Labshake search
Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Siglec 5 Human HEK293 His Flag Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
-
bioRxiv - Cell Biology 2023Quote: ... The plasmid pcDNA4-B–hVMP1–V5/His was obtained by inserting the human VMP1 sequence (NM_030938.5) into pcDNATM4/V5-HisB (Invitrogen catalog number V861-20). pLenti–hVMP1–FLAG was obtained by inserting the oligonucleotide MCS-flag into pENTR1A (Addgene Plasmid #17398) ...
-
bioRxiv - Cancer Biology 2020Quote: Cells were maintained at 37 °C under humidified 5% CO2 in DMEM medium supplemented with 10% FCS (Invitrogen) and 2 mM glutamine ...
-
bioRxiv - Microbiology 2020Quote: rCEACAM-His was attached to nickel dynabeads (DB; Dynabead His-tag pull-down & isolation, Invitrogen) following standard protocols ...
-
bioRxiv - Microbiology 2019Quote: ... Plates were washed 6 times and incubated with horseradish peroxidase (HRP)-conjugated mouse anti-human IgG Fc secondary antibody (Cat# 05-4220; ThermoFisher Scientific) diluted 1:1000 in 100 µl blocking buffer for 1 h at room temperature or 37°C ...
-
bioRxiv - Immunology 2021Quote: ... residues 306–577 of MERS-CoV S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
-
bioRxiv - Biochemistry 2021Quote: ... Detection was enabled by addition of 100 µL Goat anti-Human IgG Fc secondary antibody conjugated to horseradish peroxidase (ThermoFisher #A18817), diluted 1:50,000 into PBSM and incubated in the above plate shaker for 1 h at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Serum samples at varying dilutions were incubated for 2 h followed by detection by a goat anti-human Fc-HRP secondary antibody (ThermoFisher Scientific). The plasma concentration of AvFc was calculated by interpolating from a standard curve ...
-
bioRxiv - Genomics 2022Quote: ... Microarrays were then washed (three times with PBS-T for 1 minute) and peptide binding was detected with isotype-specific secondary goat anti-human IgG (Fc) DyLight680 (ThermoFisher Scientific) and goat anti-human IgA (alpha chain ...
-
bioRxiv - Immunology 2020Quote: ... or residues 319–591 of SARS-CoV-2 S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV-2 RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
-
bioRxiv - Cell Biology 2023Quote: ... were cultured in complete RPMI (10% FCS, 1% Penicillin/Streptavidin, 1% HEPES) supplemented with recombinant human M-CSF (10 ng/mL, Life Technologies) for 7-10 days to differentiate monocyte-derived macrophages (MDM) ...
-
bioRxiv - Immunology 2023Quote: ... The cells were washed three times with flow staining buffer and labeled with a PE-conjugated anti-Human IgG Fc antibody (Thermo Fisher) for 30 mins ...
-
bioRxiv - Immunology 2024Quote: ... Cells were then washed 3x in FACS Buffer and stained on ice for 1 hour with goat anti-Human IgG Fc Alexa Fluor™ 647 secondary antibody (ThermoFisher), protected from light ...
-
bioRxiv - Cell Biology 2024Quote: ... human TIM4-Fc protein (FUJIFILM Wako Pure Chemical Corporation) was biotinylated with EZ-Link™ Maleimide-PEG11-Biotin (Thermo Fisher Scientific) and conjugated to Dynabeads™ MyOne™ Streptavidin C1 magnetic beads (65001 ...
-
bioRxiv - Microbiology 2022Quote: ... Surface PCDH1 was stained using human anti-EC7 mAb-3677 (5 µg/mL) followed by anti-human Alexa FluorTM 555 (ThermoFisher) for 1 h at 4°C each ...
-
bioRxiv - Biophysics 2019Quote: ... FLAG beads (Thermofisher) were washed into assay buffer (100 mM Tris ...
-
bioRxiv - Cell Biology 2024Quote: ... FLAG (Invitrogen, 740001); V5 (Abcam ...
-
bioRxiv - Developmental Biology 2019Quote: HEK293 (Flp-In™-293 Cell Line, Thermo Fisher Scientific) were grown in DMEM supplemented with 10% FBS ...
-
bioRxiv - Cancer Biology 2019Quote: HEK293-SUMO3m cells were grown in DMEM (Thermo Fisher Scientific) containing light (0Lys ...
-
bioRxiv - Biochemistry 2020Quote: HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (Invitrogen) with 5% (v/v ...
-
bioRxiv - Biochemistry 2019Quote: ... HEK293 cells were maintained in DMEM (Thermo Fisher Scientific, 10569010) supplemented with 10% FBS and penicillin/streptomycin ...
-
bioRxiv - Neuroscience 2019Quote: HEK293 cells or FreeStyle 293F cells (Invitrogen, Waltham, MA, USA) were cultured in DMEM medium with 10% FBS and 1% Pen-Strep at 37°C with 5% CO2 ...
-
bioRxiv - Molecular Biology 2020Quote: ... and pHelper62 into HEK293 cells using Lipofectamine LTX (Invitrogen 15338030) following standard protocols62 ...
-
Structural investigation of ACE2 dependent disassembly of the trimeric SARS-CoV-2 Spike glycoproteinbioRxiv - Biophysics 2020Quote: ... The construct was transiently transfected into HEK293 cells (Thermo Fisher) with PEI MAX (Polysciences ...
-
bioRxiv - Neuroscience 2020Quote: HEK293 cells were maintained in DMEM (High Glucose DMEM; Gibco) supplemented with 10% (v/v ...
-
bioRxiv - Molecular Biology 2021Quote: HEK293 Flp-In T-Rex cells (Thermo Fisher Scientific, R78007) were cultured in DMEM + GlutaMAX-I (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... HEK293 was grown in DMEM (Thermo Fisher, USA, Cat# 11995065) supplemented with 10% FBS (HyClone ...
-
bioRxiv - Cancer Biology 2020Quote: ... VCaP and HEK293 were grown in DMEM with Glutamax (Gibco); LNCaP ...
-
bioRxiv - Molecular Biology 2021Quote: Flp-In™ T-REx™ HEK293 parental cells (Invitrogen) were seeded at 1×106 cells per 10 cm culturing dish in 15 ml media (DMEM High Glucose ...
-
bioRxiv - Immunology 2020Quote: ... HEK293 cells were maintained in DMEM (Thermo Fisher cat. 11965) supplemented with 10% FBS ...
-
bioRxiv - Biochemistry 2021Quote: 0.3×106 HEK293 cells in DMEM media (Gibco #31966-021) supplemented with 10% fetal bovine serum were seeded in each well of 12-well plates ...
-
bioRxiv - Biochemistry 2021Quote: HEK293 cells were cultured in Dulbecco’s modified Eagle medium (Gibco) supplemented with 10% (v/v ...
-
bioRxiv - Biochemistry 2021Quote: ... Dulbecco’s Modified Eagle’s Medium for HEK293 cells) (Gibco, Thermofisher Scientific) containing 10% (v/v ...
-
bioRxiv - Biochemistry 2021Quote: ... Dulbecco’s Modified Eagle’s Medium for HEK293 cells) (Gibco, Thermofisher Scientific) containing 10% (v/v ...
-
bioRxiv - Molecular Biology 2021Quote: HEK293-6E cells were transfected using 293fectin protocol (Thermo Fisher). The SIRT6 biosensors were expressed using mammalian expression vector pTT5 (National Research Council ...
-
bioRxiv - Microbiology 2021Quote: ... and RaTG13 RBD were produced using HEK293-F cells (Invitrogen) cultivated in suspension using Freestyle medium (Invitrogen ...
-
bioRxiv - Immunology 2022Quote: ... HEK293 and COS-7 cells and Lipofectamine 2000 (Thermo Fisher) reagent for NIH3T3 cells ...
-
bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were maintained in DMEM (Gibco™, #31966-021) supplemented with 10% FBS and 1X Non-essential Amino Acid supplement (Sigma ...
-
bioRxiv - Physiology 2022Quote: ... HEK293 were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS; ...
-
bioRxiv - Neuroscience 2023Quote: HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% fetal bovine Serum (Gibco ...
-
bioRxiv - Cell Biology 2023Quote: HEK293 cells were cultured in Gibco DMEM (ThermoFisher Scientific, 10566024) supplemented with 10% FBS at 37°C with 5% CO2 ...
-
bioRxiv - Microbiology 2022Quote: ... HEK293 cells stably expressing the ecdysone receptor (EcR-293; Invitrogen), and derived cell lines stably expressing GFP-tagged wild-type or dominant negative (E228Q ...
-
bioRxiv - Neuroscience 2023Quote: ... HEK293 transfections were performed with Lipofectamine 2000 reagent (ThermoFisher, #11668019) according to standard procedures and the cells expressing SorLA mutants and TNFα-GFP (or GFP ...
-
bioRxiv - Molecular Biology 2023Quote: HEK293 cells were grown in Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% fetal bovine serum ...
-
bioRxiv - Systems Biology 2023Quote: HEK293 cells were grown and maintained in DMEM (Thermo Fisher), supplemented with 10% FBS (PAN-Biotech) ...
-
bioRxiv - Cell Biology 2023Quote: ... HEK293 and Hela cells were cultured in DMEM media (Invitrogen) supplemented with 10% FBS (Sigma) ...
-
bioRxiv - Developmental Biology 2023Quote: ... HEK293 and P19 cells were transfected by Lipo2000 (Invitrogen, 11668500) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2022Quote: ... suspension HEK293 host cell line (Freestyle 293-F, ThermoFisher Scientific) was grown in Freestyle 293 expression medium (ThermoFisher Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... HEK293 Flp-In™ T-REx™ cells (Thermo Fisher) were co-transfected with the final plasmid and the pOG44 plasmid (Thermo Fisher) ...
-
bioRxiv - Cell Biology 2023Quote: ... was transduced into HEK293 cells using Lipofectamine LTX (Invitrogen-ThermoFisher) to knock out PML by the CRISPR-Cas9 system ...