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Citations for Thermo Fisher :
501 - 550 of 7730 citations for Sulfo NHS LC Biotin sodium since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... with different spectral signatures selected for the detection of PfHRP2 and PAN-pLDH were washed with distilled water and activated with Sulfo-NHS (N-hydroxysulfosuccinimide) and EDC (1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride) (Pierce, Thermo Fisher Scientific Inc., Rockford, IL), both at 50 mg/mL ...
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bioRxiv - Synthetic Biology 2023Quote: ... Soluble membrane proteins were isolated from homogenized and sonicated Expi 293F cells followed by biotinylation with Sulfo-NHC-SS-Biotin (Thermo Fisher Scientific, 21331) and stored in PBS at −80 °C ...
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bioRxiv - Immunology 2022Quote: ... were washed with 100 mM monobasic sodium phosphate pH 6.2 and activated by addition of Sulfo-N-Hydroxysulfosuccinimide (Thermo Fisher Scientific) and 1-Ethyl-3-(3-dimethylaminopropyl ...
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bioRxiv - Biochemistry 2022Quote: SOSIP trimers were randomly biotinylated following manufacturer’s guidelines using the EZ-Link NHS-PEG4-Biotin kit (Thermo Fisher Scientific). The Pierce Biotin kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... a PDL-coated mylar film dish was biotinylated by incubating NHS-biotin (Thermo Fisher Scientific, 200 4 µg/ml) for 3h ...
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bioRxiv - Immunology 2022Quote: ... During this time the primary antibody was biotinylated according to manufacturer protocol (Thermo Scientific EZ-Link NHS-PEG4-Biotin). Blocking solution was removed and 100 μl/well of biotinylated mAb 1C7C7 ...
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bioRxiv - Bioengineering 2023Quote: ... free NHS-AF647 was eliminated with 0.5mL 7kDa MWCO Zeba Dye and Biotin Removal Spin Columns (Thermo Fisher Scientific). Conjugated antibodies were used immediately or stored at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Heparin−biotin sodium salt was immobilized on Pierce™ Streptavidin Coated Plates (Thermo Fisher Scientific). The volume used to immobilize Heparin-biotin (100 μg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... PltB and Neu5Gc-IgY was achieved by NHS mediated biotin tagging at 20:1 molar ratio as per manufacturer’s protocol (Thermo scientific, EZ-Link Sulfo-NHS-LC Biotin ...
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bioRxiv - Cancer Biology 2019Quote: ... These α1-α2 phage phage libraries were captured with either biotinylated Fc-iNKG2D.YA or Fc-iNKG2D.AF protein (EZ-LinkTM NHS-Biotin Kit, Thermo Fisher #20217) and enriched by cycling through four rounds of selection with increasing concentrations of non-biotinylated Fc-wtNKG2D competitor ...
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bioRxiv - Cancer Biology 2022Quote: ... according to the manufacturer’s protocol and excess NHS-biotin was removed with 0.5 mL Zeba Spin Desalting Columns (Thermo Fisher, 87766). Bispecific CSANs were then formed using the biotinylated αCD3 scFv monomer and the αB7-H3 scFv monomer at a 1:1 ratio ...
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bioRxiv - Immunology 2020Quote: Purified recombinant S2P or RBD were biotinylated at a theoretical 1:1 ratio using the Easylink NHS-biotin kit (Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Serum ELISAs were performed using randomly biotinylated SOSIP trimers using the EZ-Link NHS-PEG4-Biotin kit (Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2023Quote: Porcine brain tubulin was isolated using the high-molarity PIPES procedure as described (Castoldi and Popov, 2003) and then labeled with biotin- or Dylight-405 NHS-ester (Invitrogen) as described (http://mitchison.hms.harvard.edu/files/mitchisonlab/files/labeling_tubul in_and_quantifying_labeling_stoichiometry.pdf) ...
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bioRxiv - Molecular Biology 2023Quote: ... γ-TuRC was biotinylated prior to elution by incubation for 1 hour on ice in CSF-xB 2% (w/w) sucrose supplemented with 40 μM NHS-PEG4-biotin (ThermoFisher). The elution was concentrated using a 100 kDa MWCO spin concentrator (Amicon) ...
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bioRxiv - Biochemistry 2023Quote: ... Hisα1-subCD3-WT (Legrand et al., 2020) was biotinylated with EZ-Link™ NHS-PEG4-Biotin (Thermo Scientific™ A39259) in a 1:1.5 ratio at 25°C for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... through a sulfo-SMCC (Thermofisher, 22322) hetero-bifunctional crosslinker that linked the 3’ thiol on the top strand to an amine moiety of the Protein G ...
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bioRxiv - Biophysics 2020Quote: Oocytes expressing Wild-type Cx30 or Cx30G11R were washed out with cold PBS and then incubated with PBS containing 0.5 mg/mL EZ-LinkTM NHS-Biotin (Thermo Fisher Scientific) for 10 minutes at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... the antibody-coupled beads were washed three times in 100 μl PBS-T before labelling the antibodies with a 150x molar excess of EZ-Link-NHS-PEG4-Biotin (21329, Thermo Scientific) dissolved in DMSO (276855 ...
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bioRxiv - Biophysics 2022Quote: The full-length CcdA and CcdA peptides of different lengths (Table S1) were biotinylated with EZ-linkTM NHS-Biotin (ThermoFisher Scientific) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Typhimurium was grown in BHI broth until OD600:1 and then biotinylated with EZ-link NHS-Biotin reagent (Thermo Fisher Scientific). After washing ...
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bioRxiv - Biochemistry 2022Quote: Purified protein at a concentration of 1 mg/ml was chemically biotinylated using EZ link NHS-PEG4-Biotin (Thermo Scientific, A39259) at a 10-30 times molar excess ...
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bioRxiv - Biophysics 2022Quote: ... Briefly, ligands (DinB, DinBΔPAD, and DinB-PAD) were biotinylated using the biotinylation kit EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific). The biotin label was freshly dissolved in H2O ...
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bioRxiv - Microbiology 2023Quote: ... 100 μl of His-GLPR at 25 μM was incubated with 20x molar excess of EZ-Link NHS-PEG4-Biotin (Thermo Scientific) following supplier instructions ...
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bioRxiv - Neuroscience 2023Quote: The incorporation of biotin moieties for downstream detection of nanobody constructs was achieved using a 5-fold molar excess of EZ-link-NHS-PEG4-biotin (#21330, Thermo Fisher) with nanobody constructs in 50mM sodium carbonate buffer ...
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bioRxiv - Developmental Biology 2024Quote: Transfected COS7 cells on a 100-mm dish were biotinylated by incubation in 1 mg/ml NHS-SS-Biotin (21331, Thermo Scientific), diluted in 1× PBS containing 1 mM MgCl2 and 0.1 mM CaCl2 (PBS–MC ...
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bioRxiv - Biophysics 2023Quote: ... MSP1E3D1 was expressed and purified as previously described (Ritchie 2009) and biotinylated with EZ-Link NHS-polyethylene glycol 4 (PEG4)-Biotin (Thermo Fisher) per manufacturer instructions ...
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bioRxiv - Biophysics 2023Quote: ... Protein at 2 mg/mL in interaction buffer (150 mM NaCl 50 mM Phosphate pH 8) was treated with 1.25 mM Biotin LC-Hydrazide (Thermofisher) and 5 mM EDC for two hours then dialyzed against the initial buffer ...
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bioRxiv - Biochemistry 2024Quote: ... NHS-5/6-carboxyfluorescein (NHS-fluorescein, Thermo Fisher 46410), NHS-Oregon Green (Thermo Fisher O6147) ...
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bioRxiv - Cancer Biology 2019Quote: ... DiI-VLDLs were dialyzed into PBS containing 0.05 M sodium borate (pH 8.5) and incubated with DyLight 650 NHS-Ester (ThermoFisher Scientific) at room temperature for 1.5 h ...
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bioRxiv - Cancer Biology 2021Quote: Cells were grown on 6-well plates until 80-90% confluency and cell surface proteins were biotinylated with EZ-Link NHS-PEG4-Biotin (ThermoFisher Scientific, #21330) at 0.5 mg/ml in HBSS (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Each well was washed with PBS and then incubated for one hour at 4°C while rocking with 1 mg/mL EZ-Link NHS-SS-Biotin (Thermo Fisher Scientific) in PBS ...
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bioRxiv - Immunology 2021Quote: ... S-2P and RBD were biotinylated protein at a theoretical 1:1 ration using the Easylink NHS-biotin kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then placed on ice and incubated twice with 0.75 mg/ml NHS-SS-biotin in PBS (Thermo Scientific, Rockford, Illinois) for 30 min while bubbling with 95% O2 and 5% CO2 ...
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Revisiting the role of Toxoplasma gondii ERK7 in the maintenance and stability of the apical complexbioRxiv - Microbiology 2021Quote: ... The NHS-Ester staining (Thermofisher, DyLight™ 488 NHS Ester) was used at 5μg/mL and incubated 1 hour in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Gels were functionalized with 0.1M sulfo-succinimidyl 6-(4’-azido-2’-nitrophenylamino) hexanoate (Sulfo-SANPAH) (Cat# 22589, Thermo Scientific) dissolved in HEPES before overnight incubation in collagen type-I solution (Catalogue # A1048301 ...
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bioRxiv - Molecular Biology 2022Quote: An N-hydroxysuccinimidyl (NHS) ester of Alexa488 fluorochrome (NHS-Alexa488, ThermoFisher) was used to fluorescently label purified Pae87 ...
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bioRxiv - Biochemistry 2022Quote: ... mono-reactive NHS ester and NHS-ester conjugated Atto488 (Thermo Fisher) were used ...
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bioRxiv - Immunology 2021Quote: ... using covalent NHS-ester linkages via EDC and NHS (Thermo Scientific) as described previously (Brown et ...
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bioRxiv - Neuroscience 2021Quote: ... Biotin molecules were labeled at the N-terminal primary amino group (-NH2) of the bait proteins using N-hydroxysuccinimide (NHS)-biotin (ThermoFisher Scientific, Waltham, MA) in PBS at pH 6.5 ...
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bioRxiv - Cell Biology 2019Quote: ... 0.2 mg/ml Sulfo-SANPAH (Thermo Scientific, 22589) and 2 mg/ml N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC ...
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bioRxiv - Biophysics 2019Quote: ... Gels were activated with sulfo-SANPAH (Thermo Scientific) under UV light and coated with 0.15 mg/ml collagen I (Corning).
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bioRxiv - Cell Biology 2022Quote: ... 1 mg/mL sulfo-SANPAH (Thermo Fisher Scientific) in deionized water was pipetted onto the hydrogel ...
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bioRxiv - Cell Biology 2023Quote: ... Sulfo-SDA (sulfosuccinimidyl 4,4’-azipentanoate) (Thermo Scientific Pierce) cross-linking reaction was a two-step process ...
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bioRxiv - Biophysics 2021Quote: ... solution (pH 8) and incubated in 0.1M sodium bicarbonate solution (pH 8) with 2 uM Alexa Fluor™ 647 NHS Ester (Invitrogen, A37573) for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... followed by staining in 0.5 ml 0.2 M sodium bicarbonate containing 50 mg/ml Alexa Fluor 488 NHS Ester (succinimidyl ester) (Thermo Fisher Scientific #A20100) for 30 min at RT ...
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bioRxiv - Biochemistry 2022Quote: ... Synthetic peptides were coupled to rabbit serum albumin (RSA) with the linker Sulfo-m-maleimidobenzoyl-N-hydroxysuccinimide ester (Sulfo-MBS, Thermo Scientific) in a carrier (1):linker (50):peptide (50 ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for one hour with SULFO-TAG conjugated anti-human IgG for mAbs (MSD) or SULFO-TAG conjugated polyclonal anti-human IgG+A+M (Thermo Fisher) for serum samples ...
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bioRxiv - Plant Biology 2021Quote: All solvents used for the processing of xylem sap and the resulting proteins were liquid chromatography-mass spectrometry (LC-MS) or high-performance liquid chromatography (HPLC) grade and obtained from Fisher Scientific (Hampton, NH, USA). 400 μL xylem sap were lyophilized (Freeze dryer ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 0.2 mM MgCl2 and 1 mM EGTA.66We prepared fluorescent tubulin (ATTO488- and ATTO565-labeled tubulin; AttoTech AD488-35 and AD565-35, AttoTech) and biotinylated tubulin (NHS-biotin, 21338, Thermo Scientific, Waltham, MA, USA) as previously described.67 In brief ...