Labshake search
Citations for Thermo Fisher :
251 - 300 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2019Quote: Human embryonic kidney (HEK) 293T cells were cultured in DMEM (GIBCO 11995065), 10% FBS (Gibco ILT10082147) ...
-
bioRxiv - Microbiology 2019Quote: ... we grew human embryonic kidney (HEK) 293FT cells (Thermo Fisher Scientific; #R70007) in Dulbecco’s modified Eagle medium (DMEM ...
-
bioRxiv - Cell Biology 2023Quote: ... Pooled neonatal primary Human Epidermal Keratinocytes (HEK) were obtained from Life Technologies and were used between passages two to five ...
-
bioRxiv - Bioengineering 2020Quote: ... HEK 293T cells were cultured in DMEM (HyClone) supplemented with 10% heat-inactivated FBS (HI-FBS; ThermoFisher). CHLA-255-Luc-EGFP cells were cultured in IMDM (ThermoFisher ...
-
bioRxiv - Neuroscience 2023Quote: ... Human fibroblasts and Human embryonic kidney (HEK) 293T cells were cultured in DMEM (1g/L glucose) (GIBCO) supplemented with 10% Fetal Calf Serum (FCS) ...
-
bioRxiv - Cancer Biology 2021Quote: ... was then gently mixed with 20 parts (v/v) of HEK-293 cells (ATCC) suspended at a density of 2 x 105 cells/mL in DMEM (Gibco) + 10% FBS (Seradigm/VWR) ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... HEK-293 cells were transferred with a density of 12000 cells per well in white 96-well plates (Thermo Scientific™ Nunc™ F96 MicroWell™ ...
-
bioRxiv - Biochemistry 2020Quote: ... HEK 293T and T-Rex 293 cells were grown in Dulbecco’s modified Eagles medium (DMEM, Thermo Fisher scientific, MA, USA) containing 4.5 mg/ml glucose ...
-
bioRxiv - Neuroscience 2020Quote: ... Lentiviruses were produced by transfecting HEK 293 cells with lentiviral vectors together with packaging vectors (3rd generation) using Lipofectamine 3000 (Thermofisher). Viral supernatant was collected 24hr and 48hr post-transfection ...
-
bioRxiv - Genetics 2022Quote: HEK 293 cells were cultured in medium consisting of high glucose DMEM (Hyclone) supplemented with 10% fetal bovine serum (Gibco) and 1× penicillin-streptomycin (Gibco) ...
-
bioRxiv - Microbiology 2020Quote: ... The pSAd36-S and pSAd-control plasmids were linearized with PacI restriction enzyme to liberate viral genomes for transfection into T-Rex 293-HEK cells (Invitrogen). The rescued replication-incompetent ChAd-SARS-CoV-2-S and ChAd-Control vectors were scaled up in 293 cells and purified by CsCl density-gradient ultracentrifugation ...
-
bioRxiv - Microbiology 2022Quote: The viability of HEK-293 cells post exposure to films was also determined using a LIVE/DEAD Viability/Cytotoxicity Kit (Invitrogen). Approximately 105 cells were seeded per well ...
-
bioRxiv - Molecular Biology 2022Quote: pcDNA5/FRT/TO-FLAG-Angel1 WT was used to generate stable HEK FLp-In T-REx-293 cell lines (Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% Foetal Bovine Serum (FBS) (HEK 293) or Calf Serum (NIH 3T3 and Ptch1−/− MEFs) containing 1% Glutamax (Gibco). All cell lines were grown at 37°C in 5% CO2 and passaged prior to reaching confluence.
-
bioRxiv - Cancer Biology 2023Quote: ... HEK-293 (CRL-1573) cells were obtained from ATCC and maintained in high-glucose (4.5 g/L) DMEM (Gibco 11995073) supplemented with 4 mM L-glutamine ...
-
bioRxiv - Microbiology 2023Quote: ... lentiviruses (for target sequences see S1 File) for shRNA delivery were produced by transfecting HEK-293 cells in a 60mm dish with a 1ml OptiMEM (Gibco) mix containing 15µl of Lipofectamine-2000 (Invitrogen ...
-
bioRxiv - Neuroscience 2023Quote: ... with fewer than 15 passages onto 12-well plates in HEK-293 growth medium (DMEM [Gibco # 10566-061] with 10% FBS [Gibco #16140-071] and 1x Pen Strep [Gibco #15070-63] ...
-
bioRxiv - Neuroscience 2023Quote: ... we plated HEK-293 cells (ATCC catalog # CRL-1573) with fewer than 15 passages onto 12-well plates in HEK-293 growth medium (DMEM [Gibco # 10566-061] with 10% FBS [Gibco #16140-071] and 1x Pen Strep [Gibco #15070-63] ...
-
bioRxiv - Biophysics 2024Quote: The original CC1 construct with super ecliptic pHluorin (SE) A227D (31) or CC1 variants with mutations in S1 were expressed in HEK 293 cells via lipofectamine (Invitrogen). Coverslips with transiently transfected cells were placed into a bath chamber (Warner instruments ...
-
bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-RBD fused with Fc/His tag (S-RBD-His) was produced in 293T cells and purified with Protein A Agarose (Thermo Fisher Scientific). Recombinant human ACE2-ECD fused with Flag/His tag was produced in 293T cells and purified with anti-DYKDDDDK G1 Affinity Resin according to the manufacturer’s instructions (GenScript).
-
bioRxiv - Biophysics 2020Quote: ... and inserted into a human Fc expression vector (Invitrogen). The proteins were expressed in HEK293T cells ...
-
bioRxiv - Biochemistry 2022Quote: ... Human FLP-In™ T-REx™ 293 Cell Lines (Invitrogen™, R78007) expressing hnRNPA1 wild type or D262V mutant cDNAs were generated by cotransfecting the hnRNPA1 cDNAs with a FLP recombinase expression plasmid according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... and transfected into human Flp-In T-REx 293 cells (Invitrogen, Carlsbad, CA). Following hygromycin selection and cell expansion ...
-
bioRxiv - Cell Biology 2020Quote: ... Identified and characterized plasmids containing the ACE2 isoform open reading frames were transfected into the HEK-293 cells using TurboFect reagent (ThermoFisher Corp.). 24 hours post-transfection the cells were harvested and processed for analysis by western blot according to established procedures [21] ...
-
bioRxiv - Cell Biology 2022Quote: ... Ciliogenesis was induced by culturing RPE1 cells in medium without serum during 48 hours. HEK 293 cells (kind gift from F. Causeret, Institut Imagine, Paris) were cultured in DMEM medium (ThermoFisher Scientific) supplemented with 10 % fetal calf serum and antibiotics as previously ...
-
bioRxiv - Cell Biology 2021Quote: ... the HEK 293 BRI3BP-HA cell line was transfected with control or BRI3BP silencer select siRNAs with Lipofectamine RNAi Max (ThermoFisher Scientific), according to manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2022Quote: ... DMEM (for A-431, HEK-293, NTERA-2, SH-SY5Y) or Ham’s F-12K (for A549) media (by Gibco or Euroclone) containing 10% fetal calf serum ...
-
bioRxiv - Microbiology 2022Quote: 100µg of the pCI PR8 NP:WT-C-His or pCI PR8 NP:F346S-C-His plasmids were transiently transfected into 100mL cultures of HEK Expi-293-F cells with ExpiFectamine according to the company protocol (Thermo Fisher). The transfected cells were pelleted (1000rpm ...
-
bioRxiv - Genomics 2021Quote: ... Reporter plasmids (2 μg) were transfected into HEK-293 cells in 6-well plates at ∼60-90% confluency using Lipofectamine 3000 (ThermoFisher Scientific). Cells were harvested after 1 day by aspirating the media and resuspending the cells in 1 mL Trizol reagent (ThermoFisher Scientific) ...
-
bioRxiv - Microbiology 2020Quote: ... HEK-293 cells were maintained at 37°C with 5% CO2 in DMEM/F12 medium (Gibco, Life Technologies, Grand Island, NY) supplemented with 10% heat-inactivated FBS (Gibco) ...
-
bioRxiv - Microbiology 2020Quote: ... HEK-293 cells were maintained at 37°C with 5% CO2 in DMEM/F12 medium (Gibco, Life Technologies, Grand Island, NY) supplemented with 10% heat-inactivated FBS (Gibco) ...
-
bioRxiv - Biochemistry 2022Quote: The pEG BacMam plasmids with WT or mutant Fpn or the empty plasmid were transfected into HEK 293S cells on black wall 96-well microplates coated with poly-D-lysine (Invitrogen/Thermo Fisher). After 2 days ...
-
bioRxiv - Biochemistry 2022Quote: The pEG BacMam plasmids with HsFpn or the empty plasmid were transfected into HEK 293S cells on black wall 96-well microplates coated with poly-D-lysine (Invitrogen/Thermo Fisher). After 2 days ...
-
bioRxiv - Cell Biology 2022Quote: ... MEFs were cultured in the same medium as HEK 293 cells but supplemented with 1x non-essential amino acids (Gibco, 11140050). All cells were kept at 37°C in a 5% CO2 humidified atmosphere.
-
bioRxiv - Cell Biology 2023Quote: ... HEK 293-TrkB cells were treated with drugs prior to cell lysis with cold Pierce RIPA lysis buffer (Thermo Scientific, 89901) supplemented with protease inhibitor set I (Sigma ...
-
bioRxiv - Cell Biology 2023Quote: For proximity ligation assay transfected HEK-293 cells were seeded in an appropriate dilution on Nunc Chamber Slides (Thermo Fisher, USA). After fixation in 4% paraformaldehyde for 15 min at RT ...
-
bioRxiv - Bioengineering 2024Quote: ... and m6A-Tracer-NES plasmid (m6A-Tracer; addgene, #159607) were simultaneously transfected into HEK-293 cells using Lipofectamine 3000 Transfection Reagent (Invitrogen, #L3000008). After 48-h co-transfection ...
-
bioRxiv - Bioengineering 2020Quote: Adherent human embryonic kidney cells (HEKs) were grown in DMEM (Gibco, Gaithersburg, MD) + 10% FBS ...
-
bioRxiv - Genomics 2022Quote: Human HEK 293T cells (ATCC # CRL-3216) were grown in DMEM (ThermoFisher #11995073) supplemented with 10% FBS (ThermoFisher #A3840001 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Human HEK 293T Lenti-X cells were grown in DMEM + GlutaMax (ThermoFisher Scientific) supplemented with 10% FBS ...
-
bioRxiv - Cell Biology 2024Quote: Human Embryonic Kidney (HEK) 293T cells were cultured in DMEM high glucose (Invitrogen), supplemented with 10% fetal bovine serum (GE Healthcare) ...
-
bioRxiv - Microbiology 2020Quote: ... Enzyme and Fc-portion was removed on HIS-Pur Ni-NTA resin (Thermo Fisher Scientific) and Protein G sepharose (GE Healthcare ...
-
bioRxiv - Biophysics 2021Quote: ... and both HRP-conjugated anti-mouse IgG Fc and anti-human IgG Fc were purchased from Invitrogen™ (Waltham ...
-
bioRxiv - Microbiology 2021Quote: ... FXa-HRP conjugated anti-human Fc antibody (05-4220, Invitrogen) was used as a detecting antibody ...
-
bioRxiv - Immunology 2022Quote: ... or IgG1 Fc (human) protein (Thermo Fisher Scientific, Rockford, IL) was added to a final concentration of 10 mg/mL and samples were incubated with gentle mixing for 2 hours at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... then incubated with human Fc receptor coupled to phycoerythrin (ThermoFisher). To set positive gates ...
-
bioRxiv - Immunology 2021Quote: ... or specific for the Fc region of human IgG (Invitrogen), the Fc region of human IgM (Jackson ImmunoResearch Laboratories ...
-
bioRxiv - Microbiology 2021Quote: ... or specific for the Fc region of human IgG (Invitrogen), the Fc region of human IgM (Jackson ImmunoResearch Laboratories ...
-
bioRxiv - Molecular Biology 2021Quote: ... Stable integration of CLIP-UPF1SL into human Flp-InTM T-RExTM-293 cells (Invitrogen) and subsequent maintenance of this stable line was previously described (Kishor et al ...
-
bioRxiv - Immunology 2019Quote: ... Human and rabbit Abs were transiently expressed with the FreeStyle 293 Expression System (Invitrogen). Abs were purified using affinity chromatography (Protein A Sepharose Fast Flow ...