and anti-alpha tubulin primary antibody was purchased from Thermo Fisher Scientific (Australia) ...
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bioRxiv - Developmental Biology 2023Quote: ... the cell culture medium was changed into myogenic differentiation medium composed of alpha-MEM (Nacalai tesque) with 5% of KSR (GIBCO) and 500ng/ml of Dox ...
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bioRxiv - Cancer Biology 2019Quote: ... tumor necrosis factor α (TNF-α) and TATA box binding protein (TBP) were determined by real☐time PCR (ABI 7900 Prism, Applied Biosystems, US). Sequences used to amplify a fragment of IL-6 were FP ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stimulated using a half media change with a final cytokine cocktail of 5 ng/mL IFN-ү/TNF-α (Gibco, Sino Biological, respectively). MSC-EV treatment groups received an additional 10 uL of MSC-EV preparation for the 1X dilution ...
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bioRxiv - Physiology 2021Quote: ... mouse anti-mouse GAPDH (Thermo Fisher, #AM4300), rabbit-anti-mouse LSD1 (Abcam ...
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bioRxiv - Biophysics 2021Quote: ... AML cells were grown in suspension in twelve flasks containing 150 mL alpha minimum essential medium (Invitrogen Canada Inc., Burlington, Canada), with 5% fetal bovine serum (Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Murine dendritic cell line JAWSII was purchased from ATCC and was cultured in alpha minimum essential medium with 20% FBS (Life Technologies), ribonucleosides ...
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bioRxiv - Developmental Biology 2021Quote: Protein expression analysis by WB was performed as described (Grivas et al., 2020), using antibodies Mdka (Calinescu et al., 2009) and Alpha-Tubulin (Thermofisher scientific).
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bioRxiv - Microbiology 2019Quote: Mtb was grown as described above and processed for ChIP experiments using purified anti-PhoP (Alpha Omega Sciences) or anti-FLAG (Thermo Scientific) antibody and protein A/G agarose beads (Pierce ...
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DNA uptake by cell wall-deficient bacteria reveals a putative ancient macromolecule uptake mechanismbioRxiv - Microbiology 2022Quote: ... cells of alpha pKR2 were incubated with a final concentration of 1 mg ml-1 Dextran-Texas Red (3000 MW, neutral, Molecular Probes) in PBS and were imaged overnight ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA samples were prepared from freeze-dried cells (CHRIST Alpha 1‐2 LDplus lyophilizer, Osterode, Germany) derived from untreated and farnesol-treated cultures using TRISOL (Invitrogen, Austria) reagent ...
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bioRxiv - Molecular Biology 2019Quote: ... The OCI-LY19 cell line (DSMZ, ACC 528) was cultured in Minimum Essential Medium Eagle - Alpha Modification (Gibco, catalog number 12000063) supplemented with 2.2g/L of sodium bicarbonate (Thermofisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: The murine preosteoblast cell line MC3T3-E1 clone 14 was purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and cultured in alpha modified Eagle’s medium (α-MEM, Gibco, USA) supplemented with 10% FBS (HyClone ...
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bioRxiv - Immunology 2021Quote: ... Murine dendritic cell line JAWSII was purchased from ATCC and was cultured in alpha minimum essential medium with 20% FBS (Life Technologies), ribonucleosides ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor 488-Phalloidin diluted 1:150 to visualize F-actin and rabbit anti-alpha-actinin-1 (Thermo Fisher Scientific, USA) diluted 1:25 ...
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bioRxiv - Biochemistry 2020Quote: ... cells were incubated with an alpha tubulin monoclonal antibody conjugated to Alexa Fluor 488 (B-5-1-2, Invitrogen, Carlsbad, CA) at 2 μg/mL in 1% BSA in PBS for 1 hour ...
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bioRxiv - Physiology 2022Quote: ... Inflammatory OCLs were differentiated by seeding a total of 2×104 CD11c+ DCs/well on 24-well plates in MEM-alpha (ThermoFisher Scientific) including 5% serum (Hyclone ...
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bioRxiv - Neuroscience 2023Quote: ... and 62.5 ng of cDNA was amplified using TaqMan probes for vascular endothelial growth factor alpha (Vegfa) and 18S as endogenous control (Life Technologies), and SYBR green probes for cd93 ...
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bioRxiv - Physiology 2023Quote: ... Samples were washed three times with PBST and incubated with Alex-Fluor 488 conjugated alpha-bungarotoxin (1:500 dilution, B13422, Thermo Fisher) and Cy-3 conjugated anti rabbit IgG at (dilution 1:300 ...
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bioRxiv - Developmental Biology 2023Quote: IVM for the GV oocytes was conducted by washing the oocytes twice and culturing in alpha-modified Eagle’s minimum essential medium (Thermo Fisher Scientific) containing 5% (vol/vol ...
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bioRxiv - Cell Biology 2024Quote: ... were cultured in alpha minimum essential media (αMEM; Biological Industries, 01-042-1A) supplemented with 10% fetal calf serum (FBS; Invitrogen, 10270106), 100 IU/mL penicillin ...
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bioRxiv - Physiology 2023Quote: ... and human tumor necrosis factor-α (TNF-α) used in the incubation of pancreatic β cells and isolated islets were purchased from Invitrogen (Carlsbad, CA, USA). Ca2+ imaging of mouse islets was performed with Fura-2 AM (Fura-2-acetoxymethyl ester ...
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bioRxiv - Microbiology 2019Quote: ... and cultured in Minimum Essential Medium Alpha Modification (MEM-α, HyClone) with 20% fetal bovine serum (FBS, HyClone) and 1% penicillin-streptomycin (P/S, Gibco Life Technologies). Goat adipose-derived mesenchymal stem cells (gASCs ...
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bioRxiv - Bioengineering 2021Quote: hiPSC-derived SSCs (hSSCs) were differentiated as previously described.[30] hiPSCs were grown on Matrigel in Minimum Essential Medium Alpha (αMEM, Gibco A10490-01), 1X GlutaMAX ...
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bioRxiv - Cell Biology 2021Quote: ... Mononuclear cells were collected by Ficoll-based density gradient centrifugation and cultured in T75 flasks at a density of 160,000/cm2 in MEM alpha basic (C12571500BT MEM α, Nucleosides MEM, Invitrogen, Carlsbad, CA) culture medium supplemented with penicillin and streptomycin (Gibco ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: Human wild-type (WT) alpha-synuclein was expressed in Escherichia coli One Shot® BL21 STAR™ (DE3) (Invitrogen, Thermo Fisher Scientific) cells using plasmid pT7-7 and purified using ion-exchange on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: Human wild-type (WT) alpha-synuclein was expressed in Escherichia coli One Shot® BL21 STAR™ (DE3) (Invitrogen, Thermo Fisher Scientific) cells using plasmid pT7-7 and purified using ion-exchange on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... Alpha-minimum essential medium (α-mem) supplemented with platelet lysate 10% and B27 1/100 (all from Life Technologies, Carlsbad, CA, USA). A ROCK inhibitor (Y27632 10 ng/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Embryo vitrification and warming were carried out in Handling Medium (HM; alpha Minimal Essential Medium; αMEM; Gibco by Life Technologies, CA, USA) containing ...
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bioRxiv - Cell Biology 2020Quote: ... Embryo vitrification and warming were carried out in Handling Medium (HM; alpha Minimal Essential Medium; αMEM; Gibco by Life Technologies, CA, USA) containing ...
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bioRxiv - Bioengineering 2022Quote: ... cluster of differentiation 31 (CD31, rat IgG2a) primary antibody and alpha-smooth muscle actin (α-SMA, rabbit IgG) primary antibody were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2022Quote: ... The mutations for Alpha and Delta were added in silico to the codon-optimized Wuhan strain and ordered as synthetic genes (GeneArt, Thermo Fisher Scientific) and cloned into the same backbone ...
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bioRxiv - Bioengineering 2022Quote: ... Human bone marrow mesenchymal stem cells (hMSCs, RoosterBio, Maryland, USA) were cultured in alpha Minimal Eagle’s medium (α-MEM) (Gibco, Carlsbad, USA) with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated in 500 μL of primary antibody solution (rat anti-alpha Tubulin YL1/2 (MA1-80017, Invitrogen, CA, SAD), diluted 1:500 ...