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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Sialic Acid Binding Ig Like Lectin 5 SIGLEC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... high-binding 96-well microtiter plates (MaxiSorp, Nunc) were coated with total T ...
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bioRxiv - Biochemistry 2023Quote: ... Mouse α-TATA Binding Protein (ThermoFisher 49-1036), Goat α-mouse:HRP (Novus Biologicals NBP2-31347H) ...
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bioRxiv - Immunology 2023Quote: 96-well high-binding microtiter plates (ThermoFisher Scientific) were coated overnight with ricin toxin (0.1 μg/well in PBS ...
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bioRxiv - Physiology 2019Quote: ... Cardiac perfusion with DiIC18(5)-DS (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindodicarbocyanine-5,5’-Disulfonic Acid; Thermo Fisher Scientific) diluted in 4% paraformaldehyde (PFA ...
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bioRxiv - Plant Biology 2020Quote: ... nucleic acid staining was performed by vaccum-infiltrating a 5 μM of Sytox Orange (Thermo Fisher; S11368) solution ...
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bioRxiv - Molecular Biology 2021Quote: 0.63 mmole (1 equivalent) of the nucleoside-5’-monophosphate free acid (Santa Cruz Biotechnology or ACROS Organics) and 5 equivalents of 2-aminoimidazole hydrochloride (Combi-Blocks ...
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bioRxiv - Immunology 2022Quote: ... resuspended in pre-sort buffer containing 3-5 nM SYTOX Green Nucleic Acid Stain (Thermo Fisher Scientific), and incubated for 20 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... diluted 5% volume to volume in paraffin oil (Fluka Analytical, 76235) and acetic acid (Fisher Scientific, A385) diluted in 5% volume to volume in water — using a stimulus controller (Ockenfels Syntech ...
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bioRxiv - Synthetic Biology 2020Quote: 0.63 mmole (1 equivalent) of the nucleoside-5’-monophosphate free acid (Santa Cruz Biotechnology or ACROS Organics) and 5 equivalents of 2-aminoimidazole hydrochloride (Combi-Blocks ...
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bioRxiv - Bioengineering 2022Quote: ... all syntheses were cleaved from resin with 5 mL of 92.5:2.5:2.5:2.5 trifluoroacetic acid (>95%, Fisher Scientific):DI water:triisopropylsilane (98% ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% sodium dodecyl sulfate and 5 mM ethylenediaminetetraacetic acid with protease and phosphatase inhibitors (Thermo Fisher Scientific). Cell lysates were centrifuged at 500 g for 30 minutes at 4°C to remove cellular debris ...
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bioRxiv - Biochemistry 2023Quote: ... and 5 μM diethyldithiocarboxamic acid ammonium salt (all reagents from either Thermo Fisher Scientific or Sigma Aldrich). CMH aliquots were stored at -20°C until use ...
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bioRxiv - Molecular Biology 2024Quote: ... The digestion was quenched with 5% formic acid and desalted either using C18 tips (Thermo Fisher 87782) or equal mix of Sera-Mag Carboxylate SpeedBeads (Cytiva 65152105050250 & 45152105050250)100 ...
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bioRxiv - Biophysics 2024Quote: ... cells were harvested using premixed Earle’s balanced salt solution with 5 mM ethylenediaminetetraacetic acid (EDTA) (Life Technologies) and centrifuged at 3000 rpm for 10 min at 21 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... The Alexa Fluor 488 conjugate of lectin PNA (1:400 dilution, L21409, Thermo Fisher Scientific, Shanghai, China) and MitoTracker Deep Red 633 (1:1500 dilution ...
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bioRxiv - Developmental Biology 2022Quote: ... and Alexa Fluor 488-conjugated lectin PNA from Arachis hypogaea was used to visualize acrosomes (L21409, Invitrogen).
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bioRxiv - Microbiology 2022Quote: ... Biotinylated lectins were precomplexed (on ice, 20 min) with 0.2 µg streptavidin Alexa Fluor 488 (S32354, Invitrogen). For titration experiments ...
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bioRxiv - Cell Biology 2021Quote: RBM20 WT and RM20-R636S direct transcript binding sites were determined using the same RBM20 polyclonal antibody (ThermoFisher, Cat#PA5-58068) on WT or R636S HMZ WTC iPS CMs using the eCLIP protocol as previously described (Van Nostrand et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Antibody binding was detected using the enhanced chemiluminescence immunoblotting detection system (Perkin Elmer LLC WESTERN LIGHTNING PLUS-ECL, Thermo Fisher Scientific) and Kodak autoradiography film ...
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bioRxiv - Immunology 2020Quote: The Fab fragments for anti-SARS-CoV-2 antibodies ION_300 and ION_360 and SARS-CoV-2 receptor binding domain (RBD) were expressed in Expi293F™ cells (Thermo Fisher, A14527). The antibody:RBD complexes were mixed and co-purified by size exclusion chromatography in 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibody binding was visualized using species specific fluorescent secondary antibodies conjugated to Alexa Fluor goat anti-mouse 488 (1:1000; Molecular Probes). Sections were mounted using fluorescent mounting medium (DAKO).
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bioRxiv - Biophysics 2019Quote: ... HAUS8 (MT binding subunit of augmin complex)-and rabbit IgG(control)-antibody were conjugated to Protein A Dynabeads (Life Technologies #10002D) overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Interactions were visualized by the incubation of tetramethylrhodamine (TRITC) labelled secondary goat anti-mouse IgG antibodies (Fc specific; 1:1000 dilution in binding buffer; Life Technologies) and goat anti-Human IgG (Fc specific)-Cy3 (1:1000 dilution in binding buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... and labeled on ice with 1:250 dilution of secondary antibodies for binding (anti-mouse 488, ThermoFisher A11059 or anti-human 647, ThermoFisher A21445) and expression (anti-chicken 647 ...
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bioRxiv - Microbiology 2023Quote: ... Samples were subsequently incubated with 10 % BSA to block the unspecific binding followed by incubation with a secondary α-rabbit antibody conjugated to Alexa Fluor 488 (Invitrogen) and diluted 1:1,000 (in PBS) ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell binding experiment was done with the human T lymphocyte cell Jurkat and anti-human IgG antibody-Ax488 (ThermoFisher Scientific). Ax488 signal changes upon binding were detected using a flow cytometer (BD Accuri C6 ...
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bioRxiv - Bioengineering 2023Quote: Antigens for differential phage panning or antibody binding in IgG format were expressed by transient transfection of BirA-Expi293 cells (Life Technologies) with plasmids encoding CDCP1 ...
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bioRxiv - Microbiology 2021Quote: ... followed by its secondary staining (donkey anti-goat Ig Alexa 488, A100555, Invitrogen, 1:300), then primary staining for mCherry (rabbit anti-DsRed ...
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bioRxiv - Microbiology 2020Quote: ... The Ig PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Thermo Fisher) in a total volume of 50 µl ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... diluted at 1:100 and secondarily with Alexa 488-congugated goat anti-rabbit Ig (Invitrogen) diluted at 1:400 ...
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Co-stimulatory molecules decide T cell fate through regulations of their invigoration and impairmentbioRxiv - Molecular Biology 2022Quote: ... or PDL1-Ig were cultured in RPMI1640 supplemented with 10% Ultra Low IgG FBS (GIBCO). Culture supernatants were collected and extracted by Sepharose beads (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... IA) with the top ranking promoter-like elements altered by synonymous mutations and cloned into pCR4-Blunt (Invitrogen). Bacterial clones were screened by restriction digestion and those with correct patterns were analyzed by DNA sequencing ...
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bioRxiv - Cell Biology 2019Quote: ... For nuclei staining HL-60 neutrophil-like cells were treated with 0.5□μg/ml Hoechst 33342 (Life Technologies) for 5□minutes at 37□°C and washed in PBS once ...
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bioRxiv - Cell Biology 2020Quote: 7000 Monkey kidney fibroblast-like COS-7cells were seeded per well in gelatin-coated 96- well plates (Gibco DMEM ...
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bioRxiv - Microbiology 2021Quote: J774.1 mouse macrophage-like cells (ECACC, Salisbury, UK) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher) supplemented with 200 U/ml penicillin/streptomycin ...
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bioRxiv - Immunology 2023Quote: Human embryonic kidney (HEK) epithelial-like cell line HEK293T was maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (11965-118; GIBCO™ ...
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bioRxiv - Biophysics 2020Quote: ... and ligand displacement data were fit to the “one site – Fit Ki” binding equation to obtain Ki values using the published binding affinity of Fluormone Pan-PPAR Green (2.8 nM; Invitrogen PV4894 info sheet).
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bioRxiv - Biophysics 2019Quote: ... and ligand displacement data were fit to the one site – Fit Ki binding equation to obtain Ki values using the known binding affinity of Fluormone Pan-PPAR Green (2.8 nM; Invitrogen PV4894 product insert).
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bioRxiv - Immunology 2020Quote: ... or 5 μg Wapl antibody (Thermo Fisher Scientific, #PA5-38024) overnight at 4 °C ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Tau monoclonal antibody (Tau-5) (Thermo Fisher Scientific, Waltham, MA), and Actin (MP Biomedicals ...
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bioRxiv - Immunology 2023Quote: ... Antibodies used were: anti-CD4 APC-Alexa647 (RM4-5, ThermoFisher), anti-CD8 BUV805 (BD 53-6.7 ...
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bioRxiv - Biochemistry 2023Quote: ... 5 µg of monoclonal DNA antibody (Cat# MA1-83116; Invitrogen) was incubated for 2 hrs at 4 °C with Protein A/G Mix Magnetic Beads (Cat# LSKMAGAG10 ...
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bioRxiv - Plant Biology 2022Quote: ... an additional cleaning step with acid phenol was added before precipitation of the RNA by adding an equal volume of Acid-Phenol:Chloroform 5:1 solution pH 4.5 (Ambion). Total RNA was quantified with a Nanodrop 2000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... DNA was stained with Sytox green nucleic acid stain (5 mM solution in DMSO from Thermo Fisher Scientific) and analyzed on a BD Accuri flow cytometer.
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bioRxiv - Cell Biology 2019Quote: ... for 10 minutes, and fixed with methanol:glacial acetic acid, (5:1, v/v) (FisherThermo Fisher Scientific, Rockford, IL). Cells were dropped onto slides ...
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bioRxiv - Genetics 2020Quote: ... and in some cases day 5 (120 hours) by staining with SYBR Green 1 nucleic acid stain (Invitrogen, ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... were harvested in an Eppendorf tube and resuspended in 0.5 mL NaCl (0.85%) containing SYTO9 green-fluorescent nucleic acid stain (5 nM final concentration, Invitrogen). Following incubation in the dark for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by binding to Protein-G Dynabeads (Invitrogen, 10003D) for 20 minutes hours at 4°C to enrich for endothelial cell nuclei ...
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bioRxiv - Immunology 2021Quote: High binding ELISA plates (07-200-37, Fisher Scientific) were coated with 1 μg/mL of labeled and blocked with 2% BSA in PBS ...
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bioRxiv - Immunology 2021Quote: ... high binding ELISA plates (07-200-37, Fisher Scientific) were coated with 1 μg/mL trimer and blocked with 2% BSA in PBS ...