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Citations for Thermo Fisher :
451 - 500 of 7730 citations for Sulfo NHS LC Biotin sodium since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... branched PEI was conjugated to the PLP surface by reaction with EDC and sulfo-NHS (Thermo Fisher Scientific, Bedford, MA). PLPs were washed again sequentially with 1 mM NaCl and DI H2O ...
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bioRxiv - Immunology 2023Quote: ... 25 µg of Spike protein was biotinylated according to the instructions of EZ-LinkTM Micro Sulfo-NHS-LCBiotinylation Kit (ThermoFisher). Then fluorescent (APC ...
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bioRxiv - Cell Biology 2023Quote: ... NeutrAvidin bead acetylation was necessary to stop the Neutravidin being cleaved from the agarose beads during on-bead digestion and performed prior to the day of pulldown by two 30 min incubations of beads with 10 mM Sulfo-NHS acetate (ThermoFisher) on a rotating wheel followed by quenching in 90 mM Tris-HCl pH7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... and water Optima™ LC-MS Grade were obtained from Fisher Scientific (Hampton, NH, USA). Rabbit anti-Von Willebrand factor (vWf ...
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bioRxiv - Cancer Biology 2021Quote: ... The concentrated extract was transferred to an LC-MS autosampler vial (Fisher Scientific, Hampton, NH) and injected right away.
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bioRxiv - Cancer Biology 2023Quote: ... LC-MS grade acetic acid and formic acid were purchased from Fisher Scientific (Hampton, NH). All chemicals and solvents used in this study were HPLC or reagent grade unless otherwise noted.
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bioRxiv - Neuroscience 2021Quote: ... 5 μl of tracer cocktail (10 mg/ml EZ-Link NHS-Biotin (Thermo Fisher: 20217) and 10 kDa Dextran Alexa Fluor 488 (Thermo Fisher ...
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bioRxiv - Biophysics 2022Quote: ... and were conjugated with biotin and silicon rhodamine (SiR) NHS esters (Invitrogen: B1582, Spirochrome: SC003) to produce “SiR-biotin-Fab” ...
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bioRxiv - Immunology 2023Quote: ... with 20× molar excess of EZ link™ NHS-PEG4-Biotin (Thermo Fisher Scientific, 210901BID), in PBS ...
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bioRxiv - Microbiology 2024Quote: Recombinant spike proteins were biotinylated with EZ-Link™ NHS-PEG4-Biotin (ThermoFisher, Cat. # A39259) for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-PCDH15 8D1 capture antibody was biotinylated using NHS-PEG4-biotin (Thermo Fisher CAS# 21330) and anti-LHFPL5 antibody was labeled with Alexa647 using a Zip Alexa Fluor Rapid Antibody Labeling Kit (Thermo Fisher CAS#Z11235) ...
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bioRxiv - Immunology 2024Quote: ... recombinant spike proteins were biotinylated with EZ-Link™ NHS-PEG4-Biotin (ThermoFisher, Cat. # A39259) for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher ...
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bioRxiv - Biophysics 2020Quote: Sulfo-Cy3 (Invitrogen) linked to the maleimide thiol-reactive group ...
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bioRxiv - Biophysics 2021Quote: ... Unreacted amine groups on the silane molecules were capped using NHS-sulfo acetate (10 mg/ml for 30 mins; Thermo Fisher). PEG-functionalized AFM cantilevers were incubated in 1 mg/ml Bovine Serum Albumin 55 overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Pierce premium grade N-hydroxysulfosuccinimide (sulfo-NHS, PG82071) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, 22980) were purchased from Thermo Fisher Scientific.
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bioRxiv - Biochemistry 2021Quote: ... and water were Optima™ LC-MS Grade and obtained from Fisher Scientific (Hampton, NH, USA). Ethanol LiChrosolv® Grade was obtained from Merck KGaA (Darmstadt ...
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bioRxiv - Microbiology 2021Quote: ... 5 mM MgCl2) before biotinylation using EZ-Link NHS-PEG4-biotin (Thermo Fisher Scientific, Waltham, Massachusetts) in equimolar concentration ...
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bioRxiv - Bioengineering 2021Quote: ... IgM or C1q samples were biotinylated using NHS-PEG4-biotin EZ-link kit (Thermo Fisher Scientific) and manufacturer conditions ...
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bioRxiv - Microbiology 2020Quote: ... GP was directly labeled using the amine-specific biotinylation reagent EZ-Link NHS-PEG4-Biotin (ThermoFisher), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Smurf1 HECT NT protein was biotinylated using the No-Weight NHS-PEO4-Biotin kit (Thermo Fisher) due to its compatibility with Super Streptavidin (SSA ...
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bioRxiv - Biochemistry 2020Quote: ... All contrast agents were activated for bioconjugation with purified L-PGDS using by addition of 100mM EDC and 250mM sulfo-NHS dissolved in amine- and carboxyl-free MES buffer (Thermo Scientific™) with pH adjusted to 6.0 to a final ratio of 1:5 [50] ...
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bioRxiv - Cell Biology 2022Quote: ... cells were shifted to ice and incubated for 30 min with cold biotinylation solution (Thermo Fisher Scientific EZ-link Sulfo-NHS 21327) diluted in PBS (0.17-mg/mL final concentration) ...
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bioRxiv - Immunology 2022Quote: ... A33 and B5 antigens were coupled via carboxyl chemistry to Magplex® fluorescently bar-coded beads (Luminex Corporation) with sulfo-NHS and EDC (Thermo Fisher) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Bioengineering 2024Quote: ... Capture antibodies (provided in Supplementary Table 1) were biotinylated using the EZ-Link micro Sulfo-NHS-biotinylation kit from (Thermo Fisher Scientific). The biotinylated antibodies were then diluted to 10 μg/mL in a 1 % BSA solution and added to the grids for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... LC-MS grade acetonitrile with 0.1% formic acid in water were purchased from Fisher Scientific (Hampton, NH). Guanidine hydrochloride ...
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bioRxiv - Bioengineering 2021Quote: ... nanoparticles were suspended in saline (0.9% Sodium Chloride, NaCl, Crystalline, Fisher Scientific, Hampton, NH, USA) or Vasculife VEGF basal cell media with 10% Fetal Bovine Serum (LL-0003 ...
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bioRxiv - Biophysics 2020Quote: ... Sulfo-SANPAH (ThermoFisher Scientific) solution dissolved in 100 mM HEPES was added on top of the substrates and they were exposed to UV for 2 minutes ...
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bioRxiv - Biophysics 2022Quote: ... Sulfo-SANPAH (Thermo Fisher Scientific Inc. ...
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bioRxiv - Immunology 2022Quote: BG505 SOSIP trimers were randomly biotinylated using the EZ-Link NHS-PEG4-Biotin kit (Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then biotinylated using NHS-PEG4-Biotin (125 µM unless otherwise specified, ThermoFisher Cat. No. A39259) diluted in PBSCMA to achieve a total volume of 50 µL per well for 30 minutes at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... the respective ligands were biotinylated using the biotinylation kit EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific). The biotin label was resolved in H2O and directly added to the protein solution in a final molar ratio of 1:1 in 25 mM MES ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μg of recombinant 6xHis-4xSUMO2-Strep was biotinylated using EZ-link NHS-PEG4-Biotin (Thermo Fisher) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Recombinant gH/gL proteins were biotinylated using the EZ-Link NHS-PEG4-Biotin Kit (ThermoFisher, catalog #21330) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... we covalently labeled cell-surface proteins using a membrane-impermeable biotinylation reagent (NHS-SS-biotin; Thermo Fisher) as described previously (Yamada et al. ...
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bioRxiv - Biophysics 2019Quote: ... a six-fold molar excess of photo-cleavable NHS-biotin cross-linker (Fisher Scientific, # NC1042-383, USA) was added to the Fabs (0.28 mg/mL ...
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bioRxiv - Biophysics 2020Quote: ... The respective ligands were biotinylated using the biotinylation kit EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific). The biotin label was freshly resolved in H2O ...
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bioRxiv - Cell Biology 2023Quote: ... EZ-Link™ Sulpho-NHS-Biotin (1 mg/ml in PBS buffer, pH 8; Thermo fisher; 21217) was added (or not ...
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bioRxiv - Bioengineering 2022Quote: ... NHS-PEG4-Biotin (catalog no. 21330) and HRP-conjugated streptavidin (catalog no. N100) were purchased from Thermofisher. DAB (catalog no ...
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bioRxiv - Biochemistry 2024Quote: ... TcA(914Cys) and TcA(1279Cys) were biotinylated using EZ-link NHS-PEG12-Biotin (ThermoFisher, cat. no. A35389). Biotinylation was performed in labelling buffer simultaneous to the labelling reaction with BFL and Atto647N ...
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bioRxiv - Neuroscience 2024Quote: ... The cell culture medium was removed and cells were suspended with 1 mL ice-cold PBS and then treated with 1 mL sulfo-N-hydroxysuccinimide-SS-biotin (Thermo Scientific) (1 mg/mL in PBS ...
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bioRxiv - Biophysics 2022Quote: ... The terminal free amine on the spacer was reacted with the N-hydroxysuccinimide (NHS) ester group of sulfo-SMCC (Thermo Fisher Scientific, 22622). The maleimide group of sulfo-SMCC was subsequently conjugated to 3’ thiol modified DNA oligonucleotides as described19 ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were incubated for 30 minutes with 0.5 mg/ml sulpho-NHS-SS-biotin (Thermo Fisher Scientific) in PBS++ ...
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bioRxiv - Immunology 2022Quote: ... Biotinylation of Spike or RBD proteins was performed using the EZ-Link™ NHS-PEG4-Biotin (Life Technologies) using a 3-fold molar excess of reagent and using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein ligands (Red1288-345 and Iss1) were biotinylated using EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific). Biotin-labeled proteins were immobilized on the streptavidin (SA ...
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bioRxiv - Immunology 2023Quote: ... purified SARS-CoV-2 Spike and RBD were biotinylated using the EZ-Link NHS-Biotin (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... CALHM2 and CALHM4 proteins were chemically biotinylated using amine-reactive EZ-Link™ NHS-PEG4-Biotin (ThermoFisher Scientific). For this purpose ...
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bioRxiv - Biophysics 2022Quote: ... 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC, Catalog No. 22980) and Sulfo-NHS (N-hydroxysulfosuccinimide, Catalog No. 24510) were purchased from Thermo Scientific (Waltham, MA, US). Phosphate-Buffered Saline (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... Washed beads were resuspended in a mix of 190 µl of Buffer 1 and 10 µl of 100 mM PierceTM Sulfo-NHS-Acetate (Thermo Scientific, dissolved in DMSO), and incubated for 1 hour at room temperature to acetylate free amines (36) ...