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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Siglec 5 Human HEK293 His Flag Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: HEK293 and GL261 cells were cultured in DMEM (Gibco, #31885) with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... GKO HEK293 cells were maintained in DMEM (Gibco Cat# 11995065) containing 10% FBS (Gibco Cat# 16000044) ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 Flp-In T-Rex cells (Thermo Fisher Scientific, R78007) were transfected with pcDNA5/FRT/TO-GFP (see below ...
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bioRxiv - Developmental Biology 2023Quote: ... HEK293 and P19 cells were transfected by Lipo2000 (Invitrogen, 11668500) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were transfected with the NeonTM transfection kit (Invitrogen) according to the manufacturer’s description ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 Flp-In T-REx cells (Thermo Fisher Scientific, R78007) with SBP-tagged eIF4A1 integrant 15 were seeded in a 10-cm dish and cultured for 3 d in the presence of 1 μg/ml tetracycline ...
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bioRxiv - Systems Biology 2023Quote: ... HEK293 cell lines were lifted using TrypLE Express (Thermo Fisher) and resuspended in media supplemented with the appropriate volume of cADDis BacMam ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293 in Dulbecco’s modified Eagle Medium (DMEM, Life Technologies #11965118); T-47D in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Cell Biology 2023Quote: Flp-In™ T-REx™ HEK293 cells (Invitrogen, R78007) containing a single genomic FRT site and stably expressing the Tet repressor were cultured in DMEM medium supplemented with 10% FBS ...
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bioRxiv - Developmental Biology 2024Quote: HEK293 cells were transfected using Lipofectamine 3000 (Invitrogen, Cat# L3000008) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Flp-In T-Rex HEK293 cell lines (Thermo Fisher Scientific) expressing mitochondrial-relocated golgin-BirA* fusions (from John Shin ...
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bioRxiv - Biochemistry 2024Quote: ... GKO HEK293 cells were maintained in DMEM (Gibco Cat# 11995065) containing 10 % FBS (Gibco Cat# 16000044) ...
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bioRxiv - Cell Biology 2024Quote: FlpIn CHO and HEK293 cell lines were purchased from Invitrogen and maintained in Dulbecco’s Modified Eagle’s Medium supplemented with 5% Foetal Bovine Serum (ThermoFisher Scientific (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: Flp-In T-Rex HEK293 cells (Invitrogen, Thermo Fisher Scientific) were co-transfected with plasmids pOG44 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: Flp-In T-Rex HEK293 cells (Invitrogen, Thermo Fisher Scientific) were co-transfected with plasmids pOG44 (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... HEK293 GnTI- cells were cultured in Freestyle 293 medium (Invitrogen) supplemented with 2% FBS on a shaker at 37 °C in the presence of 8% CO2 to a density of 3 × 106 cells per ml ...
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bioRxiv - Neuroscience 2023Quote: ... Stable HEK293 lines were generated by lipofection (Lipofectamine 2000, ThermoFisher) of ∼80% confluent cells with 2 µg of linearized expression plasmid overnight according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: HEK293T cells were transfected with FLAG-Empty Vector (FLAG-EV) and FLAG-SP140 plasmids using Lipofectamine 2000 (Invitrogen) for 48 hours ...
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bioRxiv - Cell Biology 2019Quote: ... The resulting PCR product was cloned between 5’ KpnI and 3’ EcoRI sites of the pMT/V5-His vector (Invitrogen). In-frame Tag-RFP-T gene was then introduced at the 3’ end of γ-tubulin gene between 5’ EcoRI and 3’ NotI sites ...
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bioRxiv - Cell Biology 2019Quote: ... The resulting PCR product was then inserted into the 5’ SpeI and 3’ EcoRI sites of the pMT/V5 His-B vector (Invitrogen) containing in-frame mTurquoise2 gene at the 5’ end ...
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bioRxiv - Molecular Biology 2022Quote: ... with FLAG sequence flanking at 5’ end of the primer and cloned in Hind III and Xba I site of pCDNA3.1V5-His (Invitrogen, USA). Clones were sequenced with T7 and BGH primer using ABI BigDyeTerminator V3.1 cycle sequencing kit followed by automated sequencing in ABI 3130 Genetic Analyzer according to manufacturer’s protocol.
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bioRxiv - Developmental Biology 2022Quote: ... F 5’-GAACTGTCCAGATGCCCTTCCAGTT-3’ and R 5’-GCATCTGGACAGTTCTGGGAAGCCCG-3’) was cloned by PCR and ligated into the pEF6/V5-His TOPO plasmid vector (Invitrogen). FBLN7-V5-His vector was transfected into CHO cells (FBLN7-CHO ...
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bioRxiv - Biochemistry 2019Quote: ... blocked with 5% milk in TBST and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (ThermoFisher MA1-21315, 1:3000). Blots were incubated with primary antibodies overnight at 4 °C with rocking and were then washed (3×5min ...
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bioRxiv - Biochemistry 2024Quote: ... C2C12 cells transfected with EGFP-HRas WT/C181S/C184S in 35mm dishes were treated with 15d-PGJ2-Biotin (5 µM) in DMEM Hi Glucose medium (Gibco) supplemented with 1% Penicillin-streptomycin-Glutamine (Gibco ...
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bioRxiv - Plant Biology 2022Quote: ... His-tagged ChElp2 was pulled-down with Dynabeads™ His-Tag (Thermo Fisher Scientific, Massachusetts, USA) and the subsequent western blot analyses were performed with anti-His (Sigma ...
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bioRxiv - Cell Biology 2019Quote: Recombinant His-tagged EB1-GFP and His-tagged SEPT5 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.6-0.8 and induced with 0.2 mM IPTG for 16 h at 18°C for His-tagged-EB1-GFP or cultures were grown to OD600 of 0.4-0.6 and induced with 1 mM IPTG for 5 h at 23°C for His-tagged SEPT5 ...
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bioRxiv - Immunology 2024Quote: ... the lysate supernatant containing His-tagged proteins was affinity-purified with His-Tag magnetic beads (Invitrogen) and used for pull-down assays ...
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bioRxiv - Neuroscience 2023Quote: ... SH-SY5Y cells were transfected with either an empty vector or with a vector expressing His-tagged human p35 (gift from Dr. Harish Pant) using Lipofectamine 3000 (Life Technologies, Carlsbad, CA). Two days after the transfection ...
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bioRxiv - Developmental Biology 2022Quote: HeLa cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) with Glutmax and 5% FCS (Gibco) and passaged every few days ...
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bioRxiv - Immunology 2023Quote: All single cell suspensions were prepared in Hank’s buffer (1×HBSS, 5% FCS, 10mM HEPES; all ThermoFisher, Life Technologies). For this ...
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bioRxiv - Immunology 2023Quote: All single cell suspensions were prepared in Hank’s buffer (1×HBSS, 5% FCS, 10mM HEPES; all ThermoFisher, Life Technologies). For this ...
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bioRxiv - Biochemistry 2023Quote: ... The established stable cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Nacalai Tesque) supplemented with 4∼5% fetal calf serum (FCS; Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: HeLa cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) with Glutmax and 5% FCS (Gibco) and passaged every few days ...
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bioRxiv - Microbiology 2020Quote: Dynabeads His-Tag Isolation & Pulldown (Invitrogen) were washed in PBS-TH (PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-His (Thermo Fisher scientific, rd230540a), anti-EZH2 (Cell signaling #5246 ...
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Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cellsbioRxiv - Cell Biology 2022Quote: ... pcDNA3.1/V5-His (Thermo Fisher Scientific), pCLXSN-GFP (Reiley et al 2005) ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% HI-FBS (Gibco) at 37°C in the presence of 5% CO2 ...
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bioRxiv - Genetics 2019Quote: ... 2% HI horse serum (Thermo Fisher), and 1% P/S ...
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bioRxiv - Immunology 2021Quote: ... magnetic beads (Dynabeads His-Tag, Invitrogen) were coated with histidine tagged S protein according to the manufacturers’ instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Hi-Di Formamide (Applied Biosystems, California) and 500 liz size standard (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... with 10% HI-FBS (Gibco, #10082147) and 1% Penicillin-Streptomycin (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... Stock anti-His antibody (Thermo Fisher) was diluted to 70 μg/mL in BRB80 buffer ...
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bioRxiv - Microbiology 2022Quote: ... Mouse-anti-His (HIS.H8) antibody (Invitrogen) (for eGFP/His-tagged NP protein co-immunoprecipitation ...
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bioRxiv - Neuroscience 2020Quote: ... containing 10% HI FBS (Life Technologies) and 35 µg/ml Ascorbic acid (Sigma) ...
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bioRxiv - Immunology 2021Quote: 6x His tag monoclonal antibody (Invitrogen) was coated at 2 μg/mL in PBS onto half-area 96-well high binding plates (Corning ...
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bioRxiv - Immunology 2021Quote: 6x His tag monoclonal antibody (Invitrogen) or streptavidin (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2022Quote: ... + 10% HI-Horse Serum (Gibco, 26050088) + 0.8% Pen/Strep (Gibco ...
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bioRxiv - Microbiology 2021Quote: Plasmid based on pBAD/His (Invitrogen) or pHT01 (MoBiTech ...
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bioRxiv - Microbiology 2020Quote: ... the pIB/V5-His vector (Invitrogen) and insert egfp fragment were amplified by PCR ...
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bioRxiv - Biochemistry 2020Quote: ... Anti‐His tag monoclonal antibody (Invitrogen) was used in western blot analysis.