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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for Sialic Acid Binding Ig Like Lectin 5 SIGLEC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... and proteins were separated using a gradient of solvent B (99% acetonitrile with 0.1% formic acid) from 5 to 65% in 90 minutes using an EASY-nLC 1000 system (Thermo Fisher Scientific). Ion fragmentation was conducted using collision-induced dissociation (CID) ...
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bioRxiv - Cell Biology 2021Quote: ... the peptides were resuspended in 3% formic acid (FA)/5% acetonitrile (ACN) and loaded onto a nanoLC system (RSLCnano, Thermo Scientific). First ...
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bioRxiv - Plant Biology 2022Quote: SepPak eluted peptides were topped up to 65% (v/v) acetonitrile (ACN) and 5% (v/v) trifluoroacetic acid (TFA) prior to enrichment using a KingFisher Duo (Thermo Scientific) automated sample preparation device as outlined by Leutert et al ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with 0.5 µM Lipi-Deep Red neutral lipid stain (Dojindo #LD04-10) for 2 hr and 5 µg/mL Hoeschst 33342 nucleic acid stain (Invitrogen #H3570) for 30 min at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... with 0.05% (v/v) trifluoroacetic acid (5 min 20 µL/min) using split-free UltiMate 3000 RSLCnano HPLCs (Dionex / Thermo Scientific, Germany). Bound peptides were then eluted with an aqueous-organic gradient (eluent A ...
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bioRxiv - Microbiology 2022Quote: ... Amino acids were extracted on ice-cold lysis buffer [5:3:2 ratio of methanol-acetonitrile-water (Fisher Scientific, Pittsburgh, PA)] containing 3 µM of amino acid standards [Cambridge Isotope Laboratories ...
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bioRxiv - Plant Biology 2019Quote: The peptides were dissolved in 5 μL of 0.3% formic acid with 3% acetonitrile and injected into an Easy-nLC 1200 (Thermo Fisher Scientific). Peptides were separated on a 25 cm Easy-Spray column (75 μm ID ...
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bioRxiv - Neuroscience 2022Quote: ... Eluted peptides were dried down overnight before resuspension in 5% formic acid ready for injection onto the Orbitrap Exploris (Thermo Fisher) mass spectrometer using data independent acquisition (DIA) ...
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bioRxiv - Immunology 2022Quote: ... gels were stained by incubation in 50 ml ddH20 containing 5 μl SYBR® Gold Nucleic Acid Gel Stain (Invitrogen, S11494) on a rocking platform for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... slices were transferred into a sealed 1.5 ml tube containing 5 µM Fluo-4AM (Molecular Probes, 50% DMSO, 50% Pluronic Acid) diluted in oxygenated modified artificial cerebrospinal fluid (mACSF ...
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bioRxiv - Biochemistry 2023Quote: ... DNA migration was detected by a 5 min incubation with SYBR Gold Nucleic Acid Gel Stain (Invitrogen #S11494, 1/10, 000) and revealed using a Chemidoc MP Immaging System (BIO-RAD) ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was acidified with formic acid (5% final concentration) and fractionated using high pH reversed-phase peptide fractionation kits (ThermoFisher, 84868) according to Vinogradova and coworkers (Cell ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 g/L ammonium sulphate (Fisher Chemical™) and 1.7 g/L yeast nitrogen base without amino acids (Thermo Scientific™) and was supplemented with 2% (w/v ...
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bioRxiv - Cell Biology 2023Quote: For flowcytometric quantification of GFP signal in induced 3D7-DiCre pFIO/pFIO+ strains 2.5 µl iRBCs were washed once with RT 0.9% NaCl solution (B. Braun GmbH) and stained with 5 µM SYTO 61 Red Fluorescent Nucleic Acid stain (Thermo Fisher Scientific) for 1 h at 37°C in 0.9% NaCl solution and washed once again afterwards at RT ...
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bioRxiv - Immunology 2023Quote: ... A 5-fold excess of pre-warmed RPMI+ medium (RPMI-1640, 100 μM non-essential amino acids, 20 mM HEPES [Thermo Fisher Scientific] ...
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bioRxiv - Microbiology 2023Quote: ... The dry pooled sample was resuspended in 1 ml of 5% formic acid and was desalted using the EasyPep Maxi Kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... protocol and these dry peptides residues were solubilized in 20 µL 5 % acetonitrile with 0.5 % formic acid using the auto-sampler of a nanoflow uHPLC system (Thermo Scientific RSLCnano). Online detection of peptide ions was by electrospray ionisation (ESI ...
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bioRxiv - Cancer Biology 2024Quote: ... in the extracellular domain of the CaSR at a concentration of 5 μg/ml and counterstained with SYTO13 fluorescent nucleic acid dye (ThermoFisher, S7575) as previously described 33 ...
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bioRxiv - Microbiology 2023Quote: ... 20% volume of 1M 2-morpholin-4-ylethanesulfonic acid (MES) pH 5 and Immobilized Protein G resin (Thermo Scientific Prod#20397) were added to supernatant and sample was incubated overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... 1 × TE buffer (5 mM Tris base, pH 8.0 and 1 mM EDTA acid) containing 15% w/v PEG-8000 (Fisher Scientific, BP2331) and 510 mM NaCl was added to the SQB sample at 1:1 volume and mixed gently via pipetting ...
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bioRxiv - Cancer Biology 2024Quote: ... Each sample was then resuspended in a volume of sample LC/MS loading solvent [5% formic acid (Optima grade, ThermoFisher Scientific) and 4% acetonitrile (both water and acetonitrile were LC-MS-grade ...
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bioRxiv - Cell Biology 2021Quote: ... and a mix of amino-acids (1X MEM Non-Essential Amino Acids and 1X MEM Amino Acids, both manufactured by Gibco R). For strains and plasmid construction cells were grown in LB ...
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bioRxiv - Cancer Biology 2019Quote: ... 4 mM serine and essential amino acids (MEM Amino Acids Solution; Gibco, 11130036); for all other experiments ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Hydrochloric acid and acetic acid were provided by Fisher Scientific (Loughborough, United Kingdom). 17α-methyltestosterone was delivered by Steraloids (Newport ...
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bioRxiv - Neuroscience 2021Quote: ... Activated/Cleaved Caspase-3 (1:1000; Cell Signalling Biology, CAT#: 9661S and Thermo Fisher Scientific, CAT#: 66470-2-IG), Nestin (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... USA) were transfected with 2 plasmids: B45 encoding gp96-Ig (source: University of Miami) and pcDNA™ 3.1(-) (Invitrogen), encoding full-length SARS-CoV-2 protein S gene (Genomic Sequence ...
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bioRxiv - Microbiology 2022Quote: ... the concentration of biotinylated Igs after desalting was measured at 280 nm via spectrophotometer (Nanodrop ND-1000, ThermoFisher Scientific); biotin incorporation was measured using the Pierce Biotin quantitation kit (ThermoFisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... incubated for 5 min with 25 pM (single-label) or 5 pM (dual-label) biotin-tagged α-FLAG monoclonal antibody (ThermoFisher Scientific #MA1-91878-BTIN ...
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bioRxiv - Microbiology 2020Quote: ... burgdorferi strains grown under mammalian-like conditions were used for reverse transcription into cDNA using random primers (Thermo Fisher Scientific, Waltham, MA) and SuperScript III (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... burgdorferi strains grown under mammalian-like conditions were used for reverse transcription into cDNA using random primers (Thermo Fisher Scientific, Waltham, MA) and SuperScript III (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... PMPs were harvested from suspension during medium exchange and plated in microglia differentiation media over 7-14 days to produce microglia like cell monocultures (Neurobasal (Life Technologies 21103049) supplemented with Gem21 NeuroPlex without Vitamin A (GeminiBio ...
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bioRxiv - Molecular Biology 2022Quote: ... Phasi Charoen like virus and Culex Y virus) were maintained at 28 °C in Leibovitz’s L-15 medium (Invitrogen: catalogue number: 21083027) supplemented with 10% foetal bovine serum (Gibco) ...
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bioRxiv - Microbiology 2021Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... mucus-like compounds were stained by incubation (overnight at 4°C) with AlexaFluor 647-labeled PNA (1:1000 in PBSt; Molecular Probes #L32460).
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bioRxiv - Molecular Biology 2023Quote: ... macrophage precursors in the media supernatant were harvested weekly and differentiated into microglia-like cells using advanced DMEM/F12 supplemented with N2 (Thermofisher, 17502-048), 100 ng/mL IL-34 (Peprotech ...
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bioRxiv - Biochemistry 2024Quote: ... Human neutrophil-like HL-60 cells were gifted by Orion Weiner (UCSF) and grown in supplemented RPMI medium 1640 (Gibco #22400-089) as described previously 28,54 ...
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bioRxiv - Cell Biology 2020Quote: ... followed by addition of antibodies diluted in 5% horse serum (ThermoFisher Scientific, 26050070) in PBS ...
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bioRxiv - Physiology 2020Quote: ... 5 μg rabbit IgG or PTBP1 Antibody (Cat. No. 32-4800, FISHER SCIENTIFIC) were first incubated with 30 μl washed Dynabeads® Protein G in 300 μl RIP buffer supplemented with 0.2 mg/ml BSA ...
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bioRxiv - Molecular Biology 2022Quote: ... Antibodies (5 µg each) used for RIP were: Rabbit IgG (Invitrogen, #02-6102), Fibrillarin (Abcam ...
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bioRxiv - Developmental Biology 2022Quote: ... and H1-G2E1-5 was detected by mouse anti-His6-tag antibody (Invitrogen) and subsequently by HRP-conjugated secondary antibody (Jackson ImmunoResearch) ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies: Claudin-5 (mouse, clone 4C3C2, ThermoFisher cat# 35-2500, 1:100), Iba1 (goat polyclonal ...
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bioRxiv - Cell Biology 2019Quote: ... All antibodies were diluted in 5% BSA and 0.1% Tween-20 (Fisher Scientific). Primary antibodies were incubated at 4°C overnight and secondary antibodies (Li-Cor ...
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bioRxiv - Neuroscience 2019Quote: ... Biotinylated IgG secondary antibody was used at 5 μg / ml (Invitrogen, Carlsbad, CA) for 2-hour incubations ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μg of antibody coupled to 2 ug of magnetic Dynabeads (Life Technologies) were added to 3 mL of sonicated nuclear extract from formaldehyde fixed cells ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μg of antibody coupled to 2 μg of magnetic Dynabeads (Life Technologies) was added to 3 ml of sonicated nuclear extract from formaldehyde-fixed cells ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μg of antibody coupled to 2 μg of magnetic Dynabeads (Life Technologies) was added to 3 mL of sonicated nuclear extract from formaldehyde-fixed cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Anti-CD4 (RM4-5) and anti-NKp46 (PA5-102860) antibodies were from ThermoFisher. Anti-CD68 and anti-DC-SIGN/CD209 antibodies were from GeneTex ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 ug of total BMP6 monoclonal antibody (Thermo Fisher Scientific, Cat# MA5-23844) was added to the culture medium for 48hrs and cells were then harvested for protein analysis ...
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bioRxiv - Biochemistry 2023Quote: ... Fluorescently labelled detection antibodies (AT8: Invitrogen, Cat. No. MN1020, 5 nM; HT7: Invitrogen, Cat ...